AR008363A1 - RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCING CELL LINE AND RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCED BY THIS CELL. - Google Patents
RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCING CELL LINE AND RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCED BY THIS CELL.Info
- Publication number
- AR008363A1 AR008363A1 ARP990100679A AR008363A1 AR 008363 A1 AR008363 A1 AR 008363A1 AR P990100679 A ARP990100679 A AR P990100679A AR 008363 A1 AR008363 A1 AR 008363A1
- Authority
- AR
- Argentina
- Prior art keywords
- erythropoyetine
- recombinant human
- gene
- epo
- produced
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
El gen codificante para eritropoyetina humana (EPO) se obtuvo a partir de ADN genomico humano. El gen utilizado no incluye fragmentos deregiones flanqueantes 5 y 3 del gen de EPO que no codifican para la proteína. El gen fue clonadoen un plásmido de expresion para célulaseucarioticas que tiene como unicos elementos de control de la expresion al promotor temprano de virus SV40 y su senal de poliadenilacion. Las célulasrecombinantes resultantes de la transfeccion con lasconst rucciones genéticas utilizadas produce inesperadamente más de 50 mg de EPO recombinante por litrode medio de cultivo y por día.The gene coding for human erythropoietin (EPO) was obtained from human genomic DNA. The gene used does not include flanking region fragments 5 and 3 of the EPO gene that do not code for the protein. The gene was cloned into an expression plasmid for seukaryotic cells that has as its only expression control elements the SV40 virus early promoter and its polyadenylation signal. The recombinant cells resulting from transfection with the genetic constructs used unexpectedly produce more than 50 mg of recombinant EPO per liter of culture medium per day.
Priority Applications (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP990100679 AR008363A1 (en) | 1999-02-23 | 1999-02-23 | RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCING CELL LINE AND RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCED BY THIS CELL. |
| BR9905867-7A BR9905867A (en) | 1998-11-06 | 1999-07-07 | Recombinant human erythropoietin-producing cell line and the recombinant human erythropoietin produced by this cell |
| CO99069968A CO5241323A1 (en) | 1998-11-06 | 1999-11-05 | RECOMBINANT HUMAN ERYTHROPOYETIN CELLULAR PRODUCER LINE. |
| AT99971863T ATE420199T1 (en) | 1998-11-06 | 1999-11-08 | HOST CELLS EXPRESSING RECOMBINANT HUMAN ERYTHROPOIETIN |
| US09/830,967 US6777205B1 (en) | 1998-11-06 | 1999-11-08 | Host cells expressing recombinant human erythropoietin |
| AU14704/00A AU1470400A (en) | 1998-11-06 | 1999-11-08 | Host cells expressing recombinant human erythropoietin |
| PCT/US1999/026238 WO2000028066A1 (en) | 1998-11-06 | 1999-11-08 | Host cells expressing recombinant human erythropoietin |
| ES99971863T ES2321048T3 (en) | 1998-11-06 | 1999-11-08 | HOSPED CELLS THAT EXPRESS RECOMBINANT HUMAN ERYTHROPOYETIN. |
| EP99971863A EP1124984B1 (en) | 1998-11-06 | 1999-11-08 | Host cells expressing recombinant human erythropoietin |
| JP2000581232A JP2002529100A (en) | 1998-11-06 | 1999-11-08 | Host cells expressing recombinant human erythropoietin |
| DE69940267T DE69940267D1 (en) | 1998-11-06 | 1999-11-08 | HOST CELLS EXPRESSING RECOMBINANT HUMANESE ERYTHROPOIETIN |
| CA2350335A CA2350335C (en) | 1998-11-06 | 1999-11-08 | Host cells expressing recombinant human erythropoietin |
| JP2010059896A JP2010213699A (en) | 1998-11-06 | 2010-03-16 | Host cell expressing recombinant human erythropoietin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP990100679 AR008363A1 (en) | 1999-02-23 | 1999-02-23 | RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCING CELL LINE AND RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCED BY THIS CELL. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR008363A1 true AR008363A1 (en) | 2000-01-19 |
Family
ID=45724368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990100679 AR008363A1 (en) | 1998-11-06 | 1999-02-23 | RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCING CELL LINE AND RECOMBINANT HUMAN ERYTHROPOYETINE PRODUCED BY THIS CELL. |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR008363A1 (en) |
-
1999
- 1999-02-23 AR ARP990100679 patent/AR008363A1/en not_active Application Discontinuation
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|---|---|---|---|
| FC | Refusal |