[go: up one dir, main page]

AP9901515A0 - Reagent system and kit or detecting HIV infected cells. - Google Patents

Reagent system and kit or detecting HIV infected cells.

Info

Publication number
AP9901515A0
AP9901515A0 APAP/P/1999/001515A AP9901515A AP9901515A0 AP 9901515 A0 AP9901515 A0 AP 9901515A0 AP 9901515 A AP9901515 A AP 9901515A AP 9901515 A0 AP9901515 A0 AP 9901515A0
Authority
AP
ARIPO
Prior art keywords
cells
present
infected cells
relates
kit
Prior art date
Application number
APAP/P/1999/001515A
Inventor
Robert A Hallowitz
Chester F King
Original Assignee
Bio Tech Imaging Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/732,782 external-priority patent/US5817458A/en
Priority claimed from US08/732,784 external-priority patent/US5714390A/en
Application filed by Bio Tech Imaging Inc filed Critical Bio Tech Imaging Inc
Publication of AP9901515A0 publication Critical patent/AP9901515A0/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

This invention related to blood collection and diagnostics. More particularly, the invention relates to blood collection and diagnostics utilizing techniques such as magnetic separation and photodetection. The present invention also relates to methods and an apparatus for detecting the presence of antigens displayed on the surface of cells. More preferably, the present invention relates to the detection of cells infected by human immunodeficiency virus (hiv)and related viruses. In accordance with the present invention, hiv-infected cells can be detected and separated from uninfected cells. In a preferred embodiment, separation is achieved by a magnetic field. By coating the infected cells with magnetic particles, transfer of the cells to a precise location is facilitated. A novel aspect os the present invention is a cartridge antigen test which allows for the collection and mixing of blood with reagents in one package, which can be viewed on a fluoresent microscope.
APAP/P/1999/001515A 1996-10-15 1997-10-15 Reagent system and kit or detecting HIV infected cells. AP9901515A0 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/732,782 US5817458A (en) 1996-10-15 1996-10-15 Reagent system for detecting HIV-infected peripheral blood lymphocytes in whole blood
US08/732,784 US5714390A (en) 1996-10-15 1996-10-15 Cartridge test system for the collection and testing of blood in a single step
PCT/US1997/018649 WO1998016101A1 (en) 1996-10-15 1997-10-15 Reagent system and kit for detecting hiv infected cells

Publications (1)

Publication Number Publication Date
AP9901515A0 true AP9901515A0 (en) 1999-06-30

Family

ID=27112456

Family Applications (1)

Application Number Title Priority Date Filing Date
APAP/P/1999/001515A AP9901515A0 (en) 1996-10-15 1997-10-15 Reagent system and kit or detecting HIV infected cells.

Country Status (5)

Country Link
EP (1) EP0933989A1 (en)
JP (1) JP2001503859A (en)
AP (1) AP9901515A0 (en)
AU (1) AU743937B2 (en)
WO (1) WO1998016101A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010039007A1 (en) * 1996-10-15 2001-11-08 Robert Hallowitz Methods and compositions for determining latent viral load
AU2001271643A1 (en) * 2000-06-30 2002-01-14 Bio-Tech Imaging, Inc. Methods for characterizing the viral infectivity status of host
FR2832507B1 (en) 2001-11-20 2004-02-20 Stago Diagnostica METHOD OF DETECTING ANALYTE (S) USING COLLOIDAL MAGNETIC PARTICLES
WO2004031209A2 (en) * 2002-10-01 2004-04-15 Functional Genetics, Inc. Anti-tsg101 antibodies and their uses for treatment of viral infections
WO2006009398A1 (en) * 2004-07-20 2006-01-26 Cgk Co., Ltd. System for detecting molecular interactions
CN107290534A (en) * 2017-07-12 2017-10-24 华讯方舟科技有限公司 A kind of blood cell capture chip and method
CN114908190A (en) * 2022-04-29 2022-08-16 深圳市血液中心(深圳市输血医学研究所) HIV DNA detection method

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4659678A (en) * 1982-09-29 1987-04-21 Serono Diagnostics Limited Immunoassay of antigens
FI844027A (en) * 1984-10-12 1986-04-13 Labsystems Oy IMMUNOLOGISKT BESTAEMNINGSFOERFARANDE.
EP0452342B1 (en) * 1988-12-28 1994-11-30 MILTENYI, Stefan Methods and materials for high gradient magnetic separation of biological materials
US5054499A (en) * 1989-03-27 1991-10-08 Swierczek Remi D Disposable skin perforator and blood testing device
US5541072A (en) * 1994-04-18 1996-07-30 Immunivest Corporation Method for magnetic separation featuring magnetic particles in a multi-phase system
US5231993A (en) * 1991-11-20 1993-08-03 Habley Medical Technology Corporation Blood sampler and component tester with guide member

Also Published As

Publication number Publication date
EP0933989A1 (en) 1999-08-11
JP2001503859A (en) 2001-03-21
AU4822497A (en) 1998-05-11
WO1998016101A1 (en) 1998-04-23
AU743937B2 (en) 2002-02-07

Similar Documents

Publication Publication Date Title
Constantine et al. HIV testing technologies after two decades of evolution
CA2272881C (en) Assay method for peptide specific t-cells
McHugh et al. Simultaneous detection of antibodies to cytomegalovirus and herpes simplex virus by using flow cytometry and a microsphere-based fluorescence immunoassay
US7129056B2 (en) Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood
AU655592B2 (en) Hepatitis C assay utilizing recombinant antigens
JPH11237381A (en) Method for fitting human or animal transplantation tissue to recipient and method for detecting tumor antigen in human or animal
AP9901515A0 (en) Reagent system and kit or detecting HIV infected cells.
Damhorst et al. Micro-and nanotechnology for HIV/AIDS diagnostics in resource-limited settings
Böni et al. Sensitive detection and quantification of particle‐associated reverse transcriptase in plasma of HIV‐1‐infected individuals by the product‐enhanced reverse transcriptase (PERT) assay
EP0902286A2 (en) Immunoassay utilizing two incubations with labelled antigen
CA2303123A1 (en) Method for detecting antibodies in a sample
WO1995023973A3 (en) Hiv immunoassay utilizing recombinant protein and synthetic peptide reagents
ES2094124T3 (en) IMMUNOLOGICAL TEST OF ANTIBODIES AGAINST HIV.
Nabatiyan et al. A lateral flow-based ultra-sensitive p24 HIV assay utilizing fluorescent microparticles
Mas et al. Reliability of a new recombinant immunoblot assay (RIBA HIV-1/HIV-2 SIA) as a supplemental (confirmatory) test for HIV-1 and HIV-2 infections
RU97115554A (en) DETECTION OF ANTIBODY PRODUCTION
Vallari et al. Rapid assay for simultaneous detection and differentiation of immunoglobulin G antibodies to human immunodeficiency virus type 1 (HIV-1) group M, HIV-1 group O, and HIV-2
Miller Profile of the MP Diagnostics HTLV Blot 2.4 test: a supplemental assay for the confirmation and differentiation of antibodies to HTLV-1 and HTLV-2
Asihene et al. Evaluation of rapid test for detection of antibody to human immunodeficiency virus type 1 and type 2
Sommerfelt et al. A simple semi-rapid HIV-1&2 confirmatory immunoassay using magnetic particles
CA2116026A1 (en) Hepatitis c assay utilizing recombinant antigens to c-100 region
RU2002100350A (en) Antibody analysis
Sirivichayakul et al. Evaluation of a 2-minute anti-human immunodeficiency virus (HIV) test using the autologous erythrocyte agglutination technique with populations differing in HIV prevalence
RU2234700C2 (en) Method for diagnosis of viral hepatitis b
Delaporte et al. Interpretation of antibodies reacting solely with human retroviral core proteins in western equatorial Africa