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AU2001284295A1 - Method of sequencing dna - Google Patents

Method of sequencing dna

Info

Publication number
AU2001284295A1
AU2001284295A1 AU2001284295A AU8429501A AU2001284295A1 AU 2001284295 A1 AU2001284295 A1 AU 2001284295A1 AU 2001284295 A AU2001284295 A AU 2001284295A AU 8429501 A AU8429501 A AU 8429501A AU 2001284295 A1 AU2001284295 A1 AU 2001284295A1
Authority
AU
Australia
Prior art keywords
nucleotide
base
analogue
target position
incorporated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001284295A
Inventor
Mostafa Ronaghi
Annika Tallsjo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotage AB
Original Assignee
Pyrosequencing AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pyrosequencing AB filed Critical Pyrosequencing AB
Publication of AU2001284295A1 publication Critical patent/AU2001284295A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention provides a method of identifying a base at a target position in a sample nucleic acid sequence, said method comprising: subjecting a primer hybridised to said sample nucleic acid immediately adjacent to the target position, to a polymerase primer extension reaction in the presence of a nucleotide, whereby the nucleotide will only become incorporated if it is complementary to the base in the target position, and determining whether or not said nucleotide is incorporated by detecting whether PPi is released, the identity of the target base being determined from the identity of any nucleotide incorporated, wherein, where said nucleotide comprises an adenine base, an alpha-thio triphosphate analogue of said nucleotide is used, ant the Rp isomer of said analogue and/or the degradation products of said analogue are eliminated from the polymerase reaction step.
AU2001284295A 2000-09-07 2001-09-07 Method of sequencing dna Abandoned AU2001284295A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0021977.4 2000-09-07
GBGB0021977.4A GB0021977D0 (en) 2000-09-07 2000-09-07 Method of sequencing DNA
PCT/GB2001/004015 WO2002020836A2 (en) 2000-09-07 2001-09-07 Method of sequencing dna

Publications (1)

Publication Number Publication Date
AU2001284295A1 true AU2001284295A1 (en) 2002-03-22

Family

ID=9899042

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001284295A Abandoned AU2001284295A1 (en) 2000-09-07 2001-09-07 Method of sequencing dna

Country Status (10)

Country Link
US (2) US7459311B2 (en)
EP (1) EP1317568B1 (en)
JP (1) JP4271439B2 (en)
AT (1) ATE395435T1 (en)
AU (1) AU2001284295A1 (en)
CA (1) CA2421492C (en)
DE (1) DE60134030D1 (en)
ES (1) ES2305099T3 (en)
GB (1) GB0021977D0 (en)
WO (1) WO2002020836A2 (en)

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Also Published As

Publication number Publication date
CA2421492C (en) 2010-05-04
US7459311B2 (en) 2008-12-02
WO2002020836A2 (en) 2002-03-14
CA2421492A1 (en) 2002-03-14
US20090155801A1 (en) 2009-06-18
DE60134030D1 (en) 2008-06-26
EP1317568A2 (en) 2003-06-11
JP2004508054A (en) 2004-03-18
US7648824B2 (en) 2010-01-19
GB0021977D0 (en) 2000-10-25
WO2002020836A3 (en) 2003-02-06
ES2305099T3 (en) 2008-11-01
EP1317568B1 (en) 2008-05-14
US20040142330A1 (en) 2004-07-22
JP4271439B2 (en) 2009-06-03
ATE395435T1 (en) 2008-05-15

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