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AU2001244921A1 - Method of concentrating and recovering a viral enzyme activity from biological samples - Google Patents

Method of concentrating and recovering a viral enzyme activity from biological samples

Info

Publication number
AU2001244921A1
AU2001244921A1 AU2001244921A AU4492101A AU2001244921A1 AU 2001244921 A1 AU2001244921 A1 AU 2001244921A1 AU 2001244921 A AU2001244921 A AU 2001244921A AU 4492101 A AU4492101 A AU 4492101A AU 2001244921 A1 AU2001244921 A1 AU 2001244921A1
Authority
AU
Australia
Prior art keywords
enzyme activity
recovering
buffer solution
matrix
concentrating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001244921A
Inventor
Tommy Gatu
Simon Gronowitz
Clas Kallander
Anders Malmsten
Ingvar Pettersson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cavidi Tech AB
Original Assignee
Cavidi Tech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cavidi Tech AB filed Critical Cavidi Tech AB
Publication of AU2001244921A1 publication Critical patent/AU2001244921A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method of concentrating and recovering an enzyme activity from enveloped viruses present in a biological sample, is described. The method comprises contacting the biological sample in a first buffer solution with a virus-binding matrix, such as an anion exchanger matrix, to attach virus particles present in the sample to the matrix, washing the matrix carrying the virus particles with a second buffer solution to remove components interfering with viral enzyme activity, lysing the immobilized virus particles in a third buffer solution and recovering the concentrated viral enzyme activity from the third buffer solution. Additionally, a commercial package containing written and/or data carrier instructions for performing laboratory steps for concentration and recovery of an enzyme activity from enveloped viruses present in a biological sample and at least one component necessary for the assay, is disclosed.
AU2001244921A 2000-03-29 2001-03-22 Method of concentrating and recovering a viral enzyme activity from biological samples Abandoned AU2001244921A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0001132A SE0001132D0 (en) 2000-03-29 2000-03-29 Method of concentrating and recovering a viral enzyme activity from biological samples
SE0001132 2000-03-29
PCT/SE2001/000617 WO2001075147A1 (en) 2000-03-29 2001-03-22 Method of concentrating and recovering a viral enzyme activity from biological samples

Publications (1)

Publication Number Publication Date
AU2001244921A1 true AU2001244921A1 (en) 2001-10-15

Family

ID=20279073

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001244921A Abandoned AU2001244921A1 (en) 2000-03-29 2001-03-22 Method of concentrating and recovering a viral enzyme activity from biological samples

Country Status (12)

Country Link
US (1) US7833700B2 (en)
EP (1) EP1268851B1 (en)
CN (1) CN1204265C (en)
AT (1) ATE307897T1 (en)
AU (1) AU2001244921A1 (en)
BR (1) BRPI0109674B8 (en)
DE (1) DE60114389T2 (en)
DK (1) DK1268851T3 (en)
ES (1) ES2252206T3 (en)
HK (1) HK1053855B (en)
SE (1) SE0001132D0 (en)
WO (1) WO2001075147A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0101219D0 (en) 2001-04-05 2001-04-05 Cavidi Tech Ab Recovery of enzyme activity from enveloped viruses
AU2002309447B2 (en) * 2001-06-14 2007-05-24 Cavidi Tech Ab Viral drug susceptibility testing
DE10230819B4 (en) * 2002-07-08 2007-12-06 Kronotec Ag floor panel
WO2004042003A2 (en) 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5482834A (en) * 1982-05-17 1996-01-09 Hahnemann University Evaluation of nucleic acids in a biological sample hybridization in a solution of chaotrophic salt solubilized cells
US4707439A (en) * 1984-10-26 1987-11-17 The United States Of America As Represented By The Department Of Health And Human Services Screening test for reverse-transcriptase containing virus such as non-A, non-B hepatitis, NANBH
EP0587832B1 (en) * 1992-02-10 2001-07-11 Baxter International Inc. Method for testing blood units for viral contamination
US5658779A (en) 1992-03-20 1997-08-19 Ligochem, Inc. Method of adsorbing viruses from fluid compositions
AU2127395A (en) * 1994-03-22 1995-10-09 Rhone-Poulenc Rorer Pharmaceuticals Inc. Highly efficient production and isolation of viral particles
SE9902410D0 (en) * 1999-06-24 1999-06-24 Cavidi Tech Ab Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples

Also Published As

Publication number Publication date
ATE307897T1 (en) 2005-11-15
BRPI0109674A (en) 2003-02-04
CN1204265C (en) 2005-06-01
BRPI0109674B1 (en) 2014-02-11
EP1268851B1 (en) 2005-10-26
SE0001132D0 (en) 2000-03-29
WO2001075147A1 (en) 2001-10-11
BRPI0109674B8 (en) 2021-07-06
DE60114389D1 (en) 2005-12-01
CN1419605A (en) 2003-05-21
EP1268851A1 (en) 2003-01-02
HK1053855A1 (en) 2003-11-07
HK1053855B (en) 2006-09-01
ES2252206T3 (en) 2006-05-16
DE60114389T2 (en) 2006-07-13
DK1268851T3 (en) 2006-03-13
US7833700B2 (en) 2010-11-16
US20030104441A1 (en) 2003-06-05

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