Research Interests:
Research Interests:
Research Interests:
Research Interests:
Natural plant products have been used in Nigerian folk medicine in the management of sickle cell anemia by inhibiting sickling. This work was therefore aimed at investigating the Antisickling potential of the ethanol seed extract of Vigna... more
Natural plant products have been used in Nigerian folk medicine in the management of sickle cell anemia by inhibiting sickling. This work was therefore aimed at investigating the Antisickling potential of the ethanol seed extract of Vigna unguiculata (E1) and Vigna subterranean (E2) used in the Nigerian herbal medicine with a view of proposing an effective herbal recipe for the management of sickle cell disease. Preliminary phytochemistry, sickling inhibition test, sickling reversal test and polymerization test were carried out using standard methods. The phytochemical analysis indicated the presence of saponins, reducing sugar, carbohydrate, fats and oil, steroids, glycosides, alkaloids and proteins in E1 and the presence of flavonoids, saponins, carbohydrates, fats and oil, resins, terpenoids, steroids, glycosides, alkaloids and proteins in E2. The results of the antisickling test showed that E1 and E2 had significantly (p<0.05) higher antisickling effect than the Hbss control with E1 showing a higher percentage than E2. The percentage sickling reversal effect of E1 was slightly lower than E2 but their reversal of sickling were significantly higher than the control. The result of the polymerization showed that both extracts significantly (p<0.05) increased delayed time before polymerization at 50, 25 and 12% concentrations compared to the control. From the results, the extracts Vigna Unguiculata and Vigna Subterranean have shown to be therapeutically beneficial in the management of sickle cell disease and thus are strongly recommended by this study to be developed into supplements for the management of sickle cell disease.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests: Traditional Medicine, Biology, Pepper, Histology, Toxicity, and 3 morePiper, Acute Toxicity, and Alloxan
Research Interests:
Research Interests:
Research Interests:
Aims: To determine the effect of cooking on proximate, phytochemical constituents and their changes in hematological parameters. Study Design:Determination of proximate and quantitative phytochemical constituents of the cooked and... more
Aims: To determine the effect of cooking on proximate, phytochemical constituents and their changes in hematological parameters. Study Design:Determination of proximate and quantitative phytochemical constituents of the cooked and rawT.conophorum (CTC and RTC respectively) nut and the effect of the nut on the hematological indices on male albino rats fed with the cooked and raw diet formulations ofthe nut for 30 days period. Methodology: Rats were divided into six groups of five rats each. Each feed and walnut was weighed and mixed in the ratio of 1:1 before administration. Group A: Normal animal feed, Group B: Mixture of animal feed and cooked nut
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
This work evaluated the effects of different doses of the human urine on normal and alloxaninduced diabetic rats. The experimental design comprises of five (5) groups of four (4) animals each. At the end of the treatment period, blood... more
This work evaluated the effects of different doses of the human urine on normal and alloxaninduced diabetic rats. The experimental design comprises of five (5) groups of four (4) animals each. At the end of the treatment period, blood glucose levels, protein, MDA, catalase, Glutathione and vitamin C concentrations were determined in both the normal and diabetic rats. Oral administration of low and higher doses (5ml/kg and 10 ml/kg) of human urine for 7 days resulted in significant (p < 0.05) decrease in blood glucose and protein concentrations of the diabetic rats compared to the untreated diabetic rats. However, the catalase concentration of the rats treated with (5ml/kg and 10 ml/kg) of human urine showed a significant (p < 0.05) increase compared to the untreated diabetic rats. This results show that human urine has great potentials of increasing the ascorbic acid concentration of diabetic rats treated with standard drug, 5ml/kg and 10ml/kg of urine when compared to diabeti...
Research Interests:
Research Interests:
Research Interests:
This study was aimed at evaluate the effect of methanol leaf and fruit extracts of Kigelia africana on normal rats. The catalase, vitamin C, protein level, total cholesterol, High Density Lipoprotein, Low Density Lipoprotein, Alanine... more
This study was aimed at evaluate the effect of methanol leaf and fruit extracts of Kigelia africana on normal rats. The catalase, vitamin C, protein level, total cholesterol, High Density Lipoprotein, Low Density Lipoprotein, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and Malondialdehyde (MDA) concentration were determined. The experimental design comprises of twenty rats divided into four groups of five rats each. Group I served as a control while group II received 2.5mg/kg of glibenclamide, group III received 500mg/kg methanol leaf extract of Kigelia africana and group IV received 100mg/kg methanol fruit extract of Kigelia africana. The result showed a significant (p<0.05) difference in protein level and vitamin C concentration in groups III and IV when compared with group I and group II. For total cholesterol, ALT and MDA concentration, a significant (p<0.05) decrease in the treated groups (II, III and IV) was observed when compared to the normal (grou...
Research Interests:
The need to establish a broad base environmental monitoring and toxicological assessment of contaminants on water resources and public health gave rise to this investigation. Environmental contaminants monitoring is one of the key issues... more
The need to establish a broad base environmental monitoring and toxicological assessment of contaminants on water resources and public health gave rise to this investigation. Environmental contaminants monitoring is one of the key issues in understanding and managing hazards to human health and ecosystems. In many ways our modern day 'pollution wonder-environment' has turned into a contamination nightmare, and few examples are more startling than those caused by pathogens and other associated microorganisms. In this study, River water resources of Qua-Iboe River in Eket Community of Akwa Ibom State, was analysed. The analysed include biochemical, physicochemical and bacteriological quality. The results revealed that pH ranged from 4.66 to 7.51 with mean temperature 18 to 23 °C. Other physicochemical parameters including, biochemical oxygen demand, microbial characterization values exceeded the recommended level for surface water quality. Results of Bacteriological analyses s...
Research Interests:
The antioxidant and antihyperglycamic effects of herbal cocktail namely: Vernonia amygdalina, Gongronema latifolium, Allium sativium, Ocimum gratissmun and Aloe barbadensis were studied on alloxan-induced diabetic rat. Twenty (20) albino... more
The antioxidant and antihyperglycamic effects of herbal cocktail namely: Vernonia amygdalina, Gongronema latifolium, Allium sativium, Ocimum gratissmun and Aloe barbadensis were studied on alloxan-induced diabetic rat. Twenty (20) albino Wistar rats were equally divided into five groups. Groups 1 and 2 served as normal and untreated Diabetic controls. Test groups 3, 4 and 5 were treated with 10mg/kg glibenclamide, 200mg and 400mg/kg of extract for 28 days. The result showed a dose dependant significant increase (P<0.05) in serum concentrations of vitamin C, glutathione and catalase activity in test groups when compared to untreated diabetic control. Similarly, the extract significantly reduced (P<0.05) glucose concentration of diabetic rats at both concentrations when compared to untreated diabetic Rats. The results confirmed the already established use of these plants in traditional medicine and equally established their useful synergistic effect in reducing blood sugar and i...
Research Interests:
Traditional medicine still remains the main recourse for a large majority of people for treating health problems in African. Therefore, the aim of this work is to assess the toxicological effect of the fresh ripe fruits using two solvents... more
Traditional medicine still remains the main recourse for a large majority of people for treating health problems in African. Therefore, the aim of this work is to assess the toxicological effect of the fresh ripe fruits using two solvents for extraction. The toxicological evaluation of aqueous-methanol extract of Dennettia tripetala fresh ripe fruits at 100, 200 and 400 mg/kg body weight for 14 days on some biochemical parameters in wistar rats was investigated. The extract at all the doses tested show non-significant (p > 0.05) increase from the control in ALT, AST, ALP, total protein, albumin, direct bilirubin, creatinine, Na+ and K+, while the level of total bilirubin and urea show significant (p < 0.05) increase from the control at 400mg/kg body weight. The levels of SOD, GPx, GST, and GSH in the serum were significantly (p < 0.05) decrease in the treated rats at 200 and 400mg/kg body weight, whereas the level of MDA and CAT showed non-significant (p > 0.05) increase...
