Abstract
The eco-friendly and cost-effective strategies for the preparation of nanoparticles are the cutting edge researches in the field of nanotechnology. The objective of the study was the eco-friendly synthesis, characterization and their antibacterial activity of AgNPs using stem extract of Phyllanthus pinnatus. The appearance of the dark brown color showed the biofabrication of AgNPs and its characterised by UV–Vis, SEM, XRD, and FT-IR analysis. Consequently, the antibacterial activity of AgNPs was tested against different bacterial pathogens. The UV–Vis spectrum of colloidal AgNPs exhibited a strong absorption peak at 490 nm. Besides, the SEM image showed that the AgNPs were fairly uniform in the nanoscale with mostly cubical morphology. The XRD analysis showed the crystalline nature of AgNPs. In addition, the FT-IR spectrum revealed the presence of functional groups attached to the surface of AgNPs that may have a role in bioreduction and stabilization of AgNPs. The AgNPs showed dose-dependent toxicity against bacterial pathogens. The maximum zone of inhibition was found at 1.8 mm in diameter for both Vibrio cholera and Shigella flexneri at 40 µL concentration. Although the current study showed potent in vitro antibacterial activity of AgNPs, further studies are required to establish the bactericidal potential of AgNPs on animal models for drug development.
Similar content being viewed by others
Change history
31 March 2020
In the original publication of this article, co-author name was missed to include in the author group.
References
A. Albanese, P. S. Tang, and W. C. W. Chan (2012). Annu. Rev. Biomed. Eng.14, 1.
M. Fakruddin, Z. Hossain, and H. Afroz (2012). J. Nanobiotechnol.10, 31.
N. Karimi, A. Chardoli, and A. Fattahi (2017). Iran. J. Pharm. Res.16, 1167.
H. Barabadi, M. A. Mahjoub, B. Tajani, A. Ahmadi, Y. Junejo, and M. Saravanan (2019). J. Clust. Sci.30, 259.
P. Boomi, G. P. Poorani, S. Palanisamy, S. Selvam, G. Ramanathan, S. Ravikumar, H. Barabadi, H. G. Prabu, J. Jeyakanthan, and M. Saravanan (2019). J. Clust. Sci. https://doi.org/10.1007/s10876-019-0153.
H. Barabadi (2017). Cell. Mol. Biol.63, 3.
S. Salari, S. Esmaeilzadeh Bahabadi, A. Samzadeh-Kermani, and F. Yousefzaei (2019). Iran. J. Pharm. Res.18, 430.
L. Aghebati-maleki, B. Salehi, R. Behfar, H. Saeidmanesh, F. Ahmadian, M. Sarebanhassanabadi, and M. Negahdary (2014). Int. J. Electrochem. Sci.9, 257.
T. Ramezani, M. Nabiuni, J. Baharara, K. Parivar, and F. Namvar (2019). Iran. J. Pharm. Res.18, 222.
H. Barabadi, M. Ovais, Z. K. Shinwari, and M. Saravanan (2017). Green Chem. Lett. Rev.10, 285.
M. Ovais, A. T. Khalil, A. Raza, M. A. Khan, I. Ahmad, N. U. Islam, M. Saravanan, M. F. Ubaid, M. Ali, and Z. K. Shinwari (2016). Nanomedicine.11, 3157.
M. Maham and R. Karami-Osboo (2017). Iran. J. Pharm. Res.16, 462.
M. Kasithevar, M. Saravanan, P. Prakash, H. Kumar, M. Ovais, H. Barabadi, and Z. K. Shinwari (2017). J. Interdiscip. Nanomed.2, 131.
S. A. Dahoumane, M. Mechouet, K. Wijesekera, C. D. M. Filipe, C. Sicard, D. A. Bazylinski, and C. Jeffryes (2017). Green Chem.19, 552.
A. Arya, K. Gupta, T. S. Chundawat, and D. Vaya (2018). Bioinorg. Chem. Appl.2018, 7879403.
Z. Rezvani Amin, Z. Khashyarmanesh, B. S. Fazly Bazzaz, and Z. Sabeti Noghabi (2019). Iran. J. Pharm. Res.18, 210.
M. Saravanan, S. K. Barik, D. MubarakAli, P. Prakash, and A. Pugazhendhi (2018). Microb. Pathog.116, 221.
M. Manimaran and K. Kannabiran (2017). Lett. Appl. Microbiol.64, 401.
F. Niknejad, M. Nabili, R. Daie Ghazvini, and M. Moazeni (2015). Curr. Med. Mycol.1, 17.
H. Barabadi and S. Honary (2016). Pharm. Biomed. Res.2, 1.
H. Barabadi and S. Honary (2014). Razi J. Med. Sci.21, 20.
H. Barabadi, A. Alizadeh, M. Ovais, A. Ahmadi, Z. K. Shinwari, and M. Saravanan (2018). IET Nanobiotechnol.12, 377.
Q. Abbas, M. Saleem, A. R. Phull, M. Rafiq, M. Hassan, K.-H. Lee, and S.-Y. Seo (2017). Iran. J. Pharm. Res.16, 760.
H. Barabadi, S. Honary, P. Ebrahimi, A. Alizadeh, F. Naghibi, and M. Saravanan (2019). Inorg. Nano Metals Chem. https://doi.org/10.1080/24701556.2019.1583251.
R. Subbaiya, M. Saravanan, A. R. Priya, K. R. Shankar, M. Selvam, M. Ovais, R. Balajee, and H. Barabadi (2017). IET Nanobiotechnol.11, 965.
S. Honary, H. Barabadi, E. Gharaei-Fathabad, and F. Naghibi (2013). Trop. J. Pharm. Res.12, 7.
H. Barabadi, S. Honary, M. Ali Mohammadi, E. Ahmadpour, M. T. Rahimi, A. Alizadeh, F. Naghibi, and M. Saravanan (2017). Environ. Sci. Pollut. Res. Int.24, 5800.
S. Honary, E. Gharaei-Fathabad, H. Barabadi, and F. Naghibi (2013). J. Nanosci. Nanotechnol.13, 1427.
H. Barabadi, S. Honary, P. Ebrahimi, M. A. Mohammadi, A. Alizadeh, and F. Naghibi (2014). Braz. J. Microbiol.45, 1493.
S. Honary, H. Barabadi, E. Gharaei-Fathabad, and F. Naghibi (2012). Dig. J. Nanomater. Bios.7, 999.
R. Dobrucka (2017). Iran. J. Pharm. Res.16, 753.
S. Honary, H. Barabadi, P. Ebrahimi, F. Naghibi, and A. Alizadeh (2015). J. Nano Res.30, 106.
M. Saravanan, V. Gopinath, M. K. Chaurasia, A. Syed, F. Ameen, and N. Purushothaman (2018). Microb. Pathog.115, 57.
H. Barabadi, F. Kobarfard, and H. Vahidi (2018). Iran. J. Pharm. Res.17, 87.
M. Saravanan, T. Asmalash, A. Gebrekidan, D. Gebreegziabiher, T. Araya, H. Hilekiros, H. Barabadi, and K. Ramanathan (2018). Pharm Nanotechnol.6, 17.
J. A. John Paul, B. Karunai Selvi, and N. Karmegam (2015). Appl. Nanosci.5, 937.
K. Kanagamani, P. Muthukrishnan, K. Shankar, A. Kathiresan, H. Barabadi, and M. Saravanan (2019). J. Clust. Sci. https://doi.org/10.1007/s10876-019-01583-y.
P. Prakash, P. Gnanaprakasam, R. Emmanuel, S. Arokiyaraj, and M. Saravanan (2013). Colloids Surf. B Biointerfaces108, 255.
Y. Kuthati, R. K. Kankala, S. X. Lin, C. F. Weng, and C. H. Lee (2015). Mol. Pharm.12, 2289.
M. Ovais, A. T. Khalil, N. U. Islam, I. Ahmad, M. Ayaz, M. Saravanan, Z. K. Shinwari, and S. Mukherjee (2018). Appl. Microbiol. Biotechnol.102, 6799.
B. Sarin, N. Verma, J. P. Martín, and A. Mohanty (2014). Sci. World J.2014, 839172.
K. Singh, M. Panghal, S. Kadyan, U. Chaudhary, and J. P. Yadav (2014). J. Nanobiotechnol.12, 40.
P. Kathireswari, S. Gomathi, and K. Saminathan (2014). Int. J. Curr. Microbiol. Appl. Sci.3, 960.
M. M. Or Rashid, M. S. Islam, M. A. Haque, M. A. Rahman, M. T. Hossain, and M. A. Hamid (2016). Iran. J. Pharm. Res.15, 591.
M. Ovais, A. T. Khalil, A. Raza, N. U. Islam, M. Ayaz, M. Saravanan, M. Ali, I. Ahmad, M. Shahid, and Z. K. Shinwari (2018). Appl. Microbiol. Biotechnol.102, 4393.
M. Ovais, A. Raza, S. Naz, N. U. Islam, A. T. Khalil, S. Ali, M. A. Khan, and Z. K. Shinwari (2017). Appl. Microbiol. Biotechnol.101, 3551.
R. Emmanuel, M. Saravanan, M. Ovais, S. Padmavathy, Z. K. Shinwari, and P. Prakash (2017). Microb. Pathog.113, 295.
M. Anandan, G. Poorani, P. Boomi, K. Varunkumar, K. Anand, A. A. Chuturgoon, M. Saravanan, and H. Gurumallesh Prabu (2019). Process Biochem. https://doi.org/10.1016/j.procbio.2019.02.014.
M. Saravanan, S. Arokiyaraj, T. Lakshmi, and A. Pugazhendhi (2018). Microb. Pathog.117, 68.
X. Yan, B. He, L. Liu, G. Qu, J. Shi, L. Hu, and G. Jiang (2018). Metallomics10, 557.
Acknowledgements
The authors thankfully acknowledge the Sathyabama Institute of Science and Technology, Mekelle University, UGC [No.F.14-13/2013, Inno/ASIST], DST-FIST [SR/FST/LSI-667/2016(C)] and MHRD-RUSA 2.0 [F.24/51/2014-U, Policy (TNMulti-Gen), Dept. of Edn. Govt. of India] for the financial supports and infrastructure facilities.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Balachandar, R., Gurumoorthy, P., Karmegam, N. et al. Plant-Mediated Synthesis, Characterization and Bactericidal Potential of Emerging Silver Nanoparticles Using Stem Extract of Phyllanthus pinnatus: A Recent Advance in Phytonanotechnology. J Clust Sci 30, 1481–1488 (2019). https://doi.org/10.1007/s10876-019-01591-y
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10876-019-01591-y