[go: up one dir, main page]

ATE323169T1 - Plasmid zur verabreichung von nukleine säuren und verwendungsverfahren - Google Patents

Plasmid zur verabreichung von nukleine säuren und verwendungsverfahren

Info

Publication number
ATE323169T1
ATE323169T1 AT96914556T AT96914556T ATE323169T1 AT E323169 T1 ATE323169 T1 AT E323169T1 AT 96914556 T AT96914556 T AT 96914556T AT 96914556 T AT96914556 T AT 96914556T AT E323169 T1 ATE323169 T1 AT E323169T1
Authority
AT
Austria
Prior art keywords
plasmid
nucleic acids
constructs
administration
nonviral
Prior art date
Application number
AT96914556T
Other languages
English (en)
Inventor
Eric M Eastman
Ross H Durland
Original Assignee
Valentis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valentis Inc filed Critical Valentis Inc
Application granted granted Critical
Publication of ATE323169T1 publication Critical patent/ATE323169T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2820/00—Vectors comprising a special origin of replication system
    • C12N2820/002—Vectors comprising a special origin of replication system inducible or controllable
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2820/00—Vectors comprising a special origin of replication system
    • C12N2820/55—Vectors comprising a special origin of replication system from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/36—Vector systems having a special element relevant for transcription being a transcription termination element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT96914556T 1995-06-07 1996-04-23 Plasmid zur verabreichung von nukleine säuren und verwendungsverfahren ATE323169T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/484,723 US5763270A (en) 1995-06-07 1995-06-07 Plasmid for delivery of nucleic acids to cells and methods of use

Publications (1)

Publication Number Publication Date
ATE323169T1 true ATE323169T1 (de) 2006-04-15

Family

ID=23925336

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96914556T ATE323169T1 (de) 1995-06-07 1996-04-23 Plasmid zur verabreichung von nukleine säuren und verwendungsverfahren

Country Status (9)

Country Link
US (2) US5763270A (de)
EP (1) EP0832264B1 (de)
JP (1) JP4335309B2 (de)
AT (1) ATE323169T1 (de)
AU (1) AU711187B2 (de)
CA (1) CA2222601C (de)
DE (1) DE69636032T2 (de)
PT (1) PT832264E (de)
WO (1) WO1996040952A1 (de)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4428402A1 (de) 1994-08-11 1996-02-15 Boehringer Mannheim Gmbh Gentherapeutisches Verfahren unter Verwendung von DNA-Vektoren ohne Selektionsmarker-Gen
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
GB9518395D0 (en) * 1995-09-08 1995-11-08 Therexsys Ltd Plasmid stabilization
US6743780B1 (en) 1995-09-08 2004-06-01 Cobra Biologics Limited Plasmid stabilization
FR2738842B1 (fr) * 1995-09-15 1997-10-31 Rhone Poulenc Rorer Sa Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
US20030125278A1 (en) * 1997-08-13 2003-07-03 Tang De-Chu C. Immunization of animals by topical applications of a salmonella-based vector
US6716823B1 (en) 1997-08-13 2004-04-06 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom, and uses thereof
US20030045492A1 (en) * 1997-08-13 2003-03-06 Tang De-Chu C. Vaccination by topical application of recombinant vectors
US6348450B1 (en) * 1997-08-13 2002-02-19 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom and uses thereof
US6706693B1 (en) 1997-08-13 2004-03-16 The Uab Research Foundation Vaccination by topical application of genetic vectors
US6316262B1 (en) * 1997-09-19 2001-11-13 Metabolix, Inc. Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids
US8076130B2 (en) * 1998-12-02 2011-12-13 University Of Maryland, Baltimore Plasmid maintenance system for antigen delivery
US6413768B1 (en) 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
ES2286905T3 (es) * 1998-12-02 2007-12-01 University Of Maryland, Baltimore Sistema de estabilizacion de plasmidos para el suministro de antigenos.
US20040009936A1 (en) * 1999-05-03 2004-01-15 Tang De-Chu C. Vaccine and drug delivery by topical application of vectors and vector extracts
AU2002322544A1 (en) * 2001-07-18 2003-03-03 Expressive Constructs, Inc. A vector containing an enhanced p15a origin of replication
US20040235121A1 (en) * 2002-08-29 2004-11-25 Integrigen, Inc. High copy number plasmids and their derivatives
US20050026859A1 (en) * 2002-11-12 2005-02-03 John Hilfinger Methods and compositions of gene delivery agents for systemic and local therapy
US20080026077A1 (en) * 2002-11-12 2008-01-31 John Hilfinger Methods and compositions of gene delivery agents for systemic and local therapy
US7261882B2 (en) 2003-06-23 2007-08-28 Reagents Of The University Of Colorado Methods for treating neuropathic pain by administering IL-10 polypeptides
CN101437547A (zh) * 2005-01-20 2009-05-20 自然科技公司 用于遗传免疫的载体和方法
CN101163494A (zh) * 2005-02-23 2008-04-16 Uab研究基金会 烷基糖苷增强的接种
US20060216315A1 (en) * 2005-03-16 2006-09-28 Yoo Tai J Cockroach allergen gene expression and delivery systems and uses
PL2816118T3 (pl) 2005-05-31 2019-03-29 The Regents Of The University Of Colorado, A Body Corporate Sposoby dostarczania genów
JP4528972B2 (ja) * 2005-07-11 2010-08-25 国立大学法人鳥取大学 染色体組み込みマンノース発酵性ザイモモナス属細菌
US8227437B2 (en) * 2006-06-22 2012-07-24 Tai June Yoo Restoration of hearing loss
KR20140102327A (ko) * 2007-02-06 2014-08-21 유태준 유전자 요법을 사용한 신경퇴행성 질환의 치료 및 예방
US9428565B2 (en) 2011-01-31 2016-08-30 The General Hospital Corporation Treatment and bioluminescent visualization using multimodal TRAIL molecules
WO2012177906A1 (en) 2011-06-21 2012-12-27 Alnylam Pharmaceuticals, Inc. Assays and methods for determining activity of a therapeutic agent in a subject
WO2014035474A1 (en) 2012-08-30 2014-03-06 The General Hospital Corporation Compositions and methods for treating cancer
EP2895191B1 (de) 2012-09-14 2019-06-05 The U.S.A. as represented by the Secretary, Department of Health and Human Services Brachyury-protein, adenovirale vektoren zur codierung von brachyury-protein und deren verwendung
EP3323884A1 (de) 2013-02-01 2018-05-23 The United States Of America as Represented by the Secretary, Department of Health an Human Service Verfahren zur erzeugung von zellen im retinalen pigmentepithel aus induzierten pluripotenten stammzellen
WO2014145042A1 (en) 2013-03-15 2014-09-18 Loma Linda University Treatment of autoimmune diseases
SG11201508297YA (en) 2013-04-17 2015-11-27 Genzyme Corp Compositions and methods for treating and preventing macular degeneration
AU2014268603B2 (en) 2013-05-21 2018-03-22 President And Fellows Of Harvard College Engineered heme-binding compositions and uses thereof
CN106163573A (zh) 2014-02-06 2016-11-23 建新公司 用于治疗和预防黄斑变性的组合物及方法
US20180112006A1 (en) 2015-04-17 2018-04-26 The General Hospital Corporation Agents, systems and methods for treating cancer
JP7048482B2 (ja) 2015-08-03 2022-04-05 アメリカ合衆国 Ｂｒａｃｈｙｕｒｙ欠失変異体、Ｂｒａｃｈｙｕｒｙ欠失変異体をコードする非酵母ベクター、及びそれらの使用
AU2016321170B2 (en) 2015-09-08 2022-09-01 Fujifilm Cellular Dynamics Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
AU2016318774B2 (en) 2015-09-08 2022-08-18 FUJIFILM Cellular Dynamics, Inc. MACS-based purification of stem cell-derived retinal pigment epithelium
AU2017290805B2 (en) 2016-07-01 2023-11-16 Research Development Foundation Elimination of proliferating cells from stem cell-derived grafts
EP3491134B1 (de) 2016-08-01 2023-10-11 University of Pittsburgh - of The Commonwealth System of Higher Education Menschliche induzierte pluripotente stammzellen zur hocheffizienten genmanipulation
US10961505B2 (en) 2016-10-05 2021-03-30 FUJIFILM Cellular Dynamics, Inc. Generating mature lineages from induced pluripotent stem cells with MECP2 disruption
CA3043194A1 (en) 2016-11-09 2018-05-17 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services 3d vascularized human ocular tissue for cell therapy and drug discovery
CA3051442A1 (en) 2017-02-14 2018-08-23 Alejandro Soto-Gutierrez Methods of engineering human induced pluripotent stem cells to produce liver tissue
IL269716B1 (en) 2017-04-18 2025-03-01 Fujifilm Cellular Dynamics Inc Activated cells specific antibody immunity
CN112204134A (zh) 2018-04-20 2021-01-08 富士胶片细胞动力公司 眼细胞的分化方法及其用途
WO2020086408A1 (en) 2018-10-26 2020-04-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services A high-yield perfusion-based transient gene expression bioprocess
WO2020106622A1 (en) 2018-11-19 2020-05-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biodegradable tissue replacement implant and its use
US20220031749A1 (en) 2018-11-28 2022-02-03 Board Of Regents, The University Of Texas System Multiplex genome editing of immune cells to enhance functionality and resistance to suppressive environment
US20220033778A1 (en) 2018-11-29 2022-02-03 Board Of Regents, The University Of Texas System Methods for ex vivo expansion of natural killer cells and use thereof
MX2022015012A (es) 2020-05-29 2023-03-03 Fujifilm Cellular Dynamics Inc Epitelio pigmentado de retina y agregados de celulas duales fotorreceptoras y metodos de uso de los mismos.
MX2022015002A (es) 2020-05-29 2023-03-03 Fujifilm Cellular Dynamics Inc Bicapa del epitelio pigmentario de la retina y fotorreceptores y uso de los mismos.
CA3220168A1 (en) 2021-05-26 2022-12-01 Sarah DICKERSON Methods to prevent rapid silencing of genes in pluripotent stem cells
AU2022282379A1 (en) 2021-05-28 2023-11-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biodegradable tissue scaffold with secondary matrix to host weakly adherent cells
AU2022280062A1 (en) 2021-05-28 2023-11-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods to generate macular, central and peripheral retinal pigment epithelial cells
AU2022343749A1 (en) 2021-09-13 2024-03-28 FUJIFILM Cellular Dynamics, Inc. Methods for the production of committed cardiac progenitor cells
KR20250038661A (ko) 2022-06-29 2025-03-19 후지필름 홀딩스 아메리카 코포레이션 Ipsc-유래된 성상 세포 및 이의 사용 방법
WO2024073776A1 (en) 2022-09-30 2024-04-04 FUJIFILM Cellular Dynamics, Inc. Methods for the production of cardiac fibroblasts
WO2024192329A1 (en) 2023-03-16 2024-09-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods for producing stable human chondroctyes and their use for promoting cartillage growth and repair

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5019506A (en) * 1986-05-21 1991-05-28 University College, Cork Plasmid and uses thereof
HU197595B (en) * 1987-09-16 1989-04-28 Richter Gedeon Vegyeszet Process for producing expression vectors
US4956288A (en) * 1988-04-22 1990-09-11 Biogen, Inc. Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
DE3819463A1 (de) * 1988-06-08 1989-12-14 Behringwerke Ag Expressionsvektoren zur herstellung unfusionierter proteine in mikroorganismen
US5492890A (en) * 1990-12-03 1996-02-20 The Scripps Research Institute Polypeptides for promoting cell attachment
US5308760A (en) * 1992-01-10 1994-05-03 Washington Research Foundation Crystal proteins of Bacillus thuringiensis, genes encoding them, and host expressing them
GB9312070D0 (en) * 1993-06-11 1993-07-28 Thrombosis Res Inst Untranslated exon-1 sequences of eukaryotic genes as promoters
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
GB9518395D0 (en) * 1995-09-08 1995-11-08 Therexsys Ltd Plasmid stabilization

Also Published As

Publication number Publication date
DE69636032D1 (de) 2006-05-24
PT832264E (pt) 2006-10-31
US5763270A (en) 1998-06-09
AU711187B2 (en) 1999-10-07
EP0832264B1 (de) 2006-04-12
US6103470A (en) 2000-08-15
JP4335309B2 (ja) 2009-09-30
WO1996040952A1 (en) 1996-12-19
CA2222601C (en) 2012-07-10
AU5788096A (en) 1996-12-30
EP0832264A1 (de) 1998-04-01
DE69636032T2 (de) 2006-11-23
CA2222601A1 (en) 1996-12-19
JPH11511009A (ja) 1999-09-28

Similar Documents

Publication Publication Date Title
ATE323169T1 (de) Plasmid zur verabreichung von nukleine säuren und verwendungsverfahren
ZA961484B (en) Dna molecules preparation and use in gene therapy
HUP0004728A1 (hu) Javított eljárás nukleinsav bevitelére többsejtes eukarióta szervezet sejtjeibe és kombinált alkalmazása
HUP0004589A1 (hu) Javított eljárás nukleinsav bevitelére harántcsikolt izomba és kombinált alkalmazása
WO1994029469A3 (en) Plasmids suitable for gene therapy
CY1106216T1 (el) 5´ ESTs ΓΙΑ ΜΗ ΙΣΤΟ-ΕΙΔΙΚΕΣ ΕΚΚΡΙΤΙΚΕΣ ΠΡΩΤΕΪΝΕΣ
CA2293612A1 (en) Regulation of transcription in mammalian cells and viral replication by a tetracyclin repressor
AU2790292A (en) Viral recombinant vectors for expression in muscle cells
MY134810A (en) Targeted gene expression of thrombopoietein, dnase i and b-interferon
ATE335831T1 (de) Nukleinsäurevektor, einen solchen vektor enthaltene verbindung und impfstoff zur immunisierung gegen hepatitis
NO176921C (no) Genteknologisk fremgangsmåte for fremstilling av et tPA-lignende protein, samt vertscelle som er i stand til å uttrykke et slikt protein
MX9708854A (es) Terapia de genes para regulacion de celulas efectoras.
IL181695A0 (en) Methods for cultivating cells and propagating viruses
FI973309A0 (fi) Menetelmä yhdistelmä-DNA-adenovirusgenomin valmistamiseksi
ATE307886T1 (de) Nukleotidsequenzen, proteine, medikamente und diagnoseagentien zur anwendung in krebsbehandlung
EP0848063A3 (de) Enthaltende hybride Promotoren Nukleinsäurekonstruktionen zur Verwendung in Gentherapie
WO1999047678A3 (en) Interferon alpha plasmids and delivery systems, and methods of making and using the same
HUP9800635A2 (hu) In vivo exogén transzfekcióra és expresszióra használható gyógyászati kombináció
NZ504445A (en) Nucleic acid sequences of genes of the high mobility group proteins and their use in treating tumours, vascular disorders and as a contraceptive
WO1995034669A3 (en) Retroviral gene therapy vectors and therapeutic methods based thereon
HUP0002913A2 (hu) Eljárások és készítmények szekretált fehérjéket, például béta-interferont kódoló géneket alkalmazó terápiára
EP0763205A4 (de) Nukleinsäuresequenzen die lungenzellenspezifishe genexpression kontrollieren
HUP0100776A2 (hu) Gyulladásos sejtek önszabályzott apoptózisa génterápiával
ATE101198T1 (de) Verfahren zur herstellung von produkten von fremdgenen.
AU6113098A (en) Compositions for treatment of disorders involving programmed cell death

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0832264

Country of ref document: EP