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AR080636A1 - COMPOSITIONS AND METHODS TO MINIMIZE THE SYNTHESIS OF NORNICOTINE IN TOBACCO - Google Patents

COMPOSITIONS AND METHODS TO MINIMIZE THE SYNTHESIS OF NORNICOTINE IN TOBACCO

Info

Publication number
AR080636A1
AR080636A1 ARP110100113A ARP110100113A AR080636A1 AR 080636 A1 AR080636 A1 AR 080636A1 AR P110100113 A ARP110100113 A AR P110100113A AR P110100113 A ARP110100113 A AR P110100113A AR 080636 A1 AR080636 A1 AR 080636A1
Authority
AR
Argentina
Prior art keywords
tobacco
nornicotine
nicotine
methods
nicotine demethylase
Prior art date
Application number
ARP110100113A
Other languages
Spanish (es)
Original Assignee
Univ North Carolina State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina State filed Critical Univ North Carolina State
Publication of AR080636A1 publication Critical patent/AR080636A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pyridine Compounds (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Se proveen composiciones y métodos para reducir el nivel de nornicotina y N'-nitrosonornicotina (NNN) en plantas de tabaco y partes de las mismas. Las composiciones comprenden polinucleotidos y polipéptidos aislados para nicotinas demetilasas específicas para la raíz, CYP82E10, y variantes de las mismas, que intervienen en la conversion metabolica de nicotina en nornicotina en estas plantas. Las composiciones además incluyen plantas de tabaco, o partes de las mismas, que comprenden una mutacion en un gen que codifica una nicotina demetilasa CYP82E10, en tanto la mutacion da como resultado una expresion o funcion reducida de la nicotina demetilasa CYP82E 10. Además se proveen semillas de estas plantas de tabaco, o progenie de las mismas, y productos de tabaco preparados a partir de las plantas de tabaco de la solicitud, o de partes de las mismas o progenie de las mismas. Además se proveen métodos de reduccion del nivel de nornicotina, o de reduccion de la tasa de conversion de nicotina en nornicotina, en una planta de tabaco, o parte de la misma. Los métodos comprenden introducir en el genoma de una planta de tabaco una mutacion dentro de por lo menos un alelo de cada uno de por lo menos tres genes de nicotina demetilasa, en tanto la mutacion reduce la expresion del gen de nicotina demetilasa, y el primero de estos genes de nicotina demetilasa codifica una nicotina demetilasa específica de la raíz que interviene en la conversion metabolica de nicotina en nornicotina en una planta de tabaco o una parte de la misma. Los métodos son de aplicacion en la produccion de productos de tabaco que poseen niveles reducidos de nornicotina y su metabolito carcinogénico, NNN, y así un potencial carcinogénico reducido para aquellos individuos que consumen estos productos de tabaco o que están expuestos al humo secundario derivado de estos productos.Compositions and methods are provided to reduce the level of nornicotine and N'-nitrosonornicotine (NNN) in tobacco plants and parts thereof. The compositions comprise polynucleotides and isolated polypeptides for root-specific nicotine demethylases, CYP82E10, and variants thereof, which are involved in the metabolic conversion of nicotine to nornicotine in these plants. The compositions further include tobacco plants, or parts thereof, that comprise a mutation in a gene encoding a CYP82E10 nicotine demethylase, while the mutation results in a reduced expression or function of CYP82E 10 nicotine demethylase. In addition they are provided Seeds of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the application, or parts thereof or progeny thereof. In addition, methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine into nornicotine, in a tobacco plant, or part thereof, are provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, while the mutation reduces the expression of the nicotine demethylase gene, and the first of these nicotine demethylase genes encodes a specific root nicotine demethylase that is involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a part thereof. The methods are applicable in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus a reduced carcinogenic potential for those individuals who consume these tobacco products or who are exposed to secondary smoke derived from them. products.

ARP110100113A 2010-01-15 2011-01-13 COMPOSITIONS AND METHODS TO MINIMIZE THE SYNTHESIS OF NORNICOTINE IN TOBACCO AR080636A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29567110P 2010-01-15 2010-01-15

Publications (1)

Publication Number Publication Date
AR080636A1 true AR080636A1 (en) 2012-04-25

Family

ID=43722405

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP110100113A AR080636A1 (en) 2010-01-15 2011-01-13 COMPOSITIONS AND METHODS TO MINIMIZE THE SYNTHESIS OF NORNICOTINE IN TOBACCO
ARP200102910A AR120271A2 (en) 2010-01-15 2020-10-21 COMPOSITIONS AND METHODS TO MINIMIZE THE SYNTHESIS OF NORNICOTINE IN TOBACCO

Family Applications After (1)

Application Number Title Priority Date Filing Date
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Country Status (13)

Country Link
US (7) US9247706B2 (en)
EP (1) EP2524044B1 (en)
JP (1) JP6055311B2 (en)
KR (1) KR101611847B1 (en)
CN (1) CN102858983B (en)
AR (2) AR080636A1 (en)
AU (1) AU2011205282B2 (en)
CA (1) CA2786813C (en)
EA (1) EA033565B1 (en)
MX (1) MX337123B (en)
PH (2) PH12012501393B1 (en)
WO (1) WO2011088180A1 (en)
ZA (1) ZA201205426B (en)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
WO2006091194A1 (en) 2005-02-23 2006-08-31 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
WO2009064771A2 (en) 2007-11-12 2009-05-22 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
AU2011205282B2 (en) 2010-01-15 2014-10-02 North Carolina State University Compositions and methods for minimizing nornicotine synthesis in tobacco
JP2014515596A (en) 2011-02-28 2014-07-03 ノース カロライナ ステイト ユニヴァーシティ Tobacco inbred plants NCBEX1F, NCBEX1MS, and NCEX90
JP2014511184A (en) 2011-02-28 2014-05-15 アルトリア クライアント サービシーズ インコーポレイテッド Tobacco inbred plants ALBEX1F and ALBEX1MS
RU2735254C2 (en) 2012-12-21 2020-10-29 Филип Моррис Продактс С.А. Reduced tobacco-specific nitrosamines in plants
EP2943059A1 (en) 2013-01-11 2015-11-18 North Carolina State University Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src
EP2964016A1 (en) 2013-03-05 2016-01-13 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
US10375910B2 (en) 2013-03-15 2019-08-13 Altria Client Services Llc Development of tobacco varieties with no or significantly reduced anatabine content
US9710787B2 (en) * 2013-07-31 2017-07-18 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for representing, diagnosing, and recommending interaction sequences
CN105960460B (en) * 2013-12-06 2020-06-26 奥驰亚客户服务公司 Tobacco plants having altered amounts of one or more alkaloids in leaves and methods of using such plants
CN106659232A (en) * 2014-03-03 2017-05-10 北卡罗莱纳州立大学 Tobacco inbred and hybrid plants and tobacco products made thereof
WO2015134438A1 (en) * 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US9596823B2 (en) * 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
WO2015157359A1 (en) * 2014-04-08 2015-10-15 Altria Client Services Llc Tobacco having altered leaf properties and methods of making and using
US10415050B2 (en) 2014-05-08 2019-09-17 Philip Morris Products S.A. Reduction of nicotine to nornicotine conversion in plants
MX2017003932A (en) * 2014-09-26 2017-06-30 Philip Morris Products Sa Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway.
WO2016057515A2 (en) 2014-10-06 2016-04-14 Altria Client Services Llc Genetic control of axillary bud growth in tobacco plants
JP2018516550A (en) * 2015-05-05 2018-06-28 ノース カロライナ ステート ユニバーシティＮｏｒｔｈ Ｃａｒｏｌｉｎａ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ Methods and compositions for reducing tobacco-specific nitrosamine NNK in tobacco
EP4218404A3 (en) * 2015-06-26 2023-09-06 Altria Client Services LLC Compositions and methods for producing tobacco plants and products having altered alkaloid levels
CN105602961B (en) * 2015-09-07 2018-10-23 中国农业科学院烟草研究所 The flue-cured tobacco CYP82E4 genes of mutation and its application
EA201891707A1 (en) 2016-01-29 2018-12-28 Филип Моррис Продактс С.А. DECREASE IN ACCUMULATION OF CADMIUM IN TOBACCO PLANTS GROWN IN THE FIELD
CN117089526A (en) 2016-03-11 2023-11-21 奥驰亚客户服务有限公司 Compositions and methods for producing tobacco plants and articles with reduced or eliminated bifurcations
EP3523439A1 (en) 2016-10-07 2019-08-14 Altria Client Services LLC Composition and methods for producing tobacco plants and products having reduced tobacco-specific nitrosamines (tsnas)
AU2017380893A1 (en) 2016-12-20 2019-05-02 Philip Morris Products S.A. Plants with shortened time to flowering
WO2018119124A1 (en) 2016-12-21 2018-06-28 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels
CN106636146B (en) * 2017-02-16 2019-09-13 云南省烟草农业科学研究院 It is a kind of reduce nicotine conversion ratio CYP82E5 gene mutation body and its application
US11104912B2 (en) 2017-09-11 2021-08-31 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having reduced or eliminated suckers
EP3480314A1 (en) * 2017-11-03 2019-05-08 Philip Morris Products S.A. Regulation of alkaloid content
BR112020011386A2 (en) 2017-12-07 2020-12-01 R.J. Reynolds Tobacco Company bzip transcription factors regulate the conversion of nicotine to nornicotine
CN111836539A (en) * 2018-01-12 2020-10-27 奥驰亚客户服务有限公司 Compositions and methods for producing tobacco plants and products with altered alkaloid levels
CN108192900B (en) * 2018-01-16 2021-11-30 西南大学 Editing inactivation vector of tobacco nicotine demethylase gene CYP82E4 and application thereof
JP2021516055A (en) 2018-03-05 2021-07-01 アルトリア クライアント サーヴィシーズ リミテッド ライアビリティ カンパニー Compositions and Methods for Producing Tobacco Plants and Tobacco Products With Altered Alkaloid Levels and Desired Leaf Quality
BR112020017430A2 (en) 2018-03-28 2021-01-19 Philip Morris Products S.A. MODULATION OF REDUCING SUGAR CONTENT IN A PLANT
BR112020017500A2 (en) 2018-03-28 2020-12-22 Philip Morris Products S.A. MODULATION OF AMINO ACIDS CONTENT IN A PLANT
US11377665B2 (en) 2018-04-03 2022-07-05 Altria Client Services Llc Composition and methods for producing tobacco plants and products having increased phenylalanine and reduced tobacco-specific nitrosamines (TSNAs)
CN108424922B (en) * 2018-04-20 2021-04-16 云南省烟草农业科学研究院 CYP82E10 gene missense mutant M271 capable of reducing nicotine conversion rate and application thereof
BR112021001364A2 (en) 2018-07-26 2021-04-20 Altria Client Services Llc pmt engineering-based compositions and methods for producing tobacco plants and products having altered alkaloid levels
WO2020141062A1 (en) 2018-12-30 2020-07-09 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
EP3914069A1 (en) 2019-01-24 2021-12-01 Altria Client Services LLC Tobacco plants comprising reduced nicotine and reduced tobacco specific nitrosamines
BR112021024528A2 (en) 2019-06-05 2022-01-18 Reynolds Tobacco Co R bzip transcription factors regulate the conversion of nicotine to nornicotine and reduce levels of tobacco-specific precursors (tsna)
EP4041753A1 (en) 2019-10-01 2022-08-17 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (sultr3)
EP4041896A1 (en) 2019-10-01 2022-08-17 Philip Morris Products S.A. Modulating reducing sugar content in a plant (inv)
EP4041898A1 (en) 2019-10-10 2022-08-17 Altria Client Services LLC Compositions and methods for producing tobacco plants and products having altered alkaloid levels with desirable leaf quality via manipulating leaf quality genes
JP2022552261A (en) 2019-10-10 2022-12-15 アルトリア クライアント サーヴィシーズ リミテッド ライアビリティ カンパニー Compositions and Methods Based on QPT Engineering for Making Tobacco Plants and Tobacco Products with Altered Alkaloid Levels
US20240218386A1 (en) 2019-12-03 2024-07-04 Altria Client Services Llc Compositions and Methods for Producing Tobacco Plants and Products Having Altered Alkaloid Levels
CN115516098A (en) 2020-01-17 2022-12-23 奥驰亚客户服务有限公司 Methods and compositions related to improved nitrogen use efficiency
WO2021154738A1 (en) 2020-01-27 2021-08-05 Altria Client Services Llc Compositions and methods based on pmt engineering for producing tobacco plants and products having altered alkaloid levels
US11536685B2 (en) 2020-02-06 2022-12-27 Trustees Of Boston University High throughput assay for identifying microbial redox enzymes
WO2021158973A1 (en) 2020-02-06 2021-08-12 Trustees Of Boston University Enzyme-based electrochemical nicotine biosensor
WO2021236791A1 (en) 2020-05-20 2021-11-25 Altria Client Services Llc A hybrid air and fire curing combination process to reduce harmful and potentially harmful constituents in cured tobacco
CN111662912A (en) * 2020-06-01 2020-09-15 云南省烟草农业科学研究院 Tobacco NtARF6 gene mutant and molecular identification method and application
CN119138333A (en) 2020-06-03 2024-12-17 奥驰亚客户服务有限公司 Compositions and methods for producing tobacco plants and products having altered alkaloid levels
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
WO2023230433A1 (en) 2022-05-23 2023-11-30 Altria Client Services Llc Methods and compositions for regulating alkaloids in tobacco field
CN119948047A (en) 2022-08-05 2025-05-06 奥驰亚客户服务有限公司 Methods and compositions for regulating alkaloids in tobacco
KR20250078994A (en) 2022-10-13 2025-06-04 필립모리스 프로덕츠 에스.에이. Increased leaf biomass and nitrogen use efficiency by NTP2 regulation
CN120530195A (en) 2023-02-02 2025-08-22 菲利普莫里斯生产公司 Regulation of sugar transporters
EP4658670A1 (en) 2023-02-02 2025-12-10 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
AU2024303302A1 (en) * 2023-06-16 2026-01-08 North Carolina State University Tobacco plants with reduced nicotinic alkaloid levels
CN121335615A (en) 2023-06-29 2026-01-13 菲利普莫里斯生产公司 Modulation of genes encoding glutamate dehydrogenase
WO2025080632A1 (en) 2023-10-11 2025-04-17 Altria Client Services Llc Compositions and methods to alter alkaloids in tobacco
WO2025147663A2 (en) 2024-01-05 2025-07-10 Altria Client Services Llc Methods and compositions for modulating trichome density and flavor molecule secretion

Family Cites Families (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
EP0320500B1 (en) 1983-01-13 2004-11-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Non-oncogenic ti plasmid vector system and recombinant DNA molecules for the introduction of expressible genes into plant cell genomes
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
WO1984002919A1 (en) 1983-01-17 1984-08-02 Monsanto Co Plasmids for transforming plant cells
NL8300698A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; AGROBACTERIUM TUMEFACIENS BACTERIA AND METHOD FOR PRODUCTION THEREOF; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
NL8300699A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; METHOD FOR PRODUCING AGROBACTERIUM TUMEFACIENS BACTERIEN; STABLE COINTEGRATE PLASMIDS; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
US4732856A (en) 1984-04-03 1988-03-22 Carnegie Institution Of Washington Transposable elements and process for using same
NL8401048A (en) 1984-04-03 1985-11-01 Rijksuniversiteit Leiden En Pr METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF LOBELIC PLANTS.
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
NL8401780A (en) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr METHOD FOR BUILDING FOREIGN DNA INTO THE GENOMES OF PLANTS
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
AU7360087A (en) 1986-04-30 1987-11-24 Boyce Thompson Institute For Plant Research Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
GB8615676D0 (en) * 1986-06-26 1986-07-30 Stoppers Co Ltd Nicotine containing lozenge
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US4801540A (en) 1986-10-17 1989-01-31 Calgene, Inc. PG gene and its use in plants
EP0267159A3 (en) 1986-11-07 1990-05-02 Ciba-Geigy Ag Process for the genetic modification of monocotyledonous plants
SE455438B (en) 1986-11-24 1988-07-11 Aga Ab SET TO REDUCE A BURNER'S FLAME TEMPERATURE AND BURNER WITH THE OXYGEN RESP FUEL NOZZLE
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5350689A (en) 1987-05-20 1994-09-27 Ciba-Geigy Corporation Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
ES2074999T3 (en) 1987-05-20 1995-10-01 Ciba Geigy Ag ZEA MAYS PLANTS AND TRANSGENIC PLANTS REGENERATED FROM PROTOPLASTS OR CELLS DERIVED FROM PROTOPLASTS.
US5013658A (en) 1988-05-13 1991-05-07 Dna Plant Technology Corporation Transposon tagging of genes in transformed plants
CA1339684C (en) 1988-05-17 1998-02-24 Peter H. Quail Plant ubquitin promoter system
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5141131A (en) 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
EP0426641B1 (en) 1989-10-31 2000-09-13 Monsanto Company Promoter for transgenic plants
US5668295A (en) 1990-11-14 1997-09-16 Philip Morris Incorporated Protein involved in nicotine synthesis, DNA encoding, and use of sense and antisense DNAs corresponding thereto to affect nicotine content in transgenic tobacco cells and plants
US5260205A (en) 1990-11-14 1993-11-09 Philip Morris Incorporated Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine
DE69133512T2 (en) 1990-11-23 2006-09-28 Bayer Bioscience N.V. Process for the transformation of monocotyledonous plants
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
CA2130454C (en) 1992-02-19 2008-04-08 William G. Dougherty Production of viral resistant plants via introduction of untranslatable plus sense viral rna
JPH07505531A (en) 1992-04-15 1995-06-22 プラント・ジェネティック・システムズ・エヌ・ブイ Transformation method for monocot cells
US5311143A (en) 1992-07-02 1994-05-10 Motorola, Inc. RF amplifier bias control method and apparatus
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
ES2105415T3 (en) 1993-06-04 1997-10-16 Cavis Srl INERTIA DEVICE TO INTERRUPT THE ELECTRICAL CIRCUIT OF A VEHICLE WITH AN INTERNAL COMBUSTION ENGINE.
DE69637875D1 (en) 1995-09-14 2009-04-30 Virginia Tech Intell Prop Production of lysosomal enzymes in plant expression systems
US5713376A (en) 1996-05-13 1998-02-03 Berger; Carl Non-addictive tobacco products
US7238860B2 (en) 2001-04-18 2007-07-03 Mendel Biotechnology, Inc. Yield-related polynucleotides and polypeptides in plants
EP1033405A3 (en) 1999-02-25 2001-08-01 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
AU4991500A (en) 1999-05-06 2000-11-21 Michael Timko Regulation of gene expression in tobacco for manipulation of plant growth and secondary metabolism
AU2001257300A1 (en) 2000-04-26 2001-11-07 Monsanto Technology Llc Method for the transformation of plant cell plastids
CA2954889A1 (en) 2001-03-09 2002-09-19 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
US20060157072A1 (en) 2001-06-08 2006-07-20 Anthony Albino Method of reducing the harmful effects of orally or transdermally delivered nicotine
MXPA03011385A (en) 2001-06-08 2005-03-07 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco.
US6953040B2 (en) 2001-09-28 2005-10-11 U.S. Smokeless Tobacco Company Tobacco mint plant material product
US7032601B2 (en) 2001-09-28 2006-04-25 U.S. Smokeless Tobacco Company Encapsulated materials
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US7700851B2 (en) 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US20040103449A1 (en) 2001-11-13 2004-05-27 U.S. Smokeless Tobacco Company Identification and use of cytochrome P450 nucleic acid sequences from tobacco
US20040111759A1 (en) 2001-11-13 2004-06-10 U.S. Smokeless Tobacco Company Identification and use of cytochrome P450 nucleic acid sequences from tobacco
US20040117869A1 (en) 2002-01-11 2004-06-17 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US20040162420A1 (en) 2002-10-16 2004-08-19 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
EP1572911A4 (en) 2002-03-12 2008-06-25 Us Smokeless Tobacco Co Cloning of cytochrome p450 genes from nicotiana
AP2005003286A0 (en) 2002-10-16 2005-06-30 Us Smokeless Tobacco Co Cloning of cytochrome p450 genes from nicotiana
AU2004282576B2 (en) 2002-10-16 2010-01-28 U S Smokeless Tobacco Company Llc Cloning of cytochrome P450 genes from nicotiana
US20050019448A1 (en) 2003-07-22 2005-01-27 Kevin Engelhardt Liquid flavoring dissolving strips and method of use thereof
US8637731B2 (en) 2003-10-16 2014-01-28 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
EP1694832B1 (en) 2003-10-16 2012-04-18 U.S. Smokeless Tobacco Company LLC Use of a cytochrome p450 gene from nicotiana
ZA200602938B (en) 2003-10-16 2008-06-25 Us Smokeless Tobacco Company Cloning of cytochrome p450 genes from Nicotiana
WO2005046363A2 (en) 2003-11-07 2005-05-26 U.S. Smokeless Tobacco Company Tobacco compositions
US8627828B2 (en) 2003-11-07 2014-01-14 U.S. Smokeless Tobacco Company Llc Tobacco compositions
TW200531647A (en) 2003-12-22 2005-10-01 Us Smokeless Tobacco Co Conditioning process for tobacco and/or snuff compositions
US8586837B2 (en) * 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
PL2295549T3 (en) * 2004-04-29 2015-10-30 U S Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
EP1781123A1 (en) 2004-08-23 2007-05-09 U.S. Smokeless Tobacco Company Nicotiana compositions
US20060185686A1 (en) 2004-08-23 2006-08-24 Lawrence Robert H Jr Nicotiana diversity
US20070199097A1 (en) 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
WO2006091194A1 (en) * 2005-02-23 2006-08-31 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
EA012674B1 (en) 2005-04-29 2009-12-30 Филип Моррис Продактс С.А. Tobacco pouch product
US7757697B2 (en) 2005-12-22 2010-07-20 Swisher International, Inc. Method for reducing nitrosamines in tobacco
EP2087123B1 (en) 2006-10-13 2011-08-10 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US8319011B2 (en) * 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8186360B2 (en) 2007-04-04 2012-05-29 R.J. Reynolds Tobacco Company Cigarette comprising dark air-cured tobacco
US7667104B2 (en) 2007-11-06 2010-02-23 Alliance One International, Inc. Tobacco cultivar AOB 171
WO2009064771A2 (en) * 2007-11-12 2009-05-22 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
AU2011205282B2 (en) * 2010-01-15 2014-10-02 North Carolina State University Compositions and methods for minimizing nornicotine synthesis in tobacco
JP5550433B2 (en) 2010-04-22 2014-07-16 任天堂株式会社 Operating device and information processing system
JP2014515596A (en) 2011-02-28 2014-07-03 ノース カロライナ ステイト ユニヴァーシティ Tobacco inbred plants NCBEX1F, NCBEX1MS, and NCEX90
EP2943059A1 (en) 2013-01-11 2015-11-18 North Carolina State University Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src
EP2964016A1 (en) 2013-03-05 2016-01-13 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
US9596823B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
CN106659232A (en) 2014-03-03 2017-05-10 北卡罗莱纳州立大学 Tobacco inbred and hybrid plants and tobacco products made thereof
US9560823B2 (en) 2015-05-01 2017-02-07 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH103948
US9560822B2 (en) 2015-05-01 2017-02-07 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH100298
US9560824B2 (en) 2015-05-01 2017-02-07 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH138178

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