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AR124409A1 - PLANTS RESISTANT TO HERBICIDES - Google Patents

PLANTS RESISTANT TO HERBICIDES

Info

Publication number
AR124409A1
AR124409A1 ARP210103554A ARP210103554A AR124409A1 AR 124409 A1 AR124409 A1 AR 124409A1 AR P210103554 A ARP210103554 A AR P210103554A AR P210103554 A ARP210103554 A AR P210103554A AR 124409 A1 AR124409 A1 AR 124409A1
Authority
AR
Argentina
Prior art keywords
hppd
amino acid
plant
mutant
herbicide
Prior art date
Application number
ARP210103554A
Other languages
Spanish (es)
Inventor
Caixia Gao
Yuhang Chen
Rui Zhang
Meihua Wang
Sha Chen
Chunrui Zhang
Original Assignee
Suzhou Qi Biodesign Biotechnology Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Qi Biodesign Biotechnology Company Ltd filed Critical Suzhou Qi Biodesign Biotechnology Company Ltd
Publication of AR124409A1 publication Critical patent/AR124409A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención se relaciona con el campo de la ingeniería genética en plantas. Específicamente, la presente invención se relaciona con una p-hidroxifenilpiruvato dioxidasa (HPPD) mutante capaz de conferir resistencia a herbicidas en plantas, y con un método para la producción de una planta resistente a herbicidas que contiene la p-hidroxifenilpiruvato dioxidasa (HPPD) mutante. Reivindicación 1: Una mutante de HPPD o un fragmento funcional de la misma, que tiene una mutación de aminoácidos tal como una sustitución de aminoácidos en una o más posiciones seleccionadas de la posición 417, 365, 378, 414, 415, ó 419 comparada con la HPPD de tipo salvaje, refiriéndose la posición de aminoácidos a la SEQ ID Nº 1. Reivindicación 9: Un ácido nucleico que comprende una secuencia de nucleótidos que codifica la mutante de HPPD o un fragmento funcional de la misma de una cualquiera de las reivindicaciones 1 - 8. Reivindicación 10: Un casete de expresión que comprende una secuencia de nucleótidos que codifica la mutante de HPPD o un fragmento funcional de la misma de una cualquiera de las reivindicaciones 1 - 8 operativamente ligada a una secuencia reguladora. Reivindicación 11: Un constructo de expresión que comprende el casete de expresión de la reivindicación 10. Reivindicación 12: Un método para producir una planta resistente a herbicidas mediante transgén, que comprende introducir el ácido nucleico de la reivindicación 9, el casete de expresión de la reivindicación 10 y/o el constructo de expresión de la reivindicación 11 en la planta. Reivindicación 13: Un método para producir plantas resistentes a herbicidas, el método que comprende la modificación dirigida de la secuencia codificante de HPPD endógena de la planta, dando como resultado una mutación de aminoácidos de la HPPD endógena en una o más posiciones seleccionadas del grupo que consiste en la posición 417, 365, 378, 414, 415 ó 419, refiriéndose la posición de aminoácidos a la SEQ ID Nº 1. Reivindicación 18: Un método para producir una planta resistente a herbicidas, que comprende realizar mutagénesis física o mutagénesis química a una población de dichas plantas, y seleccionar una planta cuya HPPD endógena contiene al menos una mutación de aminoácidos en una o más posiciones, tal como 1, 2, 3, 4, 5 ó 6 posiciones, seleccionadas desde la posición 417, 365, 378, 414, 415 ó 419, refiriéndose la posición de aminoácidos a la SEQ ID Nº 1.The invention relates to the field of plant genetic engineering. Specifically, the present invention relates to a mutant p-hydroxyphenylpyruvate dioxidase (HPPD) capable of conferring herbicide resistance in plants, and to a method for producing a herbicide-resistant plant containing the mutant p-hydroxyphenylpyruvate dioxidase (HPPD). . Claim 1: A mutant of HPPD or a functional fragment thereof, having an amino acid mutation such as an amino acid substitution at one or more positions selected from position 417, 365, 378, 414, 415, or 419 compared to the wild-type HPPD, the amino acid position referring to SEQ ID NO: 1. Claim 9: A nucleic acid comprising a nucleotide sequence encoding the HPPD mutant or a functional fragment thereof of any one of claims 1 - 8. Claim 10: An expression cassette comprising a nucleotide sequence encoding the HPPD mutant or a functional fragment thereof of any one of claims 1-8 operably linked to a regulatory sequence. Claim 11: An expression construct comprising the expression cassette of claim 10. Claim 12: A method of producing a herbicide-resistant plant by transgene, comprising introducing the nucleic acid of claim 9, the expression cassette of the claim 10 and/or the expression construct of claim 11 in the plant. Claim 13: A method of producing herbicide resistant plants, the method comprising the targeted modification of the endogenous HPPD coding sequence of the plant, resulting in an amino acid mutation of the endogenous HPPD at one or more positions selected from the group that consists of position 417, 365, 378, 414, 415 or 419, the amino acid position referring to SEQ ID No. 1. Claim 18: A method of producing a herbicide resistant plant, comprising performing physical mutagenesis or chemical mutagenesis to a population of said plants, and selecting a plant whose endogenous HPPD contains at least one amino acid mutation at one or more positions, such as 1, 2, 3, 4, 5 or 6 positions, selected from position 417, 365, 378 , 414, 415 or 419, the amino acid position referring to SEQ ID No. 1.

ARP210103554A 2020-12-17 2021-12-17 PLANTS RESISTANT TO HERBICIDES AR124409A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011493546 2020-12-17

Publications (1)

Publication Number Publication Date
AR124409A1 true AR124409A1 (en) 2023-03-22

Family

ID=82058943

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP210103554A AR124409A1 (en) 2020-12-17 2021-12-17 PLANTS RESISTANT TO HERBICIDES

Country Status (3)

Country Link
CN (1) CN116724119A (en)
AR (1) AR124409A1 (en)
WO (1) WO2022127894A1 (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102348802B (en) * 2009-01-22 2014-09-24 先正达参股股份有限公司 Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
US9187762B2 (en) * 2010-08-13 2015-11-17 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
CA3055389A1 (en) * 2017-03-07 2018-09-13 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
WO2018205995A1 (en) * 2017-05-11 2018-11-15 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Creation of herbicide resistant gene and use thereof
CN110616203B (en) * 2018-06-04 2022-04-01 青岛清原化合物有限公司 Mutant p-hydroxyphenylpyruvate dioxygenase, nucleic acid encoding same and use thereof
BR112021024656A2 (en) * 2019-09-17 2022-04-12 Beijing Dabeinong Biotechnology Co Ltd Mutant hydroxyphenylpyruvate-dioxygenase polypeptide, coding gene and use thereof

Also Published As

Publication number Publication date
CN116724119A (en) 2023-09-08
WO2022127894A1 (en) 2022-06-23

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