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Research Interests: Art and Actinobacteria
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Poster presentado en la S7. Biodeterioration: fundamentals, present and future perspectives. Session in honour of Prof. Cesareo Saiz Jimenez del 3rd International Congress Science and Technology for the Conservation of Cultural... more
Poster presentado en la S7. Biodeterioration: fundamentals, present and future perspectives. Session in honour of Prof. Cesareo Saiz Jimenez del 3rd International Congress Science and Technology for the Conservation of Cultural Heritage.-- Technoheritage 2017 21-24th May 2017, Cadiz, Spain
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The advent of molecular tools, and particularly next-generation sequencing, has dramatically changed our knowledge of the diversity of microbial life on Earth. In recent decades, many studies on different terrestrial environments have... more
The advent of molecular tools, and particularly next-generation sequencing, has dramatically changed our knowledge of the diversity of microbial life on Earth. In recent decades, many studies on different terrestrial environments have described the intriguing diversity and abundance of Euzebyales/Euzebyaceae/Euzebya, yet its role in the geochemical cycle of elements is unknown. In addition, as far as we know, no Euzebya isolates have been obtained from terrestrial niches. In this review, it is shown that Euzebya and other haloalkaliphilic bacteria can thrive under harsh conditions, such as high concentrations of sodium and/or calcium, high electric conductivity and alkaline pH, highly variable temperatures, and water fluctuations. These conditions are quasi-extreme in the studied terrestrial environments. However, the culture media used so far for isolation have failed to reproduce the original conditions of these terrestrial ecosystems, and this is likely the reason why strains of ...
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The genus Crossiella contains two species, C. equi, causing nocardioform placentitis in horses, and C. cryophila, an environmental bacterium. Apart from C. equi, which is not discussed here, environmental Crossiella is rarely reported in... more
The genus Crossiella contains two species, C. equi, causing nocardioform placentitis in horses, and C. cryophila, an environmental bacterium. Apart from C. equi, which is not discussed here, environmental Crossiella is rarely reported in the literature; thus, it has not been included among “rare actinobacteria”, whose isolation frequency is very low. After C. cryophila, only five reports cover the isolation of Crossiella strains. However, the frequency of published papers on environmental Crossiella has increased significantly in recent years due to the extensive use of next-generation sequencing (NGS) and a huge cascade of data that has improved our understanding of how bacteria occur in the environment. In the last five years, Crossiella has been found in different environments (caves, soils, plant rhizospheres, building stones, etc.). The high abundance of Crossiella in cave moonmilk indicates that this genus may have an active role in moonmilk formation, as evidenced by the prec...
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Research on cave microorganisms has mainly focused on the microbial communities thriving on speleothems, rocks and sediments; however, drip water bacteria and calcite precipitation has received less attention. In this study, microbial... more
Research on cave microorganisms has mainly focused on the microbial communities thriving on speleothems, rocks and sediments; however, drip water bacteria and calcite precipitation has received less attention. In this study, microbial communities of carbonate precipitates from drip waters in Nerja, a show cave close to the sea in southeastern Spain, were investigated. We observed a pronounced difference in the bacterial composition of the precipitates, depending on the galleries and halls. The most abundant phylum in the precipitates of the halls close to the cave entrance was Proteobacteria, due to the low depth of this sector, the direct influence of a garden on the top soil and the infiltration of waters into the cave, as well as the abundance of members of the order Hyphomicrobiales, dispersing from plant roots, and other Betaproteobacteria and Gammaproteobacteria, common soil inhabitants. The influence of marine aerosols explained the presence of Marinobacter, Idiomarina, Thala...
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The present study reports a sudden fungal outbreak that occurred in the corridor near the entrance of the Catacombs of SS. Marcellino and Pietro in Rome (Italy) observed after 1 year of a restoration treatment that interested the walls of... more
The present study reports a sudden fungal outbreak that occurred in the corridor near the entrance of the Catacombs of SS. Marcellino and Pietro in Rome (Italy) observed after 1 year of a restoration treatment that interested the walls of the entrance of the Catacombs and some artifacts placed in situ. The colonization was observed on the vault at the entrance and in correspondence with the restored marble pieces displayed on the left side of the corridor. No growth was observed on the right side where similarly treated marble slabs were placed. Samples taken in correspondence with fungal biofilm were analyzed through the combined use of microscopical, cultural, and molecular tools and showed that the vault and the left side of the corridor entrance were colonized by a complex fungal biofilm consisting mainly of Coniophora sp. and other genera, such as Hypomyces, Purpureocillium, Acremonium, Penicillium, and Alternaria, many of which are well known as responsible of biodeterioration...
Research Interests: Penicillium and Outbreak
Antimicrobial resistance has become a global problem in recent decades. A gradual reduction in drug discoveries has led to the current antimicrobial resistance crisis. Caves and other subsurface environments are underexplored thus far,... more
Antimicrobial resistance has become a global problem in recent decades. A gradual reduction in drug discoveries has led to the current antimicrobial resistance crisis. Caves and other subsurface environments are underexplored thus far, and they represent indispensable ecological niches that could offer new molecules of interest to medicine and biotechnology. We explored Spanish show caves to test the bioactivity of the bacteria dwelling in the walls and ceilings, as well as airborne bacteria. We reported the isolation of two strains of the genus Crossiella, likely representing a new species, isolated from Altamira Cave, Spain. In vitro and in silico analyses showed the inhibition of pathogenic Gram-positive and Gram-negative bacteria, and fungi, as well as the taxonomical distance of both strains from their closest relative, Crossiella cryophila. The presence of an exclusive combination of gene clusters involved in the synthesis of lanthipeptides, lasso peptides, nonribosomal peptid...
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Castañar Cave is a clear example of an oligotrophic ecosystem with high hygrothermal stability both seasonal and interannual and the particularity of registering extraordinary levels of environmental radiation. These environmental... more
Castañar Cave is a clear example of an oligotrophic ecosystem with high hygrothermal stability both seasonal and interannual and the particularity of registering extraordinary levels of environmental radiation. These environmental conditions make the cave an ideal laboratory to evaluate both the responses of the subterranean environment to sudden changes in the matter and energy fluxes with the exterior and also any impact derived from its use as a tourist resource under a very restrictive access regime. In 2008, a fungal outbreak provoked by a vomit contaminated the sediments which were removed and subsequently treated with hydrogen peroxide. Fungal surveys were carried out in 2008 and 2009. The visits were resumed in 2014. Here, 12 years after the outbreak, we present an exhaustive study on the cave sediments in order to know the distribution of the different fungal taxa, as well as the prevalence and spatio-temporal evolution of the fungi caused by the vomit over the years under ...
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Altamira, Lascaux and many other caves house some of the world¿s most prominent Paleolithicrock art. Open to the visitors for long time, some of these caves had to be closed to the public after decades of visits due to microbial outbreaks... more
Altamira, Lascaux and many other caves house some of the world¿s most prominent Paleolithicrock art. Open to the visitors for long time, some of these caves had to be closed to the public after decades of visits due to microbial outbreaks and severe deterioration of the paintings. Once closed to visits, the tourists were rerouted to replicas of Altamira and Lascaux paintings, however politics and public pressures still exist for a reopening, and Altamira still allows the visits on Friday. In September 2002, Altamira Cave had to be closed to the public because of the presence of phototrophic microorganisms on the famous bisons decorating the ceiling of the Polychrome Hall, a phenomenon similar to that suffered by Lascaux 50 years before. Colonization by phototrophic microorganisms was a consequence of decades of use of artificial lighting in the Polychrome Hall and was accompanied by the development of white microbial colonizations, directly on the red paintings. Lascaux Cave was closed in 1963, and after 2001 suffered several fungal outbreaks due to a wrong management and the use of benzalkonium chloride as biocide for controlling the fungi. A research carried out by the CSIC showed that the walls and ceiling of Altamira Cave were colonized by bacteria, but this contrasted with the absence of visible fungal development. It was suggested that the microbial communities colonizing the walls plays an important role in the ecosystem and the presence of antifungal compounds produced by the bacteria would protect the cave rock surfaces from fungal colonization. In fact, some species of actinobacteria found in Altamira Cave, such as Actinomadura, Amycolatopsis, Nocardia, Micromonospora, Rhodococcus, Streptomyces, etc., have antifungal activities
