Abstract
Main Conclusion
The MUTE promoter contains a 175-bp region rich in Dof regulatory elements (AAAG) that is necessary and sufficient for initiation of transcription in meristemoids and the stomatal lineage.
The molecular mechanism underlying the decision to divide or differentiate is a central question in developmental biology. During stomatal development, expression of the master regulator MUTE triggers the differentiation of meristemoids into stomata. In this study, we carried out MUTE promoter deletion analysis to define a regulatory region that promotes the initiation of expression in meristemoids. Expression constructs with truncated promoter fragments fused to β-glucuronidase (GUS) were developed. The full-length promoter and promoter truncations of at least 500 bp from the translational start site exhibited normal spatiotemporal expression patterns. Further truncation revealed a 175-bp promoter fragment that was necessary and sufficient for stomatal-lineage expression. Known cis-elements were identified and tested for functional relevance. Comparison of orthologous MUTE promoters suggested DNA binding with one finger (Dof) regulatory elements and novel motifs may be important for regulation. Our data highlight the complexity and combinatorial control of gene regulation and provides tools to further investigate the genetic control of stomatal development.
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Introduction
Stem cells are essential for the production of cellular and tissue diversity across all organisms. Stem cells have the ability to divide asymmetrically to produce two daughter cells with different fates; one cell retains stem-cell characteristics, while the other may differentiate (Aichinger et al. 2012). In plants, several cell types outside major stem-cell niches have permanent or transient stem-cell characteristics (Fisher and Turner 2007; Pillitteri et al. 2007; Miyashima et al. 2013). Stomatal meristemoids are one such cell type. Stomata are epidermal structures composed of two guard cells that surround an intervening pore, where the size of the pore can be regulated based on turgor-driven changes in each guard cell. In dicotyledonous plants, the production of a stoma initiates with an unequal division of a meristemoid mother cell (MMC), which produces a meristemoid and a larger sister cell called a stomatal lineage ground cell (SLGC) (Fig. 1a). The SLGC can differentiate into a lobed epidermal cell or divide again to produce an additional meristemoid. A meristemoid can self-renew through repeated divisions (up to 3 additional times) or differentiate into a guard mother cell (GMC). The GMC undergoes one symmetric division to produce two terminally differentiated guard cells (Vaten and Bergmann 2012; Pillitteri and Dong 2013).
The number of times a meristemoid divides prior to GMC differentiation is a plastic trait regulated by internal and external factors. Research over the past decade has revealed a prominent role of basic helix–loop–helix (bHLH) proteins in the differentiation of stomatal-lineage cell types. One of these proteins, MUTE, is required for the differentiation of meristemoids (Pillitteri et al. 2007). Loss-of-function MUTE plants are stomataless and produce meristemoids that continually divide without differentiation. Conversely, ectopic overexpression of MUTE results in the conversion of all epidermal cells into stomata. Consistent with its function, the promoter activity of MUTE is only observed in the stomatal lineage, where expression is activated in a subset of meristemoids that will differentiate into stomata (Fig. 1a, b) (Pillitteri et al. 2008). A close paralog of MUTE called SPEECHLESS (SPCH) has also been implicated in meristemoid differentiation. Weak spch mutants produce meristemoids that undergo fewer divisions and prematurely differentiate into a GMC (MacAlister et al. 2007; Lau et al. 2014). Two additional bHLH proteins, SCREAM (SCRM) and its paralog SCRM2 physically interact with MUTE and SPCH to regulate their respective functions (Kanaoka et al. 2008).
Investigation into the plasticity of meristemoid divisions led to the identification of several signal transduction genes that promote or inhibit stomatal production (Vaten and Bergmann 2012; Pillitteri and Dong 2013). For example, loss-of-function mutations in some ERECTA-family (ERf) leucine-rich repeat receptor-like kinases (LRR-RLK) result in a reduction in the number of meristemoid divisions prior to GMC differentiation (Shpak et al. 2005). Similarly, alterations in the expression of the LRR receptor-like protein, TOO MANY MOUTHS (TMM), the subtilisin protease, STOMATAL DENSITY AND DISTRIBUTION1 (SDD1), small peptide ligands, or several members of the YODA (YDA) MAP kinase cascade result in fewer meristemoid divisions than wild type (Berger and Altmann 2000; Geisler et al. 2000; Hara et al. 2007; Lampard et al. 2008; Hunt and Gray 2009; Gudesblat et al. 2012; Jewaria et al. 2013). These signaling components are thought to mediate hormonal and environmental signals to direct stomata production under diverse conditions (Kim et al. 2012; Balcerowicz et al. 2014). Direct links between these signaling pathways and SPCH expression have been demonstrated (Lampard et al. 2008; Gudesblat et al. 2012).
The molecular basis of expression of some stomatal regulatory components has been investigated and broad microarray analyses have revealed novel stomatal-lineage specific genes and potential DNA binding sites for some stomatal regulators (Chinnusamy et al. 2003; Lai et al. 2005; Ohashi-Ito and Bergmann 2006; Pillitteri et al. 2011; Hachez et al. 2011; Adrian et al. 2015). Transcriptional profiling of meristemoids provided insight into the broad characteristics of meristemoids (Pillitteri et al. 2011; Adrian et al. 2015). However, detailed information on the transcriptional elements that control gene activation in this cell type is lacking. Because meristemoids are capable of reiterative asymmetric cell division, they are useful tools for investigating the molecular basis of stem-cell character. This work reports a detailed characterization of the promoter region of MUTE. Deletion analysis of the MUTE promoter led to the identification of a small 175-bp region that is necessary and sufficient to drive stomatal-lineage specific expression. In silico analysis revealed the presence of conserved DNA binding with one finger (Dof) binding elements within this region in orthologous genes, suggesting they may be important for regulation. Together, these findings further our understanding of the molecular mechanisms involved in gene regulation and provide tools to identify specific proteins that bind the MUTE promoter and regulate the differentiation of meristemoids.
Materials and methods
Plant transformation and plant growth conditions
Arabidopsis thaliana ecotype Columbia (Col) were used for transformation. Plants were grown under a 16 h/8 h light/dark cycle at 22–24 °C. All transformation constructs were transferred to Agrobacterium tumefaciens strain GV3101 by electroporation. Transformation of Arabidopsis was done by Agrobacterium-mediated transformation using floral dip method (Clough and Bent 1998). Seeds from all lines were surface sterilized with 30 % (v/v) bleach for 10 min. Transformants were selected by plating seeds on 0.5 MS media containing the appropriate antibiotic for plant selection and timentin (50 µg/ml). Positive transformants were confirmed by polymerase chain reaction (PCR) genotyping. Homozygous lines were confirmed by segregation ratio and used for analysis.
5′ and 3′-rapid amplification of cDNA ends (RACE)
Total RNA was extracted from 10-day-old Col seedlings using Qiagen RNeasy kit according to manufacturer’s instructions. 1 µg of total RNA was subjected to 5′ RACE with the GeneRacer Kit (Invitrogen) according to manufacturer’s instructions. Briefly, total RNA was dephosphorylated with calf intestinal phosphatase to eliminate RNAs that were not full length. Dephosphorylation with tobacco acid pyrophosphatase was used to remove the cap structure of full-length mRNAs and the GeneRacer RNA oligo was ligated to the 5′ end of the transcript. The RNA was reverse transcribed using the GeneRacer oligo dT primer and Superscript II Reverse Transcriptase. Nested PCR was used for both 5′ and 3′ end amplification. Nested PCR conditions used for amplification were as follows: (96 °C, 2 min); 5 cycles at (96 °C, 20 s; 72 °C, 30 s; 72 °C, 30 s); 5 cycles at (95 °C, 20 s; 70 °C, 30 s; 72 °C, 30 s) and 23 cycles at (95 °C, 20 s; 68 °C, 30 s; 72 °C, 30 s) using the standard primers from the kit and gene-specific primers listed in Suppl. Table S1. Amplified products were cloned into TOPO pCR4 TA cloning vector according to manufacturer’s instructions and sequenced using an M13F primer.
Vector construction
The full-length MUTE promoter designated in this study includes 1954 bp of promoter sequence immediately upstream of the translation initiation codon (Pillitteri et al. 2007). A series of progressive 5′-flanking region deletion fragments of the full-length promoter was generated by PCR using promoter-specific primers (Suppl. Table S2). Forward and reverse primers were designed with EcoRI sites and all amplification products were digested with EcoRI and cloned directly into EcoRI-digested LJP138, which contains an EcoRI site directly upstream of the GUS reporter gene (Pillitteri et al. 2007).
For promoter complementation using the 175-bp fragment, primers were designed to amplify −500 to −325 bp of the MUTE promoter. The amplicon was blunt-cloned into LJP312 containing the 35S CaMV minimal promoter element from −46 to +8 bp (Bhullar et al. 2007). For the second PCR reaction, primers were designed to with EcoRI sites. The amplicon included the −500 to −325-bp fragment fused to the 35S CaMV minimal promoter. This amplicon was digested with EcoRI and cloned directly into EcoRI-digested LJP138. For the 86 bp fragment, complementary oligos were designed that contained the MUTE promoter sequence from −411 to −325-bp upstream of the 35S CaMV minimal promoter The oligos were designed to produce EcoRI overhangs when hybridized (Suppl. Table S3). Oligos were resuspended at the same molar concentration in annealing buffer (10 mM Tris pH 8.0, 50 mM NaCl, 1 mM EDTA). For annealing, oligos were combined in equimolar amounts, heated to 95 °C for 5 min followed by slow cooling (1 °C/min) to reach room temperature. Annealed oligos were used directly in a ligation reaction with EcoRI-digested LJP138.
Site-directed mutagenesis
Two independent PCR reactions were performed for each mutagenesis reaction (Heckman and Pease 2007) using primers in Suppl. Table S4. Final amplification products containing the mutagenized cis-element were gel-purified using an UltraClean PCR Clean-Up Kit (MO BIO Laboratories, Inc.) and cloned into TOPO pENTR (Invitrogen) according to manufacturer’s instructions. To produce GFP reporter constructs, vectors were combined with pGWB4 destination vector (Nakagawa et al. 2007) containing the recombination sites upstream of the coding sequence of the GFP using LR Clonase (Invitrogen).
Histochemical staining and microscopy
Histochemical analysis of GUS activity was performed in GUS reaction buffer (0.5 mg/ml 5-bromo-4-chloro-3-indolyl-beta-d-glucuronide in 100 mM sodium phosphate, pH 7.0) as described previously (Pillitteri et al. 2008). GUS-stained samples were taken through an ethanol series to remove chlorophyll and placed in chloral hydrate solution (1:8:1, by vol., glycerol:chloral hydrate:water) for 1 h before imaging. All samples were viewed under DIC optics using a Leica Leitz DMRB microscope (Buffalo Grove, IL, USA) equipped with SPOT RT3 Cooled CCD camera (Sterling Heights, MI, USA). Confocal images were taken on an Olympus FV1000 (Center Valley, PA, USA). Cell borders were visualized with propidium iodide. Confocal images were false colored and brightness/contrast setters were adjusted using Photoshop CS4.
Analysis of promoter sequences
Regulatory elements in the MUTE promoter were analyzed using the online program PLACE (a database of plant cis-acting regulatory DNA elements) and PlantCARE (plant cis-acting regulatory elements, enhancers and repressors). These two programs are available at http://www.dna.affrc.go.jp/PLACE/ and http://bioinformatics.psb.ugent.be/webtools/plantcare/html/, respectively. Alignment of orthologous promoters was performed using Clustal Omega with manual adjustments.
