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AU2001274428A1 - Method of rna cleavage - Google Patents

Method of rna cleavage

Info

Publication number
AU2001274428A1
AU2001274428A1 AU2001274428A AU7442801A AU2001274428A1 AU 2001274428 A1 AU2001274428 A1 AU 2001274428A1 AU 2001274428 A AU2001274428 A AU 2001274428A AU 7442801 A AU7442801 A AU 7442801A AU 2001274428 A1 AU2001274428 A1 AU 2001274428A1
Authority
AU
Australia
Prior art keywords
bulge
target molecule
rna cleavage
helix
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001274428A
Inventor
Maria Irene Baldi
Giancarlo Deidda
Paolo Fruscoloni
Nicoletta Rossi
Glauco P. Tocchini-Valentini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consiglio Nazionale delle Richerche CNR
Original Assignee
CNR ISTITUTO DI BIOLOG CELLULA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CNR ISTITUTO DI BIOLOG CELLULA filed Critical CNR ISTITUTO DI BIOLOG CELLULA
Publication of AU2001274428A1 publication Critical patent/AU2001274428A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes

Landscapes

  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A method of cleaving a target RNA molecule is disclosed. In one embodiment the method comprises the step of exposing the target molecule to an eukaryotic tRNA splicing endonuclease, wherein the target molecule is in the bulge-helix-bulge conformation, wherein cleavage occurs within the bulge-helix-bulge and cleavage products are generated, and wherein the target molecule does not comprise a tRNA structure.
AU2001274428A 2000-05-31 2001-05-30 Method of rna cleavage Abandoned AU2001274428A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20843200P 2000-05-31 2000-05-31
US60/208,432 2000-05-31
PCT/IB2001/001189 WO2001092463A2 (en) 2000-05-31 2001-05-30 Method of rna cleavage

Publications (1)

Publication Number Publication Date
AU2001274428A1 true AU2001274428A1 (en) 2001-12-11

Family

ID=22774580

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001274428A Abandoned AU2001274428A1 (en) 2000-05-31 2001-05-30 Method of rna cleavage

Country Status (7)

Country Link
US (1) US20040023239A1 (en)
EP (1) EP1303597B1 (en)
JP (1) JP2003534789A (en)
AT (1) ATE419347T1 (en)
AU (1) AU2001274428A1 (en)
DE (1) DE60137226D1 (en)
WO (1) WO2001092463A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8512945B2 (en) * 2000-05-31 2013-08-20 Glauco P. Tocchini-Valentini Method of RNA cleavage and recombination
WO2004087070A2 (en) * 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. METHODS OF IDENTIFYING COMPOUNDS THAT TARGET tRNA SPLICING ENDONUCLEASE AND USES OF SAID COMPOUNDS AS ANTI-FUNGAL AGENTS
WO2004087069A2 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. METHODS OF IDENTIFYING COMPOUNDS THAT TARGET tRNA SPLICING ENDONUCLEASE AND USES OF SAID COMPOUNDS AS ANTI-PROLIFERATIVE AGENTS
EP1649002A4 (en) * 2003-07-02 2009-06-03 Ptc Therapeutics Inc Rna processing protein complexes and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5869248A (en) * 1994-03-07 1999-02-09 Yale University Targeted cleavage of RNA using ribonuclease P targeting and cleavage sequences
WO1995032283A1 (en) * 1994-05-23 1995-11-30 Indiana University Foundation Sequence-specific endonucleases, and preparation and use of same

Also Published As

Publication number Publication date
WO2001092463A3 (en) 2003-01-09
WO2001092463A2 (en) 2001-12-06
ATE419347T1 (en) 2009-01-15
EP1303597A2 (en) 2003-04-23
JP2003534789A (en) 2003-11-25
EP1303597B1 (en) 2008-12-31
DE60137226D1 (en) 2009-02-12
US20040023239A1 (en) 2004-02-05

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