AU2001268662A1 - Assembly of wild-type and chimeric influenza virus-like particles (VLPs) - Google Patents

Assembly of wild-type and chimeric influenza virus-like particles (VLPs)

Info

Publication number: AU2001268662A1
Authority: AU; Australia
Prior art keywords: vlps; influenza; protein; influenza virus; cells
Prior art date: 2000-06-23
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

AU2001268662A

Other languages

English (en)

Inventor

Jose M. Galarza

Theresa E. Latham

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Wyeth Holdings LLC

Original Assignee

American Cyanamid Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-06-23

Filing date

2001-06-21

Publication date

2002-01-08

2001-06-21 Application filed by American Cyanamid Co filed Critical American Cyanamid Co

2002-01-08 Publication of AU2001268662A1 publication Critical patent/AU2001268662A1/en

2003-09-11 Assigned to WYETH HOLDINGS CORPORATION reassignment WYETH HOLDINGS CORPORATION Amend patent request/document other than specification (104) Assignors: AMERICAN CYANAMID COMPANY

Status Abandoned legal-status Critical Current

Links

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AU2001268662A 2000-06-23 2001-06-21 Assembly of wild-type and chimeric influenza virus-like particles (VLPs) Abandoned AU2001268662A1 (en)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
US21365600P	2000-06-23	2000-06-23
US60/213,656		2000-06-23
US28441101P	2001-04-17	2001-04-17
US60/284,411		2001-04-17
PCT/US2001/019890 WO2002000885A2 (fr)	2000-06-23	2001-06-21	Assemblage sauvage-chimerique de particules viraloïdes de la grippe (vlp)

Publications (1)

Publication Number	Publication Date
AU2001268662A1 true AU2001268662A1 (en)	2002-01-08

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AU2001268662A Abandoned AU2001268662A1 (en)	2000-06-23	2001-06-21	Assembly of wild-type and chimeric influenza virus-like particles (VLPs)
AU2007201727A Expired AU2007201727B2 (en)	2000-06-23	2007-04-18	Assembly of wild-type and chimeric influenza virus-like particles (VLPs)

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Application Number	Title	Priority Date	Filing Date
AU2007201727A Expired AU2007201727B2 (en)	2000-06-23	2007-04-18	Assembly of wild-type and chimeric influenza virus-like particles (VLPs)

