AR059267A1 - BACTERIA PHOSPHOPANTETININ TRANSFERS - Google Patents
BACTERIA PHOSPHOPANTETININ TRANSFERSInfo
- Publication number
- AR059267A1 AR059267A1 ARP070100414A AR059267A1 AR 059267 A1 AR059267 A1 AR 059267A1 AR P070100414 A ARP070100414 A AR P070100414A AR 059267 A1 AR059267 A1 AR 059267A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- phosphopanteteinyl
- polynucleotide
- polypeptide
- acid
- Prior art date
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente se refiere en general a fosfopanteteinil transferasas que se requieren para la activacion de un complejo de policétido sintasa para sintetizar ácidos grasos poliinsaturados de cadena larga (LC-PUFA) tales como ácido docosahexenoico o ácido eicosapentenoico. En particular, la presente se refiere a fosfopanteteinil transferasas bacterianas, a construcciones de ADN para su expresion en células hospedantes y a semillas, aceite y harina cuando las células hospedantes comprenden una planta. También se proporciona un método para preparar un aceite de planta que contiene ácido docosahexenoico o ácido eicosapentenoico. Reivindicacion 1: Un polinucleotido aislado que comprende una secuencia seleccionada del grupo que consiste en: (a) un polinucleotido que hibridiza a la SEC ID N°:6 o SEC ID N°:8, o un complemento de la misma, en condiciones de SSC 5X, formamida 50% y 42°C, donde el polinucleotido codifica el polipéptido con actividad de fosfopanteteinil transferasa; (b) un polinucleotido que codifica la secuencia polipeptídica de SEC ID N°:5 o SEC ID N°:7, y (c) un polinucleotido que codifica un polipéptido con actividad de fosfopanteteinil transferasa y que tiene por lo menos 75% de identidad de secuencia con una secuencia polipeptídica de SEC ID N°:5 o SEC ID N°:7.This refers in general to phosphopanteteinyl transferases that are required for the activation of a polyketide synthase complex to synthesize long chain polyunsaturated fatty acids (LC-PUFA) such as docosahexenoic acid or eicosapentenoic acid. In particular, this refers to bacterial phosphopanteteinyl transferases, to DNA constructs for expression in host cells and to seeds, oil and flour when the host cells comprise a plant. A method for preparing a plant oil containing docosahexenoic acid or eicosapentenoic acid is also provided. Claim 1: An isolated polynucleotide comprising a sequence selected from the group consisting of: (a) a polynucleotide that hybridizes to SEQ ID NO: 6 or SEQ ID NO: 8, or a complement thereof, under conditions of 5X SSC, 50% formamide and 42 ° C, where the polynucleotide encodes the polypeptide with phosphopanteteinyl transferase activity; (b) a polynucleotide encoding the polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, and (c) a polynucleotide encoding a polypeptide with phosphopanteteinyl transferase activity and having at least 75% identity sequence with a polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US76364406P | 2006-01-31 | 2006-01-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR059267A1 true AR059267A1 (en) | 2008-03-19 |
Family
ID=39272757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP070100414 AR059267A1 (en) | 2006-01-31 | 2007-01-31 | BACTERIA PHOSPHOPANTETININ TRANSFERS |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN101415822B (en) |
| AR (1) | AR059267A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112540077B (en) * | 2020-11-30 | 2022-12-06 | 邢台学院 | A method for in situ detection of fatty acid unsaturation in seeds |
| CN114689704B (en) * | 2020-12-26 | 2023-05-09 | 四川汇宇制药股份有限公司 | Detection method of 1,3-dihydroxyacetone and related impurities |
-
2007
- 2007-01-30 CN CN200780012056.7A patent/CN101415822B/en active Active
- 2007-01-31 AR ARP070100414 patent/AR059267A1/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CN101415822A (en) | 2009-04-22 |
| CN101415822B (en) | 2016-06-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |