[go: up one dir, main page]

ATE183236T1 - Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung - Google Patents

Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung

Info

Publication number
ATE183236T1
ATE183236T1 AT93203593T AT93203593T ATE183236T1 AT E183236 T1 ATE183236 T1 AT E183236T1 AT 93203593 T AT93203593 T AT 93203593T AT 93203593 T AT93203593 T AT 93203593T AT E183236 T1 ATE183236 T1 AT E183236T1
Authority
AT
Austria
Prior art keywords
pullulanase
relates
dna molecule
microorganisms
produce
Prior art date
Application number
AT93203593T
Other languages
English (en)
Inventor
Philippe Deweer
Antoine Amory
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from BE9201156A external-priority patent/BE1006483A3/fr
Priority claimed from BE9300744A external-priority patent/BE1007313A3/fr
Priority claimed from BE9301278A external-priority patent/BE1007723A6/fr
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE183236T1 publication Critical patent/ATE183236T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Selective Calling Equipment (AREA)
  • Mechanical Means For Catching Fish (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)
AT93203593T 1992-12-28 1993-12-20 Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung ATE183236T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BE9201156A BE1006483A3 (fr) 1992-12-28 1992-12-28 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase, utilisations et compositions comprenant celle-ci.
BE9300744A BE1007313A3 (fr) 1993-07-15 1993-07-15 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.
BE9301278A BE1007723A6 (fr) 1993-11-19 1993-11-19 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.

Publications (1)

Publication Number Publication Date
ATE183236T1 true ATE183236T1 (de) 1999-08-15

Family

ID=27159793

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93203593T ATE183236T1 (de) 1992-12-28 1993-12-20 Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung

Country Status (13)

Country Link
US (6) US5721128A (de)
EP (1) EP0605040B1 (de)
JP (1) JPH06217770A (de)
KR (1) KR100319442B1 (de)
CN (1) CN1061089C (de)
AT (1) ATE183236T1 (de)
AU (2) AU686574B2 (de)
CA (1) CA2112028C (de)
DE (1) DE69325981T2 (de)
DK (1) DK0605040T3 (de)
ES (1) ES2137222T3 (de)
FI (1) FI116531B (de)
MX (1) MX9400123A (de)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69325981T2 (de) * 1992-12-28 2000-02-03 Genencor International, Inc. Pullulanase, Mikroorganismen die sie produzieren, Verfahren zur Herstellung und ihre Anwendung
ATE309373T1 (de) * 1995-09-13 2005-11-15 Genencor Int Alkalophile und thermophile mikroorganismen und damit erhaltene enzymen
US6962263B2 (en) 1996-01-24 2005-11-08 Sambrailo Packaging, Inc. Produce packaging system having produce containers with double-arched ventilation channels
DE19627932A1 (de) * 1996-07-11 1998-01-15 Boehringer Mannheim Gmbh Sensitiver Epstein-Barr-Virus DNA-Nachweis
PT1027367E (pt) 1996-12-06 2005-09-30 Diversa Corp Enzimas de glicosidade
US7279316B2 (en) * 1996-12-06 2007-10-09 Verenium Corporation Enzymes having glycosidase activity and methods of use thereof
CA2321817C (en) 1998-03-04 2011-02-01 Genencor International, Inc. Modified forms of pullulanase
US6639126B1 (en) 1999-12-06 2003-10-28 Pioneer Hi-Bred International, Inc. Production of modified polysaccharides
US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
US7081261B2 (en) * 2002-05-14 2006-07-25 National Starch And Chemical Investment Holding Corporation Resistant starch prepared by isoamylase debranching of low amylose starch
US20040234649A1 (en) * 2003-03-10 2004-11-25 Broin And Associates, Inc. Method for producing ethanol using raw starch
US20050233030A1 (en) * 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
EP1662898A4 (de) * 2003-09-08 2010-09-08 Univ Louisiana State Resistente stärke mit kocheigenschaften ähnlich wie unbehandelte stärke
US20050239181A1 (en) * 2004-03-10 2005-10-27 Broin And Associates, Inc. Continuous process for producing ethanol using raw starch
WO2006119386A2 (en) * 2005-05-02 2006-11-09 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
US8216817B2 (en) 2005-09-20 2012-07-10 Novozymes North America, Inc. Process of producing a fermentation product
US7919289B2 (en) 2005-10-10 2011-04-05 Poet Research, Inc. Methods and systems for producing ethanol using raw starch and selecting plant material
WO2007144393A1 (en) * 2006-06-15 2007-12-21 Novozymes, Inc. Mashing process
US8765199B2 (en) 2006-06-15 2014-07-01 Novozymes A/S Mashing process
EP2662445B1 (de) * 2006-08-04 2017-10-25 Amano Enzyme Inc. Verfahren zur Konstruktion eines mutierten Enzyms, Verfahren zur Herstellung davon und mutiertes Enzym
US9969996B2 (en) 2006-08-04 2018-05-15 Amano Enzyme Inc. Method for making mutated pullulanase enzyme, mutated pullulanase enzyme, and microorganism expressing the same
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
CN101952409B (zh) 2007-12-12 2014-12-10 诺维信公司 酿造方法
CN105623937A (zh) * 2007-12-12 2016-06-01 诺维信公司 糖化方法
EP2346978A2 (de) 2008-10-15 2011-07-27 Novozymes A/S Brauverfahren
US8450094B1 (en) 2009-03-03 2013-05-28 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
US20100233771A1 (en) * 2009-03-03 2010-09-16 Mcdonald William F System for pre-treatment of biomass for the production of ethanol
US8815552B2 (en) * 2009-03-03 2014-08-26 Poet Research, Inc. System for fermentation of biomass for the production of ethanol
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
EP2430176B1 (de) * 2009-05-11 2024-08-07 Danisco US Inc. Verbesserte maltotetraosesirup produktion unter verwendung einer pseudomonas saccharophila maltotetraohydrolase variante und eines "debranching" enzyms
WO2011017093A1 (en) 2009-08-07 2011-02-10 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
CN102639687A (zh) 2009-11-13 2012-08-15 诺维信公司 酿造方法
WO2012140075A2 (en) 2011-04-15 2012-10-18 Novozymes A/S Method for production of brewers wort
EP2761013A1 (de) 2011-09-29 2014-08-06 Danisco US Inc. Verflüssigung und verzuckerung von körniger stärke in hoher konzentration
EP2766490B1 (de) 2011-10-11 2017-04-12 Novozymes North America, Inc. Verflüssigungsverfahren zur herstellung von gärungsprodukten
US20150111259A1 (en) 2012-03-28 2015-04-23 Danisco Us Inc. Method for Making High Maltose Syrup
CN104271729A (zh) 2012-05-11 2015-01-07 诺维信公司 一种酿造方法
WO2014028434A2 (en) 2012-08-16 2014-02-20 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
CN104718289A (zh) 2012-10-17 2015-06-17 诺维信公司 用于生产啤酒麦芽汁的方法
EP2931872B1 (de) 2012-12-11 2018-01-17 Danisco US Inc. Trichoderma-reesei-wirtszellen zur expression einer glukoamylase aus aspergillus fumigatus und verfahren zur verwendung davon
EP2904105A1 (de) 2012-12-20 2015-08-12 Danisco US Inc. Verfahren zur verwendung von alpha-amylase aus aspergillus terreus und pullulanase für eine verzuckerung
WO2014150195A1 (en) * 2013-03-15 2014-09-25 Verenium Corporation Enzymes having pullulanase activity
EP3022300B1 (de) 2013-07-17 2018-07-11 Novozymes A/S Pullanase chimaeren und dafür kodierende nukleinsäuren
CN103571812B (zh) * 2013-11-26 2015-06-24 江南大学 一种分泌效率和热稳定性提高的普鲁兰酶突变体及其制备方法
WO2015110473A2 (en) 2014-01-22 2015-07-30 Novozymes A/S Pullulanase variants and polynucleotides encoding same
BR112016018075B1 (pt) 2014-02-07 2022-01-18 Novozymes A/S Composição compreendendo uma alfa-amilase, uma pululanase e uma enzima glicoamilase e método de fabricação de xarope de glicose a partir de amido liquefeito
WO2016087327A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains
CA2967710A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Improved production of glucose syrups
WO2016126294A1 (en) 2015-02-04 2016-08-11 Nanjing Bestzyme Bio-Engineering Co. Ltd. Truncated pullulanases, methods of production, and methods of use thereof
WO2017014974A1 (en) 2015-07-21 2017-01-26 Novozymes A/S Polypeptides having pullulanase activity suitable for use in liquefaction
EP3825399A1 (de) 2016-06-30 2021-05-26 Danisco US Inc. Aspartat-proteasen
US20190330577A1 (en) 2016-12-21 2019-10-31 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases
RU2019132448A (ru) 2017-03-15 2021-04-15 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы применения сериновой протеазы архей
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
WO2018169784A1 (en) 2017-03-15 2018-09-20 Dupont Nutrition Biosciences Aps Trypsin-like serine proteases and uses thereof cross-reference to related application
EP3665267A1 (de) 2017-08-07 2020-06-17 Novozymes A/S Verwendung von fca-steuerung auf ph-basis
FR3091703B1 (fr) 2019-01-11 2021-02-12 Fermentalg Procédé d’extraction de phycocyanines
FR3091640B1 (fr) 2019-01-11 2021-06-11 Fermentalg Procédé de purification de phycocyanines
FR3092586A1 (fr) 2019-02-08 2020-08-14 Fermentalg Procédé optimisé d’exploitation industrielle d’algues rouges unicellulaires
BR112021018137A2 (pt) 2019-03-18 2022-01-25 Novozymes As Pululanase variante, variante, polinucleotídeo, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, composição, métodos de produção de uma pululanase variante e de produção de um mosto de cerveja, processos de produção de um xarope e de produção de produtos de fermentação, e, uso da célula hospedeira e da pululanase variante
EP4055157A1 (de) 2019-11-08 2022-09-14 Novozymes A/S Stabilisierte flüssige enzymzusammensetzungen zum brauen
CN111560077A (zh) * 2020-05-21 2020-08-21 中国海洋大学 一种酶及其在合成普鲁兰多糖中的作用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
FR3147934A1 (fr) 2023-04-19 2024-10-25 Fermentalg Procede de degradation du glycogene present dans une biomasse de galdieria

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4560651A (en) * 1981-04-20 1985-12-24 Novo Industri A/S Debranching enzyme product, preparation and use thereof
JPS57174089A (en) * 1981-04-20 1982-10-26 Novo Industri As Chain dividing enzyme product
US4628031A (en) * 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
US4628028A (en) * 1985-05-23 1986-12-09 Cpc International Inc. Novel thermostable pullulanase enzyme and method for its production
US4612287A (en) * 1985-05-23 1986-09-16 Cpc International Inc. Plasmids containing a gene coding for a thermostable pullulanase and pullulanase-producing strains of Escherichia coli and Bacillus subtilis containing the plasmids
CA1285896C (en) * 1986-09-26 1991-07-09 Kuniharu Nakai .alpha.-1, 6-GLUCOSIDASE AND PROCESS FOR PRODUCING THE SAME
JPH0223872A (ja) * 1988-07-12 1990-01-26 Ezaki Glico Co Ltd 組換えプラスミド，それにより形質転換された枯草菌及びそれによる耐熱性プルラナーゼの製造法
EP0405283A3 (en) * 1989-06-26 1991-03-06 Enzyme Bio-Systems Ltd. Novel thermoduric and aciduric pullulanase enzyme and method for its production
US5055403A (en) * 1989-06-26 1991-10-08 Enzyme Bio-Systems, Ltd. Thermoduric and aciduric pullulanase enzyme and method for its production
DK47291D0 (da) * 1991-03-15 1991-03-15 Novo Nordisk As Pullulanase
JP3026857B2 (ja) * 1991-07-05 2000-03-27 ナガセ生化学工業株式会社 新規プルラナーゼおよびその製造法
JP3173849B2 (ja) * 1992-02-26 2001-06-04 天野エンザイム株式会社 新規な枝切り酵素及びその製造法
JPH05236959A (ja) * 1992-02-28 1993-09-17 Yoshiyuki Takasaki プルラナーゼ、その製造法及び該酵素を用いる澱粉の 糖化法
DE4244107C2 (de) * 1992-12-24 1996-02-08 Hirschmann Richard Gmbh Co Hochfrequenz-Übertrager
DE69325981T2 (de) * 1992-12-28 2000-02-03 Genencor International, Inc. Pullulanase, Mikroorganismen die sie produzieren, Verfahren zur Herstellung und ihre Anwendung
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

