AR012499A1 - Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin - Google Patents
Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombinInfo
- Publication number
- AR012499A1 AR012499A1 ARP980101873A ARP980101873A AR012499A1 AR 012499 A1 AR012499 A1 AR 012499A1 AR P980101873 A ARP980101873 A AR P980101873A AR P980101873 A ARP980101873 A AR P980101873A AR 012499 A1 AR012499 A1 AR 012499A1
- Authority
- AR
- Argentina
- Prior art keywords
- fusion protein
- vector
- chemical sequence
- repared
- vito
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Plant Pathology (AREA)
- Nutrition Science (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Toxicology (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se describe un método mejorado para recuperar polipéptidos producidos de manera recombinante. El método incluye expresar el polipéptido recombinantecomo una proteína de fusion con un pro-péptido. La proteína de fusion pro-péptido-polipép tido puede clivarse y el polipéptido recombinante puede serliberado bajo condiciones apropiadas
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4425497P | 1997-04-25 | 1997-04-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR012499A1 true AR012499A1 (es) | 2000-10-18 |
Family
ID=21931348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP980101873A AR012499A1 (es) | 1997-04-25 | 1998-04-23 | Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US7501265B1 (es) |
| EP (1) | EP0977873B1 (es) |
| JP (1) | JP4120972B2 (es) |
| KR (2) | KR100609886B1 (es) |
| AR (1) | AR012499A1 (es) |
| AT (1) | ATE341633T1 (es) |
| AU (1) | AU742794B2 (es) |
| BR (1) | BR9809416A (es) |
| CA (1) | CA2286861A1 (es) |
| DE (1) | DE69836075T2 (es) |
| DK (1) | DK0977873T3 (es) |
| ES (1) | ES2273414T3 (es) |
| IL (2) | IL132259A0 (es) |
| MX (1) | MX219629B (es) |
| NZ (1) | NZ500736A (es) |
| TW (1) | TW505697B (es) |
| WO (1) | WO1998049326A1 (es) |
| ZA (1) | ZA983471B (es) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003531567A (ja) * | 1999-04-30 | 2003-10-28 | オーソ−マクニール・フアーマシユーチカル・インコーポレーテツド | チモーゲン活性化系 |
| US6420157B1 (en) * | 1999-04-30 | 2002-07-16 | Ortho-Mcneil Pharmaceutical, Inc. | Zymogen activation system |
| ATE335826T1 (de) * | 1999-08-09 | 2006-09-15 | Sandoz Ag | Proteinherstellung |
| AU775681B2 (en) * | 1999-08-09 | 2004-08-12 | Sandoz Ag | Production of proteins by autoproteolytic cleavage |
| MXPA02001904A (es) * | 1999-08-23 | 2002-10-31 | Sembiosys Genetics Inc | Produccion comercial de quimosina en plantas. |
| US7390936B1 (en) | 1999-08-23 | 2008-06-24 | Sembiosys Genetics Inc. | Commercial production of chymosin in plants |
| US6750034B1 (en) | 2000-06-30 | 2004-06-15 | Ortho-Mcneil Pharmaceutical, Inc. | DNA encoding human serine protease D-G |
| US6737262B1 (en) | 2000-07-11 | 2004-05-18 | Robert I. Bolla | Animal feed containing polypeptides |
| US7547821B2 (en) | 2003-06-17 | 2009-06-16 | Sembiosys Genetics Inc. | Methods for the production of insulin in plants |
| US7824885B2 (en) * | 2003-08-06 | 2010-11-02 | University Of Maryland, College Park | Engineered proteases for affinity purification and processing of fusion proteins |
| CN102389575B (zh) * | 2011-11-18 | 2013-01-23 | 武汉凯肽来生物科技有限公司 | 一种基因工程口服dna疫苗及制备方法和应用 |
| CN104619726B (zh) * | 2012-03-23 | 2018-05-18 | 苏州鲲鹏生物技术有限公司 | 由超折叠绿色荧光蛋白构成的融合蛋白及其用途 |
| US10894812B1 (en) | 2020-09-30 | 2021-01-19 | Alpine Roads, Inc. | Recombinant milk proteins |
| US10947552B1 (en) | 2020-09-30 | 2021-03-16 | Alpine Roads, Inc. | Recombinant fusion proteins for producing milk proteins in plants |