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El libro contiene las comunicaciones y posters presentados en el The third International congress of Science and Technology for the Conservation of Cultural Heritage, TechnoHeritage 2017, was held in Cadiz, from 21 to 24 May 2017, under... more
El libro contiene las comunicaciones y posters presentados en el The third International congress of Science and Technology for the Conservation of Cultural Heritage, TechnoHeritage 2017, was held in Cadiz, from 21 to 24 May 2017, under the umbrella of the TechnoHerita
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9 páginas.-- 6 figuras.-- 20 referencias[EN]: In recent years caves hove revealed to be a source of new microbial species. The great adaptability o( microorganisms al/ow them establishing themselves in these subterranean environments... more
9 páginas.-- 6 figuras.-- 20 referencias[EN]: In recent years caves hove revealed to be a source of new microbial species. The great adaptability o( microorganisms al/ow them establishing themselves in these subterranean environments where they develop different cammunities. Cueva del Tesoro (Rincón de la Victoria, Mólaga) harbours a great biodiversity barely explored. Recently, we hove described two new fungal species of the genus Aspergil/us. Three novel bacterial species of the genera Acinetabader, Bacil/us and Paracaccus are under research. The colomzatians of cyanobacteria and algae do requiere (urther investigation. These data support that the high biodiversity of Cueva del Tesoro should be preserved if it does not represent a threot for the conservation of the Cultural Heritage. [ES]: En los últimos años las cuevas se han revelado como una fuente de nuevas especies de microorganismos. La gran adaptación de los microorganismos a todo tipo de hábltats les permite vivir en estos ambientes donde se desarrollan formando diferentes comunidades. La Cueva del Tesoro (Rincón de la Victoria, Málaga) alberga una gran biodiversidad apenas explorada . Recientemente, hemos descrito dos nuevas especies de hongos del género Aspergil/us, y tres posibles nuevas especies de bacterias de los géneros Acinetobacter, Bacil/us y Paracoccus están siendo investigadas. Por otra parte, las colonizaciones de cianobacterias y algas requieren una investigación más detallada. Estos datos demuestran que la diversidad biológica de la Cueva del Tesoro es muy alta, por lo que debería ser preservada, siempre que no represente una amenaza para la conservación del Patrimonio Cultural.Este proyecto ha sido financiado por el proyecto de Excelencia de la Junta de Andalucía, código RNM5137 yun contrato del Excmo. Ayuntamiento del Rincón de la Victori a, MálagaPeer Reviewe
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41 páginas.-- 32 figuras.-- 24 tablas.--. 20 referenciasLa Cueva de Nerja, un Bien de Interés Cultural, es una de las cuevas turísticas más importantes en España, con 485.541 visitantes anuales durante el periodo 1988-2013 (Rosal del et... more
41 páginas.-- 32 figuras.-- 24 tablas.--. 20 referenciasLa Cueva de Nerja, un Bien de Interés Cultural, es una de las cuevas turísticas más importantes en España, con 485.541 visitantes anuales durante el periodo 1988-2013 (Rosal del et al., 2014). La cueva tiene dos torcas que pemúten la entrada de luz natural en la sala donde se encuentran, mientras que a lo largo del recorrido de sus galerías turísticas tiene una instalación de iluminación artificial, llevada a cabo en el año 1959, cuando se adaptó para las visitas turísticas. La presencia de luz, ya sea natural o artificial, desencadena el desarrollo de microorganismos fototróficos (cianobacterias y microalgas) sobre los espeleotemas y paredes. Estos microorganismos crecen formando biofilms que se adaptan a las condiciones ambientales imperantes en la cueva.Peer reviewe
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Natural precipitates of metastable polymorphs of CaCO3, such as vaterite, are rarely found in nature. However, spherical bodies composed of vaterite have been recognized in submilimetricthick microbial mats adn biofilms on volcanic... more
Natural precipitates of metastable polymorphs of CaCO3, such as vaterite, are rarely found in nature. However, spherical bodies composed of vaterite have been recognized in submilimetricthick microbial mats adn biofilms on volcanic substrates (St. Calixtinus Catacombs, Rome, Italy). Vaterite is formed beneath dense biofilms, where particular physicochemical conditions are produced by the microbial activity.This work was supported by the European Commission, EVK4-CT-2000-00028 (CATS).Peer reviewe
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Pseudomonas sp. strain L5B5 is an antimicrobial-producing bacterium isolated from an air sample collected in a pyrite mine in Lousal, Portugal. Genomic analyses predicted genes involved in virulence factors. Here, we report the complete... more
Pseudomonas sp. strain L5B5 is an antimicrobial-producing bacterium isolated from an air sample collected in a pyrite mine in Lousal, Portugal. Genomic analyses predicted genes involved in virulence factors. Here, we report the complete genome sequence of this bacterium, which consists of a circular chromosome with a length of 6,811,662 bp.
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Subsurface granitic environments are scarce and poorly investigated. A multi‐disciplinary approach was used to characterize the abundant moonmilk deposits and associated microbial communities coating the granite walls of the 16th Century... more
Subsurface granitic environments are scarce and poorly investigated. A multi‐disciplinary approach was used to characterize the abundant moonmilk deposits and associated microbial communities coating the granite walls of the 16th Century Paranhos spring water tunnel in Porto city (north‐west Portugal). It is possible that this study is the first record of moonmilk in an urban subsurface granitic environment. The morphology and texture, mineralogical composition, stable isotope composition and microbial diversity of moonmilk deposits have been studied to infer the processes of moonmilk formation. These whitish secondary mineral deposits are composed of very fine needle‐fibre calcite crystals with different morphologies and density. Calcified filaments of fungal hyphae or bacteria were distinguished by field emission scanning electron microscopy. Stable isotope analysis revealed a meteoric origin of the needle‐fibre calcite, with an important contribution of atmospheric CO2, soil resp...
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6 p.-- Papers presented at the International Workshop "The Conservation of Subterranean Cultural Heritage", held 25-27 March 2014, in Seville, Spain.
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11 páginas.-- 2 figuras.-- 40 referencias.--[EN]: Gruta de las Maravillas (Aracena, Huelva) is a cavity o( geological interest, with an important annual regime o( visits. We carried out a seasonal study o( the cave's aerobiology with... more
11 páginas.-- 2 figuras.-- 40 referencias.--[EN]: Gruta de las Maravillas (Aracena, Huelva) is a cavity o( geological interest, with an important annual regime o( visits. We carried out a seasonal study o( the cave's aerobiology with the aim o( knowing the concentration and distriblltion o( bacterial and (ungal communities, and determining their seasonal pattern and visitors' impacto The analysis showed that while some species o(bacteria are present along the (our seasons, such as Micrococcus yunnanensis, the (ungi present a higher variability, which means that there is not a predominant species. This study ollows us to know the air quality and to compare these results with the ones previollsly obtained in other touristic caves.[ES]: La Gruta de las Maravillas (Aracena, Hu elva) es una cavidad de interés geológico, que mantiene un rég imen anual de visitantes importante. Se consideró de interés realizar un estudio estacional de su aerobiología con el objetivo de conocer la concentración y distribución de las comunidades bacterianas y fúngicas presentes en la cueva, determinar su pauta estacional y el impacto de las visitas. Los análisis realizados indican que mientras algunas especies están presentes a lo largo de las cuatro estaciones del año, como la bacteria Microcaccus yunnanensis, los hongos presentan mayor variabilidad no existiendo una especie predominante. Este estudio permite conocer la calidad del aire y comparar los resultados con los obtenidos previamente en otras cuevas visitablesEste proyecto ha sido cofinanciado por el programa operativo FEDER de Andalucía 2007-2013, y por el Proyecto de Excelencia de la Junta de Andalucía, código RNM5137. La participación de CSJ y VJ es a través del proyecto CSIC 201230E125Peer reviewe
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The archaeological site of the Garma (Cantabria) is part of an active karst system with several levels of galleries interconnected by vertical shafts. La Garma houses an exceptional archaeological heritage that includes magnificent examS.... more
