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Structural analysis of a polysaccharide fromChlorella kessleri by means of gas chromatography—mass spectrometry of its saccharide alditols

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Abstract

Interpretation of gas chromatographic-mass spectrometric data of oligosaccharide alditols was used to determine their structures and to derive the structure of a water soluble polysaccharide isolated fromChlorella kessleri.1H- and13C-NMR was employed to assess the configuration of glycosidic bonds and individual monosaccharides were assigned to thel ord series by means of gas chromatography of the acetylated (S)-2-butyl glycosides.

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Abbreviations

GC:

gas chromatography

MS:

mass spectrometry

SIM:

simultaneously monitor(ed)

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Correspondence to T. Řezanka.

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The work was supported by the Research Concept of theInstitute of Microbiology AV 0Z 5020 0510.

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Řezanka, T., Sigler, K. Structural analysis of a polysaccharide fromChlorella kessleri by means of gas chromatography—mass spectrometry of its saccharide alditols. Folia Microbiol 52, 246–252 (2007). https://doi.org/10.1007/BF02931306

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  • DOI: https://doi.org/10.1007/BF02931306

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