AU2002356891A1 - Method and system for inducible recombinational cloning in bacterial cells - Google Patents
Method and system for inducible recombinational cloning in bacterial cellsInfo
- Publication number
- AU2002356891A1 AU2002356891A1 AU2002356891A AU2002356891A AU2002356891A1 AU 2002356891 A1 AU2002356891 A1 AU 2002356891A1 AU 2002356891 A AU2002356891 A AU 2002356891A AU 2002356891 A AU2002356891 A AU 2002356891A AU 2002356891 A1 AU2002356891 A1 AU 2002356891A1
- Authority
- AU
- Australia
- Prior art keywords
- inducible
- bacterial cells
- recombinational cloning
- recombinational
- cloning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000001580 bacterial effect Effects 0.000 title 1
- 238000010367 cloning Methods 0.000 title 1
- 230000001939 inductive effect Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US33091001P | 2001-11-02 | 2001-11-02 | |
| US60/330,910 | 2001-11-02 | ||
| PCT/US2002/035217 WO2003040318A2 (en) | 2001-11-02 | 2002-11-04 | Method and system for inducible recombinational cloning in bacterial cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2002356891A1 true AU2002356891A1 (en) | 2003-05-19 |
Family
ID=23291829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2002356891A Abandoned AU2002356891A1 (en) | 2001-11-02 | 2002-11-04 | Method and system for inducible recombinational cloning in bacterial cells |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2002356891A1 (en) |
| WO (1) | WO2003040318A2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845445B (en) * | 2010-02-04 | 2012-05-09 | 东北农业大学 | Cre recombinase gene and Cre/loxP-mediated transgenic safety plant expression vector |
| CN108642086B (en) * | 2018-05-14 | 2022-06-07 | 北京工业大学 | High-sensitivity fusion cell screening method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5928914A (en) * | 1996-06-14 | 1999-07-27 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | Methods and compositions for transforming cells |
| AU722375B2 (en) * | 1996-09-06 | 2000-08-03 | Trustees Of The University Of Pennsylvania, The | Methods using cre-lox for production of recombinant adeno-associated viruses |
| DE19650714A1 (en) * | 1996-12-06 | 1998-06-10 | Melchner Harald Von Prof Dr | Gene trap construct for the identification and isolation of genes |
| DE69836092T2 (en) * | 1997-10-24 | 2007-05-10 | Invitrogen Corp., Carlsbad | RECOMBINATORY CLONING USING NUCLEAR FACILITIES WITH RECOMBINATION CENTERS |
| EP1114148A4 (en) * | 1998-08-28 | 2004-12-22 | Invitrogen Corp | System for the rapid manipulation of nucleic acid sequences |
-
2002
- 2002-11-04 AU AU2002356891A patent/AU2002356891A1/en not_active Abandoned
- 2002-11-04 WO PCT/US2002/035217 patent/WO2003040318A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003040318A3 (en) | 2003-11-06 |
| WO2003040318A2 (en) | 2003-05-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase |