Sabina Shrestha
Address: Biotechnology Laboratory (Lab. No. 5151)
College of Applied Life Sciences
Jeju National University
66 Jejudaehakno
Jeju 690-756
South Korea
College of Applied Life Sciences
Jeju National University
66 Jejudaehakno
Jeju 690-756
South Korea
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properties and total phenol content. The modifi ed Folin-Ciocalteu method was used to determine total phenol content and 2,2-diphenyl-1-picrylhydrazyl
radicals (DPPH•) assay for antiradical activity. In all samples, physicochemical parameters like moisture, reducing sugar, sucrose, ash, free
acidity and water insoluble matter were also measured according to harmonized methods of the International Honey Commission (IHC). The results
of physicochemical analysis showed that all the values, except for moisture of a small number of high altitude honey samples, are in good agreement
with the current Nepalese standard. The total phenolic contents of honey, collected from high and low altitude, ranged from 154.87 to 41.90 mg gallic
acid equivalent (GAE/100 g) respectively, at corresponding antiradical activity using DPPH• expressed as percent inhibition of 76.66% and 25.69%.
The IC50 values of selected high altitude honey samples ranged from 56 to 72 mg/mL. The total antioxidant properties were correlated (P<0.01) between
total phenol content and antiradical activity (r=0.992). The obtained results demonstrate that the Nepalese honey collected from high altitude
region contained more antioxidants than honey of low altitude region.
properties and total phenol content. The modifi ed Folin-Ciocalteu method was used to determine total phenol content and 2,2-diphenyl-1-picrylhydrazyl
radicals (DPPH•) assay for antiradical activity. In all samples, physicochemical parameters like moisture, reducing sugar, sucrose, ash, free
acidity and water insoluble matter were also measured according to harmonized methods of the International Honey Commission (IHC). The results
of physicochemical analysis showed that all the values, except for moisture of a small number of high altitude honey samples, are in good agreement
with the current Nepalese standard. The total phenolic contents of honey, collected from high and low altitude, ranged from 154.87 to 41.90 mg gallic
acid equivalent (GAE/100 g) respectively, at corresponding antiradical activity using DPPH• expressed as percent inhibition of 76.66% and 25.69%.
The IC50 values of selected high altitude honey samples ranged from 56 to 72 mg/mL. The total antioxidant properties were correlated (P<0.01) between
total phenol content and antiradical activity (r=0.992). The obtained results demonstrate that the Nepalese honey collected from high altitude
region contained more antioxidants than honey of low altitude region.