In this study, we investigated the impact of the host factors mucin, bile salts and cholesterol o... more In this study, we investigated the impact of the host factors mucin, bile salts and cholesterol on the virulence of the economically important aquatic pathogen Vibrio anguillarum towards sea bass larvae. Pretreatment of V. anguillarum with either one of the host factors (at 10 mg l(-1)) prior to inoculation into the sea bass rearing water increased virulence of the bacterium, although the effect of cholesterol was not significant. Each of the three host factors significantly increased several virulence-related phenotypes in V. anguillarum, i.e. protease activity, flagellar motility, biofilm formation and exopolysaccharide production, whereas there was no effect on growth of the bacterium under these conditions. Furthermore, the host factors increased the expression of genes involved in these phenotypes, i.e. the metalloprotease empA, the flagellar transcriptional regulator fleQ, the flagellin gene flaA, the chemotaxis methyltransferase gene cheR, the exopolysaccharide biosynthesis g...
Infections caused by antibiotic-resistant luminescent Vibrios can cause dramatic losses in aquacu... more Infections caused by antibiotic-resistant luminescent Vibrios can cause dramatic losses in aquaculture. In this study, the short-chain fatty acid beta-hydroxybutyrate and its polymer poly-beta-hydroxybutyrate were investigated as possible new biocontrol agents. beta-Hydroxybutyrate was shown to completely inhibit the growth of pathogenic Vibrio campbelli at 100 mM. Moreover, the addition of 100 mM of this fatty acid to the culture water of Artemia nauplii infected with the V. campbelli strain significantly increased the survival of the nauplii. As Artemia is a non-selective and particle filter feeder, we also investigated whether poly-beta-hydroxybutyrate particles could be used to protect Artemia from the pathogenic V. campbellii. The addition of 100 mg l(-1) poly-beta-hydroxybutyrate or more to the Artemia culture water offered a preventive and curative protection from the pathogen as a significantly enhanced survival was noticed. If added as a preventive treatment, a complete pro...
... England. Other Details: Languages: eng Pagination: 36 Citation Subset: IM. Affiliation: Depar... more ... England. Other Details: Languages: eng Pagination: 36 Citation Subset: IM. Affiliation: Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital,'s-Hertogenbosh, The Netherlands. colin.ingham@wur.nl. ...
Vlaams Instituut voor de Zee. VLIZ. Informatie over marien en kustgebonden onderzoek & be... more Vlaams Instituut voor de Zee. VLIZ. Informatie over marien en kustgebonden onderzoek & beleid in Vlaanderen.
In this study, we investigated the impact of the host factors mucin, bile salts and cholesterol o... more In this study, we investigated the impact of the host factors mucin, bile salts and cholesterol on the virulence of the economically important aquatic pathogen Vibrio anguillarum towards sea bass larvae. Pretreatment of V. anguillarum with either one of the host factors (at 10 mg l(-1)) prior to inoculation into the sea bass rearing water increased virulence of the bacterium, although the effect of cholesterol was not significant. Each of the three host factors significantly increased several virulence-related phenotypes in V. anguillarum, i.e. protease activity, flagellar motility, biofilm formation and exopolysaccharide production, whereas there was no effect on growth of the bacterium under these conditions. Furthermore, the host factors increased the expression of genes involved in these phenotypes, i.e. the metalloprotease empA, the flagellar transcriptional regulator fleQ, the flagellin gene flaA, the chemotaxis methyltransferase gene cheR, the exopolysaccharide biosynthesis g...
Infections caused by antibiotic-resistant luminescent Vibrios can cause dramatic losses in aquacu... more Infections caused by antibiotic-resistant luminescent Vibrios can cause dramatic losses in aquaculture. In this study, the short-chain fatty acid beta-hydroxybutyrate and its polymer poly-beta-hydroxybutyrate were investigated as possible new biocontrol agents. beta-Hydroxybutyrate was shown to completely inhibit the growth of pathogenic Vibrio campbelli at 100 mM. Moreover, the addition of 100 mM of this fatty acid to the culture water of Artemia nauplii infected with the V. campbelli strain significantly increased the survival of the nauplii. As Artemia is a non-selective and particle filter feeder, we also investigated whether poly-beta-hydroxybutyrate particles could be used to protect Artemia from the pathogenic V. campbellii. The addition of 100 mg l(-1) poly-beta-hydroxybutyrate or more to the Artemia culture water offered a preventive and curative protection from the pathogen as a significantly enhanced survival was noticed. If added as a preventive treatment, a complete pro...
... England. Other Details: Languages: eng Pagination: 36 Citation Subset: IM. Affiliation: Depar... more ... England. Other Details: Languages: eng Pagination: 36 Citation Subset: IM. Affiliation: Department of Medical Microbiology and Infection Control, Jeroen Bosch Hospital,'s-Hertogenbosh, The Netherlands. colin.ingham@wur.nl. ...
Vlaams Instituut voor de Zee. VLIZ. Informatie over marien en kustgebonden onderzoek & be... more Vlaams Instituut voor de Zee. VLIZ. Informatie over marien en kustgebonden onderzoek & beleid in Vlaanderen.
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