Research Interests: Chemistry, Horticulture, Pepper, Toxicity, Albumin, and 7 moreScientific, Antioxidant, Glutathione, Urea, Bilirubin, Acute Toxicity, and Creatinine
Research Interests:
Department of Biochemistry, University of Nigeria, Nsukka, Nigeria Author's Correspondence: oguguavictor@yahoo.com; parkeselisco@yahoo.co.uk +2348067346144; +2348034167192 and +2348037804687 ... ABSTRACT: Screening for the most... more
Department of Biochemistry, University of Nigeria, Nsukka, Nigeria Author's Correspondence: oguguavictor@yahoo.com; parkeselisco@yahoo.co.uk +2348067346144; +2348034167192 and +2348037804687 ... ABSTRACT: Screening for the most effective ...
Research Interests:
Research Interests: Chemistry, Pepper, Glibenclamide, Diabetes mellitus, Ethanol, and 4 moreBody Weight, Sorbitol, Piper, and Alloxan
Research Interests:
The effect of Colatropis gigantea leaf extract was studied on alloxan-induced hyperglycaemic rabbits to evaluate the possible hypoglycaemic and antioxidant properties of Colatropis gigantea (C. gigantean) in diabetes mellitus. Significant... more
The effect of Colatropis gigantea leaf extract was studied on alloxan-induced hyperglycaemic rabbits to evaluate the possible hypoglycaemic and antioxidant properties of Colatropis gigantea (C. gigantean) in diabetes mellitus. Significant decrease (p < 0.05) in the thiobarbituric acid reactive substance (TBARS) was observed in diabetic rabbits administered with the acetone fraction of Colatropis gigantea (C. gigantean) leaf when compared to diabetic control group. Consistent significant increases (p < 0.05) were noticed in the activities of erythrocyte antioxidant enzymes; catalase, superoxide dismutase, and glutathione peroxidase in diabetic rabbits treated with acetone fraction of C. gigantea leaf compared with diabetic control group, although higher activities of these enzymes were observed in diabetic rabbits treated with glibenclamide (reference drug) when compared to the group administered C. gigantea extract. It was also observed that C. gigantea extract had significant...
Research Interests:
Research Interests:
Toxicities of various kinds due to inhalation of fumes from paint industries as well as housing estates in Nigeria and indeed in many Africa countries are now being realized as an occupational hazard. In this study, the effects of paint... more
Toxicities of various kinds due to inhalation of fumes from paint industries as well as housing estates in Nigeria and indeed in many Africa countries are now being realized as an occupational hazard. In this study, the effects of paint fumes on oxidative stress and hematological parameters in diabetic and normal rats were investigated. The rats were exposed to paint fumes for 7 days. The result of groups 4-6 (diabetic) showed significant increase (P 0.05) in their glucose when compared to group 1 while group 5-6 gave a significant elevation of blood glucose level when compared to group 4. Red blood cell in groups 4-6 (diabetic groups) were significantly (P 0.05) in antioxidant levels across groups 1-6. The results of packed cell volume in group 6 (diabetic exposed to oil paint fumes) were significantly (P < 0.05) lower compared to group 1. Methemoglobin concentration showed a significant (P < 0.05) increase in groups 3 (normal rats exposed to oil paint fumes) and 6 (diabetic rats exposed to oil paint fumes) when compared to group 1. In conclusion, paint suppresses certain hematopoietic processes and complicates diabetic status in rats.
Research Interests:
Research Interests:
Research Interests:
Poisonings from household chemicals such as cleaning products and cosmetics are common occurrences and can cause acute respiratory infections and cancer. This study was therefore aimed at evaluating the level of prevention and management... more
Poisonings from household chemicals such as cleaning products and cosmetics are common occurrences and can cause acute respiratory infections and cancer. This study was therefore aimed at evaluating the level of prevention and management of accidental poisoning from household chemicals in the Nkwen Area in Bamenda, Cameroon. A cross-sectional population based descriptive study was undertaken in miles three and four Nkwen, Bamenda in which 110 households were randomly selected to take part. The evaluation was carried out by the use of a questionnaire survey. From the results obtained, detergents and cosmetics were the most available chemicals used in these areas, with cosmetics contributing to 32.22% of poisoned cases while detergents contributed to 51.11% of the poisoned cases. It was also found that most of the poisoned cases were not taken to the hospital. In conclusion, this study had shown that the level of prevention and management of accidental poisoning from household chemicals in the study areas were generally poor.
Research Interests:
The aim of this study was to assess the practice of radiation protection and compare it with the stated norms of radiation protection. A cross – sectional hospital based descriptive design was undertaken in four hospitals in Yaounde,... more
The aim of this study was to assess the practice of radiation protection and compare it with the stated norms of radiation protection. A cross – sectional hospital based descriptive design was undertaken in four hospitals in Yaounde, Cameroon in which all the technologists (17) working in the x – ray department of the selected hospitals took part in the study. The assessment was carried out by the use of a questionnaire survey. From the results obtained, all the hospitals had lead aprons and shields but only one hospital gonad shield and lead gloves. 47.06% had been trained for at least three years. Radiation protection supervisors were present in two of the four hospitals but only one hospital had a warning red light in good working condition. In conclusion radiation protection in conventional radiography is not properly practiced as not all norms are respected and therefore remains a problem that needs to be solved.
Research Interests:
Research Interests:
A comparative study on the effect of ethanol bark and methanol leaf extract of Kigelia africana on some biochemical parameters in alloxan induced diabetic rats, was carried out. The ethanol bark and methanol leaf extracts of this plant... more
A comparative study on the effect of ethanol bark and methanol leaf extract of Kigelia africana on some biochemical parameters in alloxan induced diabetic rats, was carried out. The ethanol bark and methanol leaf extracts of this plant was fed to alloxan-induced diabetic rats for a period of 14days and its effect on some biochemical parameters on the blood serum of the rats were assayed. Twenty-five (25) male albino rats were divided into five groups: normal rats (group 1), diabetic untreated rats (group 2), diabetic rats treated with glibenclamide (group 3), diabetic rats treated with ethanol bark extract (group 4) and diabetic rats treated with methanol leaf extract (group 5), with groups 1 and 2 serving as positive and negative control respectively. The treatment groups were orally administered 300mg/kg of each extract and their serum analysed for fasting blood sugar (FBS), MDA, Alanine aminotransferase (ALT) and Creatinine levels. The result reviewed significant (p<0.5) decre...
Research Interests:
Background Different parts of the Parkia biglobosa plant are employed in traditional medicine in different African communities. However, information ratifying its use and biochemical influence on health is still scanty in literature.... more
Background Different parts of the Parkia biglobosa plant are employed in traditional medicine in different African communities. However, information ratifying its use and biochemical influence on health is still scanty in literature. Thus, the present study assessed the influence of the ethyl acetate fraction of Parkia biglobosa leaves (EAFPB) on some biochemical parameters of sub-chronic fructose-loaded rats. Methodology Twenty-five Wistar rats were randomized into five groups ( n = 5). The normal control group was maintained on normal diet only while the high fructose solution (HFS) control (placebo), reference and treatment groups received high fructose solution (3 g/kg/d b.w of fructose) for 30 days before treatment. Based on pilot study, two doses (100 and 200 mg/kg/d b.w) of EAFPB were selected and were administered to two groups of test animals while the reference group received 300 mg/kg/d b.w. of metformin for 14 days. Thereafter, blood was collected from fasted animals fo...