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US (2)	US7556940B2 (fr)
EP (1)	EP1294892B1 (fr)
JP (1)	JP5008244B2 (fr)
KR (1)	KR100877019B1 (fr)
CN (1)	CN1196788C (fr)
AT (1)	ATE376059T1 (fr)
AU (2)	AU2001268662A1 (fr)
BR (1)	BRPI0111830B8 (fr)
CA (1)	CA2410297C (fr)
CY (1)	CY1107835T1 (fr)
DE (1)	DE60130987T2 (fr)
DK (1)	DK1294892T3 (fr)
ES (1)	ES2295175T3 (fr)
IL (1)	IL153290A0 (fr)
MX (1)	MXPA02012254A (fr)
PT (1)	PT1294892E (fr)
WO (1)	WO2002000885A2 (fr)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH0250488A (ja) *	1988-08-11	1990-02-20	Nec Corp	超伝導配線
AU2001268662A1 (en) *	2000-06-23	2002-01-08	Wyeth Holdings Corporation	Assembly of wild-type and chimeric influenza virus-like particles (VLPs)
WO2004042001A2 (fr) *	2002-05-17	2004-05-21	Emory University	Particules pseudovirales, procede de fabrication et compositions immunogeniques
US9045727B2 (en)	2002-05-17	2015-06-02	Emory University	Virus-like particles, methods of preparation, and immunogenic compositions
CA2490866A1 (fr) *	2002-06-26	2004-01-08	Advanced Bionutrition Corporation	Virus et particules pseudovirales pour presentation multiple d'antigenes et de cibles
ES2217967B1 (es) *	2003-03-31	2006-01-01	Consejo Sup. Investig. Cientificas	Procedimiento de produccion de particulas virales vacias (vlps) del virus inductor de la bursitis infecciosa (ibdv), composiciones necesarias para su puesta a punto y su uso en la elaboracion de vacunas frente al ibdv.
WO2005026375A2 (fr)	2003-05-22	2005-03-24	Fraunhofer Usa, Inc.	Molecule support recombinee pour l'expression, l'administration et la purification de polypeptides cibles
US8080255B2 (en)	2003-07-11	2011-12-20	Novavax Inc.	Functional influenza virus like particles (VLPs)
US8551756B2 (en) *	2003-07-11	2013-10-08	Novavax, Inc.	Avian influenza chimeric VLPS
US8592197B2 (en) *	2003-07-11	2013-11-26	Novavax, Inc.	Functional influenza virus-like particles (VLPs)
AU2011218606B2 (en) *	2003-07-11	2012-01-12	Novavax, Inc	Functional influenza virus-like particles (VLPS)
US8992939B2 (en)	2003-07-11	2015-03-31	Novavax, Inc.	Highly efficient influenza matrix (M1) proteins
US8506967B2 (en)	2003-07-11	2013-08-13	Novavax, Inc.	Functional influenza virus like particles (VLPs)
ES2307346B1 (es) *	2004-01-21	2009-11-13	Consejo Sup. Investig. Cientificas	Capsidas vacias (vlps(-vp4)) del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones.
ES2307345B1 (es) *	2004-01-21	2009-11-13	Consejo Sup. Investig. Cientificas	Capsidas vacias quimericas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones.
EP2848692B1 (fr) *	2004-05-21	2017-08-16	Novartis Vaccines and Diagnostics, Inc.	Vecteurs d'alphavirus de vaccins contre le virus de la grippe
AU2006214278C1 (en) *	2005-02-16	2012-07-19	Miltenyi Biotec Technology, Inc.	Lentiviral vectors and their use
ES2310062B1 (es)	2005-07-15	2009-11-13	Bionostra, S.L.	Particulas pseudovirales vacias quimericas derivadas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), procedimiento de obtencion y aplicaciones.
WO2007117264A2 (fr)	2005-08-03	2007-10-18	Fraunhofer Usa, Inc.	Compositions et procedes de production d'immunoglobulines
SI1937301T1 (sl) *	2005-10-18	2015-08-31	Novavax, Inc.	Funkcionalni influenčnemu virusu podobni delci (VLP)
KR101492643B1 (ko) *	2005-12-02	2015-02-12	이칸 스쿨 오브 메디슨 엣 마운트 시나이	비-천연 표면 단백질을 제시하는 키메라 바이러스 및 그의용도
ES2385842T3 (es) *	2005-12-06	2012-08-01	Yeda Research And Development Co., Ltd.	Vacuna mejorada contra la gripe
SG169375A1 (en) *	2006-02-02	2011-03-30	Globeimmune Inc	Yeast-based vaccine for inducing an immune response
CA2642056A1 (fr) *	2006-02-13	2007-08-23	Fraunhofer Usa, Inc.	Antigenes du papillomavirus humain, compositions de vaccin et methodes
EP1984405A4 (fr) *	2006-02-13	2010-06-30	Fraunhofer Usa Inc	Antigenes de la grippe, compositions de vaccins et procedes associees
WO2008048344A2 (fr) *	2006-02-13	2008-04-24	Fraunhofer Usa, Inc.	Antigènes de bacillus anthracis, compositions pour vaccins et procédés connexes
US20070224205A1 (en)	2006-03-07	2007-09-27	Powell Thomas J	Compositions that include hemagglutinin, methods of making and methods of use thereof
WO2007126788A2 (fr) *	2006-03-27	2007-11-08	Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services	Méthodes et compositions pour le traitement et la prévention d'une infection virale
US7682619B2 (en)	2006-04-06	2010-03-23	Cornell Research Foundation, Inc.	Canine influenza virus
US9101578B2 (en) *	2006-05-01	2015-08-11	Technovax, Inc.	Polyvalent influenza virus-like particle (VLP) compositions
WO2007130327A2 (fr) *	2006-05-01	2007-11-15	Technovax, Inc.	Compositions de particules de type viral (vlp) de la grippe
KR100702034B1 (ko) *	2006-05-12	2007-03-30	삼성전자주식회사	반도체 장치, 이 장치의 전원 노이즈 감소 방법 및 인쇄회로 기판
US8153115B2 (en)	2006-06-15	2012-04-10	Ben-Gurion University Of The Negev Research And Development Authority	Virus-like particles for treatment of viral infections
JP2009543554A (ja)	2006-07-14	2009-12-10	サノフィパスツールバイオロジクスカンパニー	ベクターウイルスタンパク質への外来免疫決定基の直接トランスポゾン媒介挿入による組換え型ウイルスワクチンの構築
CA2657955A1 (fr) *	2006-07-27	2008-08-07	Ligocyte Pharmaceuticals, Inc.	Particules de type virus chimerique
EP2044198A4 (fr) *	2006-07-27	2010-09-08	Ligocyte Pharmaceuticals Inc	Particules de type virus grippal chimérique
US20100239605A1 (en) *	2006-09-29	2010-09-23	Sanofi Pasteur Biologics Co.	Recombinant Rhinovirus Vectors
EP2069503B1 (fr) *	2006-11-15	2016-01-06	Folia Biotech Inc.	Vaccins contre la grippe a base du virus de la mosaique du papayer
AU2007330494B2 (en) *	2006-12-06	2014-03-13	Seqirus UK Limited	Vaccines including antigen from four strains of influenza virus
WO2008100609A1 (fr) *	2007-02-16	2008-08-21	The Salk Institute For Biological Studies	Nouvelle antitoxine et plateforme vaccinale à base de vlp de nodavirus
US8778348B2 (en) *	2007-04-28	2014-07-15	Ibio Inc.	Trypanosoma antigens, vaccine compositions, and related methods
WO2008148104A1 (fr)	2007-05-25	2008-12-04	Novavax, Inc.	Nouvelles vlp dérivées de cellules qui n'expriment pas une matrice virale ou une protéine du noyau
EP2178558B1 (fr)	2007-07-11	2014-04-30	iBio, Inc.	Antigènes yersinia pestis, compositions de vaccins, et méthodes associées
CN101795709B (zh)	2007-08-02	2013-07-17	彼昂德瓦克斯医药有限公司	多亚基多表位流感疫苗
US20110059130A1 (en) *	2007-08-20	2011-03-10	Fraunhofer Usa, Inc.	Prophylactic and therapeutic influenza vaccines, antigens, compositions and methods
EA034733B1 (ru) *	2008-01-21	2020-03-13	Медикаго Инк.	Нуклеиновая кислота для увеличенной экспрессии гемагглютинина вируса гриппа в растении и ее применение
SG190562A1 (en)	2008-04-18	2013-06-28	Vaxinnate Corp	Deletion mutants of flagellin and methods of use
WO2010012069A1 (fr) *	2008-07-30	2010-02-04	Folio Biotech Inc.	Vaccins multivalents à base du virus mosaïque de la papaye et leurs utilisations
US8734803B2 (en)	2008-09-28	2014-05-27	Ibio Inc.	Humanized neuraminidase antibody and methods of use thereof
PL2396032T3 (pl)	2009-02-10	2017-05-31	Seqirus UK Limited	Szczepionki przeciw grypie o obniżonych zawartościach skwalenu
AU2010243489A1 (en) *	2009-05-01	2011-12-22	Redbiotec Ag	Recombinant virus-like particles encoded by multi-gene vector
JP2012525830A (ja) *	2009-05-08	2012-10-25	グラクソスミスクラインバイオロジカルズソシエテアノニム	均質化を含む細胞培養からのウイルスの製造方法
PL222497B1 (pl) *	2009-06-26	2016-08-31	Inst Biochemii I Biofizyki Pan	Wektorowa cząsteczka wirusopodobna, sposób jej wytwarzania, kompozycja farmaceutyczna oraz zastosowanie wektorowej cząsteczki wirusopodobnej
US9278998B2 (en)	2009-07-02	2016-03-08	Massachusetts Institute Of Technology	Compositions and methods for diagnosing and/or treating influenza infection
EP2483307A1 (fr)	2009-09-29	2012-08-08	Fraunhofer USA, Inc.	Anticorps dirigés contre l'hémagglutinine du virus de la grippe, compositions, et procédés associés
WO2011046925A1 (fr) *	2009-10-12	2011-04-21	Technovax, Inc.	Particules de type virus (vlp) du virus respiratoire syncytial (vrs)
JP5917402B2 (ja)	2009-11-03	2016-05-11	タケダヴァクシーンズ，インコーポレイテッド	キメラＲＳＶ−Ｆポリペプチド、およびレンチウイルスＧａｇまたはアルファレトロウイルスＧａｇに基づくＶＬＰ
JP6073031B2 (ja) *	2009-12-28	2017-02-01	タケダヴァクシーンズ，インコーポレイテッド	インフルエンザ抗原のエンベロープウイルスベースのウイルス様粒子溶液を安定化させる方法
IN2012DN06277A (fr) *	2009-12-28	2015-09-25	Dsm Ip Assets Bv
US9352031B2 (en)	2010-02-18	2016-05-31	Technovax, Inc.	Universal virus-like particle (VLP) influenza vaccines
CN102373183A (zh) *	2010-08-09	2012-03-14	中山大学	禽流感和新城疫混合病毒样颗粒、制备方法和应用
CN102370976B (zh) *	2010-08-09	2014-06-11	中山大学	猪流感病毒和猪口蹄疫病毒的混合病毒样颗粒、制备方法和应用
CN102373182A (zh) *	2010-08-09	2012-03-14	中山大学	禽流感和传染性囊病混合病毒样颗粒、制备方法和应用
CN102373184A (zh) *	2010-08-09	2012-03-14	中山大学	禽流感和传染性支气管炎混合病毒样颗粒、制备方法和应用
CN102373181B (zh) *	2010-08-09	2014-06-11	中山大学	猪流感病毒和猪蓝耳病病毒混合病毒样颗粒、制备方法和应用
AU2011305371B2 (en)	2010-09-21	2015-05-21	Massachusetts Institute Of Technology	Human-adapted HA polypeptides, vaccines, and influenza treatment
US10226527B2 (en)	2010-10-04	2019-03-12	Massachusetts Institute Of Technology	Hemagglutinin polypeptides, and reagents and methods relating thereto
US9821051B1 (en)	2010-10-28	2017-11-21	Seqirus UK Limited	Reducing hospitalization in elderly influenza vaccine recipients
CA2828068C (fr)	2011-02-22	2019-03-19	Biondvax Pharmaceuticals Ltd.	Polypeptides multimeres multi-epitopes utilises dans des vaccins contre la grippe saisonniere et pandemique
CN106995488A (zh)	2011-06-20	2017-08-01	高等教育联邦系统-匹兹堡大学	计算优化的宽反应性的h1n1流感抗原
EP3275464A1 (fr) *	2011-08-01	2018-01-31	Emory University	Vlps contenant des ligands et procédés associés
SG11201400743VA (en) *	2011-09-20	2014-04-28	Sinai School Medicine	Influenza virus vaccines and uses thereof
US9364530B2 (en)	2012-03-22	2016-06-14	Fraunhofer Usa, Inc.	Virus-like particles comprising a matrix protein from a plant enveloped virus and uses thereof
SG11201406153XA (en)	2012-03-30	2014-10-30	Univ Pittsburgh	Computationally optimized broadly reactive antigens for h5n1 and h1n1 influenza viruses
TWI434933B (zh) *	2012-04-17	2014-04-21	Nat Univ Tsing Hua	抗多型禽流感病毒之ｄｎａ疫苗及其組合物
US8932598B2 (en)	2012-08-28	2015-01-13	Vaxinnate Corporation	Fusion proteins and methods of use
US9309290B2 (en)	2012-11-27	2016-04-12	University of Pittsburgh—of the Commonwealth System of Higher Education	Computationally optimized broadly reactive antigens for H1N1 influenza
US9371366B2 (en)	2012-12-18	2016-06-21	Icahn School Of Medicine At Mount Sinai	Influenza virus vaccines and uses thereof
WO2014159960A1 (fr)	2013-03-14	2014-10-02	Icahn School Of Medicine At Mount Sinai	Anticorps contre l'hémagglutinine du virus de la grippe et utilisations correspondantes
US10080792B2 (en)	2013-09-23	2018-09-25	Engen Bio, Inc.	Influenza vaccine and therapy
CN104436157A (zh)	2013-09-23	2015-03-25	恩金生物有限公司	流感疫苗和治疗
WO2016118937A1 (fr)	2015-01-23	2016-07-28	Icahn School Of Medicine At Mount Sinai	Régimes de vaccination contre le virus de la grippe
KR101729387B1 (ko)	2015-04-17	2017-04-21	경희대학교 산학협력단	곤충세포 발현시스템을 이용한 인플루엔자 백신 소재 ｍ1-ｍ2-ｎａ-ｈａ 바이러스 유사 입자의 제조방법
CN114617959A (zh)	2015-09-03	2022-06-14	诺瓦瓦克斯股份有限公司	具有改进的稳定性和免疫原性的疫苗组合物
WO2017044890A1 (fr) *	2015-09-10	2017-03-16	Academia Sinica	Combinaison de vaccins contre la grippe aviaire comprenant des particules analogues à un virus et de nouveaux adjuvants
CN109641041A (zh)	2016-06-15	2019-04-16	西奈山伊坎医学院	流感病毒血细胞凝集素蛋白及其用途
CN110325202B (zh) *	2016-10-31	2023-10-31	佐治亚州立大学研究基金会公司	乙型肝炎病毒pre-S蛋白的病毒样颗粒
CA3058652A1 (fr)	2017-04-07	2018-10-11	Icahn School Of Medicine At Mount Sinai	Anticorps anti-neuraminidase du virus influenza de type b et leurs utilisations
EP3851120A4 (fr)	2018-09-11	2022-04-27	Shanghai Public Health Clinical Center	Immunogène pour vaccin à large spectre contre la grippe, et application de celui-ci
MX2023012554A (es)	2018-12-12	2023-11-08	Cambridge Tech Llc	Vacuna universal contra la gripe.
CN116063409A (zh) *	2022-09-09	2023-05-05	中山大学·深圳	一种乙型流感Mosaic重组蛋白、重组质粒及其构建方法和应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4009258A (en) *	1972-09-25	1977-02-22	The Mount Sinai School Of Medicine Of The City University Of New York	Influenza vaccine containing a recombinant, antigenically hybridized virus and method of using the same
US4666829A (en)	1985-05-15	1987-05-19	University Of California	Polypeptide marker for Alzheimer's disease and its use for diagnosis
US6001634A (en) *	1989-08-28	1999-12-14	Palese; Peter	Recombinant negative strand RNA viruses
US5298244A (en)	1990-10-25	1994-03-29	University Of Saskatchewan	Assembled viral particles and their use in a vaccine to rotaviral disease
US5830877A (en)	1993-08-26	1998-11-03	The Regents Of The University Of California	Method, compositions and devices for administration of naked polynucleotides which encode antigens and immunostimulatory
US6207646B1 (en)	1994-07-15	2001-03-27	University Of Iowa Research Foundation	Immunostimulatory nucleic acid molecules
TWI239847B (en)	1997-12-02	2005-09-21	Elan Pharm Inc	N-terminal fragment of Abeta peptide and an adjuvant for preventing and treating amyloidogenic disease
DE69935507T2 (de)	1998-04-03	2007-12-06	University Of Iowa Research Foundation	Verfahren und produkte zur stimulierung des immunsystems mittels immunotherapeutischer oligonukleotide und zytokine
JP4673974B2 (ja)	1998-09-30	2011-04-20	ワイス・ホールディングズ・コーポレイション	アジュバントとしての変異コレラホロトキシン
DE122008000061I1 (de) *	1999-04-06	2009-02-12	Wisconsin Alumni Res Found	Rekombinante influenzaviren zur vakzinherstellung und gentherapie
AU2001268662A1 (en) *	2000-06-23	2002-01-08	Wyeth Holdings Corporation	Assembly of wild-type and chimeric influenza virus-like particles (VLPs)

2001
- 2001-06-21 AU AU2001268662A patent/AU2001268662A1/en not_active Abandoned
- 2001-06-21 WO PCT/US2001/019890 patent/WO2002000885A2/fr active IP Right Grant
- 2001-06-21 CA CA2410297A patent/CA2410297C/fr not_active Expired - Lifetime
- 2001-06-21 JP JP2002506200A patent/JP5008244B2/ja not_active Expired - Lifetime
- 2001-06-21 EP EP01946643A patent/EP1294892B1/fr not_active Expired - Lifetime
- 2001-06-21 PT PT01946643T patent/PT1294892E/pt unknown
- 2001-06-21 ES ES01946643T patent/ES2295175T3/es not_active Expired - Lifetime
- 2001-06-21 BR BRPI0111830A patent/BRPI0111830B8/pt not_active IP Right Cessation
- 2001-06-21 KR KR1020027017581A patent/KR100877019B1/ko not_active Expired - Lifetime
- 2001-06-21 DK DK01946643T patent/DK1294892T3/da active
- 2001-06-21 CN CNB018116434A patent/CN1196788C/zh not_active Expired - Lifetime
- 2001-06-21 AT AT01946643T patent/ATE376059T1/de active
- 2001-06-21 IL IL15329001A patent/IL153290A0/xx unknown
- 2001-06-21 DE DE60130987T patent/DE60130987T2/de not_active Expired - Lifetime
- 2001-06-21 MX MXPA02012254A patent/MXPA02012254A/es active IP Right Grant
2005
- 2005-03-28 US US11/091,309 patent/US7556940B2/en not_active Expired - Lifetime
2007
- 2007-04-18 AU AU2007201727A patent/AU2007201727B2/en not_active Expired
- 2007-12-17 CY CY20071101598T patent/CY1107835T1/el unknown
2009
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Publication number	Publication date
WO2002000885A3 (fr)	2002-08-08
DE60130987T2 (de)	2008-07-24
US8859231B2 (en)	2014-10-14
ATE376059T1 (de)	2007-11-15
BR0111830A (pt)	2003-03-25
CA2410297C (fr)	2011-03-15
IL153290A0 (en)	2003-07-06
DE60130987D1 (de)	2007-11-29
EP1294892A2 (fr)	2003-03-26
US7556940B2 (en)	2009-07-07
WO2002000885A9 (fr)	2002-05-10
BR0111830B1 (pt)	2014-12-02
CY1107835T1 (el)	2013-06-19
AU2007201727B2 (en)	2009-06-04
WO2002000885A2 (fr)	2002-01-03
US20090239261A1 (en)	2009-09-24
CN1437655A (zh)	2003-08-20
KR100877019B1 (ko)	2009-01-07
EP1294892B1 (fr)	2007-10-17
CA2410297A1 (fr)	2002-01-03
AU2007201727A1 (en)	2007-05-10
US20050186621A1 (en)	2005-08-25
ES2295175T3 (es)	2008-04-16
DK1294892T3 (da)	2008-01-28
BRPI0111830B8 (pt)	2021-05-25
MXPA02012254A (es)	2003-04-25
JP5008244B2 (ja)	2012-08-22
PT1294892E (pt)	2008-01-16
KR20030013463A (ko)	2003-02-14
JP2004501647A (ja)	2004-01-22
CN1196788C (zh)	2005-04-13

Publication	Publication Date	Title
US8859231B2 (en)	2014-10-14	Assembly of wild-type and chimeric influenza virus-like particles (VLPs)
US9956280B2 (en)	2018-05-01	Functional influenza virus-like particles (VLPs)
EP1862550A1 (fr)	2007-12-05	Ensemble de particules de virus du type de la grippe chimérique et sauvage (VLP)
AU2011218606B2 (en)	2012-01-12	Functional influenza virus-like particles (VLPS)

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