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ATE517188T1 - Expressions-cloning-verfahren zur auswahl von bibliotheksklonen zur herstellung eines polypeptids von interesse - Google Patents

Expressions-cloning-verfahren zur auswahl von bibliotheksklonen zur herstellung eines polypeptids von interesse

Info

Publication number
ATE517188T1
ATE517188T1 AT08750156T AT08750156T ATE517188T1 AT E517188 T1 ATE517188 T1 AT E517188T1 AT 08750156 T AT08750156 T AT 08750156T AT 08750156 T AT08750156 T AT 08750156T AT E517188 T1 ATE517188 T1 AT E517188T1
Authority
AT
Austria
Prior art keywords
interest
library
polynucleotide
polypeptide
host cell
Prior art date
Application number
AT08750156T
Other languages
English (en)
Inventor
Jesper Vind
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE517188T1 publication Critical patent/ATE517188T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT08750156T 2007-05-09 2008-05-07 Expressions-cloning-verfahren zur auswahl von bibliotheksklonen zur herstellung eines polypeptids von interesse ATE517188T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700694 2007-05-09
PCT/EP2008/055638 WO2008138835A1 (en) 2007-05-09 2008-05-07 Expression cloning method suitable for selecting library clones producing a polypeptide of interest

Publications (1)

Publication Number Publication Date
ATE517188T1 true ATE517188T1 (de) 2011-08-15

Family

ID=38226473

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08750156T ATE517188T1 (de) 2007-05-09 2008-05-07 Expressions-cloning-verfahren zur auswahl von bibliotheksklonen zur herstellung eines polypeptids von interesse

Country Status (7)

Country Link
US (1) US20100221783A1 (de)
EP (1) EP2147107B1 (de)
JP (1) JP5497632B2 (de)
CN (1) CN101679993A (de)
AT (1) ATE517188T1 (de)
DK (1) DK2147107T3 (de)
WO (1) WO2008138835A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2456872B1 (de) 2009-07-22 2017-08-30 DSM IP Assets B.V. Verbesserte wirtszelle für die herstellung von relevanten produkten
DK2683732T3 (en) * 2011-03-11 2016-12-12 Dsm Ip Assets Bv Vector-host-system
EP3597736A1 (de) 2011-11-21 2020-01-22 Novozymes A/S Gh61-polypepvarianten und dafür codierende polynukleotide
US10227579B2 (en) 2012-04-27 2019-03-12 Novozymes A/S GH61 polypeptide variants and polynucleotides encoding same
CN104837993A (zh) * 2012-12-07 2015-08-12 诺维信公司 用于在丝状真菌中产生位点特异性突变的方法
WO2015059133A1 (en) 2013-10-22 2015-04-30 Novozymes A/S Cellobiose dehydrogenase variants and polynucleotides encoding same
US20160304905A1 (en) * 2013-12-03 2016-10-20 Novozymes A/S Fungal Gene Library By Double Split-Marker Integration
US10123535B2 (en) 2014-03-05 2018-11-13 Novozymes A/S Compositions and methods for improving post-harvest properties of agricultural crops
AR099683A1 (es) 2014-03-05 2016-08-10 Novozymes Bioag As Composiciones que comprenden polímeros de xiloglucano como portador para agentes agrícolamente benéficos
CN106062270A (zh) 2014-03-05 2016-10-26 诺维信公司 使用木葡聚糖内糖基转移酶改进非纤维素纺织材料的性质的组合物和方法
CN106062276B (zh) 2014-03-05 2019-06-11 诺维信公司 用于将材料功能化并且进行连接的组合物和方法
US20160333292A1 (en) 2014-03-05 2016-11-17 Novozymes A/S Compositions and Methods for Improving Properties of Cellulosic Textile Materials with Xyloglucan Endotransglycosylase
CN107223157B (zh) * 2014-12-16 2021-01-08 丹尼斯科美国公司 用于辅助菌株介导的真菌基因组修饰的组合物和方法
CN104894115B (zh) * 2015-05-26 2018-11-13 中国科学院青岛生物能源与过程研究所 一种具有高效同源重组能力的土曲霉及其构建方法与应用
CN104894165B (zh) * 2015-05-26 2019-01-25 中国科学院青岛生物能源与过程研究所 一种提高基因打靶技术在土曲霉中应用效率的方法与应用
WO2017070219A1 (en) 2015-10-20 2017-04-27 Novozymes A/S Lytic polysaccharide monooxygenase (lpmo) variants and polynucleotides encoding same
US20190225988A1 (en) 2016-09-15 2019-07-25 Novozymes A/S Genomic integration of DNA fragments in fungal host cells
JP7181865B2 (ja) * 2017-06-23 2022-12-01 株式会社カネカ メタノール資化性酵母内において高効率にベクターをアセンブルする方法
WO2019227363A1 (zh) * 2018-05-31 2019-12-05 大连民族大学 一种地生弯颈霉发酵生产脂肪酶的培养基及其培养方法
WO2020173817A1 (en) 2019-02-28 2020-09-03 Novozymes A/S Calcite binding proteins
CN112301047A (zh) * 2020-11-02 2021-02-02 江苏东玄基因科技有限公司 一种精确调控重组蛋白表达的方法

Family Cites Families (16)

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Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
JP2703598B2 (ja) 1987-09-04 1998-01-26 ノボ―ノルディスク アクティーゼルスカブ アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター
CA1341226C (en) 1988-08-16 2001-05-01 Wim Van Hartingsveldt Gene replacement as a tool for the construction of aspergillus strains
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
PT765394E (pt) 1994-06-03 2002-03-28 Novozymes Biotech Inc Lacases myceliophthora purificadas e acidos nucleicos que as codificam
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
JP3792725B2 (ja) 1996-01-19 2006-07-05 ノボザイムス，インコーポレイティド 糸状菌の形態変異体
DK1616580T3 (da) * 1996-07-05 2008-01-14 Novozymes As Transkriptionsfaktor
JP5043254B2 (ja) 1998-10-26 2012-10-10 ノボザイムス アクティーゼルスカブ 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング
WO2001051646A2 (en) 2000-01-13 2001-07-19 Novozymes Biotech, Inc. Methods for producing a polypeptide using a crippled translational initiator sequence
US6569681B1 (en) * 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
DK1266011T3 (da) 2000-03-14 2010-07-26 Novozymes As Svampetranskriptionsaktivator, der er anvendelig i fremgangsmåder til fremstilling af polypeptider
EP1217074A1 (de) * 2000-12-22 2002-06-26 Universiteit Leiden Integration von Nukleinsäure in Eukaryoten
US7063962B2 (en) * 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
ATE432355T1 (de) 2002-02-19 2009-06-15 Novozymes As Expressionsklonierungsverfahren in filamentösen pilzen
DE602005023427D1 (de) * 2004-04-02 2010-10-21 Dsm Ip Assets Bv Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz

Also Published As

Publication number Publication date
JP5497632B2 (ja) 2014-05-21
EP2147107B1 (de) 2011-07-20
US20100221783A1 (en) 2010-09-02
JP2010525830A (ja) 2010-07-29
WO2008138835A1 (en) 2008-11-20
EP2147107A1 (de) 2010-01-27
DK2147107T3 (da) 2011-10-24
CN101679993A (zh) 2010-03-24

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