Integrated environmental biotechnology-oriented framework for solid waste management and control in Nigeria
Research Interests:
The continuous fluctuation in power supply in some developing countries has been suggested to be one of the contributing factors that affect stored experimental blood samples leading to errors in laboratory results. The study was... more
The continuous fluctuation in power supply in some developing countries has been suggested to be one of the contributing factors that affect stored experimental blood samples leading to errors in laboratory results. The study was therefore aimed at evaluating the effect of vitamin-E stored blood samples on Malondialdehyde (MDA) and protein concentrations with a view to suggesting a way of reducing errors in laboratory results that are due to oxidation. MDA was determined using the method of Das et al. (1990) while Biuret's method as described in Amadi et al. (2004) was used to determine the protein concentration. The results obtained show that MDA concentration in the blood sample without vitamin E increased during storage from day 1 (80% TBARS) today 4 (233% TBARS) while in the sample stored with vitamin E, the concentration of MDA decreased slightly during storage from day2 (80% TBARS) today (79% TBARS). The protein concentration in the samples without vitamin E decreased duri...
Research Interests:
Since animals and animal products are very expensive sources of nutrients especially in developing countries, it is very important to exploit other sources of acquiring nutrients that will be less expensive. This work was therefore aimed... more
Since animals and animal products are very expensive sources of nutrients especially in developing countries, it is very important to exploit other sources of acquiring nutrients that will be less expensive. This work was therefore aimed at evaluating the nutritional and toxicological potentials of Mucuna pruriens seed in view of proposing a less expensive source of nutrient in developing countries. The phytochemical analysis was carried out using the method of Harbourne et al. (1973). The proximate analysis, elementary analysis and determination of toxicants were determined using standard biochemical methods. Results of the phytochemical analysis showed the presence of alkaloids, proteins, carbohydrates, saponins, tannins in high concentration; flavonoids in moderate concentration while cyanogenic glycosides and steroidal aglycones were absent. Proximate analysis revealed high percentage of crude protein (30.1±0.25%) while ash (8.2±0.2%), moisture (7.67±0.34%) and fiber (3.2±0.01%)...
Research Interests:
Oxidative stress has been linked to the pathogenicity of many diseases. This study investigated the total phenolic content (TPC) and total flavonoid content (TFC) of the methanolic extract and solvent fractions (n-hexane, ethyl acetate,... more
Oxidative stress has been linked to the pathogenicity of many diseases. This study investigated the total phenolic content (TPC) and total flavonoid content (TFC) of the methanolic extract and solvent fractions (n-hexane, ethyl acetate, n-butanol, and aqueous) of Desmodium ramosissimum using Folin–Ciocalteu and aluminum chloride assays, respectively. The extract and solvent fractions were further appraised for their in vitro antioxidant capacity using total antioxidant capacity (TAC), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and ferric reducing antioxidant power (FRAP) methods at varying concentrations of 25–300 µg/mL. Results revealed that ethyl acetate and n-butanol fractions possessed elevated levels of TPC and TFC when compared to other solvent fractions and extracts in a concentration-dependent manner. The ethyl acetate fraction had the highest TPC (532.36 mg GAE/g), TFC (2843.33 mg QE/g), and ferric reducing potential (56.70 mg GAE/g) at 300 µg/mL. In addition...
Research Interests:
Sphenostylis stenocarpa is a legume crop of Fabaceae family. Several studies have shown that the seeds are rich in chemical constituents capable of suppressing benign prostatic hyperplasia (BPH). The purpose of this study was to... more
Sphenostylis stenocarpa is a legume crop of Fabaceae family. Several studies have shown that the seeds are rich in chemical constituents capable of suppressing benign prostatic hyperplasia (BPH). The purpose of this study was to investigate effect of the seed extracts on experimentally induced BPH in rats. Thirty five male Wistar rats were randomized into seven groups of five rats each. BPH was induced in the rats by subcutaneous injection of testosterone propionate for 28 days and thereafter treated with ethanolic and petroleum ether extracts of the plant seeds at different doses (200 and 400 mg/kg body weight) for seven days. The effect of the extracts on BPH parameters and antioxidant indices were studied. Finasteride was used as a positive control. The results showed that treatment of the disease rats with 400 mg/kg body weight of the extracts resulted in a significant decrease (p S. stenocarpa seed could be useful in management of BPH.