The archaeological site of the Garma (Cantabria) is part of an active karst system with several levels of galleries interconnected by vertical shafts. La Garma houses an exceptional archaeological heritage that includes magnificent examS. Cuezva, et al. 2016. Investigación aplicada a la conservación preventiva del sistema kárstico de La Garma (Omoño, Ribamontán al Monte... 308 ples of Palaeolithic cave art and settlement floors in an amazing state of preservation, as well as valuable evidence of symbolic behavior of Pleistocene hunter-gatherers. Its heritage values have made it worthy of their inclusion on the World Heritage List of UNESCO in 2008. The conservation of this site is a priority and it has been recently launched a program of preventive conservation. The main objective of this project is to achieve a better knowledge of the underground ecosystem and the impact of human presence in the underground microenvironment, both for purposes of sound management and research of the cavity as well as for a hypothetical opening to the public in the future. The underground karst system of La Garma, with various levels of galleries and directly connected with active water table, involves a complex movement of matter (water, air masses and aerosols) and energy. To achieve an adequate knowledge of environmental dynamics, our research group is conducting a multidisciplinary study since February 2015 by employing specific methodologies for each interface involved: microclimate (cavity in three internal levels of karst and external atmosphere), hydrochemical (water infiltration and condensation), isotope geochemistry (air, soil) and microbiological contamination (air, soil, rock). Early data show very marked seasonal changes in the general ventilation pattern of the cavity (air renewal in winter) and areas with different microenvironmental stability, including sectors with a greater degree of isolation from the regime of general circulation of air in the cavity.
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7 p.-- Papers presented at the International Workshop "The Conservation of Subterranean Cultural Heritage", held 25-27 March 2014, in Seville, Spain.
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Comunicacion presentada en la citada 2a Reunion: Conservacion del Patrimonio y conocimiento historico, celebrada el 17 de diciembre, 2002, en Madrid.
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European caves contain some of the world’s greatest Paleolithic paintings, and their conservation is at risk due to the use of artificial lighting. Both lighting and high CO2 promotes the growth of phototrophic organisms on walls,... more
European caves contain some of the world’s greatest Paleolithic paintings, and their conservation is at risk due to the use of artificial lighting. Both lighting and high CO2 promotes the growth of phototrophic organisms on walls, speleothems and ground sediments. In addition, the combined effect of increases in CO2, vapor concentration and temperature variations induced by visitors can directly affect the development of corrosion processes on the cave rock surfaces. An early detection of the occurrence of phototrophic biofilms on Paleolithic paintings is of the utmost importance, as well as knowing the microorganisms involved in the colonization of rocks and walls. Knowledge of the colonizing species and their ecology will allow the adoption of control measures. However, this is not always possible due to the limited amount of biomass available for molecular analyses. Here, we present an alternative approach to study faint green biofilms of Chlorophyta in the initial stage of colon...
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Show caves have different grades of colonization by phototrophic biofilms. They may receive a varied number of visits, from a few thousand to hundreds of thousands of visitors annually. Among them, Tesoro Cave, Rincon de la Victoria,... more
Show caves have different grades of colonization by phototrophic biofilms. They may receive a varied number of visits, from a few thousand to hundreds of thousands of visitors annually. Among them, Tesoro Cave, Rincon de la Victoria, Spain, showed severe anthropic alterations, including artificial lighting. The most noticeable effect of the lighting was the growth of a dense phototrophic community of cyanobacteria, algae and bryophytes on the speleothems, walls and ground. The biofilms were dominated by the cyanobacterium Phormidium sp., the chlorophyte Myrmecia israelensis, and the rhodophyte Cyanidium sp. In many cases, the biofilms also showed an abundance of the bryophyte Eucladium verticillatum. Other cyanobacteria observed in different biofilms along the cave were: Chroococcidiopsis sp., Synechocystis sp. and Nostoc cf. edaphicum, the green microalgae Pseudococcomyxa simplex, Chlorella sp. and the diatom Diadesmis contenta. Preliminary cleaning tests on selected areas showed t...
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The Cross of the Inquisition, sculpted in 1903 and raised on a column with a fluted shaft and ornamented with vegetable garlands, is located in a corner of the Plateresque façade of the Seville City Hall. The Cross was vandalized in... more
The Cross of the Inquisition, sculpted in 1903 and raised on a column with a fluted shaft and ornamented with vegetable garlands, is located in a corner of the Plateresque façade of the Seville City Hall. The Cross was vandalized in September 2019 and the restoration concluded in September 2021. A geological and microbiological study was carried out in a few small fragments. The data are consistent with the exposure of the Cross of the Inquisition to an urban environment for more than 100 years. During that time, a lichen community colonized the Cross and the nearby City Hall façades. The lichens, bryophytes and fungi colonizing the limestone surface composed an urban community, regenerated reorganized from the remains of the original communities, after superficial cleaning of the limestone between 2008 and 2010. This biological activity was detrimental to the integrity of the limestone, as showed by the pitting and channels, which evidence the lytic activity of organisms on the sto...
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Two Streptomyces strains, labeled as MZ03-37T and MZ03-48, were isolated from two different samples, a mucolite-type speleothem and a microbial mat on the walls of a lava tube from La Palma Island (Canary Islands). Phylogenetic analysis... more
Two Streptomyces strains, labeled as MZ03-37T and MZ03-48, were isolated from two different samples, a mucolite-type speleothem and a microbial mat on the walls of a lava tube from La Palma Island (Canary Islands). Phylogenetic analysis based on concatenated sequences of six housekeeping genes indicated that both strains belonged to the same species. The closest relatives for both strains were Streptomyces palmae CMU-AB204T (98.71%), Streptomyces catenulae NRRL B-2342T (98.35%), and Streptomyces ramulosus NRRL B-2714T (98.35%). Multi-locus sequence analysis (MLSA), based on five house-keeping gene alleles (i.e., atpD, gyrB, recA, rpoB, and trpB), indicated that both isolated strains were closely related to S. catenulae NRRL B-2342T. Whole-genome average nucleotide identity (ANI) scores of both strains were in the threshold value for species delineation with the closest species. Both strains presented a G+C content of 72.1 mol%. MZ03-37T was light brown in substrate and white in aeri...
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Subterranean ecosystems play an active role in the global carbon cycle, yet only a few studies using indirect methods have focused on the role of the cave microbiota in this critical cycle. Here we present pioneering research based on in... more
Subterranean ecosystems play an active role in the global carbon cycle, yet only a few studies using indirect methods have focused on the role of the cave microbiota in this critical cycle. Here we present pioneering research based on in situ real-time monitoring of CO2 and CH4 diffusive fluxes and concurrent δ13C geochemical tracing in caves, combined with 16S microbiome analysis. Our findings show that cave sediments are promoting continuous CH4 consumption from cave atmosphere, resulting in a significant removal of 65% to 90%. This research reveals the most effective taxa and metabolic pathways in consumption and uptake of greenhouse gases. Methanotrophic bacteria were the most effective group involved in CH4 consumption, namely within the families Methylomonaceae, Methylomirabilaceae and Methylacidiphilaceae. In addition, Crossiella and Nitrosococcaceae wb1-P19 could be one of the main responsible of CO2 uptake, which occurs via the Calvin-Benson-Bassham cycle and reversible hydration of CO2. Thus, syntrophic relationships exist between Crossiella and nitrifying bacteria that capture CO2, consume inorganic N produced by heterotrophic ammonification in the surface of sediments, and induce moonmilk formation. Moonmilk is found as the most evolved phase of the microbial processes in cave sediments that fixes CO2 as calcite and intensifies CH4 oxidation. From an ecological perspective, cave sediments act qualitatively as soils, providing fundamental ecosystem services (e.g. nutrient cycling and carbon sequestration) with direct influence on greenhouse gas emissions.
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Of the several critical challenges present in environmental microbiology today, one is the assessment of the contribution of microorganisms in the carbon cycle in the Earth-climate system. Karstic subterranean ecosystems have been... more
Of the several critical challenges present in environmental microbiology today, one is the assessment of the contribution of microorganisms in the carbon cycle in the Earth-climate system. Karstic subterranean ecosystems have been overlooked until recently. Covering up to 25% of the land surface and acting as a rapid CH4 sink and alternately as a CO2 source or sink, karstic subterranean ecosystems play a decisive role in the carbon cycle in terms of their contribution to the global balance of greenhouse gases. Recent data indicate that microbiota must play a significant ecological role in the biogeochemical processes that control the composition of the subterranean atmosphere, as well as in the availability of nutrients for the ecosystem. Nevertheless, there are still essential gaps in our knowledge concerning the budgets of greenhouse gases at the ecosystem scale and the possible feedback mechanisms between environmental-microclimatic conditions and the rates and type of activity o...
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El libro contiene las comunicaciones y posters presentados en el The third International congress of Science and Technology for the Conservation of Cultural Heritage, TechnoHeritage 2017, was held in Cadiz, from 21 to 24 May 2017, under... more
El libro contiene las comunicaciones y posters presentados en el The third International congress of Science and Technology for the Conservation of Cultural Heritage, TechnoHeritage 2017, was held in Cadiz, from 21 to 24 May 2017, under the umbrella of the TechnoHeritage network
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Comunicacion presentada en el International Workshop "The Conservation of Subterranean Cultural Heritage", held 25-27 March 2014, in Seville, Spain.-- The workshop is organized by the Spanish Network of Science and Technology... more
Comunicacion presentada en el International Workshop "The Conservation of Subterranean Cultural Heritage", held 25-27 March 2014, in Seville, Spain.-- The workshop is organized by the Spanish Network of Science and Technology for the Conservation of Cultural Heritage (TechnoHeritage)
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4 paginas.-- 3 figuras..- 18 referencias.-- Comunicacion presentada en el Congreso Estudio y Conservacion del Patrimonio Cultural. Actas 16-19 de noviembre 2015, Malaga
11 paginas.-- 2 figuras.-- 41 referencias.-- Conferencia presentada en el Sexto Congreso Espanol sobre Cuevas Turisticas, celebrado en Nerja (Malaga), en septiembre/octubre de 2016.-- Descarga de las Actas en... more
11 paginas.-- 2 figuras.-- 41 referencias.-- Conferencia presentada en el Sexto Congreso Espanol sobre Cuevas Turisticas, celebrado en Nerja (Malaga), en septiembre/octubre de 2016.-- Descarga de las Actas en http://www.cuevasturisticas.es/cuevatur/docs/2016/pdf/LibroCuevastur2016.pdf
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The aerobiology of caves in Southern Spain possesses special characteristics, different from caves located in Northern Spain. Previous studies demonstrated the influence of outdoor air on caves in the north and the existence of two... more
The aerobiology of caves in Southern Spain possesses special characteristics, different from caves located in Northern Spain. Previous studies demonstrated the influence of outdoor air on caves in the north and the existence of two different patterns, depending on the season. In summer there is an abundance of Ascomycota, whereas in winter Basidiomycota predominates, which are related to the periods of stagnation and ventilation, respectively. In caves in Southern Spain the presence of airborne Basidiomycota is scarce and Ascomycota represents the main group of fungi widely distributed across the caves in all seasons. The most characteristic features were the abundant presence of entomopathogenic fungi (Beauveria bassiana, Parengyodontium album, Pochonia chlamydosporia, Leptobacillium symbioticum, Leptobacillium leptobactrum) and Cladosporium cladosporioides in Cueva del Tesoro, Cueva de Ardales and Gruta de las Maravillas. However, the presence of yeasts of the genera Cutaneotricho...