Results
Promoter and transcript analysis of MUTE
Untranslated regions (UTRs) of mRNA transcripts are important contributors to transcript stability, spatial and temporal expression patterns, and translation efficiency (Molina and Grotewold 2005; Srivastava et al. 2014; Kim et al. 2014). Alternate transcriptional start sites (TSS) and polyadenylation can produce diversity that further affects mRNA transport or stability to impact gene expression (Shen et al. 2008). Given the transient and meristemoid-specific expression of MUTE, we were interested in accurately mapping the TSS and polyadenylation sites of the MUTE transcript. To this end, we performed 5′ and 3′ RACE (rapid amplification of cDNA ends). Our results indicate that MUTE has an invariable TSS (n = 9) 29 bp downstream of the TATA-box consensus sequence (TATAAAT). The 5′ UTR is 85 bp in length, which is consistent with the trend for short 5′ UTRs in TATA-box-containing promoters in Arabidopsis (Kawaguchi and Bailey-Serres 2005; Molina and Grotewold 2005) (Fig. 2).
In contrast to the uniform TSS, MUTE has multiple polyadenylation sites clustered in close proximity to one another (Fig. 2). The range of 3′ UTR size was between 70 and 142 bp, which is smaller than the average 3′ UTR in Arabidopsis (Shen et al. 2008). Variation in 3′ UTR length is common and several studies have determined that cell proliferation or developmental stage can cause a shift in polyadenylation sites (Proudfoot 2011). Messenger RNAs with longer 3′ UTRs have more potential for miRNA binding that can impact stability under specific developmental states (Sandberg et al. 2008; Proudfoot 2011). We found no evidence of miRNA binding sites within the 3′ UTR or anywhere in the MUTE coding region using publicly available data, suggesting that MUTE expression is not regulated by known miRNAs (Zhang et al. 2010).
Delineation of sequence elements required for stomatal-lineage-specific expression
The activity of the full-length MUTE promoter (1.9 kb from the translational start site) has been analyzed previously and shown to be specific to the stomatal lineage and hydathode. No expression outside the stomatal lineage has been observed using the full-length promoter (Pillitteri et al. 2007, 2008). To gain insight into the functional role of putative cis-elements in the MUTE promoter, progressive 5′ promoter deletions were generated through PCR amplification and used to drive expression of the β-glucuronidase (GUS) reporter gene (Fig. 3, Suppl. Fig. S1). At least six independent transgenic lines were selected for histochemical analysis for each construct (Fig. 4). We detected high and consistent levels of ectopic reporter expression outside the stomatal lineage using small promoter fragments and promoterless constructs (Fig. 4l, n, p). This ectopic expression made quantitative fluorometric or PCR analysis on the reporter lines irrelevant. Therefore, we relied on the detectable qualitative changes within the stomatal lineage to delimit the MUTE 5′-regulatory region (Figs. 3, 4).
Using the full-length promoter, MUTE expression initiates in a subset of meristemoids and is observed only in stomatal-lineage cells in the epidermis (Fig. 4a). Using qualitative assessment, no observable difference in spatial or temporal expression of MUTE promoter activity was identified for promoters at least 500 bp in length compared to full-length (Fig. 4a–f, Suppl. Fig. S1). However, promoter fragments that were less than 500 bp in length resulted in a qualitative reduction and ultimately absence of detectable GUS expression in the stomatal lineage (Fig. 4g–n). Specifically, a consistent reduction in GUS activity was observed using 469 and 443 bp of the MUTE promoter compared to full-length (Fig. 4g–j). Further reduction in promoter size dramatically reduced activity, where 411 bp displayed minimal activity and 325 bp generated no detectable expression in the stomatal lineage, identical to the promoterless control plants (Fig. 4m–p). Thus, we identified a graduated decrease in expression with increasing loss of promoter sequence. We conclude that the minimal elements necessary for stomatal-lineage specific expression reside between 500 and 325 bp upstream of the translational start site of MUTE.
