To analyze crestal bone loss changes that may affect the gingival height around an implant and bo... more To analyze crestal bone loss changes that may affect the gingival height around an implant and bone loss in interproximal areas. When implants are placed adjacent to one another, interimplant bone loss around 1 implant may combine with the implant bone loss around the adjacent implant to affect the crestal bone loss interproximally. The primary objective of this study was to evaluate histologically and histomorphometrically the effect that this implant design with a horizontally displaced implant-abutment junction has on the height of the crest of bone, between adjacent implants separated by 2 different distances: 4 mm and 3 mm. A secondary objective was to evaluate the percentage of bone-to-implant contact, interproximal soft tissue height, location of the junctional epithelium, and length of connective tissue contact to abutment and/or implant, for the 2 different distances. Results showed that the interproximal bone loss measured from the edge of the implant platform to the bone ...
Dental materials : official publication of the Academy of Dental Materials, Jan 13, 2015
Enamel matrix derivative (EMD) is an effective biomaterial for periodontal tissue regeneration an... more Enamel matrix derivative (EMD) is an effective biomaterial for periodontal tissue regeneration and might stimulate angiogenesis. In order to clarify mechanisms underlying its biological activity, we separated two EMD fractions with different molecular weight protein components and investigated their effects on human umbilical vein endothelial cells (HUVECs) in vitro. Fraction Low-Molecular Weight (LMW) included proteins with a molecular weight (M.W.)<8kDa. Fraction LMW-depleted included proteins with M.W.>8kDa and lower than approximately 55kDa. The effect of EMD fractions on proliferation/viability, apoptosis, migration and expression of angiopoetin-2 (ang-2), von Willebrand factor (vWF), E-selectin, intracellular adhesion molecules 1 (ICAM-1), vascular endothelial growth factor (VEGF) receptors Flt-1 and KDR was investigated. The proliferation/viability of HUVECs was inhibited by both LMW and LMW-depleted at concentrations 100μg/ml, whereas EMD slightly increased cell prolif...
To analyze crestal bone loss changes that may affect the gingival height around an implant and bo... more To analyze crestal bone loss changes that may affect the gingival height around an implant and bone loss in interproximal areas. When implants are placed adjacent to one another, interimplant bone loss around 1 implant may combine with the implant bone loss around the adjacent implant to affect the crestal bone loss interproximally. The primary objective of this study was to evaluate histologically and histomorphometrically the effect that this implant design with a horizontally displaced implant-abutment junction has on the height of the crest of bone, between adjacent implants separated by 2 different distances: 4 mm and 3 mm. A secondary objective was to evaluate the percentage of bone-to-implant contact, interproximal soft tissue height, location of the junctional epithelium, and length of connective tissue contact to abutment and/or implant, for the 2 different distances. Results showed that the interproximal bone loss measured from the edge of the implant platform to the bone ...
Recently, a chemically modified ultra-hydrophilic sand-blasted, large grit and acid-etched (modSL... more Recently, a chemically modified ultra-hydrophilic sand-blasted, large grit and acid-etched (modSLA) titanium surface has been introduced in order to enhance bone apposition. Indeed, preliminary preclinical and clinical data have indicated that modSLA implants may enhance bone apposition during early stages of wound healing. These positive effects on bone regeneration might be probably mainly due to the hydrophilic surface properties noted for modSLA which ensured a stabilization of the blood clot. The aim of the present review article is to evaluate, based on the currently available evidence, the potential impact of modSLA surfaces for implant dentistry.
The aim of this study was to investigate bone regeneration following application of a novel bipha... more The aim of this study was to investigate bone regeneration following application of a novel biphasic calcium phosphate (BCP I) composed of microstructured granules of 90% β-tricalcium phosphate (β-TCP)/10% hydroxyapatite (HA) compared to BCP non-microstructured biphasic calcium phosphate with a composite of 60% hydroxyapatite/40% β-TCP (BCP II) and a deproteinized bovine bone mineral (DBBM) at surgically created defects in the mandible of minipigs in a combined approach with guided bone regeneration (GBR). Sixteen minipigs were used for the study. Lower premolars P2, P3, P4 and first molar M1 were extracted. Following 3 months of healing, two defects with a width and depth of 7 mm were created bilaterally in the mandible. The different grafting materials were randomly placed in the created defects and covered by means of a collagen membrane. After 3 and 8 weeks, biopsies were sampled. All specimens were evaluated with descriptive histology and histomorphometric evaluations complemented by micro-CT scan analysis. All three biomaterials presented with higher bone volume at 8 weeks compared to 3 weeks (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0442). BCP I and DBBM demonstrated a significant higher amount of bone formation compared to BCP II at 8 weeks (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0328). BCP I also demonstrated a significant higher percentage of remaining graft volume compared to the other test groups both at 3 and 8 weeks (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001 to P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0003). Congruently, defects containing BCP I showed a significant higher amount of mineralized tissue compared to the other groups. All the three test materials performed well with regard to bone formation at 8 weeks. BCP I showed significant higher amounts of newly formed bone despite a higher remaining graft volume compared to the other groups. With regard to the regenerative outcome, all the three materials can be recommended for clinical use.
