AR038894A1 - Procedimiento de produccion de semillas hibridas de maiz - Google Patents
Procedimiento de produccion de semillas hibridas de maizInfo
- Publication number
- AR038894A1 AR038894A1 ARP030100785A ARP030100785A AR038894A1 AR 038894 A1 AR038894 A1 AR 038894A1 AR P030100785 A ARP030100785 A AR P030100785A AR P030100785 A ARP030100785 A AR P030100785A AR 038894 A1 AR038894 A1 AR 038894A1
- Authority
- AR
- Argentina
- Prior art keywords
- corn
- small grain
- transgene
- ams
- seeds
- Prior art date
Links
- 240000008042 Zea mays Species 0.000 title abstract 16
- 235000002017 Zea mays subsp mays Nutrition 0.000 title abstract 16
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title abstract 14
- 235000005822 corn Nutrition 0.000 title abstract 14
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 235000013339 cereals Nutrition 0.000 abstract 11
- 239000003550 marker Substances 0.000 abstract 10
- 108700019146 Transgenes Proteins 0.000 abstract 9
- 230000035558 fertility Effects 0.000 abstract 9
- 108090000623 proteins and genes Proteins 0.000 abstract 9
- 206010021929 Infertility male Diseases 0.000 abstract 3
- 208000007466 Male Infertility Diseases 0.000 abstract 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 2
- 235000009973 maize Nutrition 0.000 abstract 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 241001147665 Foraminifera Species 0.000 abstract 1
- 101000708283 Oryza sativa subsp. indica Protein Rf1, mitochondrial Proteins 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 239000003337 fertilizer Substances 0.000 abstract 1
- 238000003205 genotyping method Methods 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Un procedimiento de producción y de multiplicación de plántulas de maíz homocigotas para un transgen que confiere esterilidad masculina, útiles para la producción de semillas híbridas de maíz. Reivindicación 1: Procedimiento de producción de semillas de maíz homocigotas para un transgen que confiere esterilidad masculina nuclear artificial ("AMS") y heterocigotas para un gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno", caracterizado porque comprende las etapas que consisten en: a) cruzar una plántula de maíz masculina estéril heterocigota para el transgen AMS con una plántula de maíz restauradora de la fertilidad que comprende en su genoma un gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno", b) seleccionar por el fenotipo "grano pequeno" las semillas de maíz que comprenden en su genoma un gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno", c) autofecundar las plántulas de maíz resultantes de las semillas seleccionadas de acuerdo con la etapa (b), d) seleccionar las semillas homocigotas para el transgen AMS y heterocigotas para el gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno". Reivindicación 2: Procedimiento de producción de semillas de maíz homocigotas para un transgen que confiere esterilidad masculina nuclear artificial ("AMS") y heterocigotas para un gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno", caracterizado porque comprende las etapas que consisten en: a) cruzar una plántula de maíz masculina estéril heterocigota para el transgen AMS con una plántula de maíz restaurador de la fertilidad que comprende en su genoma un gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno", b) realizar un genotipado de las semillas obtenidas por el cruzamiento de acuerdo con la etapa (a), c) autofecundar las plántulas de maíz resultantes de las semillas genotipeadas de acuerdo con la etapa (b), d) seleccionar las semillas homocigotas para el transgen AMS y heterocigotas para el gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno". Reivindicación 3: Semilla de maíz homocigota para un transgen AMS y heterocigota para un gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno", caracterizado porque es susceptible de obtenerse por el procedimiento de acuerdo con la reivindicación 1 o 2. Reivindicación 20: Vector, en especial plásmido, caracterizado porque contiene al menos una casete de expresión tal como se ha descripto en una de las reivindicaciones 11 a 19. Reivindicación 21: Huésped celular, caracterizado porque es en especial bacteria tal como Agrobacterium