| AU2021353004A1 (en) | 2020-09-30 | 2023-04-13 | Nobell Foods, Inc. | Recombinant milk proteins and food compositions comprising the same |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4180559A (en) * | 1978-01-05 | 1979-12-25 | Richardson-Merrell Inc. | Coated 1-(2-chlorodibenzo[b,f]oxepin-10-yl)-4-methylpiperazine compositions |
| US4774183A (en) * | 1983-06-29 | 1988-09-27 | Kansas State University Research Foundation | Process for producing fructose |
| AU3036684A (en) | 1983-07-07 | 1985-01-10 | Genex Corp. | Production of bovine calf chymosin |
| US4743679A (en) | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
| JP3188264B2 (ja) | 1990-01-24 | 2001-07-16 | ファルマシア・アンド・アップジョン・カンパニー | 組換えポリペプチド類の精製法 |
| US5650554A (en) * | 1991-02-22 | 1997-07-22 | Sembiosys Genetics Inc. | Oil-body proteins as carriers of high-value peptides in plants |
| ATE242330T1 (de) * | 1993-01-15 | 2003-06-15 | Inst Genetics Llc | Klonierung von enterokinase und verfahren zu deren verwendung |
| US6265204B1 (en) * | 1997-01-17 | 2001-07-24 | Genencor International, Inc. | DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi |
-
1998
- 1998-04-23 DK DK98916746T patent/DK0977873T3/da active
- 1998-04-23 DE DE69836075T patent/DE69836075T2/de not_active Expired - Fee Related
- 1998-04-23 JP JP54643998A patent/JP4120972B2/ja not_active Expired - Fee Related
- 1998-04-23 AT AT98916746T patent/ATE341633T1/de not_active IP Right Cessation
- 1998-04-23 KR KR1019997009596A patent/KR100609886B1/ko not_active IP Right Cessation
- 1998-04-23 ES ES98916746T patent/ES2273414T3/es not_active Expired - Lifetime
- 1998-04-23 AR ARP980101873A patent/AR012499A1/es active IP Right Grant
- 1998-04-23 KR KR1020057018518A patent/KR100635623B1/ko not_active IP Right Cessation
- 1998-04-23 AU AU70240/98A patent/AU742794B2/en not_active Ceased
- 1998-04-23 TW TW087106216A patent/TW505697B/zh active
- 1998-04-23 BR BR9809416-5A patent/BR9809416A/pt not_active IP Right Cessation
- 1998-04-23 US US09/402,488 patent/US7501265B1/en not_active Expired - Fee Related
- 1998-04-23 CA CA002286861A patent/CA2286861A1/en not_active Abandoned
- 1998-04-23 IL IL13225998A patent/IL132259A0/xx active IP Right Grant
- 1998-04-23 EP EP98916746A patent/EP0977873B1/en not_active Expired - Lifetime
- 1998-04-23 NZ NZ500736A patent/NZ500736A/en unknown
- 1998-04-23 WO PCT/CA1998/000398 patent/WO1998049326A1/en active IP Right Grant
- 1998-04-23 MX MX9909801A patent/MX219629B/es not_active IP Right Cessation
- 1998-04-24 ZA ZA983471A patent/ZA983471B/xx unknown
-
1999
- 1999-10-07 IL IL132259A patent/IL132259A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU742794B2 (en) | 2002-01-10 |
| KR100635623B1 (ko) | 2006-10-18 |
| CA2286861A1 (en) | 1998-11-05 |
| ATE341633T1 (de) | 2006-10-15 |
| NZ500736A (en) | 2005-01-28 |
| US7501265B1 (en) | 2009-03-10 |
| ES2273414T3 (es) | 2007-05-01 |
| EP0977873A1 (en) | 2000-02-09 |
| MX9909801A (es) | 2000-03-31 |
| DK0977873T3 (da) | 2007-02-05 |
| EP0977873B1 (en) | 2006-10-04 |
| IL132259A0 (en) | 2001-03-19 |
| WO1998049326A1 (en) | 1998-11-05 |
| JP4120972B2 (ja) | 2008-07-16 |
| KR20050100712A (ko) | 2005-10-19 |
| ZA983471B (en) | 1998-10-27 |
| DE69836075D1 (de) | 2006-11-16 |
| DE69836075T2 (de) | 2007-06-06 |
| AU7024098A (en) | 1998-11-24 |
| JP2001527400A (ja) | 2001-12-25 |
| KR20010006506A (ko) | 2001-01-26 |
| KR100609886B1 (ko) | 2006-08-18 |
| TW505697B (en) | 2002-10-11 |
| MX219629B (es) | 2004-03-30 |
| BR9809416A (pt) | 2000-06-13 |
| IL132259A (en) | 2007-05-15 |
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Legal Events
| Date | Code | Title | Description |
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| FG | Grant, registration |