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Multiplexed reverse-transcriptase quantitative polymerase chain reaction using plasmonic nanoparticles for point-of-care COVID-19 diagnosis
Quantitative polymerase chain reaction (qPCR) offers the capabilities of real-time monitoring of amplified products, fast detection, and quantitation...
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Development of reverse transcriptase-recombinase polymerase amplification (RT-RPA) assay for rapid detection of large cardamom chirke virus
Large cardamom chirke virus (LCCV) causing chirke disease of large cardamom is a major production constraint of this crop. Rapid and accurate...
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Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction
Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), a major development in PCR technology, is a powerful and sensitive...
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Development of an isothermal reverse transcriptase-recombinase polymerase amplification assay for the rapid diagnosis of four viroids in different grape cultivars
Rapid, isothermal detection is possible using recombinase polymerase amplification assay (RPA) offering great specificity plus sensitivity. An RPA...
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Polymerase Chain Reaction: An Indispensable Molecular Biology Tool of 21st Century
Due to its universal applications, Polymerase Chain Reaction (PCR) became an essential technique in various scientific branches such as microbiology,...
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Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses
The reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is considered to be the gold standard for gene expression...
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Ultrasensitive Reverse Transcriptase Loop-Mediated Isothermal Amplification (US-LAMP)-Based Detection of Malaria Infection from Dried Blood Spots
Submicroscopic malaria diagnosis requires highly sensitive tools instead of the conventional microscopy and rapid diagnostic tests (RDTs). While...
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Novel Isothermal Reverse Transcription Polymerase Spiral Reaction (RT-PSR) Assay for the Detection of Newcastle Disease Virus in Avian Species
Newcastle disease in avian species is caused by Newcastle disease virus (NDV). It is responsible for large economic losses to the poultry industry....
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Polymerase Chain Reaction: Principle, Technique and Applications in Pathology
Polymerase chain reaction (PCR) is one of the most important techniques in molecular pathology by which the single or the pieces of target DNA are...
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Expression of thermostable MMLV reverse transcriptase in Escherichia coli by directed mutation
The functionality of Moloney murine leukemia virus reverse transcriptase (MMLV RT) will increase with the improvement of its solubility and thermal...
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Association between human telomerase reverse transcriptase (hTERT) MNS16A polymorphism and risk of breast cancer
BackgroundIt is well known that telomerase activity is suppressed in normal human tissues and reactivated in tumors, suggesting that the human...
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Comparison of clinician diagnosis of COVID-19 with real time polymerase chain reaction in an adult-representative population in Sweden
BackgroundDue to the high transmissibility of SARS-CoV-2, accurate diagnosis is essential for effective infection control, but the gold standard,...
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Detection of Foodborne RNA Viruses by Reverse Transcriptase Droplet Digital PCR
Foodborne viruses remain the largest cause of human gastroenteritis and one of the largest contributors to foodborne illnesses worldwide. Currently,...
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Structural Information and Drug–Enzyme Interaction of the Non-Nucleoside Reverse Transcriptase Inhibitors Based on Computational Chemistry Approaches
Non-nucleoside reverse transcriptase inhibitors, such as nevirapine, TIBO, HEPT, and efavirenz, are very specific to HIV-1 reverse transcriptase...
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Generation of an Avian Myeloblastosis Virus (AMV) Reverse Transcriptase Prime Editor
Prime editing (PE) is a novel, double-strand break (DSB)-independent gene editing technology that represents an exciting avenue for the treatment of...
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Discovery of diarylpyrimidine derivatives bearing piperazine sulfonyl as potent HIV-1 nonnucleoside reverse transcriptase inhibitors
HIV-1 reverse transcriptase is one of the most attractive targets for the treatment of AIDS. However, the rapid emergence of drug-resistant strains...
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Expression of soluble moloney murine leukemia virus-reverse transcriptase in Escherichia coli BL21 star (DE3) using autoinduction system
Autoinduction systems in Escherichia coli can control the production of proteins without the addition of a particular inducer. In the present study,...
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In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity
As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based...
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Analysis of 3′-Phosphoadenosine 5′-Phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse Transcription Polymerase Chain Reaction, and Immunohistochemistry
3′-Phosphoadenosine 5′-phosphosulfate transporters (PAPSTs) play an important role in transporting 3′-phosphoadenosine 5′-phosphosulfate (PAPS), the...
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Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses
Cassava brown streak disease (CBSD) caused by Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) is the most...