Chronic kidney disease (CKD) has become a worldwide public health problem. However, its effect on... more Chronic kidney disease (CKD) has become a worldwide public health problem. However, its effect on osseointegration of dental implants is largely unknown. The aim of this study is to investigate whether CKD impairs the quality of the osseointegration of titanium implants. Uremia was induced by 5/6 nephrectomy in mice, and serum levels of BUN, FGF23, PTH and ALP were significantly increased. For in vitro tests, bone marrow mesenchymal stem cells (BMMSCs) were obtained and cultured on titanium discs. There was no significant difference in term of expression of osteogenic marker genes including Osx, Col-1, Ocn, and Opn, as quantified by qPCR. Moreover, Alizarin Red S staining showed comparable mineralized nodules formation. Histomorphometrical analysis of experimental implants inserted in the femurs of CKD mice revealed a trend of decreased BIC ratio at 2-week healing. The strength of bone-implant integration, as measured by a push-in method, was significantly lower for the CKD group at 2 weeks, although a comparable level was reached at 4 weeks. These results demonstrated that CKD only negatively affects the osseointegration of titanium implants at the early stage.
Abstract-The technique of immediate implant placement after extraction has been conceived for pre... more Abstract-The technique of immediate implant placement after extraction has been conceived for preserving the residual bone support and soft tissue morphology. Nowadays this procedure is still not predictable and presents inconveniences both for the patient and the dentist. Therefore, the healing process around a dental implant placed into an extraction socket needs to be deeply investigated in order to increase the predictability of this surgical approach. The aim of the present investigation was to evaluate the healing of bone defects (fresh extraction sockets) following implant installation with flap elevation and primary closure compared to implant installation without flap elevation. This study evaluated by histologic and histomorphometric analyses tissue healing around dental implants with marginal bone defects with and without flap elevation 1 week, 4 weeks and 12 weeks following implantation in the dogs. The main qualitative findings showed that after 1 week of implantation, ...
To analyze crestal bone loss changes that may affect the gingival height around an implant and bo... more To analyze crestal bone loss changes that may affect the gingival height around an implant and bone loss in interproximal areas. When implants are placed adjacent to one another, interimplant bone loss around 1 implant may combine with the implant bone loss around the adjacent implant to affect the crestal bone loss interproximally. The primary objective of this study was to evaluate histologically and histomorphometrically the effect that this implant design with a horizontally displaced implant-abutment junction has on the height of the crest of bone, between adjacent implants separated by 2 different distances: 4 mm and 3 mm. A secondary objective was to evaluate the percentage of bone-to-implant contact, interproximal soft tissue height, location of the junctional epithelium, and length of connective tissue contact to abutment and/or implant, for the 2 different distances. Results showed that the interproximal bone loss measured from the edge of the implant platform to the bone ...
Dental materials : official publication of the Academy of Dental Materials, Jan 13, 2015
Enamel matrix derivative (EMD) is an effective biomaterial for periodontal tissue regeneration an... more Enamel matrix derivative (EMD) is an effective biomaterial for periodontal tissue regeneration and might stimulate angiogenesis. In order to clarify mechanisms underlying its biological activity, we separated two EMD fractions with different molecular weight protein components and investigated their effects on human umbilical vein endothelial cells (HUVECs) in vitro. Fraction Low-Molecular Weight (LMW) included proteins with a molecular weight (M.W.)<8kDa. Fraction LMW-depleted included proteins with M.W.>8kDa and lower than approximately 55kDa. The effect of EMD fractions on proliferation/viability, apoptosis, migration and expression of angiopoetin-2 (ang-2), von Willebrand factor (vWF), E-selectin, intracellular adhesion molecules 1 (ICAM-1), vascular endothelial growth factor (VEGF) receptors Flt-1 and KDR was investigated. The proliferation/viability of HUVECs was inhibited by both LMW and LMW-depleted at concentrations 100μg/ml, whereas EMD slightly increased cell prolif...
