T. Masjudin

To compare the efficacy of autologous chondrocyte transplantation (ACT) versus non-operative measures for cartilage repair in rabbits. Nine New Zealand white rabbits were used. Identical focal defects were created in the articular cartilage of both knees. One month later, the right knee was repaired via ACT, while the left knee was left untreated (control group). The quality of cartilage tissues in both knees was compared 3 months later, according to the quantitative analysis of glycosaminoglycan (GAG) in the cartilage and macroscopic examination of histology using the Brittberg/International Cartilage Research Society (ICRS) score. Microscopic examination showed enhanced regeneration following ACT repair. Quantification analysis revealed significantly higher cellular expression of GAG in the ACT-treated knees (1.12 vs 0.81 microgram GAGs/mg protein, p=0.008). The mean Brittberg/ICRS score was significantly higher in the treated knees (6.00 vs 1.89, p=0.007). ACT is superior to non-...

This prospective randomised study was undertaken to compare surgical parameters as well as clinical and radiological outcomes of subvastus (SV) to midvastus (MV) approaches in staged bilateral total knee arthroplasty (TKA). Twenty-three patients, aged 55-76 years, who underwent staged bilateral TKAs, were followed up for 6 months. The SV approach was used on one knee and MV approach on the other. We found similar lateral retinacular release rates and patellar resurfacing rates between the two approaches .The SV approached knees had a more significant blood loss and increased operative time compared to the MV approached knees but they achieved significantly earlier active straightleg raises (SLR) postoperatively (p<0.05 for all). The average postoperative pain, flexion, and Knee Society scores were comparable for both approaches. TKA via SV vs. MV approaches provides comparable satisfactory short-term clinical and radiological outcomes, even though there was slightly more difficul...

To evaluate the ability of autologous chondrocyte transplantation to repair articular cartilage defects, autologous chondrocytes embedded in alginate beads were implanted in focal cartilage defects created in 9 New Zealand white rabbits. After 4 weeks of cartilage damage, the right knee was repaired via autologous chondrocyte-alginate constructs transplantation and the left knee was left untreated (control group). The quality of cartilage tissues of both knees was then compared at 3 months following the procedure, as reflected by the ...

[摘要]: 正 Articular cartilage lesion repair remains a challenging problem due to its avascular, aneural and alymphatic properties resulting in poor regenerative ability. Autologous chondrocyte transplantation (ACT), a cell-based therapy, has provided a new perspective in articular cartilage management however its translation for lab based models to clinical application needs to be evaluated to ensure safety and efficacy issues are addressed in human transplantations. In a series of experiments from in vivo animal trials to clinical pilot ...