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AR085906A1 - LEAVING LEAVES CAPABLE OF FERMENTING URONIC ACIDS - Google Patents

LEAVING LEAVES CAPABLE OF FERMENTING URONIC ACIDS

Info

Publication number
AR085906A1
AR085906A1 ARP120100845A ARP120100845A AR085906A1 AR 085906 A1 AR085906 A1 AR 085906A1 AR P120100845 A ARP120100845 A AR P120100845A AR P120100845 A ARP120100845 A AR P120100845A AR 085906 A1 AR085906 A1 AR 085906A1
Authority
AR
Argentina
Prior art keywords
pentose
fermentation product
acid
fermenting
uronic
Prior art date
Application number
ARP120100845A
Other languages
Spanish (es)
Inventor
Bont Johannes Adrianus Maria De
Aloysius Wilhelmus Rudolphus Hubertus Teunissen
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of AR085906A1 publication Critical patent/AR085906A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Cepas de levadura manipuladas mediante ingeniería genética para producir productos de fermentación a partir de ácidos urónicos tales como ácido galacturónico o ácido glucurónico. Para ello, la cepa de levadura fue modificada por introducción de a) genes que codifican enzimas de una vía de plantas de tipo salvaje y que tienen la capacidad de convertir un ácido D-urónico en pentosa-1-fosfato y dióxido de carbono; b) un gen que codifica una enzima con capacidad para convertir una pentosa-1P en una pentosa; y c) genes que codifican enzimas con capacidad para convertir una pentosa en al menos uno entre xilulosa y xilulosa 5-fosfato. La presente se relaciona además con el uso de tales cepas de levadura modificadas genéticamente en la producción de productos de fermentación y con un proceso para producir un producto de fermentación. El proceso comprende los pasos de a) fermentar un medio con una célula de levadura de la presente, con lo cual el medio contiene o es alimentado con una fuente de un ácido urónico; y donde la célula de levadura fermenta al ácido urónico en el producto de fermentación; y, opcionalmente, b) recuperar el producto de fermentación. El producto de fermentación preferido es etanol.Yeast strains manipulated by genetic engineering to produce fermentation products from uronic acids such as galacturonic acid or glucuronic acid. For this, the yeast strain was modified by introducing a) genes that encode enzymes from a wild-type plant pathway and that have the ability to convert a D-uronic acid into pentose-1-phosphate and carbon dioxide; b) a gene that encodes an enzyme capable of converting a pentose-1P into a pentose; and c) genes encoding enzymes capable of converting a pentose into at least one between xylulose and xylulose 5-phosphate. This is also related to the use of such genetically modified yeast strains in the production of fermentation products and to a process to produce a fermentation product. The process comprises the steps of a) fermenting a medium with a yeast cell of the present, whereby the medium contains or is fed with a source of a uronic acid; and where the yeast cell ferments uronic acid in the fermentation product; and, optionally, b) recover the fermentation product. The preferred fermentation product is ethanol.

ARP120100845A 2011-03-14 2012-03-14 LEAVING LEAVES CAPABLE OF FERMENTING URONIC ACIDS AR085906A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161452366P 2011-03-14 2011-03-14

Publications (1)

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AR085906A1 true AR085906A1 (en) 2013-11-06

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AR (1) AR085906A1 (en)
WO (1) WO2012125027A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2013012269A (en) * 2011-04-22 2013-11-22 Dsm Ip Assets Bv Yeast cell capable of converting sugars including arabinose and xylose.
CN106191017B (en) * 2015-05-25 2021-12-07 中国医学科学院药物研究所 Uridine-5' -diphosphate apiose/xylose synthetase derived from ornithogalum caudatum, nucleotide sequence and application thereof
EP3519567A4 (en) 2016-10-03 2020-06-03 The Regents of The University of California MODIFIED MICROORGANISMS FOR THE PRODUCTION OF BASIC CHEMICALS AND CELL BIOMASS
EP3906305A1 (en) 2018-12-31 2021-11-10 DSM IP Assets B.V. Novel acetyl-transferases
CN111440782B (en) * 2020-04-22 2021-09-17 青岛大学 Beta-galactosidase GalA and application thereof
CN111748575B (en) * 2020-06-22 2023-03-24 华南农业大学 Method for improving plant saccharification efficiency by down-regulating UXE gene and application thereof
CN111849941A (en) * 2020-08-12 2020-10-30 青岛大学 A novel β-galactosidase and its application in degrading lactose in milk

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
FR2679920A1 (en) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Highly stable recombinant yeasts for the production of recombinant proteins, their preparation and their use
WO2003062430A1 (en) 2002-01-23 2003-07-31 Royal Nedalco B.V. Fermentation of pentose sugars
SE0202090D0 (en) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modified yeast consuming L-arabinose
WO2004099381A2 (en) 2003-05-02 2004-11-18 Cargill Dow Llc Genetically modified yeast species and fermentation processes using genetically modified yeast
SE0302421D0 (en) 2003-09-11 2003-09-11 Forskarpatent I Syd Ab Construction of new xylose utilizing Saccharomyces cerevisiae strain
SE0400815D0 (en) 2004-03-26 2004-03-26 Forskarpatent I Syd Ab Traits in recombinant xylose-growing Saccharomyces cerevisiae strains using genome-wide transcription analysis
SE0401303D0 (en) 2004-05-19 2004-05-19 Forskarpatent I Syd Ab Ethanol productivities of microbial strains in fermentation of dilute-acid hydrolyzates depending on their furan reduction capacities
CA2573483C (en) 2004-07-16 2014-10-28 Technische Universiteit Delft Metabolic engineering of xylose-fermenting eukaryotic cells
US20100304454A1 (en) 2007-07-19 2010-12-02 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
EP2250262B1 (en) 2008-03-07 2015-07-29 DSM IP Assets B.V. A pentose sugar fermenting cell
GB0822937D0 (en) 2008-12-16 2009-01-21 Terranol As Microorganism
FI20086236A0 (en) * 2008-12-23 2008-12-23 Valtion Teknillinen Conversion of hexuronic acid to hexaric acid
WO2010074577A1 (en) 2008-12-24 2010-07-01 Royal Nedalco B.V. Xylose isomerase genes and their use in fermentation of pentose sugars
FR2947233B1 (en) 2009-06-30 2013-02-15 Zf Systemes De Direction Nacam Sas ELECTRIC LOCKING SYSTEM OF A STEERING COLUMN AND STEERING COLUMN COMPRISING SUCH A LATCHING SYSTEM
US20120184007A1 (en) 2009-07-09 2012-07-19 Stephen Picataggio Engineered microorganisms with enhanced fermentation activity

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