AU2003216830A1 - Purification methods for oligonucleotides and their analogs - Google Patents

Purification methods for oligonucleotides and their analogs

Info

Publication number: AU2003216830A1
Authority: AU; Australia
Prior art keywords: oligonucleotides; analogs; purification methods; purification; methods
Prior art date: 2002-03-21
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

AU2003216830A

Inventor

Jack T. Johansen

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Nitto Denko Avecia Inc

Original Assignee

Avecia Biotechnology Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2002-03-21

Filing date

2003-03-19

Publication date

2003-10-08

2003-03-19 Application filed by Avecia Biotechnology Inc filed Critical Avecia Biotechnology Inc

2003-10-08 Publication of AU2003216830A1 publication Critical patent/AU2003216830A1/en

Status Abandoned legal-status Critical Current

Links

108091034117 Oligonucleotide Proteins 0.000 title 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
238000000034 method Methods 0.000 title 1
238000000746 purification Methods 0.000 title 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Crystallography & Structural Chemistry (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

AU2003216830A 2002-03-21 2003-03-19 Purification methods for oligonucleotides and their analogs Abandoned AU2003216830A1 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US36706002P	2002-03-21	2002-03-21
US60/367,060		2002-03-21
PCT/GB2003/001161 WO2003080834A2 (en)	2002-03-21	2003-03-19	Purification methods for oligonucleotides and their analogs

Publications (1)

Publication Number	Publication Date
AU2003216830A1 true AU2003216830A1 (en)	2003-10-08

Family

ID=28454833

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AU2003216830A Abandoned AU2003216830A1 (en)	2002-03-21	2003-03-19	Purification methods for oligonucleotides and their analogs

Country Status (7)

Country	Link
EP (1)	EP1490488A2 (en)
JP (1)	JP2005520547A (en)
KR (1)	KR20040108672A (en)
CN (1)	CN1643145A (en)
AU (1)	AU2003216830A1 (en)
CA (1)	CA2479901A1 (en)
WO (1)	WO2003080834A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2006132588A1 (en) *	2005-06-10	2006-12-14	Quiatech Ab	Method for the purification of synthetic oligonucleotides containing one or several labels
DE102005059315A1 (en) *	2005-12-09	2007-06-14	Qiagen Gmbh	Process for the enrichment of short-chain nucleic acids
AU2012205204B2 (en) *	2005-12-09	2015-03-12	Qiagen Gmbh	Method for enriching short-chain nucleic acids
GB2443505B (en)	2006-09-26	2008-12-31	Ge Healthcare Bio Sciences	Nucleic acid purification method
GB2445441B (en)	2006-09-26	2010-06-30	Ge Healthcare Bio Sciences	Nucleic acid purification method
GB2445442A (en)	2006-09-26	2008-07-09	Ge Healthcare Bio Sciences	Nucleic acid purification using anion exchange
DE102008063003A1 (en)	2008-12-23	2010-06-24	Qiagen Gmbh	Nucleic acid purification method
JP5914338B2 (en) *	2009-09-24	2016-05-11	キアジェンゲイサーズバーグインコーポレイテッド	Compositions, methods and kits for nucleic acid isolation and analysis using anion exchange materials
CN102753705A (en) *	2009-12-14	2012-10-24	B·吴	Method and materials for separating nucleic acid materials
EP2760999A1 (en) *	2011-09-26	2014-08-06	Qiagen GmbH	Methods for separating nucleic acids by size
CN103833797A (en) *	2012-11-26	2014-06-04	福建金山生物制药股份有限公司	Industrial method for purifying phosphorothioate oligodeoxynucleotide
US10745686B2 (en)	2013-02-08	2020-08-18	Qiagen Gmbh	Method for separating DNA by size
CN106632558B (en) *	2016-08-25	2019-09-13	淮阴师范学院	A kind of purification method and application of oligonucleotide
CA3110524A1 (en)	2018-09-11	2020-03-19	Amgen Inc.	Purification methods for guanine-rich oligonucleotides
EP3997100A1 (en) *	2019-07-09	2022-05-18	F. Hoffmann-La Roche AG	Process for the deprotection of oligonucleotides
EP4070102A1 (en) *	2019-12-05	2022-10-12	Waters Technologies Corporation	Polyphosphonic acids for improving recovery and minimizing system loss
CN111394427B (en) *	2020-04-22	2023-04-11	宁波市博坤生物科技有限公司	Method for extracting trace DNA in soil-containing test material
CN116209757A (en) *	2020-07-14	2023-06-02	沃特世科技公司	Compositions, kits and methods for separating oligonucleotides from matrix components
WO2024053578A1 (en)	2022-09-07	2024-03-14	株式会社日本触媒	Nucleic acid production method, method for removing impurities, and nucleic acid having less impurities
JPWO2024089953A1 (en) *	2022-10-27	2024-05-02
EP4556572A1 (en) *	2023-11-20	2025-05-21	Sartorius BIA Separations d.o.o.	Method for purifying one or more target nucleic acid(s)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0755400A1 (en) *	1994-04-08	1997-01-29	HYBRIDON, Inc.	Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
US6310199B1 (en) *	1999-05-14	2001-10-30	Promega Corporation	pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
GB9927904D0 (en) *	1999-11-25	2000-01-26	Amersham Pharm Biotech Ab	A method fro obtaining a nucleic acid variant
DE10010342A1 (en) *	2000-03-06	2001-09-20	Merck Patent Gmbh	Method for reducing the endotoxin content of nucleic acid (I) is derived from natural, genetic engineering or biotechnological sources is used to produce high-purity plasmid DNA from natural sources

2003
- 2003-03-19 WO PCT/GB2003/001161 patent/WO2003080834A2/en not_active Application Discontinuation
- 2003-03-19 KR KR10-2004-7014747A patent/KR20040108672A/en not_active Withdrawn
- 2003-03-19 CA CA002479901A patent/CA2479901A1/en not_active Abandoned
- 2003-03-19 JP JP2003578560A patent/JP2005520547A/en active Pending
- 2003-03-19 EP EP03712366A patent/EP1490488A2/en not_active Withdrawn
- 2003-03-19 AU AU2003216830A patent/AU2003216830A1/en not_active Abandoned
- 2003-03-19 CN CNA03806393XA patent/CN1643145A/en active Pending

Also Published As

Publication number	Publication date
CN1643145A (en)	2005-07-20
KR20040108672A (en)	2004-12-24
WO2003080834A2 (en)	2003-10-02
CA2479901A1 (en)	2003-10-02
JP2005520547A (en)	2005-07-14
EP1490488A2 (en)	2004-12-29
WO2003080834A3 (en)	2003-12-31

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Legal Events

Date	Code	Title	Description
2004-12-09	MK6	Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase