AU2000258017A1 - Human source gene leading sequence, gene vector and gene expression strategy - Google Patents
Human source gene leading sequence, gene vector and gene expression strategyInfo
- Publication number
- AU2000258017A1 AU2000258017A1 AU2000258017A AU5801700A AU2000258017A1 AU 2000258017 A1 AU2000258017 A1 AU 2000258017A1 AU 2000258017 A AU2000258017 A AU 2000258017A AU 5801700 A AU5801700 A AU 5801700A AU 2000258017 A1 AU2000258017 A1 AU 2000258017A1
- Authority
- AU
- Australia
- Prior art keywords
- gene
- human
- sequence
- leading sequence
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 11
- 230000014509 gene expression Effects 0.000 title abstract 4
- 210000000349 chromosome Anatomy 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000010276 construction Methods 0.000 abstract 1
- 238000010363 gene targeting Methods 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 210000005260 human cell Anatomy 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000035790 physiological processes and functions Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Electrotherapy Devices (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The p resent i nvention d eals w ith the leading sequence o f human source gene, gene vector and gene expression strategies. The invention include the following: (1) Using DNA sequence without pivot physiological function-related gene in short arms of human group D, G chromosomes, or a DNA sequence sharing 50% or over 50% identity to the sequence select from human D, G group chromosomes as leading sequence for gene targeting; (2) Construct the gene vector containing the leading sequence above; (3) Nucleolus organizing region in D, G group chromosomes mentioned in the procedure(1) is used as the target site, the gene of interest is integrated into the short arms where the gene expresses actively in D,G group chromosomes of human cells. The present invention provides a novel gene leading sequence by which the gene vector construction and gene expression strategies are obtained. The gene expression strategies can be used for human gene therapy and manufacturing protein.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2000/000203 WO2002010375A1 (en) | 2000-07-17 | 2000-07-17 | Human source gene leading sequence, gene vector and gene expression strategy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2000258017A1 true AU2000258017A1 (en) | 2002-02-13 |
Family
ID=4574675
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2000258017A Abandoned AU2000258017A1 (en) | 2000-07-17 | 2000-07-17 | Human source gene leading sequence, gene vector and gene expression strategy |
| AU2001237201A Abandoned AU2001237201A1 (en) | 2000-07-17 | 2001-02-16 | Human source gene leading sequence, gene vector and gene expression strategy |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001237201A Abandoned AU2001237201A1 (en) | 2000-07-17 | 2001-02-16 | Human source gene leading sequence, gene vector and gene expression strategy |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7247431B2 (en) |
| EP (1) | EP1304380B1 (en) |
| JP (1) | JP4944349B2 (en) |
| CN (1) | CN1287861C (en) |
| AT (1) | ATE466940T1 (en) |
| AU (2) | AU2000258017A1 (en) |
| DE (1) | DE60142052D1 (en) |
| WO (2) | WO2002010375A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2000268170A1 (en) * | 2000-09-04 | 2002-03-22 | Hunan Royal Biotech | A cell line expressing mutant human tissue-type plasminogen activator, constructing strategy and method of expressed protein preparation |
| CN114958901B (en) * | 2022-05-24 | 2024-06-04 | 华中科技大学 | In vitro preparation, activity detection and application of an antifungal drug target |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5670314A (en) | 1994-02-22 | 1997-09-23 | Regents Of The University Of California | Genetic alterations that correlate with lung carcinomas |
| GB9410446D0 (en) * | 1994-05-25 | 1994-07-13 | Cancer Res Campaign Tech | Novel chromosome fragment |
| US5773289A (en) * | 1995-06-06 | 1998-06-30 | University Of Pittsburgh | AAV directed targeted integration |
| US5843757A (en) * | 1995-08-24 | 1998-12-01 | The Johns Hopkins University | Human JTV1 gene overlaps PMS2 gene |
| US6025155A (en) * | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| IT1291135B1 (en) * | 1997-04-08 | 1998-12-29 | Angeletti P Ist Richerche Bio | RECOMBINANT VECTORS USABLE IN GENE THERAPY |
| WO1999005295A1 (en) * | 1997-07-25 | 1999-02-04 | Thomas Jefferson University | Composition and method for targeted integration into cells |
-
2000
- 2000-07-17 AU AU2000258017A patent/AU2000258017A1/en not_active Abandoned
- 2000-07-17 WO PCT/CN2000/000203 patent/WO2002010375A1/en active Application Filing
-
2001
- 2001-02-16 AT AT01909410T patent/ATE466940T1/en not_active IP Right Cessation
- 2001-02-16 US US10/333,069 patent/US7247431B2/en not_active Expired - Lifetime
- 2001-02-16 EP EP01909410A patent/EP1304380B1/en not_active Expired - Lifetime
- 2001-02-16 DE DE60142052T patent/DE60142052D1/en not_active Expired - Lifetime
- 2001-02-16 AU AU2001237201A patent/AU2001237201A1/en not_active Abandoned
- 2001-02-16 CN CNB018128734A patent/CN1287861C/en not_active Expired - Fee Related
- 2001-02-16 JP JP2002525810A patent/JP4944349B2/en not_active Expired - Fee Related
- 2001-02-16 WO PCT/CN2001/000126 patent/WO2002020803A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| US7247431B2 (en) | 2007-07-24 |
| WO2002010375A1 (en) | 2002-02-07 |
| EP1304380A1 (en) | 2003-04-23 |
| EP1304380A4 (en) | 2005-06-22 |
| EP1304380B1 (en) | 2010-05-05 |
| ATE466940T1 (en) | 2010-05-15 |
| AU2001237201A1 (en) | 2002-03-22 |
| WO2002020803A1 (en) | 2002-03-14 |
| DE60142052D1 (en) | 2010-06-17 |
| CN1287861C (en) | 2006-12-06 |
| JP4944349B2 (en) | 2012-05-30 |
| JP2004508046A (en) | 2004-03-18 |
| US20040137437A1 (en) | 2004-07-15 |
| CN1471581A (en) | 2004-01-28 |
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