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Morgana Cave is located in Val di Scerscen, Central Italian Alps. The cave opens at an altitude of 2,600 m a.s.l. close to the retreating glacier Vedretto di Scerscen, and its entrance was discovered 30 years ago hidden underneath the... more
Morgana Cave is located in Val di Scerscen, Central Italian Alps. The cave opens at an altitude of 2,600 m a.s.l. close to the retreating glacier Vedretto di Scerscen, and its entrance was discovered 30 years ago hidden underneath the glacier. A characteristic of this cave is the occurrence of vermiculation deposits on the walls and ceiling. In general, the composition of the microbial communities in cave vermiculations is relatively unknown and rarely investigated. Here we present the data of a geomicrobiological study of vermiculations from an Alpine cave subjected to extreme climate conditions. The microbial communities were dominated by 13 main phyla of Bacteria, and contained a negligible percentage (<1%) of Archaea. The two major bacterial classes were Gammaproteobacteria and Betaproteobacteria, whose metabolic traits were mainly associated with the nitrogen cycle. In addition, psychrophilic and methanotrophic bacterial groups were identified. The occurrence of a large numb...
Research Interests: Ecology, Nitrogen Cycle, Cave, Archaea, Bacteria, and 3 moreMicrobial Mat, Glacier, and Betaproteobacteria
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The archaeological site of the Garma (Cantabria) is part of an active karst system with several levels of galleries interconnected by vertical shafts. La Garma houses an exceptional archaeological heritage that includes magnificent examS.... more
The archaeological site of the Garma (Cantabria) is part of an active karst system with several levels of galleries interconnected
by vertical shafts. La Garma houses an exceptional archaeological heritage that includes magnificent examS.
Cuezva, et al. 2016. Investigación aplicada a la conservación preventiva del sistema kárstico de La Garma (Omoño, Ribamontán al Monte...
308
ples of Palaeolithic cave art and settlement floors in an amazing state of preservation, as well as valuable evidence of
symbolic behavior of Pleistocene hunter-gatherers. Its heritage values have made it worthy of their inclusion on the
World Heritage List of UNESCO in 2008. The conservation of this site is a priority and it has been recently launched
a program of preventive conservation. The main objective of this project is to achieve a better knowledge of the underground
ecosystem and the impact of human presence in the underground microenvironment, both for purposes
of sound management and research of the cavity as well as for a hypothetical opening to the public in the future.
The underground karst system of La Garma, with various levels of galleries and directly connected with active water
table, involves a complex movement of matter (water, air masses and aerosols) and energy. To achieve an adequate
knowledge of environmental dynamics, our research group is conducting a multidisciplinary study since February
2015 by employing specific methodologies for each interface involved: microclimate (cavity in three internal levels
of karst and external atmosphere), hydrochemical (water infiltration and condensation), isotope geochemistry (air,
soil) and microbiological contamination (air, soil, rock). Early data show very marked seasonal changes in the general
ventilation pattern of the cavity (air renewal in winter) and areas with different microenvironmental stability, including
sectors with a greater degree of isolation from the regime of general circulation of air in the cavity.
by vertical shafts. La Garma houses an exceptional archaeological heritage that includes magnificent examS.
Cuezva, et al. 2016. Investigación aplicada a la conservación preventiva del sistema kárstico de La Garma (Omoño, Ribamontán al Monte...
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ples of Palaeolithic cave art and settlement floors in an amazing state of preservation, as well as valuable evidence of
symbolic behavior of Pleistocene hunter-gatherers. Its heritage values have made it worthy of their inclusion on the
World Heritage List of UNESCO in 2008. The conservation of this site is a priority and it has been recently launched
a program of preventive conservation. The main objective of this project is to achieve a better knowledge of the underground
ecosystem and the impact of human presence in the underground microenvironment, both for purposes
of sound management and research of the cavity as well as for a hypothetical opening to the public in the future.
The underground karst system of La Garma, with various levels of galleries and directly connected with active water
table, involves a complex movement of matter (water, air masses and aerosols) and energy. To achieve an adequate
knowledge of environmental dynamics, our research group is conducting a multidisciplinary study since February
2015 by employing specific methodologies for each interface involved: microclimate (cavity in three internal levels
of karst and external atmosphere), hydrochemical (water infiltration and condensation), isotope geochemistry (air,
soil) and microbiological contamination (air, soil, rock). Early data show very marked seasonal changes in the general
ventilation pattern of the cavity (air renewal in winter) and areas with different microenvironmental stability, including
sectors with a greater degree of isolation from the regime of general circulation of air in the cavity.