To analyze crestal bone loss changes that may affect the gingival height around an implant and bo... more To analyze crestal bone loss changes that may affect the gingival height around an implant and bone loss in interproximal areas. When implants are placed adjacent to one another, interimplant bone loss around 1 implant may combine with the implant bone loss around the adjacent implant to affect the crestal bone loss interproximally. The primary objective of this study was to evaluate histologically and histomorphometrically the effect that this implant design with a horizontally displaced implant-abutment junction has on the height of the crest of bone, between adjacent implants separated by 2 different distances: 4 mm and 3 mm. A secondary objective was to evaluate the percentage of bone-to-implant contact, interproximal soft tissue height, location of the junctional epithelium, and length of connective tissue contact to abutment and/or implant, for the 2 different distances. Results showed that the interproximal bone loss measured from the edge of the implant platform to the bone ...
Recently, a chemically modified ultra-hydrophilic sand-blasted, large grit and acid-etched (modSL... more Recently, a chemically modified ultra-hydrophilic sand-blasted, large grit and acid-etched (modSLA) titanium surface has been introduced in order to enhance bone apposition. Indeed, preliminary preclinical and clinical data have indicated that modSLA implants may enhance bone apposition during early stages of wound healing. These positive effects on bone regeneration might be probably mainly due to the hydrophilic surface properties noted for modSLA which ensured a stabilization of the blood clot. The aim of the present review article is to evaluate, based on the currently available evidence, the potential impact of modSLA surfaces for implant dentistry.
The aim of this study was to investigate bone regeneration following application of a novel bipha... more The aim of this study was to investigate bone regeneration following application of a novel biphasic calcium phosphate (BCP I) composed of microstructured granules of 90% β-tricalcium phosphate (β-TCP)/10% hydroxyapatite (HA) compared to BCP non-microstructured biphasic calcium phosphate with a composite of 60% hydroxyapatite/40% β-TCP (BCP II) and a deproteinized bovine bone mineral (DBBM) at surgically created defects in the mandible of minipigs in a combined approach with guided bone regeneration (GBR). Sixteen minipigs were used for the study. Lower premolars P2, P3, P4 and first molar M1 were extracted. Following 3 months of healing, two defects with a width and depth of 7 mm were created bilaterally in the mandible. The different grafting materials were randomly placed in the created defects and covered by means of a collagen membrane. After 3 and 8 weeks, biopsies were sampled. All specimens were evaluated with descriptive histology and histomorphometric evaluations complemented by micro-CT scan analysis. All three biomaterials presented with higher bone volume at 8 weeks compared to 3 weeks (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0442). BCP I and DBBM demonstrated a significant higher amount of bone formation compared to BCP II at 8 weeks (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0328). BCP I also demonstrated a significant higher percentage of remaining graft volume compared to the other test groups both at 3 and 8 weeks (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001 to P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0003). Congruently, defects containing BCP I showed a significant higher amount of mineralized tissue compared to the other groups. All the three test materials performed well with regard to bone formation at 8 weeks. BCP I showed significant higher amounts of newly formed bone despite a higher remaining graft volume compared to the other groups. With regard to the regenerative outcome, all the three materials can be recommended for clinical use.
Chronic kidney disease (CKD) has become a worldwide public health problem. However, its effect on... more Chronic kidney disease (CKD) has become a worldwide public health problem. However, its effect on osseointegration of dental implants is largely unknown. The aim of this study is to investigate whether CKD impairs the quality of the osseointegration of titanium implants. Uremia was induced by 5/6 nephrectomy in mice, and serum levels of BUN, FGF23, PTH and ALP were significantly increased. For in vitro tests, bone marrow mesenchymal stem cells (BMMSCs) were obtained and cultured on titanium discs. There was no significant difference in term of expression of osteogenic marker genes including Osx, Col-1, Ocn, and Opn, as quantified by qPCR. Moreover, Alizarin Red S staining showed comparable mineralized nodules formation. Histomorphometrical analysis of experimental implants inserted in the femurs of CKD mice revealed a trend of decreased BIC ratio at 2-week healing. The strength of bone-implant integration, as measured by a push-in method, was significantly lower for the CKD group at 2 weeks, although a comparable level was reached at 4 weeks. These results demonstrated that CKD only negatively affects the osseointegration of titanium implants at the early stage.
Abstract-The technique of immediate implant placement after extraction has been conceived for pre... more Abstract-The technique of immediate implant placement after extraction has been conceived for preserving the residual bone support and soft tissue morphology. Nowadays this procedure is still not predictable and presents inconveniences both for the patient and the dentist. Therefore, the healing process around a dental implant placed into an extraction socket needs to be deeply investigated in order to increase the predictability of this surgical approach. The aim of the present investigation was to evaluate the healing of bone defects (fresh extraction sockets) following implant installation with flap elevation and primary closure compared to implant installation without flap elevation. This study evaluated by histologic and histomorphometric analyses tissue healing around dental implants with marginal bone defects with and without flap elevation 1 week, 4 weeks and 12 weeks following implantation in the dogs. The main qualitative findings showed that after 1 week of implantation, ...
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