The present study aims to understand the existing genetic diversity and structure of six native c... more The present study aims to understand the existing genetic diversity and structure of six native cattle breeds (Rathi, Tharparkar, Nagori, Mewati, Gir, and Kankrej) adapted to the north-western arid and semi-arid region of India based on microsatellite loci. Various diversity estimates, mean number of alleles (12.84); effective number of alleles (5.02); gene diversity (0.769), and observed heterozygosity (0.667) reflected the existence of substantial within-breed diversity in all the investigated cattle breeds. Mean estimates of F-statistics: F(IT) = 0.144 ± 0.023, F(IS) = 0.071 ± 0.021, and F(ST) = 0.078 ± 0.014 were significantly different from zero (P < 0.05). The interbreed relationships indicated moderate level of breed differentiation between the six cattle breeds with least differentiation between Kankrej-Mewati pair. The phylogeny structuring further supported close grouping of Kankrej and Mewati breeds. Correspondence analysis plotted Rathi, Tharparkar, and Gir individuals into three separate areas of multivariate space; whereas, Kankrej, Mewati, and Nagori cattle showed low breed specific clustering. This reflected the existence of discrete genetic structure for Tharparkar, Rathi, and Gir, the prominent dairy breeds of the region; whereas, admixture was observed for Kankrej, Mewati, and Nagori individuals.
In the present study, efforts were made to sequence characterize the selected exonic region (18â€... more In the present study, efforts were made to sequence characterize the selected exonic region (18–21) of ATP1A1 gene to identify variations/SNPs in different breeds of Indian riverine buffaloes and native cattle. The sequence characterization of selected intronic/exonic region (17–21) of ATP1A1 gene was carried out in a total of 120 samples which included 6 animals each of 8 buffalo breeds and 72 animals of 12 cattle breeds. Genomic DNA was extracted from the whole blood by enzymatic digestion using proteinase K using phenol:chloroform method.. Three sets of primers were designed using Primer3 software to amplify genomic region from intron 17 to intron 21 of ATP1A1 gene in both cattle and buffaloes. The amplified products were purified by enzymatic method and purified PCR products were sequenced using forward primers in an ABI 3100 Automated DNA Sequencer. The chromatogram of each sequence obtained was checked manually. Base calling was performed with Phred and contig assembly was...
This study aims to assess the genetic diversity and population structure of two major zebu dairy ... more This study aims to assess the genetic diversity and population structure of two major zebu dairy breeds (Tharparkar and Rathi) adapted to the arid region of Rajasthan state of India. Various variability estimates indicate the existence of sufficient within-breed genetic diversity. Mean estimates of F-statistics are significantly different from zero: F (IS) = 0.112 +/- 0.029, F (IT) = 0.169 +/- 0.033, F (ST) = 0.065 +/- 0.017. The overall positive value of F (IS) (0.112) and an F (IT) value (0.169) that is more than the F (ST) (0.065) indicate departure from random mating. The drift-based estimates reflect a moderate yet significant level of breed differentiation between the Tharparkar and Rathi breeds. The evaluation of an exact test, showing that allele frequencies across all the loci differed significantly, supports the population differentiation. This is paralleled by the outcome of neighbor-joining clustering based on allele-sharing distance measures. The allocation of a high percentage of individuals (95.7%) to their population of origin and correspondence analysis further substantiates the existence of a cohesive genetic structure in both the breeds.
Proteases play a significant role in milk and its products by affecting flavor, texture and longe... more Proteases play a significant role in milk and its products by affecting flavor, texture and longevity. The expression of endogenous proteases varies across different stages of lactation. The study was conducted to understand the transcriptional pattern of different classes of protease-pathways associated genes (CTSB, CTSD, CTSH, CTSL, CTSK, CTSS, CTSZ, PLAU, PLAT) and potential protease inhibitors (SERPIN E2 and SERPIN F2) in 40 milk somatic cells (MSC) samples isolated during early, peak, mid and late lactation stages of Sahiwal cows and Murrah buffaloes - the two most important dairy breeds of India. In Sahiwal cows, except CTSK and PLAU, the expression of other proteases class was not affected significantly (p > 0.05) across lactation stages. However, in Murrah buffaloes, the expression of different proteases increased as the lactation progressed. Most of the proteases showed lower expression during early and peak lactation stages while their expression tends to increase during mid to late lactation stages. The overall trend was somewhat similar in both the dairy species albeit the level of expression was higher in buffalo MSC as compared to cow MSC. The study has provided valuable information on expression kinetics of different proteases in milk somatic cells of two major dairy breeds of India.
An attempt was made to assess the phenotypic variability and study the management practices of in... more An attempt was made to assess the phenotypic variability and study the management practices of indigenous swamp buffalo 'Bhangor' population from the north-eastern state of Tripura. Bhangor buffalo animals have morphological features and behaviour similar to typical swamp buffalo. A survey was conducted in villages covering entire state, to record the phenotypic information. Adult animals are medium sized with compact body and used for draught and meat purpose. The horn is like sickle shaped (curved) with flat broad base and mostly corrugated, and curved upward to form a circle. Recently, for the first time, we had characterised Bhangor buffalo based on cytogenetic analysis as swamp type showing 2N=48 chromosomes. In present study, we had evaluated the reproductive performance and management practices. The buffalo provide valuable draught power in paddy fields. The indigenous population are important and much needed for the local communities as they are most suitably adapted...
Quantitative real-time PCR has emerged as a highly sensitive and widely used method for detection... more Quantitative real-time PCR has emerged as a highly sensitive and widely used method for detection of gene expression profiles, via which accurate detection depends on reliable normalization. Since no single control is appropriate for all experimental treatments, it is generally advocated to select suitable internal controls prior to use for normalization. This study reported the evaluation of the expression stability of twelve potential reference genes in different tissue/organs and six fruit developmental stages of Litsea cubeba in order to screen the superior internal reference genes for data normalization. Two softwares-geNorm, and NormFinder-were used to identify stability of these candidate genes. The cycle threshold difference and coefficient of variance were also calculated to evaluate the expression stability of candidate genes. F-BOX, EF1α, UBC, and TUA were selected as the most stable reference genes across 11 sample pools. F-BOX, EF1α, and EIF4α exhibited the highest expression stability in different tissue/organs and different fruit developmental stages. Besides, a combination of two stable reference genes would be sufficient for gene expression normalization in different fruit developmental stages. In addition, the relative expression profiles of DXS and DXR were evaluated by EF1α, UBC, and SAMDC. The results further validated the reliability of stable reference genes and also highlighted the importance of selecting suitable internal controls for L. cubeba. These reference genes will be of great importance for transcript normalization in future gene expression studies on L. cubeba.
In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk hav... more In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk have drawn a lot of attention world over because of their proposed impact on human health. In order to evaluate the transcriptional modulation of target genes through RT-qPCR in response to these peptides, availability of appropriate reference or internal control genes (ICGs) will be the key. The present study was planned to identify a panel of stable ICGs in the liver tissue of C57BL/6 mice injected with BCM7/BCM9 cow milk peptides for 3 weeks. A total of ten candidate genes were evaluated as potential ICGs by assessing their expression stability using software suites; geNorm, NormFinder and BestKeeper. The suitability of the identified ICGs was validated by assessing the relative expression levels of target genes, HP and Cu/Zn SOD. Based on geNorm, PPIA and SDHA gene pair was identified to be most stably expressed in liver tissue during the animal trials. Similarly, NormFinder analysis al...
Around parturition, animal undergoes a state of immune-suppression and become more susceptible to... more Around parturition, animal undergoes a state of immune-suppression and become more susceptible to pathogens. This study was aimed to identify appropriate internal control genes (ICG) for transcriptional studies in polymorphoneutrophils (PMN)of Sahiwal (Bos indicus) cows during the periparturient period. The study involved 4 periparturient Sahiwal cows (–21 days to 0 day to +21 days relative to parturition) and 10 known internal control genes (ICG)from different functional classes. ForqPCR reaction following conditions were employed: 2 min at 50°C, 10 min at 95 °C, 40 cycles of 15 s at 95 °C (denaturation) and 1 min at 60°C (annealing + extension).To measure the transcript stability of 10 ICG genes, threesoftwareprogrammes;geNorm, Normfinder and BestKeeper were used. All the genes showed acceptable expression stability as per the recommended threshold values. The geNorm analysis showed RPS9, RPS15A and RPS23 genes to be most stably expressed while HMBS and HPRT1 showed least stabilit...
