[go: up one dir, main page]

ATE414167T1 - Fen-1 endonukleasen, gemische und spaltmethoden - Google Patents

Fen-1 endonukleasen, gemische und spaltmethoden

Info

Publication number
ATE414167T1
ATE414167T1 AT97952237T AT97952237T ATE414167T1 AT E414167 T1 ATE414167 T1 AT E414167T1 AT 97952237 T AT97952237 T AT 97952237T AT 97952237 T AT97952237 T AT 97952237T AT E414167 T1 ATE414167 T1 AT E414167T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid sequences
cliping
fen
endonucleases
Prior art date
Application number
AT97952237T
Other languages
English (en)
Inventor
Michael Kaiser
Victor Lyamichev
Natasha Lyamicheva
Original Assignee
Third Wave Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/757,653 external-priority patent/US5843669A/en
Priority claimed from US08/758,314 external-priority patent/US6090606A/en
Application filed by Third Wave Tech Inc filed Critical Third Wave Tech Inc
Application granted granted Critical
Publication of ATE414167T1 publication Critical patent/ATE414167T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT97952237T 1996-11-29 1997-11-26 Fen-1 endonukleasen, gemische und spaltmethoden ATE414167T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/757,653 US5843669A (en) 1996-01-24 1996-11-29 Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases
US08/758,314 US6090606A (en) 1996-01-24 1996-12-02 Cleavage agents

Publications (1)

Publication Number Publication Date
ATE414167T1 true ATE414167T1 (de) 2008-11-15

Family

ID=27116427

Family Applications (2)

Application Number Title Priority Date Filing Date
AT97952237T ATE414167T1 (de) 1996-11-29 1997-11-26 Fen-1 endonukleasen, gemische und spaltmethoden
AT08019704T ATE530652T1 (de) 1996-11-29 1997-11-26 Fen-1-endonucleasen, mischungen und spaltungsverfahren

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT08019704T ATE530652T1 (de) 1996-11-29 1997-11-26 Fen-1-endonucleasen, mischungen und spaltungsverfahren

Country Status (10)

Country Link
US (2) US6562611B1 (de)
EP (3) EP2055781B1 (de)
JP (4) JP4362150B2 (de)
AT (2) ATE414167T1 (de)
AU (1) AU737449B2 (de)
CA (1) CA2273204C (de)
DE (1) DE69739108D1 (de)
DK (1) DK0966542T3 (de)
ES (2) ES2375764T3 (de)
WO (1) WO1998023774A1 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
ES2375764T3 (es) * 1996-11-29 2012-03-06 Third Wave Technologies, Inc. Endonucleasas fen-1, mezclas y procedimientos de escisión.
CA2289872C (en) 1997-05-05 2007-07-31 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US6194149B1 (en) 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US6210880B1 (en) 1997-09-19 2001-04-03 Third Wave Technologies, Inc. Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
DE60040912D1 (de) * 1999-05-24 2009-01-08 Invitrogen Corp Ein verfahren zum entschützen markierter oligonukleotide
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7534568B2 (en) 1999-10-29 2009-05-19 Hologic Inc. Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
DE60133992D1 (de) * 2000-02-17 2008-06-26 Qiagen Gmbh Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
WO2001071043A1 (en) * 2000-03-22 2001-09-27 Quantum Dot Corporation Loop probe hybridization assay for polynucleotide analysis
CA2403890A1 (en) * 2000-03-27 2001-10-04 Applera Corporation Improved invasion assay
JP2004521606A (ja) * 2000-05-24 2004-07-22 サード・ウェーブ・テクノロジーズ・インク Ｒｎａの検出法
US6511810B2 (en) 2000-07-03 2003-01-28 Applera Corporation Polynucleotide sequence assay
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
EP1548130B1 (de) * 2000-11-15 2012-01-18 Third Wave Technologies, Inc. FEN Endonukleasen
DE10131441A1 (de) * 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7312033B2 (en) 2002-11-14 2007-12-25 Third Wave Technologies, Inc. CFTR allele detection assays
EP1573009B1 (de) 2002-12-18 2011-09-21 Third Wave Technologies, Inc. Nachweis kleiner nukleinsäuren
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US7700750B2 (en) 2003-01-09 2010-04-20 Third Wave Technologies, Inc. Connexin allele detection assays
US20040161749A1 (en) 2003-01-24 2004-08-19 Hall Jeff G. 3' end tagged oligonucleotides
ES2391076T3 (es) 2003-07-29 2012-11-21 Otsuka Pharmaceutical Co., Ltd. Un kit para juzgar el riesgo de granulocitopenia inducida por fármacos
WO2005067643A2 (en) 2004-01-07 2005-07-28 Third Wave Technologies, Inc. Determination of hepatitis c virus genotype
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
CN102242196A (zh) 2004-10-28 2011-11-16 大塚制药株式会社 对干扰素治疗肾细胞癌反应的鉴定标记
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US7960357B2 (en) 2005-10-07 2011-06-14 California Institute Of Technology PKR activation via hybridization chain reaction
US7482127B2 (en) 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides
WO2007111324A1 (ja) * 2006-03-29 2007-10-04 Kyoto University 遺伝子中の５－メチルシトシン検出方法および検出キット
CA2654016C (en) 2006-06-01 2016-01-05 Third Wave Technologies, Inc. Detection of nucleic acids
US7759062B2 (en) 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
CA2665188C (en) 2006-10-04 2014-08-19 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
US9217151B2 (en) 2007-05-16 2015-12-22 California Institute Of Technology Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US8497364B2 (en) 2008-02-27 2013-07-30 California Institute Of Technology Triggered RNAi
CN102301000B (zh) 2008-03-15 2015-04-29 豪洛捷公司 核酸分子扩增反应的分析组分及方法
US20090246788A1 (en) 2008-04-01 2009-10-01 Roche Nimblegen, Inc. Methods and Assays for Capture of Nucleic Acids
US20110136118A1 (en) * 2008-06-17 2011-06-09 Sigma-Aldrich Co. Real time polymerase chain reaction process using a universal detection system
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8715937B2 (en) 2010-11-15 2014-05-06 Exact Sciences Corporation Mutation detection assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
MX2017015093A (es) 2011-01-11 2023-03-10 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
WO2012134195A2 (en) 2011-03-29 2012-10-04 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
CA2849020C (en) 2011-10-18 2023-05-09 Exact Sciences Corporation Multiplexed kras mutation detection assay
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
US9650665B2 (en) 2012-03-05 2017-05-16 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by PTO cleavage and extension assay
WO2014158628A1 (en) 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
US9856472B2 (en) 2013-07-01 2018-01-02 California Institute Of Technology Small conditional RNAs
EP3722442B1 (de) 2013-08-05 2023-04-05 Twist Bioscience Corporation De-novo-synthetisierte genbanken
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2016179093A1 (en) 2015-05-01 2016-11-10 Gen-Probe Incorporated Multiplex invasive cleavage assays
CA2998169A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
AU2017291727B2 (en) 2016-07-05 2021-07-08 California Institute Of Technology Fractional initiator hybridization chain reaction
CN109996876A (zh) 2016-08-22 2019-07-09 特韦斯特生物科学公司 从头合成的核酸文库
AU2017321492A1 (en) 2016-08-30 2019-03-14 California Institute Of Technology Immunohistochemistry via hybridization chain reaction
CN110248724B (zh) 2016-09-21 2022-11-18 特韦斯特生物科学公司 基于核酸的数据存储
CN110366613A (zh) 2016-12-16 2019-10-22 特韦斯特生物科学公司 免疫突触的变体文库及其合成
SG11201907713WA (en) 2017-02-22 2019-09-27 Twist Bioscience Corp Nucleic acid based data storage
AU2018234629A1 (en) 2017-03-15 2019-10-17 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN107012241B (zh) * 2017-05-09 2020-11-20 东莞微量精准检测研究院有限公司 一种U6、miR 92a及miR 21的三重RT-qPCR检测方法及试剂盒
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2019026884A1 (ja) * 2017-07-31 2019-02-07 凸版印刷株式会社 核酸検出方法および検出用試薬
EP4219766A3 (de) 2017-08-11 2023-10-11 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von staphylococcus aureus
WO2019051501A1 (en) 2017-09-11 2019-03-14 Twist Bioscience Corporation PROTEINS BINDING TO GPCR AND METHODS OF SYNTHESIS
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
EP3697529B1 (de) 2017-10-20 2023-05-24 Twist Bioscience Corporation Beheizte nanowells für polynukleotidsynthese
US11662281B2 (en) 2017-12-13 2023-05-30 Gen-Probe Incorporated Compositions and methods for biological sample processing
EP3724354A1 (de) 2017-12-15 2020-10-21 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von toxigenem clostridium difficile
CA3088911A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based storage device and method for synthesizing polynucleotides using the device
IL278771B2 (en) 2018-05-18 2025-09-01 Twist Bioscience Corp Polynucleotides, reagents and methods for nucleic acid hybridization
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
AU2020210753A1 (en) 2019-01-25 2021-09-02 Gen-Probe Incorporated Detection of drug-resistant mycoplasma genitalium
US11492728B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
KR20210143766A (ko) 2019-02-26 2021-11-29 트위스트 바이오사이언스 코포레이션 Glp1 수용체에 대한 변이체 핵산 라이브러리
JP2022550497A (ja) 2019-06-21 2022-12-02 ツイスト バイオサイエンス コーポレーション バーコードに基づいた核酸配列アセンブリ
WO2021055508A1 (en) 2019-09-16 2021-03-25 Exact Sciences Development Company, Llc Structure and temperature-dependent flap endonuclease substrates
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
EP4464827A3 (de) 2019-09-23 2025-02-26 Twist Bioscience Corporation Varianten von nukleinsäurebibliotheken für crth2
WO2022164796A1 (en) 2021-01-26 2022-08-04 California Institute Of Technology Allosteric conditional guide rnas for cell-selective regulation of crispr/cas
CN120442759A (zh) 2021-09-30 2025-08-08 简·探针公司 可温度选择的fret盒信号传导
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511502A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4775619A (en) 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
DE68911648T2 (de) 1988-03-24 1994-06-23 Univ Iowa Res Found Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird.
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
WO1990010691A1 (en) 1989-03-10 1990-09-20 Grace-Sierra Horticultural Products Company Tissue culturing
US5144019A (en) 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA
CA1337639C (en) 1989-08-01 1995-11-28 Joseph Eugene Celebuski Dna probe assay using neutrally charged probe strands
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
ATE169337T1 (de) 1989-12-22 1998-08-15 Hoffmann La Roche Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK0550687T3 (da) 1990-09-28 1999-12-13 Hoffmann La Roche 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser
US6083686A (en) 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
WO1997027214A1 (en) * 1996-01-24 1997-07-31 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5888780A (en) 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5422253A (en) 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
AU8102694A (en) 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
DE69531612D1 (de) 1994-04-25 2003-10-02 Avitech Diagnostics Inc Die bestimmung von mutationen durch spaltung mit resolvase
EP0705905B1 (de) 1994-07-16 2001-10-10 Roche Diagnostics GmbH Verfahren zum sensitiven Nachweis von Nukleinsäuren
JPH10509322A (ja) * 1994-11-09 1998-09-14 サード ウェーヴ テクノロジーズ，インコーポレーテッド 核酸変異体および病原体の迅速な検出および同定
DE4441626A1 (de) 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US5545729A (en) 1994-12-22 1996-08-13 Hybridon, Inc. Stabilized ribozyme analogs
EP0842294B1 (de) 1994-12-23 2002-10-23 Dade Behring Inc. Nachweis von nukleinsäuren durch nuklease-katalysierte produktbildung
US5874283A (en) 1995-05-30 1999-02-23 John Joseph Harrington Mammalian flap-specific endonuclease
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
ES2375764T3 (es) * 1996-11-29 2012-03-06 Third Wave Technologies, Inc. Endonucleasas fen-1, mezclas y procedimientos de escisión.

Also Published As

Publication number Publication date
AU737449B2 (en) 2001-08-23
WO1998023774A1 (en) 1998-06-04
EP2055781A2 (de) 2009-05-06
EP0966542B1 (de) 2008-11-12
JP4191228B2 (ja) 2008-12-03
JP2001526526A (ja) 2001-12-18
EP2055781A3 (de) 2009-10-21
US6562611B1 (en) 2003-05-13
ATE530652T1 (de) 2011-11-15
AU5589898A (en) 1998-06-22
US6555357B1 (en) 2003-04-29
CA2273204C (en) 2008-10-14
JP2008206521A (ja) 2008-09-11
ES2375764T3 (es) 2012-03-06
EP2055781B1 (de) 2011-10-26
JP2007259869A (ja) 2007-10-11
EP2374890B1 (de) 2012-11-14
JP4362150B2 (ja) 2009-11-11
JP2009297032A (ja) 2009-12-24
EP2374890A3 (de) 2011-11-09
HK1163158A1 (en) 2012-09-07
HK1130837A1 (en) 2010-01-08
EP2374890A2 (de) 2011-10-12
DE69739108D1 (de) 2008-12-24
CA2273204A1 (en) 1998-06-04
ES2317658T3 (es) 2009-04-16
DK0966542T3 (da) 2009-03-23
EP0966542A1 (de) 1999-12-29
EP0966542A4 (de) 2004-04-14

Similar Documents

Publication Publication Date Title
ATE530652T1 (de) Fen-1-endonucleasen, mischungen und spaltungsverfahren
DK1634890T3 (da) Invasiv klövning af nukleinsyrer
WO2002070755A3 (en) Fen endonucleases
EP1504093A4 (de) Rna-nachweisenzyme
ATE274587T1 (de) 5'-nukleasen aus thermostabilen dna-polymerasen
WO2001090337A3 (en) Detection of rna
BR9510003A (pt) Moléculas de dna enzimático
WO1999037806A3 (en) Modified nucleic acid probes and uses thereof
EP0266787A3 (de) Verfahren zum Nachweis von Restriktionsfragmentlängenpolymorphien in eukaryotischen Genomen
EP2058407A3 (de) Invasive Spaltung von Nukleinsäuren
EP0760002A4 (de) Die bestimmung von mutationen durch spaltung mit resolvase
ATE380883T1 (de) Direkte multiplex charakterisierung von genomischer dna
CA2284552A1 (en) Detection of nucleic acids by multiple sequential invasive cleavages
CA2171110A1 (en) Detection of amplified or deleted chromosomal regions
WO1997026270A3 (en) Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules
DE69937108D1 (de) Verbindungen und Methoden zum Nachweis von Biomolekülen
ID22339A (id) Campuran-campuran dapat terhubung-silang dan proses pembuatannya
DE59712415D1 (de) Verfahren zur amplifikation von nukleinsäure
NO994254D0 (no) FremgangsmÕte for fjerning av faste asfaltrester produsert i petroleumsindustrien
ID16617A (id) Proses penggilingan basah dan penggiling untuk melakukan proses tersebut
ATE362986T1 (de) High fidelity thermostabile ligase und ihre verwendung
DE69636917D1 (de) Nucleinsäuresonden und Amplifizierungsoligonukleotide für Neisseria Spezies
FR2750999B1 (fr) Adn polymerase thermostable d'archaebacteries du genre pyrococcus sp
UA70937C2 (uk) Спосіб кількісної оцінки реакції в твердій фазі та фторовмісний компонент реакції
ID24012A (id) Proses untuk mencegah pembentukan kerak silika dan alatnya

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties