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ATE278966T1 - METHOD FOR DETERMINING THE SUBSTRATE SPECIFICITY OF AN ENZYMATIC ACTIVITY AND DEVICE THEREFOR - Google Patents

METHOD FOR DETERMINING THE SUBSTRATE SPECIFICITY OF AN ENZYMATIC ACTIVITY AND DEVICE THEREFOR

Info

Publication number
ATE278966T1
ATE278966T1 AT02735267T AT02735267T ATE278966T1 AT E278966 T1 ATE278966 T1 AT E278966T1 AT 02735267 T AT02735267 T AT 02735267T AT 02735267 T AT02735267 T AT 02735267T AT E278966 T1 ATE278966 T1 AT E278966T1
Authority
AT
Austria
Prior art keywords
enzymatic activity
determining
assembly
substrate specificity
amino acid
Prior art date
Application number
AT02735267T
Other languages
German (de)
Inventor
Jens Schneider-Mergener
Mike Schutkowski
Ulf Reimer
Liying Dong
Soeren Panse
Dirk Scharn
Frank Osterkamp
Gerd Hummel
Laurence Jobron
Original Assignee
Jerini Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jerini Ag filed Critical Jerini Ag
Application granted granted Critical
Publication of ATE278966T1 publication Critical patent/ATE278966T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to a method for determining the substrate specificity of an enzymatic activity comprising the following steps: providing an assembly comprising a plurality of amino acid sequences on a planar surface of a support material, whereby the amino acids are directionally immobilized; contacting and/or incubating of an enzymatic activity with the assembly; and detection of a reaction between one of the amino acid sequences that are immobilized on the assembly and the enzymatic activity. According to the invention, during the reaction of the enzymatic activity with the assembly, a change in the molecular weight of at least one of the amino acid sequences takes place.
AT02735267T 2001-04-17 2002-04-17 METHOD FOR DETERMINING THE SUBSTRATE SPECIFICITY OF AN ENZYMATIC ACTIVITY AND DEVICE THEREFOR ATE278966T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10118774A DE10118774A1 (en) 2001-04-17 2001-04-17 Method for determining the substrate specificity of an enzymatic activity and device therefor
PCT/EP2002/004265 WO2002083933A2 (en) 2001-04-17 2002-04-17 Method for determining the substrate specificity of an enzymatic activity and a device therefor

Publications (1)

Publication Number Publication Date
ATE278966T1 true ATE278966T1 (en) 2004-10-15

Family

ID=7681707

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02735267T ATE278966T1 (en) 2001-04-17 2002-04-17 METHOD FOR DETERMINING THE SUBSTRATE SPECIFICITY OF AN ENZYMATIC ACTIVITY AND DEVICE THEREFOR

Country Status (8)

Country Link
US (2) US20040171097A1 (en)
EP (1) EP1379881B1 (en)
JP (1) JP4588978B2 (en)
AT (1) ATE278966T1 (en)
AU (1) AU2002310855A1 (en)
DE (2) DE10118774A1 (en)
ES (1) ES2230496T3 (en)
WO (1) WO2002083933A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005176613A (en) * 2003-12-16 2005-07-07 Yokogawa Electric Corp Dendrimer DNA extraction method and biochip
US7897354B2 (en) 2005-05-18 2011-03-01 Nuclea Biotechnologies, Inc. Kinase peptides and antibodies
GB0601102D0 (en) 2006-01-19 2006-03-01 Nuclea Biomarkers Llc Kinase Peptides And Antibodies
DE102005051978A1 (en) 2005-10-31 2007-05-10 Forschungszentrum Borstel Zentrum für Medizin und Biowissenschaften Method for determining the cleavability of substrates
US8741849B2 (en) 2007-01-10 2014-06-03 Purdue Research Foundation Kinase inhibitors and uses thereof
AU2007342674B2 (en) * 2007-01-10 2013-09-19 Purdue Research Foundation Polypeptide inhibitors of HSP27 kinase and uses therefor
US20090176664A1 (en) * 2007-06-01 2009-07-09 Keting Chu High density peptide arrays containing kinase or phosphatase substrates
FR2919063B1 (en) 2007-07-19 2009-10-02 Biomerieux Sa METHOD OF DETERMINING LEUCOCYTE ELASTASE INHIBITOR FOR IN VITRO DIAGNOSIS OF COLORECTAL CANCER.
FR2919060B1 (en) * 2007-07-19 2012-11-30 Biomerieux Sa METHOD OF DETERMINING EZRINE FOR IN VITRO DIAGNOSIS OF COLORECTAL CANCER
FR2919065B1 (en) 2007-07-19 2009-10-02 Biomerieux Sa METHOD FOR DETERMINING APOLIPOPROTEIN AI FOR IN VITRO DIAGNOSIS OF COLORECTAL CANCER
JP5715817B2 (en) 2007-07-19 2015-05-13 ビオメリューＢｉｏｍｅｒｉｅｕｘ Method for assay of liver fatty acid binding protein, CEA and CA19-9 for in vitro diagnosis of colorectal cancer
JP4712848B2 (en) * 2008-09-01 2011-06-29 信越化学工業株式会社 Manufacturing method of substrate for producing microarray
AU2009324661A1 (en) 2008-12-10 2011-07-07 Purdue Research Foundation Cell-permeant peptide-based inhibitor of kinases
US9890195B2 (en) 2009-07-27 2018-02-13 Purdue Research Foundation MK2 inhibitor compositions and methods to enhance neurite outgrowth, neuroprotection, and nerve regeneration
EP2316932B1 (en) * 2009-10-29 2013-05-15 Gambro Lundia AB Enzyme-functionalized supports
EP2688904B1 (en) * 2011-03-21 2017-12-27 Atlantic Cancer Research Institute Polypeptides with affinity for heat shock proteins (hsps) and hsp associated complexes (hacs) and their use in diagnosis and therapy
US9452218B2 (en) 2012-03-09 2016-09-27 Purdue Research Foundation Compositions and methods for delivery of kinase inhibiting peptides
US9977019B2 (en) 2012-04-25 2018-05-22 Ms² Array Llc Fluorous peptide microarray, detection process using a fluorous peptide microarray, and process of forming a fluorous peptide microarray
US10443093B2 (en) 2014-10-14 2019-10-15 Ms² Array Llc Fluorous oligonucleotide microarray
CN113174424B (en) * 2021-03-15 2023-05-26 合肥康诺生物制药股份有限公司 Method for detecting enzyme activity in aerobic enzymatic reaction and method for judging fermentation end point of recombinant escherichia coli

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000054046A2 (en) * 1999-03-10 2000-09-14 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services, The National Institutes Of Health Universal protein array system
EP1498480A2 (en) * 1999-09-01 2005-01-19 Incyte Genomics, Inc. Human hydrolytic enzymes
US20010031469A1 (en) * 2000-01-03 2001-10-18 Stefano Volinia Methods for the detection of modified peptides, proteins and other molecules
US20040023245A1 (en) * 2001-06-14 2004-02-05 Au-Young Janice K Protein phosphatases
US6766817B2 (en) * 2001-07-25 2004-07-27 Tubarc Technologies, Llc Fluid conduction utilizing a reversible unsaturated siphon with tubarc porosity action
US20030138895A1 (en) * 2002-07-31 2003-07-24 Tang Y. Tom Drug metabolizing enzymes

Also Published As

Publication number Publication date
WO2002083933A2 (en) 2002-10-24
US20040171097A1 (en) 2004-09-02
US8029979B2 (en) 2011-10-04
WO2002083933A3 (en) 2003-10-16
JP4588978B2 (en) 2010-12-01
US20060234308A1 (en) 2006-10-19
EP1379881B1 (en) 2004-10-06
JP2004534748A (en) 2004-11-18
EP1379881A2 (en) 2004-01-14
AU2002310855A1 (en) 2002-10-28
ES2230496T3 (en) 2005-05-01
DE10118774A1 (en) 2002-10-31
DE50201231D1 (en) 2004-11-11

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