Jassim M Al-Hassan
Kuwait University, Biological Sciences, Faculty Member
Research Interests:
Research Interests:
... K~ot,oif Received Decembel-5. 1977' M~HAMED S. EL-EAZBY, JASSI~I M. AL-HASSAX, NAMEK F. EWEISS, and FARIDA AL-MASSAAD. Can. J. Chem. 57. ... M ~ HAMED S. EL-EAZBY, JASSIM M. AL-HASSAN, NAMEK F. EWEISS et FARIDA... more
... K~ot,oif Received Decembel-5. 1977' M~HAMED S. EL-EAZBY, JASSI~I M. AL-HASSAX, NAMEK F. EWEISS, and FARIDA AL-MASSAAD. Can. J. Chem. 57. ... M ~ HAMED S. EL-EAZBY, JASSIM M. AL-HASSAN, NAMEK F. EWEISS et FARIDA AL-MASSAAD. Can. J. Chem. 57. ...
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The Arabian catfish produces venoms from the skin (SV) and glands near the pectoral spines (GV). 1. The venoms have a high protein concentration, only limited amounts of lipid, carbohydrate and nucleic acid. Enzyme activities included... more
The Arabian catfish produces venoms from the skin (SV) and glands near the pectoral spines (GV). 1. The venoms have a high protein concentration, only limited amounts of lipid, carbohydrate and nucleic acid. Enzyme activities included alkaline phosphatase and esterases. Both SV and GV induce hemolysis. 2. SV and GV induced a dose-dependent smooth muscle contraction of human umbilical artery preparations. This response was competitively inhibited by atropine, suggesting a cholinergic response. 3. Heat treatment of GV did not decrease activity, while heating SV reduced it. 4. In-vivo experiments showed mainly a depressor response in rat and guinea-pig.
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A galactose specific lectin was isolated from an epidermal proteinaceous gel secretion of the Arabian Gulf catfish, Arius thalassinus, Ruppell. The lectin was extracted and purified to near homogeneity by exclusion chromatography,... more
A galactose specific lectin was isolated from an epidermal proteinaceous gel secretion of the Arabian Gulf catfish, Arius thalassinus, Ruppell. The lectin was extracted and purified to near homogeneity by exclusion chromatography, affinity chromatography and isoelectric focusing. The lectin appears to be active as a single polypeptide chain with a mol. wt near 200,000, which can form oligomers and heteropolymers. The lectin comprises about 2% of the total gel protein, lacks carbohydrates and contains no unusual types or amounts of amino acids. The lectin agglutinates a wide range of red blood cell types.
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1. When threatened or injured, the Arabian Gulf catfish (Arius thalassinus, Ruppell) secretes a thick gel-like layer of proteinaceous material to its skin surface mainly from unicellular glands of the epidermis termed club cells. 2. Since... more
1. When threatened or injured, the Arabian Gulf catfish (Arius thalassinus, Ruppell) secretes a thick gel-like layer of proteinaceous material to its skin surface mainly from unicellular glands of the epidermis termed club cells. 2. Since a preparation from this secretion has been implicated in stimulation of the rate of wound healing in man and other test animals, the total gel protein composition was analysed by chemical, chromatographic and electrophoretic techniques. 3. Gel proteins were separated into soluble and insoluble fractions by extractions with increasingly strong solubilizing agents and the most insoluble components were solubilized only upon treatment with 10% SDS or concentrated organic acids. 4. Some of the soluble proteins from the secretion are also present in the insoluble protein fractions, indicating that the insoluble material is formed in part by aggregation of the soluble proteins. 5. The secretion was shown to be distinct from the catfish venom and differed...
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High levels of platelet activating factor (PAF) activity were demonstrated by platelet aggregation and serotonin release assays to be present in fright induced epidermal secretions of the Arabian Gulf catfish, Arius bilineatus... more
High levels of platelet activating factor (PAF) activity were demonstrated by platelet aggregation and serotonin release assays to be present in fright induced epidermal secretions of the Arabian Gulf catfish, Arius bilineatus (Valenciennes, 1840). The PAF activity was purified by thin-layer chromatography. Mass spectral analysis combined with chemical and enzymatic modification of the purified PAF and inhibitor studies indicated that PAF activity was due to the presence of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC) molecules. The total AGEPC concentration in the epidermal secretions based on PAF assays was 8 x 10(8) M, well above the threshold level for platelet activation which is near 5 x 10(-11) M. Thus, stimulated epidermal secretory cells of Arius bilineatus supply platelet activating molecules at physiologically high concentrations to sites of injury.
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Research Interests:
1. The Arabian Gulf catfish produces proteinaceous epidermal secretions when threatened or injured. 2. The soluble fraction of the catfish epidermal secretions (SES) has vasoconstrictor activities on sheep renal arteries, which can be... more
1. The Arabian Gulf catfish produces proteinaceous epidermal secretions when threatened or injured. 2. The soluble fraction of the catfish epidermal secretions (SES) has vasoconstrictor activities on sheep renal arteries, which can be inhibited by several antagonists, including atropine, indomethacin, prazosin, and verapamil. 3. Mepyramine, yohimbine, and ketanserin have negligible effects on SES-induced contraction. 4. SES exhibits a significant tachyphylaxis upon addition of a second (8.4% reduction) and third (47% reduction) dose of SES to the organ bath, which can be partially prevented by addition of a fresh arterial section prior to each addition. 5. A vasoconstricting activity has been partially purified from SES by gel filtration on Fractogel HW-65(F) and appears to be a protein with a pI near 7.3. This activity is affected only by verapamil and prazosin.
Research Interests: Indian Ocean, Sheep, Animals, Male, Proteins, and 3 moreEpidermis, Muscle contraction, and Renal Artery
1. A fright or shock induced toxic secretion (gel) from the epidermis of the Arabian Gulf catfish, Arius thalassinus, exhibits hemolytic activity when tested against red blood cells from many different sources. 2. An enzyme with hemolytic... more
1. A fright or shock induced toxic secretion (gel) from the epidermis of the Arabian Gulf catfish, Arius thalassinus, exhibits hemolytic activity when tested against red blood cells from many different sources. 2. An enzyme with hemolytic activity, which represents 1.1% of the total soluble gel protein fraction, has been purified to homogeneity. 3. Molecular sieve chromatography and SDS polyacrylamide gel electrophoresis of the purified protein indicate a mol. wt of 34,000. 4. One additional protein component with hemolytic activity was found in the epidermal secretion. 5. Specific activity of the catfish epidermal factor is 20.6 units/mg protein, a level somewhat lower than those of most protein hemolytic factors. 6. The catfish hemolytic factor was not ichthyotoxic when tested against small fish and did not cause lethality when administered intravenously to rabbits.
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1. Tohoku J Exp Med. 1987 Sep;153(1):1-4. A simple spectrophotometric method for the determination of thrombin activity. Hussein KA, Al-Hassan JM. Biochemistry Department, Faculty of Science, Kuwait University. The activity ...
A simple spectrophotometric method for the quantitating of the clot promoting activity of thromboplastin in commercial preparations was developed. Thromboplastin activities were proportional to the maximum rate of clot formation. This was... more
A simple spectrophotometric method for the quantitating of the clot promoting activity of thromboplastin in commercial preparations was developed. Thromboplastin activities were proportional to the maximum rate of clot formation. This was calculated from curves of absorption change during the coagulation of plasma coagulation control. The clot-promoting effects of thromboplastin in commercial preparations varied widely when assayed by this method.
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... View Record in Scopus | Cited By in Scopus (0). HA Krebs and LV Eggleston, The role of pyruvate kinase in the regulation of gluconeogeresis, Biochem. ... M Brin, Frythrocyte as a biopsy tissue for functional evaluation of thiamine... more
... View Record in Scopus | Cited By in Scopus (0). HA Krebs and LV Eggleston, The role of pyruvate kinase in the regulation of gluconeogeresis, Biochem. ... M Brin, Frythrocyte as a biopsy tissue for functional evaluation of thiamine adequacy, JANA 187 (1964), pp. 762766. ...
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Research Interests:
1. Lancet. 1983 May 7;1(8332):1043-4. Accelerated wound healing by a preparation from skin of the Arabian Gulf catfish. Al-Hassan JM, Thomson M, Criddle RS. PMID: 6133079 [PubMed - indexed for MEDLINE]. Publication Types: Letter; Research... more
1. Lancet. 1983 May 7;1(8332):1043-4. Accelerated wound healing by a preparation from skin of the Arabian Gulf catfish. Al-Hassan JM, Thomson M, Criddle RS. PMID: 6133079 [PubMed - indexed for MEDLINE]. Publication Types: Letter; Research Support, Non-US Gov't. ...
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... Page 14. 486 JM A1-Hassan et al. CHANDY, M., 1954. ... DAY, F., 1877. The Fishes of India: being a natural history of the fishes known to inhabit the seas and fresh waters of India, Burma and Ceylon. London: W. Dawson and Son, 2 vols,... more
... Page 14. 486 JM A1-Hassan et al. CHANDY, M., 1954. ... DAY, F., 1877. The Fishes of India: being a natural history of the fishes known to inhabit the seas and fresh waters of India, Burma and Ceylon. London: W. Dawson and Son, 2 vols, XX+ 778pp., 198pls. ...
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... Titre du document / Document title. Isolation of 1,2-dihydroxy-4-glucosyloxynaphthalene from Lawsonia innermis. Auteur(s) / Author(s). MUHAMMAD AFZAL (1) ; GALIB AL-ORIQUAT ; AL-HASSAN JM ; NAZAR MUHAMMAD ; Affiliation(s) du ou des... more
... Titre du document / Document title. Isolation of 1,2-dihydroxy-4-glucosyloxynaphthalene from Lawsonia innermis. Auteur(s) / Author(s). MUHAMMAD AFZAL (1) ; GALIB AL-ORIQUAT ; AL-HASSAN JM ; NAZAR MUHAMMAD ; Affiliation(s) du ou des auteurs / Author(s) Affiliation( ...
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Research Interests:
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High levels of platelet activating factor (PAF) activity were demonstrated by platelet aggregation and serotonin release assays to be present in fright induced epidermal secretions of the Arabian Gulf catfish, Arius bilineatus... more
High levels of platelet activating factor (PAF) activity were demonstrated by platelet aggregation and serotonin release assays to be present in fright induced epidermal secretions of the Arabian Gulf catfish, Arius bilineatus (Valenciennes, 1840). The PAF activity was purified by thin-layer chromatography. Mass spectral analysis combined with chemical and enzymatic modification of the purified PAF and inhibitor studies indicated that PAF activity was due to the presence of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC) molecules. The total AGEPC concentration in the epidermal secretions based on PAF assays was 8 x 10(8) M, well above the threshold level for platelet activation which is near 5 x 10(-11) M. Thus, stimulated epidermal secretory cells of Arius bilineatus supply platelet activating molecules at physiologically high concentrations to sites of injury.