AR126380A1 - INSECT INHIBITOR PROTEINS - Google Patents
INSECT INHIBITOR PROTEINSInfo
- Publication number
- AR126380A1 AR126380A1 ARP220101759A ARP220101759A AR126380A1 AR 126380 A1 AR126380 A1 AR 126380A1 AR P220101759 A ARP220101759 A AR P220101759A AR P220101759 A ARP220101759 A AR P220101759A AR 126380 A1 AR126380 A1 AR 126380A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleic acid
- pesticidal
- recombinant nucleic
- acid molecule
- acid sequence
- Prior art date
Links
- 241000238631 Hexapoda Species 0.000 title 1
- 108091006086 inhibitor proteins Proteins 0.000 title 1
- 230000000361 pesticidal effect Effects 0.000 abstract 11
- 102000004169 proteins and genes Human genes 0.000 abstract 9
- 108090000623 proteins and genes Proteins 0.000 abstract 9
- 150000007523 nucleic acids Chemical group 0.000 abstract 8
- 210000004027 cell Anatomy 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 241000258937 Hemiptera Species 0.000 abstract 3
- 241000255777 Lepidoptera Species 0.000 abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 108091033319 polynucleotide Proteins 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 3
- 239000002157 polynucleotide Substances 0.000 abstract 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 206010061217 Infestation Diseases 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 210000004436 artificial bacterial chromosome Anatomy 0.000 abstract 1
- 210000001106 artificial yeast chromosome Anatomy 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se divulgan proteínas plaguicidas que exhiben actividad tóxica contra especies de plagas de Lepidópteros y Hemípteros, e incluyen, pero no se limitan a, TIC2199. Se proporcionan constructos de ADN que contienen una secuencia de ácido nucleico recombinante que codifica la proteína plaguicida divulgada. Se proporcionan plantas transgénicas, células vegetales, semillas y partes de plantas resistentes a infestaciones de Lepidópteros y Hemípteros que contienen secuencias de ácido nucleico recombinante que codifican las proteínas plaguicidas de la presente invención. Además se proporcionan métodos para detectar la presencia de las secuencias de ácido nucleico recombinante o la proteína de la presente invención en una muestra biológica y métodos para controlar las plagas de especies de Lepidópteros y Hemípteros utilizando la proteína plaguicida TIC2199. Reivindicación 1: Una molécula de ácido nucleico recombinante que comprende un promotor heterólogo unido de forma operativa a un segmento polinucleotídico que codifica una proteína plaguicida o fragmento plaguicida de la misma, en donde, opcionalmente: a. dicha proteína plaguicida comprende la secuencia de aminoácidos de SEQ ID Nº 2, 4, 7, 10, 13, 15 o 17; b. dicha proteína plaguicida comprende una secuencia de aminoácidos que tiene al menos un 96%, o un 97%, o un 98%, o un 99% o aproximadamente un 100% de identidad de secuencia de aminoácidos con las SEQ ID Nº 2, 4, 7, 10, 13, 15 o 17; o c. dicho segmento polinucleotídico se hibrida en condiciones de hibridación estrictas a un polinucleótido que tiene la secuencia de nucleótidos de las SEQ ID Nº 1, 3, 5, 6, 8, 9, 11, 12, 14, 16 o 18. Reivindicación 2: La molécula de ácido nucleico recombinante de la reivindicación 1, en donde: a. dicha molécula de ácido nucleico recombinante se expresa en una célula vegetal para producir una cantidad de eficacia plaguicida de la proteína plaguicida o fragmento plaguicida; o b. dicha molécula de ácido nucleico recombinante está en enlace operable con un vector, y dicho vector se selecciona del grupo que consiste en un plásmido, un fagémido, un bácmido, un cósmido y un cromosoma artificial bacteriano o de levadura. Reivindicación 3: La molécula de ácido nucleico recombinante de la reivindicación 1, presente dentro de una célula huésped, en donde dicha célula huésped se selecciona del grupo que consiste en una célula bacteriana y una célula vegetal.Pesticidal proteins that exhibit toxic activity against Lepidoptera and Hemiptera pest species are disclosed, and include, but are not limited to, TIC2199. DNA constructs containing a recombinant nucleic acid sequence encoding the disclosed pesticidal protein are provided. Transgenic plants, plant cells, seeds and plant parts resistant to Lepidoptera and Hemiptera infestations containing recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention are provided. Additionally provided are methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample and methods for controlling pests of Lepidoptera and Hemiptera species using the pesticidal protein TIC2199. Claim 1: A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a pesticidal protein or pesticidal fragment thereof, wherein, optionally: a. said pesticidal protein comprises the amino acid sequence of SEQ ID NO: 2, 4, 7, 10, 13, 15 or 17; b. said pesticidal protein comprises an amino acid sequence that has at least 96%, or 97%, or 98%, or 99%, or approximately 100% amino acid sequence identity with SEQ ID NO: 2, 4, 7, 10, 13, 15 or 17; or c. said polynucleotide segment hybridizes under strict hybridization conditions to a polynucleotide having the nucleotide sequence of SEQ ID NO: 1, 3, 5, 6, 8, 9, 11, 12, 14, 16 or 18. Claim 2: recombinant nucleic acid molecule of claim 1, wherein: a. said recombinant nucleic acid molecule is expressed in a plant cell to produce a pesticidal efficacy amount of the pesticidal protein or pesticidal fragment; or b. said recombinant nucleic acid molecule is in operable link with a vector, and said vector is selected from the group consisting of a plasmid, a phagemid, a bacmid, a cosmid and a bacterial or yeast artificial chromosome. Claim 3: The recombinant nucleic acid molecule of claim 1, present within a host cell, wherein said host cell is selected from the group consisting of a bacterial cell and a plant cell.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063348278P | 2020-06-02 | 2020-06-02 | |
| US202163219604P | 2021-07-08 | 2021-07-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR126380A1 true AR126380A1 (en) | 2023-10-11 |
Family
ID=88679321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220101759A AR126380A1 (en) | 2020-06-02 | 2022-07-05 | INSECT INHIBITOR PROTEINS |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR126380A1 (en) |
-
2022
- 2022-07-05 AR ARP220101759A patent/AR126380A1/en unknown
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