175 bp-region is sufficient to initiate meristemoid-specific expression
Our deletion analysis revealed a short region from −500 to −325 bp that is important for MUTE promoter activity. To determine if this 175-bp region was sufficient to drive reporter gene expression, we joined one copy of the MUTE promoter fragment (−500 to −325 bp) to the 35S cauliflower mosaic virus (CaMV) minimal promoter (−46 to +8 bp relative to TTS) and analyzed its ability to drive GUS expression. This region of the CaMV promoter has been demonstrated to function as a core binding site (Oropeza-Aburto et al. 2012). The 175-bp chimeric promoter was sufficient to activate expression in the stomatal lineage, similar to the full-length promoter (Figs. 4a, 5), although expression intensity using the chimeric promoter was consistently weaker than full length. In addition to stomatal-lineage expression, we observed a low number of isolated patches of expression in vasculature tissue using the chimeric fragment that we do not observe using the native full-length promoter. This data indicates that key elements sufficient for proper spatiotemporal expression are present between −500 and −325 bp of the MUTE promoter.
To further dissect the functionality of this promoter fragment, we investigated a subregion of the 175-bp fragment (−411 to −325 bp) for its ability to initiate meristemoid-specific expression (Suppl. Fig. S2). This region was chosen based on our promoter deletion analysis, where the 411-bp promoter displayed very weak activity (Fig. 4k), suggesting that this region contained elements sufficient for basal expression. In contrast to the 175-bp fragment, none of the lines examined using the −411 to −325 bp fragment produced stomatal-lineage specific GUS expression (Suppl. Fig. S2). This smaller 86-bp region fused to the 35S CaMV minimal promoter was unable to mimic MUTE expression in the absence of the native 3′-flanking sequence. Taken together, these data suggests that the 89-bp region between −500 and −411 bp contains regulatory elements that are critical for robust MUTE expression. This is consistent with the notable loss of expression in our deletion experiments when the region from −500 to −411 bp is removed from the native promoter (Fig. 4g–l).
Known cis-elements are not required for MUTE expression
The promoter deletion and promoter sufficiency analysis suggested that elements between −500 and −325 bp were important for activity. Therefore, we identified potential cis-acting elements within this region of the MUTE promoter using publically available data (Table 1). In silico analysis of this promoter fragment showed that it contained 6 putative cis-elements including an E-box, [homeodomain-leucine zipper (HD-box), Dc3 promoter binding factor element (DPBF), GT2, L1-Box, and several Dof binding motifs (Table 1; Suppl. Fig. S2] (Dehesh et al. 1990; Abe et al. 2001; Kim et al. 2002; Davuluri et al. 2003; Bao et al. 2004; Yanagisawa 2004; Chang et al. 2008; Gordan et al. 2013). The DPBF and GT2 elements overlap (Fig. 6a).
To determine if these known cis-elements were specifically required for MUTE promoter activity, we performed site-directed mutagenesis to disrupt these elements in the context of a functional MUTE promoter (Suppl. Fig. S3). For this analysis we focused on elements in the region between −500 and −411 bp because they appear to be necessary for robust expression (Fig. 4e–j). Reporter constructs were produced by inserting the mutated promoter sequence upstream of either green fluorescent protein (GFP) or GUS. If any of these elements are important for MUTE expression, mutagenizing the sites should knock-down or eliminate reporter gene expression. Our data indicate that loss of these regulatory elements in the combinations used in this study did not decrease reporter gene expression (Fig. 6b–g). MUTE promoter activity with regard to intensity and spatiotemporal pattern was maintained across all constructs tested. This indicates that either a novel element not tested in this study is responsible for the stomatal-lineage specific expression of MUTE or that combinatorial control among multiple elements not tested in this study is required for proper expression.