The present study aims to understand the existing genetic diversity and structure of six native c... more The present study aims to understand the existing genetic diversity and structure of six native cattle breeds (Rathi, Tharparkar, Nagori, Mewati, Gir, and Kankrej) adapted to the north-western arid and semi-arid region of India based on microsatellite loci. Various diversity estimates, mean number of alleles (12.84); effective number of alleles (5.02); gene diversity (0.769), and observed heterozygosity (0.667) reflected the existence of substantial within-breed diversity in all the investigated cattle breeds. Mean estimates of F-statistics: F(IT) = 0.144 ± 0.023, F(IS) = 0.071 ± 0.021, and F(ST) = 0.078 ± 0.014 were significantly different from zero (P < 0.05). The interbreed relationships indicated moderate level of breed differentiation between the six cattle breeds with least differentiation between Kankrej-Mewati pair. The phylogeny structuring further supported close grouping of Kankrej and Mewati breeds. Correspondence analysis plotted Rathi, Tharparkar, and Gir individuals into three separate areas of multivariate space; whereas, Kankrej, Mewati, and Nagori cattle showed low breed specific clustering. This reflected the existence of discrete genetic structure for Tharparkar, Rathi, and Gir, the prominent dairy breeds of the region; whereas, admixture was observed for Kankrej, Mewati, and Nagori individuals.
In the present study, efforts were made to sequence characterize the selected exonic region (18â€... more In the present study, efforts were made to sequence characterize the selected exonic region (18–21) of ATP1A1 gene to identify variations/SNPs in different breeds of Indian riverine buffaloes and native cattle. The sequence characterization of selected intronic/exonic region (17–21) of ATP1A1 gene was carried out in a total of 120 samples which included 6 animals each of 8 buffalo breeds and 72 animals of 12 cattle breeds. Genomic DNA was extracted from the whole blood by enzymatic digestion using proteinase K using phenol:chloroform method.. Three sets of primers were designed using Primer3 software to amplify genomic region from intron 17 to intron 21 of ATP1A1 gene in both cattle and buffaloes. The amplified products were purified by enzymatic method and purified PCR products were sequenced using forward primers in an ABI 3100 Automated DNA Sequencer. The chromatogram of each sequence obtained was checked manually. Base calling was performed with Phred and contig assembly was...
This study aims to assess the genetic diversity and population structure of two major zebu dairy ... more This study aims to assess the genetic diversity and population structure of two major zebu dairy breeds (Tharparkar and Rathi) adapted to the arid region of Rajasthan state of India. Various variability estimates indicate the existence of sufficient within-breed genetic diversity. Mean estimates of F-statistics are significantly different from zero: F (IS) = 0.112 +/- 0.029, F (IT) = 0.169 +/- 0.033, F (ST) = 0.065 +/- 0.017. The overall positive value of F (IS) (0.112) and an F (IT) value (0.169) that is more than the F (ST) (0.065) indicate departure from random mating. The drift-based estimates reflect a moderate yet significant level of breed differentiation between the Tharparkar and Rathi breeds. The evaluation of an exact test, showing that allele frequencies across all the loci differed significantly, supports the population differentiation. This is paralleled by the outcome of neighbor-joining clustering based on allele-sharing distance measures. The allocation of a high percentage of individuals (95.7%) to their population of origin and correspondence analysis further substantiates the existence of a cohesive genetic structure in both the breeds.
Proteases play a significant role in milk and its products by affecting flavor, texture and longe... more Proteases play a significant role in milk and its products by affecting flavor, texture and longevity. The expression of endogenous proteases varies across different stages of lactation. The study was conducted to understand the transcriptional pattern of different classes of protease-pathways associated genes (CTSB, CTSD, CTSH, CTSL, CTSK, CTSS, CTSZ, PLAU, PLAT) and potential protease inhibitors (SERPIN E2 and SERPIN F2) in 40 milk somatic cells (MSC) samples isolated during early, peak, mid and late lactation stages of Sahiwal cows and Murrah buffaloes - the two most important dairy breeds of India. In Sahiwal cows, except CTSK and PLAU, the expression of other proteases class was not affected significantly (p > 0.05) across lactation stages. However, in Murrah buffaloes, the expression of different proteases increased as the lactation progressed. Most of the proteases showed lower expression during early and peak lactation stages while their expression tends to increase during mid to late lactation stages. The overall trend was somewhat similar in both the dairy species albeit the level of expression was higher in buffalo MSC as compared to cow MSC. The study has provided valuable information on expression kinetics of different proteases in milk somatic cells of two major dairy breeds of India.
An attempt was made to assess the phenotypic variability and study the management practices of in... more An attempt was made to assess the phenotypic variability and study the management practices of indigenous swamp buffalo 'Bhangor' population from the north-eastern state of Tripura. Bhangor buffalo animals have morphological features and behaviour similar to typical swamp buffalo. A survey was conducted in villages covering entire state, to record the phenotypic information. Adult animals are medium sized with compact body and used for draught and meat purpose. The horn is like sickle shaped (curved) with flat broad base and mostly corrugated, and curved upward to form a circle. Recently, for the first time, we had characterised Bhangor buffalo based on cytogenetic analysis as swamp type showing 2N=48 chromosomes. In present study, we had evaluated the reproductive performance and management practices. The buffalo provide valuable draught power in paddy fields. The indigenous population are important and much needed for the local communities as they are most suitably adapted...
Quantitative real-time PCR has emerged as a highly sensitive and widely used method for detection... more Quantitative real-time PCR has emerged as a highly sensitive and widely used method for detection of gene expression profiles, via which accurate detection depends on reliable normalization. Since no single control is appropriate for all experimental treatments, it is generally advocated to select suitable internal controls prior to use for normalization. This study reported the evaluation of the expression stability of twelve potential reference genes in different tissue/organs and six fruit developmental stages of Litsea cubeba in order to screen the superior internal reference genes for data normalization. Two softwares-geNorm, and NormFinder-were used to identify stability of these candidate genes. The cycle threshold difference and coefficient of variance were also calculated to evaluate the expression stability of candidate genes. F-BOX, EF1α, UBC, and TUA were selected as the most stable reference genes across 11 sample pools. F-BOX, EF1α, and EIF4α exhibited the highest expression stability in different tissue/organs and different fruit developmental stages. Besides, a combination of two stable reference genes would be sufficient for gene expression normalization in different fruit developmental stages. In addition, the relative expression profiles of DXS and DXR were evaluated by EF1α, UBC, and SAMDC. The results further validated the reliability of stable reference genes and also highlighted the importance of selecting suitable internal controls for L. cubeba. These reference genes will be of great importance for transcript normalization in future gene expression studies on L. cubeba.
In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk hav... more In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk have drawn a lot of attention world over because of their proposed impact on human health. In order to evaluate the transcriptional modulation of target genes through RT-qPCR in response to these peptides, availability of appropriate reference or internal control genes (ICGs) will be the key. The present study was planned to identify a panel of stable ICGs in the liver tissue of C57BL/6 mice injected with BCM7/BCM9 cow milk peptides for 3 weeks. A total of ten candidate genes were evaluated as potential ICGs by assessing their expression stability using software suites; geNorm, NormFinder and BestKeeper. The suitability of the identified ICGs was validated by assessing the relative expression levels of target genes, HP and Cu/Zn SOD. Based on geNorm, PPIA and SDHA gene pair was identified to be most stably expressed in liver tissue during the animal trials. Similarly, NormFinder analysis al...
Around parturition, animal undergoes a state of immune-suppression and become more susceptible to... more Around parturition, animal undergoes a state of immune-suppression and become more susceptible to pathogens. This study was aimed to identify appropriate internal control genes (ICG) for transcriptional studies in polymorphoneutrophils (PMN)of Sahiwal (Bos indicus) cows during the periparturient period. The study involved 4 periparturient Sahiwal cows (–21 days to 0 day to +21 days relative to parturition) and 10 known internal control genes (ICG)from different functional classes. ForqPCR reaction following conditions were employed: 2 min at 50°C, 10 min at 95 °C, 40 cycles of 15 s at 95 °C (denaturation) and 1 min at 60°C (annealing + extension).To measure the transcript stability of 10 ICG genes, threesoftwareprogrammes;geNorm, Normfinder and BestKeeper were used. All the genes showed acceptable expression stability as per the recommended threshold values. The geNorm analysis showed RPS9, RPS15A and RPS23 genes to be most stably expressed while HMBS and HPRT1 showed least stabilit...
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