[go: up one dir, main page]

ATE505540T1 - Genabschaltung (gene silencing) durch hybride sens-dna und antisens-rna konstrukte, gekoppelt an ein peptid zur erleichterten aufnahme in zellen - Google Patents

Genabschaltung (gene silencing) durch hybride sens-dna und antisens-rna konstrukte, gekoppelt an ein peptid zur erleichterten aufnahme in zellen

Info

Publication number
ATE505540T1
ATE505540T1 AT04027185T AT04027185T ATE505540T1 AT E505540 T1 ATE505540 T1 AT E505540T1 AT 04027185 T AT04027185 T AT 04027185T AT 04027185 T AT04027185 T AT 04027185T AT E505540 T1 ATE505540 T1 AT E505540T1
Authority
AT
Austria
Prior art keywords
peptide
hybrid
cells
coupled
gene silencing
Prior art date
Application number
AT04027185T
Other languages
English (en)
Inventor
Martin Weber
Ioanna Andreou
Nicole Bezay
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE505540T1 publication Critical patent/ATE505540T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT04027185T 2004-11-16 2004-11-16 Genabschaltung (gene silencing) durch hybride sens-dna und antisens-rna konstrukte, gekoppelt an ein peptid zur erleichterten aufnahme in zellen ATE505540T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04027185A EP1657306B1 (de) 2004-11-16 2004-11-16 Genabschaltung (Gene silencing) durch hybride Sens-DNA und Antisens-RNA Konstrukte, gekoppelt an ein Peptid zur erleichterten Aufnahme in Zellen

Publications (1)

Publication Number Publication Date
ATE505540T1 true ATE505540T1 (de) 2011-04-15

Family

ID=34927409

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04027185T ATE505540T1 (de) 2004-11-16 2004-11-16 Genabschaltung (gene silencing) durch hybride sens-dna und antisens-rna konstrukte, gekoppelt an ein peptid zur erleichterten aufnahme in zellen

Country Status (5)

Country Link
US (1) US20090317906A1 (de)
EP (1) EP1657306B1 (de)
AT (1) ATE505540T1 (de)
DE (1) DE602004032245D1 (de)
WO (1) WO2006053683A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3342415B1 (de) 2006-08-08 2022-01-26 Rheinische Friedrich-Wilhelms-Universität Bonn Struktur und verwendung von 5´-phosphat-oligonukleotiden
TW200932274A (en) 2007-12-18 2009-08-01 Alcon Res Ltd Interfering RNA delivery system and uses thereof
JP5689413B2 (ja) 2008-05-21 2015-03-25 ライニッシュ フリードリッヒ−ウィルヘルムズ−ユニバーシタット ボン 平滑末端を有する５’三リン酸オリゴヌクレオチドおよびその使用
US20100190691A1 (en) * 2009-01-27 2010-07-29 Trojan Technologies, Ltd Delivery of nucleic acids using cell-penetrating peptides
EP4089169A1 (de) 2009-10-12 2022-11-16 Larry J. Smith Verfahren und zusammensetzungen zum modulieren der genexpression mit in-vivo oder in-vitro verabreichten arzneistoffen auf oligonukleotidbasis
WO2011127210A1 (en) * 2010-04-06 2011-10-13 Massachusetts Institute Of Technology Targeted delivery of nucleic acids
GB2480296A (en) * 2010-05-12 2011-11-16 Nds Ltd Processor with differential power analysis attack protection
US9540634B2 (en) 2010-06-07 2017-01-10 The Trustees Of The University Of Pennsylvania Transcriptome in vivo analysis
US9540680B2 (en) 2010-06-07 2017-01-10 The Trustees Of The University Of Pennsylvania Transcriptome in vivo analysis
EP2594638B1 (de) * 2010-07-10 2016-05-25 Kinki University Verfahren zur einführung von nukleinsäure in zellen und nukleinsäure-komplex
WO2012012518A2 (en) * 2010-07-20 2012-01-26 University Of Miami Inhibition of nonsense mediated decay pathways
EP2508530A1 (de) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Reinigung von triphosphorylierten Oligonucleotiden mit Einfang-Tags
WO2012159164A1 (en) * 2011-05-23 2012-11-29 Phylogica Limited Method of determining, identifying or isolating cell-penetrating peptides
EP2712870A1 (de) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Neuartige RIG-I-Liganden und Herstellungsverfahren dafür
KR101660016B1 (ko) * 2014-06-30 2016-09-30 서울시립대학교 산학협력단 Ｄｎａ―ｒｎａ 혼성 입자 및 그 제조방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US5986084A (en) 1997-08-18 1999-11-16 Pitsch; Stefan Ribonucleoside-derivative and method for preparing the same
DE10027777A1 (de) * 2000-06-07 2001-12-13 Otogene Ag Transfektionsverfahren
AU2002249967A1 (en) * 2001-11-12 2003-05-26 Epiclone, Inc. Gene silencing using sense dna and antisense rna hybrid constructs
KR20100087400A (ko) * 2001-11-21 2010-08-04 가오루 사이고 유전자 발현 억제 방법
WO2004007721A1 (en) * 2002-07-17 2004-01-22 University Of Otago Conjugate for double-stranded rna sequence releasing and methods thereof
WO2004041194A2 (en) * 2002-11-01 2004-05-21 University Of Medicine And Dentistry Of New Jersey Inhibition of hiv-1 replication
ATE465255T1 (de) * 2002-11-26 2010-05-15 Univ Massachusetts Verabreichung von sirnas
US20040147027A1 (en) * 2003-01-28 2004-07-29 Troy Carol M. Complex for facilitating delivery of dsRNA into a cell and uses thereof
US20050164212A1 (en) * 2003-03-06 2005-07-28 Todd Hauser Modulation of gene expression using DNA-RNA hybrids

Also Published As

Publication number Publication date
US20090317906A1 (en) 2009-12-24
DE602004032245D1 (de) 2011-05-26
WO2006053683A2 (en) 2006-05-26
EP1657306B1 (de) 2011-04-13
WO2006053683A3 (en) 2006-08-03
EP1657306A1 (de) 2006-05-17

Similar Documents

Publication Publication Date Title
ATE505540T1 (de) Genabschaltung (gene silencing) durch hybride sens-dna und antisens-rna konstrukte, gekoppelt an ein peptid zur erleichterten aufnahme in zellen
ATE500337T1 (de) Verfahren zur durchführung homologer rekombinationen in pflanzen
WO2007014275A3 (en) Targeted integration and expression of exogenous nucleic acid sequences
IL189193A0 (en) Transplastomic plants expressing lumen-targeted protein
WO2004072294A3 (en) Methods and means for nucleic acid sequencing
WO2005007814A3 (en) Genome mapping of functional dna elements and cellular proteins
WO2007005645A3 (en) Materials and methods for the generation of fully 2'-modified nucleic acid transcripts
MX338183B (es) Polipeptidos aislados, polinucleotidos que los codifican, plantas transgenicas quie expresan los mismos y metodos para usarlos.
WO2009148560A8 (en) Methods and compositions for nucleic acid sequencing
IL188202A0 (en) Methods and compositions for expressing negative-sense viral rna in canine cells
EP2641911A3 (de) Zusammensetzungen und Verfahren zur Neuprogrammierung von Zellen ohne genetische Modifikation
WO2010134939A3 (en) Mammalian genes involved in infection
WO2008073378A3 (en) High throughput dna sequencing method and apparatus
EA201070229A1 (ru) Нуклеотидные последовательности msca1, влияющие на мужскую фертильность растений, и способ их применения
WO2005030928A3 (en) PORCINE INVARIANT CHAIN PROTEIN, FULL LENGTH cDNA, GENOMIC ORGANIZATION, AND REGULATORY REGION
WO2007076143A3 (en) Species-specific primer sets and identification of species-specific dna sequences using genome fragment enrichment
WO2006076628A3 (en) Compositions comprising promoter sequences and methods of use
CY1116649T1 (el) Τεχνητη dna αλληλουχια με βελτιστοποιημενη λειτουργια οδηγου σε 5' (5-utr) για βελτιωμενη εκφραση ετερολογων πρωτεϊνων σε φυτα
GB0406863D0 (en) Nucleic acid sequencing
WO2005111204A3 (en) Porcine forssman synthetase cdna and polypeptide
WO2008003666A8 (en) Transplastomic plants expressing lumen-targeted protein
WO2006054297A3 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
WO2006081463A3 (en) Thermococcus zilligii dna polymerases and variants thereof
IN2012DN00172A (de)
WO2010039189A3 (en) Methods for sequencing degraded or modified nucleic acids

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties