Abstract
NVB333 is a novel semisynthetic lantibiotic derived from the amide coupling of 3,5-dichlorobenzylamine to the C-terminal of deoxyactagardine B. The in vitro activity of NVB333 includes efficacy against clinically relevant pathogens including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus spp. NVB333 shows no cross-resistance with other antibiotics tested and a very low propensity for resistance development. After intravenous dosing NVB333 has high exposure in mouse plasma and shows generally improved in vivo activity compared with vancomycin in mouse infection models despite modest MIC values. In thigh infection models, promising efficacy was demonstrated against several strains of S. aureus including methicillin-resistant S. aureus (MRSA) and vancomycin-intermediate S. aureus (VISA) strains, and against Enterococcus faecalis UNT126-3. Area under the concentration curve (AUC)/MIC was shown to be the best predictor of efficacy against S. aureus UNT103-3 with an AUC/MIC of 138 (uncorrected for protein binding) achieving a static effect. NVB333 was also effective in a disseminated infection model where it conferred complete survival from the MRSA strain ATCC 33591. NVB333 showed rather modest lung penetration after intravenous dosing (AUC in lung 2–3% of plasma AUC), but because of very high plasma exposure, therapeutic levels of compound were achieved in the lung. Efficacy at least equal to vancomycin was demonstrated against an MRSA strain (UNT084-3) in a bronchoalveolar infection model. The impressive in vivo efficacy of NVB333 and strong resistance prognosis makes this compound an interesting candidate for development for treating systemic Gram-positive infections.
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Introduction
A recent report by the World Health Organization1 has further highlighted the increasing urgency to find a solution to the global problem of a dwindling antimicrobial armamentarium in the face of increasing prevalence of resistance to antibiotics. Estimates suggest that without new antibiotics, by 2050 global annual mortality rates could rise from current levels of ∼700 000 to 10 million as a result of the widening spread of antimicrobial resistance.2 In addition to the implications to health, the global economic impact of increasing resistance could be up to 100 trillion US dollars by 2050.2 Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus spp. are among the main protagonists.
The launch of government initiatives such as GAIN (Generating Antibiotics Incentives Now; Food and Drug Administration (FDA) Safety and Innovation Act, 2012) may have helped stimulate recent FDA approvals for antibiotics (4 approved in 20143 compared with 22 from 2000 to the end of 20124); nevertheless, there remains a real dearth of agents from new classes offering novel mechanisms of action and the potential for reduced resistance development. Although alternative approaches to antibiotics in the treatment of bacterial infections are gaining momentum,5 the search for new improved antibiotics remains an important area of exploration. Antimicrobial peptides including lantibiotics represent a largely untapped potential source of antibiotics that crucially may offer a promising resistance prognosis.
Lantibiotics are ribosomally synthesized post-translationally modified peptides that have encouraging activity predominantly against clinically important Gram-positive pathogens.6 The type B lantibiotics that include mersacidin, actagardine and deoxyactagardine B (DAB7) are globular and exert their antibacterial effect by inhibition of cell wall biosynthesis.8 Lipid II is not the product of a single gene but derived from multiple enzyme-catalyzed reactions, and as such agents targeting lipid II have a reduced propensity for resistance development as recently described for the antibacterial teixobactin.9 Unlike glycopeptides these lantibiotics do not bind to the D-Ala-D-Ala motif of lipid II, but to the pyrophosphate-sugar moiety.10 Importantly this alternative target site reduces the chance of cross-resistance with vancomycin-resistant organisms and increases the attractiveness of lantibiotics as medically useful molecules.
To date, only three lantibiotics, NAI-107,11 Mu114012 and NVB302,13 have advanced through formal preclinical development and/or clinical trials as antibacterial agents. We report here the evaluation of NVB333, a novel semisynthetic lantibiotic, for efficacy in mouse thigh, lung and disseminated infection models with clinically relevant pathogens. Vancomycin has been included as a comparator alongside NVB333 as it remains the most commonly used narrow spectrum agent to treat Gram-positive infections.
Materials and methods
Bacterial strains and antibacterials
The bacterial strains used in the in vitro determination of MICs were collected from global surveillance studies (Eurofins Medinet, Chantilly, VA, USA) and were selected to be representative of those encountered clinically in the United States and Europe. These strains also included resistant populations (for example, daptomycin and linezolid nonsusceptible isolates) of staphylococci and enterococci. Infections were established in in vivo models using bacteria sourced from the American Type Culture Collection (ATCC, Manassas, VA, USA) or from the University of North Texas Health Science Center (UNTHSC, Fort Worth, TX, USA; UNT prefix). These strains included MRSA strains with MIC values that represent the MIC50 (4 mg l−1) and MIC90 (8 mg l−1) values determined for NVB333. The S. aureus isolates UNT084-3 and UNT103-3 are both USA100 MRSA genotype, whereas UNT107-3 and UNT108-3 are vancomycin-intermediate S. aureus (VISA; MIC vancomycin 4–8 mg l−1) isolates. UNT126-1 is a vancomycin-resistant Enterococcus faecalis (MIC vancomycin >8 mg l−1).
NVB333 is the C-terminal 3,5-dichlorobenzylamine monocarboxamide derivative of the natural product DAB that was isolated from fermentation broth of the actinomycete Actinoplanes liguriae.7 The N-methyl-D-glucamine salt form of NVB333 was supplied by Novacta Biosystems (Welwyn Garden City, UK) and used for all studies. This salt form is soluble in water at a concentration of at least 50 mg ml−1. It was dissolved in 5% sorbitol and pH adjusted to pH 8.0 to 8.5 with HCl for dosing in the thigh and lung infection models. Vancomycin was from commercial sources and prepared in saline for thigh and lung infection studies. Both NVB333 and vancomycin were prepared in 15% 2-hydroxypropyl-β-cyclodextrin/4.4% glucose/0.5 mM KH2PO4, pH 5.0, for the disseminated infection study.
In vitro methods
MICs were determined by microbroth dilution using cation-adjusted Mueller-Hinton broth in 96-well microtiter plates according to Clinical and Laboratory Standards Institute (CLSI) guidelines.14
Animals
Female specific-pathogen-free CD-1 mice were used in thigh and lung infection models, whereas male CD-1 mice were used in the disseminated infection model. All mice weighed ∼22±2 g. For thigh and lung models, neutropenia was induced by i.p. injection of cyclophosphamide at 150 mg kg−1 4 days before infection and 100 mg kg−1 1 day before infection. Previous studies have shown that this produces neutropenia in this model for 5 days.15 All animal studies were performed with local ethical committee clearance following protocols approved by the institutional animal care and use committee.
Disseminated infection model
Groups of six mice (immunocompetent) were inoculated i.p. with S. aureus MRSA ATCC 33591 (1.1 × 107 CFUs per mouse) suspended in 0.5 ml of Brain Heart Infusion Broth (Oxoid, Basingstoke, UK) with 5% mucin.16 Test substances were administered s.c. at doses of 1, 3, 5, 10 and 20 mg kg−1 immediately after bacterial challenge and then at 2 and 24 h after infection. A control group of infected mice was dosed in parallel receiving vehicle only. All 6 of the animals from this group had died within 48 h of infection. Mortality was recorded once daily for 7 days and the ED50 determined at the end of the 7-day period by nonlinear regression using GraphPad Prism (GraphPad software, La Jolla, CA, USA).
Mouse thigh infection model
Neutropenic mice were infected by i.m. injection into the right lateral thigh muscle. Each thigh received ~1 × 105 CFUs (0.1 ml per thigh). Groups of 5 mice were administered test article by i.v. injection at 2 and 14 h after infection. At 2 h after infection, a control group of animals was humanely killed using CO2 inhalation to provide a pretreatment control group (start group). At 26 h after infection, all remaining groups were humanely killed by CO2 inhalation. The right thigh muscle from each animal was removed and homogenized in ice-cold sterile phosphate-buffered saline, pH 7.4, using a Polytron tissue homogenizer (Kinematica AG, Lucerne, Switzerland). The homogenates were then quantitatively cultured onto Brain Heart Infusion agar (Oxoid) including 0.5% charcoal (w/v) and incubated at 37 °C for 18–24 h before colonies were enumerated.17
Mouse lung infection model
Groups of five anesthetized (i.p. injection of 40 mg kg−1 ketamine/6 mg kg−1 xylazine mixture) neutropenic mice per treatment were infected by i.n. instillation of 50 μl inoculum into each nostril (5.62 log10 CFUs per mouse) and kept in an upright position for ~10 min after infection. Test agents were administered by i.v. injection at 2 and 14 h (b.i.d.) after infection. At 2 h after infection, a control group of animals was humanely killed by CO2 inhalation (start group). All remaining animals were killed in the same manner at 26 h after infection, including a group that had received vehicle only (end group). Lungs were aseptically removed and then homogenized and colonies enumerated per lung pair by plating dilutions onto Brain Heart Infusion agar including 0.5% charcoal (w/v). Plates were incubated at 37 °C overnight (~16–20 h) before colonies were enumerated.
Mouse lung pharmacokinetics
The levels of NVB333 in both plasma and epithelial lining fluid (ELF) were determined in neutropenic mice infected by i.n. instillation using an inoculum of 5 log10 CFU MRSA UNT084-3. At 2 h after infection, NVB333 was administered i.v. at dose levels of 25 and 80 mg kg−1. Before dosing and then at 0.083, 0.25, 0.5, 1, 2, 4 and 8 h after dosing, groups of three mice per timepoint were killed by CO2 inhalation and blood collected by cardiac puncture. ELF was recovered from the lungs of each animal by washing up to three times (bronchoalveolar lavage) with 1 ml sterile saline using a 1 ml tuberculin syringe. The levels of NVB333 in both plasma and ELF were determined by LC-MS. Urea concentrations in both the blood and ELF samples were determined using a Quantichrom Urea Assay Kit (Bioassay Systems, Hayward, CA, USA) to ascertain the dilution factor of the ELF during the saline washes. Concentrations of NVB333 in the ELF were calculated using the formula; ELF concentration=(AC × BU)/LU, where AC is the antibiotic concentration in the bronchoalveolar lavage, BU is the blood urea concentration and LU is the lavage fluid urea concentration. An additional set of infected animals was killed and lungs removed to verify the level of bacterial titers at 2 and 8 h after infection
Pharmacokinetic/pharmacodynamic (PK/PD) relationship of NVB333
The PK/PD relationship for NVB333 was investigated using the mouse thigh infection model and the MRSA strain UNT103-3.
Single-dose pharmacokinetics of NVB333 were determined in thigh-infected mice at dose levels of 5, 10, 25, 50 and 80 mg kg−1. At 2 h after infection, mice received NVB333 by s.c. administration, and then at 0.083, 0.25, 0.5, 1, 2, 4, 8 and 24 h after drug administration, groups of 3 animals for each dose level were killed. Blood was collected by cardiac puncture and allowed to clot on ice. Serum was separated from the clot by centrifugation and the concentration of NVB333 determined by LC-MS. The PK parameters were determined by noncompartmental methods using PK Solutions 2.0 (Summit Research Services, Montrose, CO, USA).
Dose range studies for NVB333 were followed by dose fractionation studies. NVB333 was administered as a single s.c. dose at levels of 1, 5, 10, 20, 25, 40, 50, 80 and 100 mg kg−1 into thigh-infected mice at 2 h after infection. At 26 h, the mice were killed and the thighs removed and bacterial counts enumerated as described earlier. Total dose levels of 5, 10, 15, 20, 30, 50, 80 and 100 mg kg−1 were subsequently administered as fractionated doses over a 24-h period to infected mice beginning 2 h after infection. The dose levels were administered as either single injections, two equally divided doses given 12 h apart or four equally divided doses given 6 h apart. At 26 h after infection, thighs were removed and bacterial counts enumerated as before. A sigmoid dose–response model derived from the four-parameter Hill equation was used to calculate the respective relationship that produced a net bacteriostatic effect and a 1 log10 kill over 24 h (static and 1 log efficacy).
Sample and statistical analysis
Plasma samples were prepared for analysis by LC-MS by adding 150 μl of acetonitrile containing 0.1% (v/v) formic acid to 50 μl of plasma and mixing. Supernatant (75 μl) was then diluted with 150 μl of water before analysis. Samples (10 μl) were injected onto a Synergi Polar-RP column (2.5 μm 50 × 2.0 mm) on an Agilent G1312B HPLC linked to an Agilent 6410 mass spectrometer (Santa Clara, CA, USA). A gradient of 5 to 95% acetonitrile (0.1% formic acid) over 5 min was used with a flow rate of 0.5 ml min−1.
Counts of bacterial colonies recovered from homogenized tissue from efficacy studies were compared by one-way analysis of variance and Dunnett’s multiple comparison test against the end control using GraphPad Prism version 6.05. No significant difference between groups: *P<0.05.
Results
In vitro antibacterial activity
The MICs of NVB333 and vancomycin were determined against a panel of organisms that consist of a representative population of recent clinical isolates of Gram-positive pathogens and are presented in Table 1. The MIC values for NVB333 against vancomycin-susceptible strains were generally at least fourfold higher than for vancomycin. An exception was Streptococcus pyogenes against which NVB333 had an MIC90 of ⩽0.125 mg l−1 compared with 0.5 mg l−1 for vancomycin, although it should be noted that a small number of strains had MIC values for NVB333 as high as 4 mg l−1.
It was notable that MIC values for NVB333 were unaffected by resistance to vancomycin and linezolid, although a slight (2 × ) elevation of MIC value was observed for VISA and daptomycin resistant strains. This ‘nonspecific’ MIC elevation is common to cell wall-active antibiotics.18
Disseminated lethal infection model
As an initial indication of the efficacy of NVB333 in protecting mice from a disseminated infection, NVB333 was administered to groups of six mice infected with S. aureus ATCC 33591. The MIC of NVB333 against this MRSA strain was 2 mg l−1, and this is below the MIC50 value determined for NVB333. Infected mice received NVB333 or vancomycin as a comparator delivered s.c. immediately before infection and then at 2 and 24 h after infection and mortality was then recorded once daily for 7 days. At the end of this period, the ED50 values or concentrations required to achieve 50% survival compared with the vehicle control were determined for NVB333 and vancomycin as 1 and 3 mg kg−1, respectively.
Efficacy against S. aureus infection in the neutropenic mouse thigh infection model
In order to investigate more quantitatively the efficacy of i.v. NVB333, NVB333 was administered i.v. in a series of neutropenic mouse thigh infection studies where groups of five mice had been infected with one of four strains of S. aureus. Vancomycin, which is commonly used to treat systemic Gram-positive infections, was dosed alongside NVB333 as a comparator. The test agents were dosed i.v. as this is the most direct route of administration and removes the risk that comparisons between compounds will be compromised by differences in release kinetics from a s.c. compartment.
The strains used in these studies demonstrated different levels of in vitro susceptibility to NVB333 and vancomycin. The MIC of NVB333 against the MRSA strain ATCC 33591 was 2 mg l−1; the other strains that were evaluated in the infection models were the MRSA strain UNT103-3 for which the MIC of NVB333 was 4 mg l−1, equivalent to the MIC50 for NVB333, and the two VISA strains UNT107-3 and UNT108-3 for which the MIC of NVB333 was 8 mg l−1, equivalent to the MIC90 for NVB333. The MIC for vancomycin was 4 mg l−1 against the VISA strains and 1 mg l−1 for all other strains. The effect of i.v. administration of NVB333 and vancomycin on the bacterial burden of neutropenic CD-1 mice infected in the lateral thigh muscle with these strains of S. aureus is shown in Figure 1.
Thigh infection was established with all four isolates with the 2 h control groups (start) having a bacterial burden of ∼5 log10 CFU/thigh that increased to between 7 and 9 log10 CFU/thigh after 26 h (end) in the untreated controls. In all studies, both NVB333 and vancomycin were administered i.v. at 2 and 14 h after infection, with harvest of the thigh tissue at 26 h after infection.
Both NVB333 and vancomycin showed a dose-dependent reduction in counts. The dose required to achieve the static dose and 1 log10 CFU reduction below the static dose is shown in Table 2. As expected for NVB333, this dose tended to increase with the MIC of the strain.
Efficacy against E. faecalis (vancomycin-resistant enterococcus) infection in the neutropenic mouse thigh infection model
The efficacy of NVB333 was also evaluated against E. faecalis UNT126-1. This strain of E. faecalis is vancomycin resistant (MIC >8 mg l−1), with the MIC of NVB333 being 4 mg l−1. Both NVB333 and vancomycin were administered i.v. at 2 and 14 h after infection with harvest of the thigh tissue at 26 h after infection. The mean bacterial burdens of the control and treatment groups are shown in Figure 2. The start and end control groups had bacterial burdens of 5.58 and 7.13 log10 CFU/thigh, respectively.
The lowest dose of NVB333 tested (10 mg kg−1 × 2) caused a reduction in counts below the static level. Vancomycin appeared to be borderline for efficacy with 100 mg kg−1 × 2 needed to show a comparable effect. The reduction in bacterial burden of this strain of E. faecalis in the thigh model was comparable to the response of the MRSA strain for which the MIC of NVB333 is the same (Table 2).
Mouse lung infection model
The efficacy of NVB333 and vancomycin was determined in a mouse lung infection model with compounds delivered i.v. at 2 and 14 h after infection with harvest of the lung tissue at 26 h after infection. In this model the S. aureus MRSA UNT084-3 strain was used to establish an infection in the lungs. The bacterial burden of the control group at the first dosing timepoint (2 h after infection) was 7.23 log10 CFUs. This had increased to 8.83 log10 CFUs in the 26 h control (end) group. The i.v. administration of NVB333 at dose levels of 10 to 80 mg kg−1 resulted in a maximum reduction in bacterial burden of 1.59 log10 CFUs compared with the 26 h control group that was close to a static effect. The response to the administration of vancomycin across the dose levels was less marked with a maximum reduction in bacterial burden of 0.25 log10 CFUs at 80 mg kg−1 compared with the vehicle control. The reduction in bacterial burdens across the complete range of dose levels is shown in Figure 3, and the dose levels required to reach 1 log10 reduction below the 24 h control (end) are shown in Table 2.
The PK of NVB333 in the plasma and ELF were determined using a separate cohort of infected mice. NVB333 was administered i.v. at single dose levels of 25 and 80 mg kg−1 2 h after infection and plasma and ELF were collected at 7 timepoints to 8 h after dosing. The PK parameters determined from measurements of total amounts of NVB333 in the plasma and ELF are shown in Table 3. Overall, plasma exposure (area under the concentration curve (AUC)) was dose proportional with an AUCinf of 992.3 μg h ml−1 at 25 mg kg−1 and 3101 μg h ml−1 at 80 mg kg−1. The plasma half-life of 3.2 and 3.3 h, clearance values of 25.2 and 25.8 ml h−1 kg−1 and volume of distribution of 115.3 and 123.7 ml kg−1 were comparable for the 25 and 80 mg kg−1 doses. Peak ELF levels of NVB333 reached 5.06 μg ml−1 at 0.5 h for the 25 mg kg−1 dose and 15.31 μg ml−1 at 2 h for the 80 mg kg−1 dose. The half-life of NVB333 in the ELF appeared to be comparable to plasma (2.1–3.7 h). Total ELF exposure was 33 μg h ml−1 at 25 mg kg−1 and 60.3 μg h ml−1 at 80 mg kg−1. This represents ∼2–3% penetration into the ELF (based on AUC) as compared with exposure in plasma. The low penetration of NVB333 into the ELF may be a result of high protein binding of NVB333. Despite this the levels of NVB333 in ELF remain above the MIC for substantial periods.
Relationship between PK/PD indices and antibacterial activity in the mouse thigh infection model
The PK/PD relationship of NVB333 in the mouse thigh infection model was determined for S. aureus MRSA UNT103-3. The MIC of NVB333 against this organism was 4 mg l−1, equivalent to the MIC50 for NVB333 against S. aureus. In order to facilitate dosing more than three times per day and mitigate issues associated with multiple i.v. dosing of animals in large numbers, NVB333 was dosed s.c. The total (that is, free plus protein bound) plasma concentrations of NVB333 in neutropenic mice infected in the thigh with S. aureus MRSA UNT103-3 as a function of time after single SC doses of NVB333 of 5, 10, 25, 50 and 80 mg kg−1 are shown in Figure 4. Peak plasma levels of NVB333 dosed s.c. were achieved at 2 to 8 h after administration. Cmax levels ranged from 17.8 μg ml−1 at 5 mg kg−1 to 68.7 μg ml−1 at 80 mg kg−1. Total plasma exposure (AUC) was dose proportional with calculated values of 88.7 μg h−1 ml−1 at 5 mg kg−1 increasing to 1434.7 μg h−1 ml−1 at 80 mg kg−1.
Two dose ranging efficacy studies were then carried out. The first resulted in control counts at 2 h of 5.75±0.15 log10 CFUs that increased to 7.95±0.30 log10 CFUs at 26 h, whereas the second study exhibited counts of 5.29±0.83 log10 CFUs at 2 h and 9.02±0.15 log10 CFUs for the 26 h control. In both studies treatment with NVB333 demonstrated a similar dose response; in the first study the doses of 25 to 100 mg kg−1 resulted in a 2.37 to 3.16 log10 CFU reduction from the 26 h control counts, whereas in the second study doses of 20 to 100 mg kg−1 produced log10 CFU reductions of 1.11 to 3.94 log10 CFUs compared with the 26 h control.
In dose fractionation studies, NVB333 was delivered to infected animals at total doses of 5, 10, 15, 20, 30, 50, 80 and 100 mg kg−1 administered as a single dose (q24h) 2 h after infection or as two equal doses (q12h) administered 12 h apart or four equal doses delivered 6 h apart (q6h). The dosage required to achieve a reduction of the bacterial burden to the static dose level was 31.8, 25.4 and 38.8 mg kg−1 for the q24h, q12h and q6h regimens, respectively. The log10 CFU counts from the dose fractionation study were plotted against PK/PD parameters of AUC/MIC, Cmax/MIC and %T24>MIC and are shown in Figure 5. The PK/PD parameter that correlated closest with efficacy was AUC/MIC with a correlation coefficient of r2=0.90, followed by Cmax/MIC (r2=0.85) and %T24>MIC (r2=0.57). The magnitudes of these parameters required to achieve a static effect in this model were an AUC/MIC of 138, Cmax/MIC of 8.1 and %T24>MIC of 95.9%. The magnitude required for a 1 log10 CFU reduction from the static dose was calculated at 234 for AUC/MIC and 13.8 for Cmax/MIC. It is important to note that these calculations are based on total drug levels and not free unbound NVB333. It proved difficult to determine protein binding reliably using a rapid equilibrium device because of nonspecific binding; however, it is estimated that NVB333 was ⩾90% protein bound.
Discussion
The global surge of resistant pathogenic bacteria and growth in the identification of new mechanisms that confer resistance against current antibacterial agents emphasizes the need for new antibacterials particularly with novel mechanisms of action. Despite this the vast majority of many of the estimated 39 new antibiotics believed to be in clinical development19 represent modified versions of approved antibiotics. In attempts to widen the pool of potential novel antibacterials, attention has increasingly turned to alternative and perhaps previously overlooked classes of molecules, and among them the lantibiotics.6
NVB333 is the C-terminal 3,5-dichlorobenzylamine monocarboxamide derivative of the lantibiotic DAB. The compound emerged from a medicinal chemistry program aimed primarily at increasing the antibacterial activity of DAB against staphylococci and enterococci. NVB302, the diaminoheptane monocarboxamide C-terminal derivative of DAB, emerged from a similar program aiming to enhance activity versus Clostridium difficile and is currently under development for the treatment of C. difficile infection.20 The antibacterial activity of NVB333 in vitro spans clinically relevant Gram-positive pathogens. Importantly, NVB333 retains its activity against strains with resistance to other important anti-MRSA antibiotics including vancomycin, daptomycin and linezolid as well as penicillin-resistant Streptococcus pneumoniae (data not shown). In addition, no spontaneous resistant isolates were detected when up to 1011 CFUs of strains of S. aureus MRSA (strain R33, Health Protection Agency) and MSSA (strain SH1000 21), E. faecium ATCC 19579 (VSE) and S. pyogenes (clinical isolate, University of Leeds, Leeds general infirmary) were plated onto agar containing four times the respective MIC level of NVB333 (data not shown). This lack of resistance development is consistent with the assumed mode of action (lipid II binding) as established for actagardine.10
The modest in vitro activity of NVB333 belies its effectiveness in vivo as indeed has previously been noted for both mersacidin22 and actagardine.23 In a disseminated infection model, the ED50 value for survival over the course of 7 days was three times lower for NVB333 than required for vancomycin (despite the lower MIC of the latter). The strains of S. aureus challenged in the thigh infection models cover a range of susceptibilities to NVB333 and include two VISA clinical isolates with MICs equal to the MIC90 for NVB333. Although in vitro NVB333 is two to four times less active than vancomycin against each of these strains, in vivo the efficacy of NVB333 is comparable or greater than vancomycin, particularly in the case of the MRSA strains ATCC 33591 and UNT103-3. NVB333 was also active against a vancomycin-resistant E. faecalis in the thigh model at a similar dose as required for efficacy against S. aureus.
The PK/PD analysis of the thigh infection model (S. aureus UNT103-3) was determined through dose fractionation studies of NVB333. Administration was through the s.c. route that enabled multiple dosing of animals over a 24-h period. The PK parameters of NVB333 measured following s.c. administration show a delay and reduction in Cmax compared with i.v. dosing. The fractionation studies revealed that AUC/MIC was the index most predictive of the in vivo antibacterial effect of NVB333. AUC/MIC is the same PK/PD driver reported for daptomycin, vancomycin and NAI-107. Reported values of AUC/MIC (total drug) required to deliver a static effect for these three drugs were in a similar range that is, 169 to 608 (daptomycin24), 33 to 212 (vancomycin24) and 371 (NAI-107, mean level from multiple strains25), respectively. The AUC/MIC (total drug) of NVB333 required to achieve stasis was measured as 138. In the lung infection model NVB333 was more effective than vancomycin, reducing bacterial burdens to lower levels than vancomycin at comparable mg kg−1 dose levels. Measurements of NVB333 in the ELF of infected lungs suggest the level of exposure is only ∼2–3% of the plasma exposure (AUC) possibly because of the high protein binding of NVB333. However, because of the high plasma exposure this still represents useful therapeutic levels of NVB333 in the lung.
In all in vivo studies NVB333 was well tolerated at the dose levels used and there were no signs of any drug-related adverse effects. NVB333 has demonstrated comparable or greater activity than vancomycin at comparable dose levels (mg kg−1) in in vivo models against clinically relevant Gram-positive pathogens. In a period when the effectiveness of current antibacterial agents is increasingly challenged globally, NVB333 represents a novel class of antibacterial that warrants further investigation.
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Acknowledgements
We thank MDS Pharma Services/Ricerca (now Eurofins Panlabs) for running the disseminated infection model and mouse thigh study using MRSA ATCC 33591. All other in vivo studies were carried out at the University of North Texas Health Science Center (UNTHSC). We also thank Eurofins Medinet for in vitro services. This work was partly supported by an R&D Development grant (no. 5496) awarded to Novacta Biosystem from the UK government.
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SB and MJD are shareholders of Novacta Biosystems, and MJD is on the board of directors for both Novacta Biosystems and Cantab Anti-infectives.
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Boakes, S., Weiss, W., Vinson, M. et al. Antibacterial activity of the novel semisynthetic lantibiotic NVB333 in vitro and in experimental infection models. J Antibiot 69, 850–857 (2016). https://doi.org/10.1038/ja.2016.47
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DOI: https://doi.org/10.1038/ja.2016.47
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