Comparison of MUTE promoters in the Brassicaceae
To identify similarities in promoter structure among closely related members of the Brassicaceae, we aligned the fragment from A. thaliana MUTE promoter shown to be important for expression with those of orthologous promoters in A. lyrata, Brassica rapa and Capsella grandiflora (Fig. 7). The sequence used in our analysis was 92 % identical to A. lyrata, 84 % identical to B. rapa and 74 % identical to C. grandiflora, consistent with their phylogenic relationship. Of the specific known elements identified and tested in this study, only the Dof elements are spatially conserved among all promoter fragments, suggesting they may be important in regulation. The promoter region between −411 and −325 bp contains three conserved Dof elements, whereas the region between −469 and −411 bp contains one conserved Dof element (Fig. 7). In addition, an L1-box is conserved among three of the promoters. This element was not specifically tested here because a strong loss of promoter expression was detected even when the L1-box was intact (Fig. 4k). Although E-boxes were identified in the 1-kb sequence upstream of the start codon in all promoters, none of the related species had a conserved E-box element within the promoter region identified as important for MUTE expression in A. thaliana. In addition to the known elements, there are several conserved areas among all promoters that are not known binding sites for transcription factor or elements that affect transcription.
Discussion
The bHLH protein MUTE triggers the transition from meristemoid to GMC, making it a critical regulator of the cellular decision to divide or differentiate. Master regulators that control important cellular transitions have been identified in many developmental contexts (Weintraub et al. 1991; Ghysen et al. 1993) and genome-scale analyses have provided information about potential regulatory networks responsible for differentiation of cell types (Pillitteri et al. 2011; Tripathi et al. 2014; Adrian et al. 2015; Kuenne et al. 2015). However, identification of specific regulatory sequences is limited using these approaches. Using the MUTE promoter, we generated transgenic Arabidopsis plants carrying reporter constructs driven by serial promoter deletions to identify important 5′-regulatory regions. Through progressive removal of regulatory sequence we identified a region of the MUTE promoter that is both necessary and sufficient for proper expression.
Our results indicated that MUTE promoter function markedly decreased in the absence of an 89-bp fragment between nucleotides −500 and −411 bp (Fig. 4). Although the native −411-bp promoter was able to activate transcription at a low level (Fig. 4k), this region was insufficient to drive expression in the context of the CaMV chimeric promoter (Suppl. Fig. S2). This data, together with the fact that the 175-bp fragment from −500 to −325 bp was sufficient to activate transcription suggests that important elements are located in the region between −500 and −411 bp. The less robust transcription from our chimeric promoter fragments may reflect the need for specific basal transcription factors at the minimal promoter, which has been shown to be important in the specific expression of several genes (Rabenstein et al. 1999; Butler and Kadonaga 2002).
A recent study by Lau et al. (2014) determined that SPCH binds the MUTE promoter at −1482 and −220 bp from the translational start site. Their work suggested a possible direct link between the bHLH transcription factors that promote stomata development. The homeodomain-leucine zipper protein, HOMEODOMAIN GLABROUS2 (HDG2), has also been shown to bind the MUTE promoter (Peterson et al. 2013). Specifically, HDG2 transcriptionally activated MUTE in a transient assay by binding to the L1-box. The data presented here do not support that those regions of the promoter are critical for MUTE expression, but our data do not exclude the possibility of combinatorial control where alternate binding sites outside of our 175-bp region may function to enhance MUTE expression or change expression under variable conditions.
Gene-specific transcriptional regulation is mediated by cis-acting elements that specify the site, timing and level of gene expression. Specific known elements between −500 and −411 bp, including Dof elements, E-box, HD-box, and a GT2 element were independently mutagenized and did not result in a detectable change in promoter activity (Fig. 6, Suppl. Fig. S3). This suggests that the regulatory elements that drive MUTE expression may work in combination and therefore loss of any single element does not disrupt expression to a detectable extent. Alternatively, an individual element not tested in study is responsible for MUTE expression. Although there are many documented cases of individual elements being responsible for the majority of gene expression (Ulmasov et al. 1997; Gomez-Porras et al. 2007; Oropeza-Aburto et al. 2012), the graduated reduction of expression in the MUTE promoter suggests combinatorial control may be important. Combinatorial regulation has been demonstrated for many genes and it may be particularly important for genes responding to multiple pathways. It has been well established that stomatal development is an adaptable trait that is controlled by many different inputs such as light, CO2, humidity, temperature, and constitutive developmental pathways (Gray et al. 2000; Lake and Woodward 2008; Kang et al. 2009; Casson and Hetherington 2010; Pillitteri and Torii 2012). Due to the critical importance of stomata and their plastic development, it is perhaps not surprising that a single element was not identified in this study.
Comparison of the Arabidopsis promoter sequence with those of orthologs revealed conserved regions, some of which did not include known cis-elements. Among known elements, only the clustered placement of Dof core binding motifs (AAAG) was conserved among all four orthologous promoters. Clusters of AAAG sites have been shown to additively contribute to guard cell-specificity of AtMYB60 (Plesch et al. 2001; Cominelli et al. 2011). Our delineated area between −500 and −325 bp contains six Dof binding sites, higher than what would occur randomly in a sequence that size. Four of the Dof elements are conserved among close relatives. Our promoter truncation series removes sequential conserved Dof elements, therefore, our promoter comparison in combination with promoter deletion analysis suggests that Dof transcription factors may be important for MUTE regulation. We identified additional conserved sequences in the promoter that are not known to bind transcription factors, however, the absolute conservation among the orthologs may suggest some functional importance (Fig. 7). Overall, it is likely that MUTE is under the control of many loci, which activate or inhibit expression to fine-tune the production of stomata. With the identification of a promoter region important for MUTE expression, we have tools to investigate proteins bind and regulate MUTE, which ultimately contributes to mapping the genetic network controlling stomata production.
Author contribution statement
LJP conceived and designed research. AM, EMA, RAB, AFW, JJF, KCS and LJP conducted experiments. LJP, AM and AW analyzed data. LJP wrote the manuscript. All authors read and approved the manuscript.
Abbreviations
- Dof:
-
DNA binding with one finger
- GUS:
-
β-Glucoronidase
- GMC:
-
Guard mother cell
- GFP:
-
Green fluorescent protein
- CaMV:
-
Cauliflower mosaic virus
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This work was supported by a Grant from the National Science Foundation (#1147132) to LJP.
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425_2015_2445_MOESM1_ESM.tif
Suppl. Fig. S1 Comparison of MUTE promoter activity. Confocal images of abaxial leaf epidermis. a MUTE full-length promoter driving green fluorescent protein (GFP). b 1305 bp of the MUTE promoter driving GFP. Promoter length is relative to the translational start site (+1). Cell borders are stained with propidium iodide. Scale bar 10 µm (TIFF 6131 kb)
425_2015_2445_MOESM2_ESM.tif
Suppl. Fig. S2 Regulatory region complementation. a Diagram of the complementation construct. The MUTE promoter fragment from −411 to −325 bp was fused to the 35S CaMV minimal promoter (−46 to +8 bp) (Oropeza-Aburto et al. 2012). b, c DIC image of the abaxial leaf epidermis from 12-day old seedlings. Full-length MUTE promoter driving GUS expression (b) and complementation construct driving GUS expression (c). Scale bar 10 µm for epidermal images (TIFF 8236 kb)
425_2015_2445_MOESM3_ESM.tif
Suppl. Fig. S3 Site-directed mutagenesis. a Sequence of the MUTE promoter fragment; location of specific elements are indicated. Nucleotide position is relative to translational start site (+1). b–d Changes made to designated elements are indicated. All nucleotide substitutions were made in the context of a functional >522-bp promoter (TIFF 12019 kb)
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Mahoney, A.K., Anderson, E.M., Bakker, R.A. et al. Functional analysis of the Arabidopsis thaliana MUTE promoter reveals a regulatory region sufficient for stomatal-lineage expression. Planta 243, 987–998 (2016). https://doi.org/10.1007/s00425-015-2445-7
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DOI: https://doi.org/10.1007/s00425-015-2445-7