ATE536408T1 - MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE - Google Patents
MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCEInfo
- Publication number
- ATE536408T1 ATE536408T1 AT04749718T AT04749718T ATE536408T1 AT E536408 T1 ATE536408 T1 AT E536408T1 AT 04749718 T AT04749718 T AT 04749718T AT 04749718 T AT04749718 T AT 04749718T AT E536408 T1 ATE536408 T1 AT E536408T1
- Authority
- AT
- Austria
- Prior art keywords
- rna interference
- terminal
- modifications
- modified polynucleotides
- nucleotides
- Prior art date
Links
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 title abstract 2
- 230000009368 gene silencing by RNA Effects 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 title 1
- 230000004048 modification Effects 0.000 abstract 4
- 238000012986 modification Methods 0.000 abstract 4
- 108020004459 Small interfering RNA Proteins 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 230000000692 anti-sense effect Effects 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 230000030279 gene silencing Effects 0.000 abstract 1
- 238000012226 gene silencing method Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000009437 off-target effect Effects 0.000 abstract 1
- 230000026731 phosphorylation Effects 0.000 abstract 1
- 238000006366 phosphorylation reaction Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
<P>PROBLEM TO BE SOLVED: To provide a modification for an siRNA capable of increasing or decreasing specificity and/or stability. <P>SOLUTION: There are provided a method and a composition for performing RNA interference comprising a wide variety of stabilized siRNAs suitable for use in serum-containing media and for in vivo applications (such as therapeutic applications). Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications include phosphorylation of the first 5' terminal antisense nucleotide; 2' carbon modifications of the first and second or first, second and third 5' terminal antisense nucleotides; and optionally 2' carbon modifications of the first and second or first, second and third 5' terminal sense nucleotides. Control and exaequo molecules are also provided. <P>COPYRIGHT: (C)2010,JPO&INPIT
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/406,908 US20040198640A1 (en) | 2003-04-02 | 2003-04-02 | Stabilized polynucleotides for use in RNA interference |
| US10/613,077 US20040266707A1 (en) | 2003-04-02 | 2003-07-01 | Stabilized polynucleotides for use in RNA interference |
| US54266804P | 2004-02-06 | 2004-02-06 | |
| US54264604P | 2004-02-06 | 2004-02-06 | |
| US54364004P | 2004-02-10 | 2004-02-10 | |
| US54366104P | 2004-02-10 | 2004-02-10 | |
| PCT/US2004/010343 WO2004090105A2 (en) | 2003-04-02 | 2004-04-01 | Modified polynucleotides for use in rna interference |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE536408T1 true ATE536408T1 (en) | 2011-12-15 |
Family
ID=42703870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04749718T ATE536408T1 (en) | 2003-04-02 | 2004-04-01 | MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP5468978B2 (en) |
| AT (1) | ATE536408T1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR083445A1 (en) | 2010-10-14 | 2013-02-27 | Univ Mie | siRNA AGAINST FIBROSIS |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL155991A0 (en) * | 2000-12-01 | 2003-12-23 | Max Planck Gesellschaft | Rna interference mediating small rna molecules |
| WO2003012052A2 (en) * | 2001-07-30 | 2003-02-13 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Specific inhibition of gene expression by small double stranded rnas |
| BRPI0313202A8 (en) * | 2002-08-05 | 2016-08-16 | Atugen Ag | ADDITIONAL WAYS TO INTERFERE WITH RNA MOLECULES |
-
2004
- 2004-04-01 AT AT04749718T patent/ATE536408T1/en active
-
2010
- 2010-04-30 JP JP2010106021A patent/JP5468978B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP5468978B2 (en) | 2014-04-09 |
| JP2010172344A (en) | 2010-08-12 |
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