Research Interests:
A methodology for producing biosurfactants from Submerged Fermented Fruit Bagasse of Yellow Cashew (Anacardium occidentale) Using Pseudomonas aeruginosa was determined. This involved controlling agitation, temperature, pH and salt... more
A methodology for producing biosurfactants from Submerged Fermented Fruit Bagasse of Yellow Cashew (Anacardium occidentale) Using Pseudomonas aeruginosa was determined. This involved controlling agitation, temperature, pH and salt concentration conditions. The emulsification capacity of the biosurfactant was also verified with engine oil, olive oil, kerosene, and petrol. Carbon sources were evaluated with regards to the growth and production. The stability of the rhamnolipid produced by Pseudomonas sp. was also evaluated for possible applications in Bioremediation, antimicrobial and emulsions. Its antimicrobial activity was checked against Staphylococcus aureus, Escherichia coli, Pseudomonas and Salmonella typhimurium. On the basis of high production of biosurfactant, it is suggested that the hydrolysate of yellow cashew bagasse is a good and cheapest source. The study concludes that Fruit Bagasse of Yellow Cashew waste are ideal substrate for biosurfactant production, which may hav...
The leaves of Mucuna pruriens have been used traditionally for the treatment of various ailments but little information has been documented about its toxicological studies of the aqueous leaf extract of mucuna pruriens on albino rats to... more
The leaves of Mucuna pruriens have been used traditionally for the treatment of various ailments but little information has been documented about its toxicological studies of the aqueous leaf extract of mucuna pruriens on albino rats to ascertain its hepatotoxic effect when used in traditionally medicine. Preliminary phytochemical analysis was carried out by the method of Harboune (1973). The ALT and AST concentrations were measured using Human test kits (Germany). Total protein and bilirubin were measured using standard biochemical methods as described in Amadi et al. 2004. The phytochemical analysis indicated the presence of flavonoids, tannins, terpenoids, alkaloids, saponins and trace amounts of cyanogenic glycosides. The leaf extract of graded doses of 400 and 800mg/kg body weight given orally to the rats caused no significant increase or decrease (p>0.05) in the serum ALT, AST, total protein and bilirubin concentrations when compared to the controls. From the results of our...
Oxidative stress is implicated in the pathogenesis of many chronic diseases. This study investigated the effect of methanol and chloroform extracts of root bark of Brenania brieyi on inflammation-induced oxidative stress in rats. Cotton... more
Oxidative stress is implicated in the pathogenesis of many chronic diseases. This study investigated the effect of methanol and chloroform extracts of root bark of Brenania brieyi on inflammation-induced oxidative stress in rats. Cotton pellet-induced inflammatory model was used to induce oxidative stress. The rats were treated with varying concentrations of each extract and indomethacin (standard drug) for 7 days. On day 8, their blood samples collected by cardiac puncture was used for the determination of biochemical parameters of oxidative stress such as the malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities along with reduced glutathione (GSH), vitamins C, and E. using standard methods. The bioactive compounds responsible for bioactivity were determined with gas chromatographymass spectrometry (GC-MS) techniques. Results obtained showed a significant (p < 0.05) decreases in malondialdehyde level, an increase in superoxid...
Research Interests:
Crude oil, refined petroleum products, as well as polycyclic aromatic hydrocarbons are ubiquitous in various environmental compartments. They can bioaccumulate in food chains where they disrupt biochemical or physiological activities of... more
Crude oil, refined petroleum products, as well as polycyclic aromatic hydrocarbons are ubiquitous in various environmental compartments. They can bioaccumulate in food chains where they disrupt biochemical or physiological activities of many organisms, thus causing carcinogenesis of some organs, mutagenesis in the genetic material, impairment in reproductive capacity and / or causing hemorrhage in exposed population. The cause / effect of oil pollutant are usually quantified by using biological end point parameters referred to as biomarkers. Contamination of soil arising from spills is one of the most limiting factors to soil fertility and hence crop productivity. These deleterious effects make it mandatory to have a counter measure for the petroleum hydrocarbon pollutant in the environment. Bioremediation of petroleum hydrocarbon-contaminated environment is a potentially important application of Environmental Biotechnology. In this approach microorganisms are utilized under some sp...