Also Published As

Publication number Publication date
FI935900A0 (fi) 1993-12-28
DK0605040T3 (da) 2000-03-13
JPH06217770A (ja) 1994-08-09
CA2112028C (fr) 2003-03-11
CN1090325A (zh) 1994-08-03
US5731174A (en) 1998-03-24
US6074854A (en) 2000-06-13
AU686574B2 (en) 1998-02-12
KR940014776A (ko) 1994-07-19
EP0605040B1 (de) 1999-08-11
AU5275993A (en) 1994-07-07
FI935900L (fi) 1994-06-29
US5721128A (en) 1998-02-24
AU6483198A (en) 1998-07-30
CN1061089C (zh) 2001-01-24
MX9400123A (es) 1994-07-29
US5817498A (en) 1998-10-06
CA2112028A1 (fr) 1994-06-29
US5721127A (en) 1998-02-24
FI116531B (fi) 2005-12-15
DE69325981D1 (de) 1999-09-16
DE69325981T2 (de) 2000-02-03
ES2137222T3 (es) 1999-12-16
KR100319442B1 (ko) 2002-11-18
US5736375A (en) 1998-04-07
EP0605040A1 (de) 1994-07-06

Similar Documents

Publication Publication Date Title
ATE183236T1 (de) Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung
DE69016012D1 (en) Neue hyperthermostabile alpha - amylase.
KR950032615A (ko) 말토헥사오스 및 말토헵타오스 생성 아밀라아제와 그 제조 방법 및 용도
ATE43634T1 (de) Maltose erzeugendes amylase-enzymprodukt, dessen herstellung und dessen verwendung.
MX9602397A (es) Tratamiento enzimatico del cacao.
DE69508307D1 (de) Verfahren zur Herstellung von stärkehaltigen Produkten
DE69802538D1 (de) Verfahren zur herstellung einer hochkonzentrierten kolloidalen kieselsäure-suspension und des dabei hergestellten produktes
ATE117367T1 (de) Verfahren zur herstellung von hefeextrakt, hefeextrakt, seine verwendung als würzstoff und nahrungsmittel, das den hefeextrakt enthält.
ATE394490T1 (de) Mutante bakteriestämme vom genus sphingonomas die defizient in der herstellung von polyhydroxybutyrate sind, und verfahren zur klärung von sphinganen
ATE371703T1 (de) Modifizierte stärke und verfahren zu deren herstellung
DE59206521D1 (de) Verfahren zur extrazellulären Herstellung von hochmolekularen Homopolysacchariden und zu ihrer Anwendung, sowie die entsprechenden Pilzstämme
AU1176197A (en) Thermolabile uracil-DNA-glycosylase, process for its production and use for removing uracil from DNA
KR920703793A (ko) 효소의 탈목질화 활성을 나타내는 조제물 및 그 제조방법과 적용
ATE234928T1 (de) Hitzebeständige rekombinante enzyme für die umsetzung von maltose in trehalose
ATE280222T1 (de) Alkaline protease, verfahren zu ihrer herstellung,anwendung derselben und diese herstellende mikroorganismen
DE2919622A1 (de) Verfahren zur herstellung wasserloeslicher stabilisierter enzymderivate und deren verwendung
DE69014044D1 (de) Neue alkylische Pullulanase Y mit alpha-Amylase-Aktivität, sie produzierender Mikroorganismus und Verfahren zur Herstellung dieses Enzyms.
DE69624993D1 (de) Hitzebeständige Maltosephosphorylase, Prozess für ihre Herstellung, Bakterien,die für ihre Herstellung verwendet werden,und Methoden zur Verwendung des Enzyms
AU2793095A (en) Method of producing 1,4-butanediol
ATE469235T1 (de) Verfahren zur herstellung von isomaltose und isomaltit sowie verwendung davon
DE59408523D1 (de) Enzymatisches verfahren zur herstellung von dihydroxyacetonphosphat aus glycerinphosphat und seine verwendung in enzymatischen aldoladditionen
DE69520829D1 (de) Herstellung von L - Ascorbinsäure in Mikroorganismen
ATE2339T1 (de) Verfahren zur herstellung von sphingomyelinase, sphingomyelinase und deren verwendung.
DE3332639C2 (de) Verfahren zur Herstellung von Maltopentaose
KR960023051A (ko) 말토테트라오즈 생산 아밀라제의 제조방법

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification