[go: up one dir, main page]

AP2003002886A0 - Constructs and methods for expression of recombinant HCV envelope proteins - Google Patents

Constructs and methods for expression of recombinant HCV envelope proteins

Info

Publication number
AP2003002886A0
AP2003002886A0 APAP/P/2003/002886A AP2003002886A AP2003002886A0 AP 2003002886 A0 AP2003002886 A0 AP 2003002886A0 AP 2003002886 A AP2003002886 A AP 2003002886A AP 2003002886 A0 AP2003002886 A0 AP 2003002886A0
Authority
AP
ARIPO
Prior art keywords
hcv envelope
methods
expression
envelope proteins
constructs
Prior art date
Application number
APAP/P/2003/002886A
Inventor
Erwin Sablon
Broekhoven Annie Van
Alfons Bosman
Erik Depla
Geert Deschamps
Original Assignee
Innogenetics Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innogenetics Nv filed Critical Innogenetics Nv
Priority claimed from PCT/BE2002/000062 external-priority patent/WO2002085932A2/en
Publication of AP2003002886A0 publication Critical patent/AP2003002886A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24223—Virus like particles [VLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Tires In General (AREA)

Abstract

The current invention relates to vectors and methods for efficient expression of HCV envelope proteins in eukaryotic cells. More particularly said vectors comprise the coding sequence for an avian lysozyme signal peptide or a functional equivalent thereof joined to a HCV envelope protein or a part thereof. Said avian lysozyme signal peptide is efficiently removed when the protein comprising said avian lysozyme signal peptide joined to a HCV envelope protein or a part thereof is expressed in a eukaryotic cell. Suitable eukaryotic cells include yeast cells such as Saccharomyces or Hansenula cells.
APAP/P/2003/002886A 2001-04-24 2002-04-24 Constructs and methods for expression of recombinant HCV envelope proteins AP2003002886A0 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01870088 2001-04-24
US30560401P 2001-07-17 2001-07-17
PCT/BE2002/000062 WO2002085932A2 (en) 2001-04-24 2002-04-24 Constructs and methods for expression of recombinant hcv envelope proteins

Publications (1)

Publication Number Publication Date
AP2003002886A0 true AP2003002886A0 (en) 2003-12-31

Family

ID=26077526

Family Applications (1)

Application Number Title Priority Date Filing Date
APAP/P/2003/002886A AP2003002886A0 (en) 2001-04-24 2002-04-24 Constructs and methods for expression of recombinant HCV envelope proteins

Country Status (5)

Country Link
AP (1) AP2003002886A0 (en)
BG (1) BG108373A (en)
CO (1) CO5650176A2 (en)
HR (1) HRP20030946A2 (en)
IL (1) IL158422A0 (en)

Also Published As

Publication number Publication date
IL158422A0 (en) 2004-05-12
CO5650176A2 (en) 2006-06-30
BG108373A (en) 2004-12-30
HRP20030946A2 (en) 2005-10-31

Similar Documents

Publication Publication Date Title
MXPA03009626A (en) Constructs and methods for expression of recombinant hcv envelope proteins.
WO2003100021A3 (en) Methods and dna constructs for high yield production of polypeptides
EP0542863A4 (en) Expression of recombinant polypeptides with improved purification
EP1304382A3 (en) Production of peptides in plants as viral coat protein fusions
ATE546533T1 (en) RECOMBINANT EXPRESSION OF PROTEINS IN A DISULFIDE-BRIDGED, TWO-CHAIN FORM
IN190327B (en)
DK0531404T3 (en) Ubiquitin-specific protease
PL372849A1 (en) Use of transthyretin peptide/protein fusions to increase the serum half-life of pharmacologically active peptides/proteins
CY1110670T1 (en) IMPROVEMENT OF THE homogeneity and secretion of recombinant proteins in mammalian systems
AU7873798A (en) N-terminally extended proteins expressed in yeast
AP2003002886A0 (en) Constructs and methods for expression of recombinant HCV envelope proteins
AR031336A1 (en) METHODS FOR THE LARGE SCALE OF PROTEINS IN PROCARIOTS
AU5776799A (en) Modified hcv peptide vaccines
WO2003100022A3 (en) Methods and dna constructs for high yield production of polypeptides
ATE295890T1 (en) VECTOR FOR EXPRESSING N-TERMINALLY EXTENDED PROTEINS IN YEAST CELLS
WO1999002708A8 (en) Fusion proteins comprising sequences derived from bovine if1 atpase inhibitor protein
DE69333304D1 (en) INCREASED PRODUCTION OF SECRETED PROTEINS BY RECOMBINANT EUKARYOTIC CELLS
WO2003014363A8 (en) Vector for site-specific integration of heterologous dna sequences into the rdn a sites of methylotrophic yeasts
WO2002048187A3 (en) Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins
ATE354663T1 (en) A MYCOBACTERIUM TUBERCULOSIS CODING SEQUENCE FOR EXPRESSING HETEROLOGUE PROTEINS
KR970059277A (en) Production method of human interleukin-11 using yeast
DE60207157D1 (en) Purification method based on the binding of enzymes and labeled peptides
WO2000002909A3 (en) Amphiphilic peptides derived from the cytoplasmic tail of viral envelope proteins
CO5070672A1 (en) HUMAN PROTEIN C POLYPEPTIDE
WO2006013367A3 (en) Chimeric heamaglutinin proteins and uses thereof