tumefaciens, transformado por un vector de acuerdo con la reivindicación 20. Reivindicación 22: Célula de maíz caracterizada porque es transformada por al menos un vector de acuerdo con la reivindicación 20. Reivindicación 23: Plántula de maíz restaurador de la fertilidad caracterizada porque comprende en su genoma un gen restaurador de la fertilidad ligado a un marcador de fenotipo "grano pequeno". Reivindicación 24: Plántula de maíz homocigota para un transgen AMS y heterocigota para un gen de restauración de la fertilidad ligado a un marcador de fenotipo "grano pequeno", caracterizada porque se obtiene a partir de una semilla de acuerdo con la reivindicación 3.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0202968A FR2836782B1 (fr) | 2002-03-08 | 2002-03-08 | Nouveau procede de production de semences hybrides de mais |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR038894A1 true AR038894A1 (es) | 2005-02-02 |
Family
ID=27763666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030100785A AR038894A1 (es) | 2002-03-08 | 2003-03-07 | Procedimiento de produccion de semillas hibridas de maiz |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20050120416A1 (es) |
| EP (1) | EP1485493A1 (es) |
| AR (1) | AR038894A1 (es) |
| AU (1) | AU2003227816A1 (es) |
| CA (1) | CA2478126A1 (es) |
| FR (1) | FR2836782B1 (es) |
| WO (1) | WO2003076632A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7517975B2 (en) * | 2000-09-26 | 2009-04-14 | Pioneer Hi-Bred International, Inc. | Nucleotide sequences mediating male fertility and method of using same |
| US7612251B2 (en) | 2000-09-26 | 2009-11-03 | Pioneer Hi-Bred International, Inc. | Nucleotide sequences mediating male fertility and method of using same |
| EP2333075B1 (en) | 2003-12-16 | 2018-07-04 | Pioneer Hi-Bred International Inc. | Dominant gene suppression transgenes and methods of using same |
| US20080244765A1 (en) * | 2004-12-16 | 2008-10-02 | Pioneer Hi-Bred International, Inc. | Methods and compositions for pollination disruption |
| US7910802B2 (en) * | 2007-08-03 | 2011-03-22 | Pioneer Hi-Bred International, Inc. | MSCA1 nucleotide sequences impacting plant male fertility and method of using same |
| US7915478B2 (en) | 2007-08-03 | 2011-03-29 | Pioneer Hi-Bred International, Inc. | Msca1 nucleotide sequences impacting plant male fertility and method of using same |
| US7919676B2 (en) * | 2007-08-03 | 2011-04-05 | Pioneer Hi-Bred International, Inc. | Msca1 nucleotide sequences impacting plant male fertility and method of using same |
| EP2257076B1 (en) * | 2009-05-28 | 2015-02-25 | Advanced Digital Broadcast S.A. | Video data signal, system and method for controlling shutter glasses |
| CN105018475B (zh) * | 2015-06-03 | 2017-02-22 | 北京首佳利华科技有限公司 | 基于Ms1基因构建的介导玉米雄性生育力的多控不育载体及其应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9028060D0 (en) * | 1990-12-24 | 1991-02-13 | Nickerson Int Seed | Recombinant dna |
| CA2191441A1 (en) * | 1994-06-06 | 1995-12-21 | Enno Krebbers | Use of anthocyanin genes to maintain male sterile plants |
| GB9412018D0 (en) * | 1994-06-16 | 1994-08-03 | Cambridge Advanced Tech | Modification of starch content in plants |
| US5962769A (en) * | 1995-06-07 | 1999-10-05 | Pioneer Hi-Bred International, Inc. | Induction of male sterility in plants by expression of high levels of avidin |
-
2002
- 2002-03-08 FR FR0202968A patent/FR2836782B1/fr not_active Expired - Fee Related
-
2003
- 2003-03-05 WO PCT/FR2003/000711 patent/WO2003076632A1/fr not_active Application Discontinuation
- 2003-03-05 CA CA002478126A patent/CA2478126A1/fr not_active Abandoned
- 2003-03-05 US US10/506,946 patent/US20050120416A1/en not_active Abandoned
- 2003-03-05 EP EP03725269A patent/EP1485493A1/fr not_active Withdrawn
- 2003-03-05 AU AU2003227816A patent/AU2003227816A1/en not_active Abandoned
- 2003-03-07 AR ARP030100785A patent/AR038894A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20050120416A1 (en) | 2005-06-02 |
| FR2836782B1 (fr) | 2004-06-04 |
| CA2478126A1 (fr) | 2003-09-18 |
| EP1485493A1 (fr) | 2004-12-15 |
| AU2003227816A1 (en) | 2003-09-22 |
| WO2003076632A1 (fr) | 2003-09-18 |
| FR2836782A1 (fr) | 2003-09-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |