Journal of the Science of Food and Agriculture, 2015
BACKGROUNDPropolis is a relevant research subject worldwide. However, there is no information so ... more BACKGROUNDPropolis is a relevant research subject worldwide. However, there is no information so far on Bolivian propolis. Ten propolis samples were collected from regions with high biodiversity in the main honey production places in Bolivia and were analyzed for their total phenolics (TP), flavonoids (TF) and antioxidant activity. The chemical profiles of the samples were assessed by TLC, HPLC‐DAD, HPLC‐DAD‐MS/MSn and NMR analysis.RESULTSTP, TF, TLC and NMR analysis showed significant chemical differences between the samples. Isolation of the main constituents by chromatography and identification by HPLC‐DAD‐MS/MSn achieved more than 35 constituents. According to their profiles, the Bolivian propolis can be classified into phenolic‐rich and triterpene‐rich samples. Propolis from the valleys (Cochabamba, Chuquisaca and Tarija) contained mainly prenylated phenylpropanoids, while samples from La Paz and Santa Cruz contained cycloartane and pentacyclic triterpenes. Phenolic‐rich sample...
Due to the high prevalence of gastrointestinal nematodes in sheep, the growing anthelmintic resis... more Due to the high prevalence of gastrointestinal nematodes in sheep, the growing anthelmintic resistance, and the development of organic farming systems, sustainable alternatives are being sought. One such method is phytotherapy. This study aimed to evaluate the in vitro ovicidal and larvicidal activity of extracts of tansy (Tanacetum vulgare L.) growing in Latvia on gastrointestinal nematodes (Trichostrongylidae) in sheep. The leaves and flowers of the tansy were extracted separately in 70%, 50%, and 30% ethanol and acetone. Six concentrations were prepared from each extract 500 mg/mL, 200 mg/mL, 100 mg/mL, 50 mg/mL, 20 mg/mL, and 10 mg/mL. In vitro egg hatching test and micro-agar larval development test were performed. Extracts of tansy have strong larvicidal activity. The highest percentage of larvae inhibition for most of the extracts was 100%, but for egg inhibition, it was 95.8% for the 200 mg/mL concentration of 50% acetone and 93.3% for the 500 mg/mL concentration of 50% etha...
A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternativ... more A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternatively, herbal medicine has been proposed due to bacterial resistance. The aim of this study was to determine the antibacterial activity of the acetonic and ethanolic extracts of dried flowers and leaves of Tanacetum vulgare L. against bovine mastitis-inducing clinical isolates such as Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and reference cultures of S. aureus and E. coli. The extracts of T. vulgare showed partial antibacterial activity against tested strains of S. aureus. The MIC and MBC values of a 70% ethanol extract of flowers (MIC = 3.4 mg/mL, MBC = 3.4–6.8 mg/mL) were lower than for the 70% ethanol extract of leaves (MIC = 15.7–31.4 mg/mL, MBC = 62.9–125.9 mg/mL). The flower extracts showed low activity against E. coli (MIC = 53.9 mg/mL, MBC = 53.9–107.8 mg/mL) and S. agalactiae (MIC, MBC = 53.9 mg/mL). T. vulgare...
SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference, 2021
Nowadays, the provision of pharmaceutical care and effective communication with customers are bei... more Nowadays, the provision of pharmaceutical care and effective communication with customers are being discussed more often. A pharmacist is a specialist in his field with specific knowledge in the field of pharmacy. Therefore, the trust from the customer’s side and a kind, informative, and professional approach from the pharmacist’s side are very important in this case. Objective: To clarify what knowledge do the pharmacy customers have about pharmaceutical care and how satisfied they are with the pharmaceutical care services. The research was conducted using a quantitative survey, the statistical analysis of the obtained data was carried out using data processing software Microsoft Office Excel 2010, 267 respondents from various regions of Latvia were surveyed. Only 37% of respondents understand meaning of pharmaceutical care. This means that most of the customers are unaware of the duties and responsibilities of a pharmacist. Most of the respondents associate pharmaceutical care wit...
SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference, 2020
Over fifteen years, polypharmacy has increased by 4.9% across all age groups. Consequently, the r... more Over fifteen years, polypharmacy has increased by 4.9% across all age groups. Consequently, the responsibility of professionals for advising patients in such cases has increased as well. The research aims at studying patients' habits when purchasing and using medicinal products to enhance the theoretical and practical training of assistant pharmacists at the RSU Red Cross Medical College. The research is based on the patient questionnaires and further analysis of results within a focus group. 404 respondents were interviewed between December 2017 and November 2019. Under the conventions of social studies, this ensured a 3% margin of error at a confidence level of 5%. According to the results of the questionnaire, 44% of the surveyed women and 66% of men were rarely or very rarely interested in the possible side effects of a new medicinal products, food supplement or herbal product. Patients could obtain such information by reading the package leaflet, however, 24% of women and 4...
Background and Aim: Bovine mastitis has a negative impact on animals, and improper antibiotic use... more Background and Aim: Bovine mastitis has a negative impact on animals, and improper antibiotic use has caused an increase in bacterial resistance. Therefore, medicinal plants could serve as an alternative treatment for this condition. Polyphenols have potential as antibiotic agents. Oak bark has long been used as a medicine and has shown antibacterial effects. Moreover, research on heather plant demonstrated that it has antibacterial properties. This study aimed to assess the antibacterial effects of oak (Quercus robur) bark and heather (Calluna vulgaris L.) herb extracts against common bovine mastitis pathogens. Materials and Methods: Dried oak bark and heather herb were used to prepare extracts using 30%, 50%, and 70% ethanol and acetone as solvents. Their polyphenol content was determined using the Folin–Ciocalteu method. Bovine mastitis-inducing clinical isolates of Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and...
Numerous treatment agents offering prophylaxis against livestock parasites are commercially avail... more Numerous treatment agents offering prophylaxis against livestock parasites are commercially available. However, because of increasing antiparasitic drug resistance, the increased popularity of environmentally friendly lifestyle choices, and organic farming, there is more demand for new alternatives to livestock anthelmintic control strategies and medications. It is important to develop antiparasitics that are safe, effective, inexpensive, and environmentally safe. Local, traditional herbal plants such as tansy, mugwort, wormwood, and heather may serve as treatments for intestinal parasites of sheep. This overview provides knowledge of traditional Latvian plants with antiparasitic activities to establish a database for further research to develop new herbal antiparasitic drugs.
Numerous treatment agents offering prophylaxis against livestock parasites are commercially avail... more Numerous treatment agents offering prophylaxis against livestock parasites are commercially available. However, because of increasing antiparasitic drug resistance, the increased popularity of environmentally friendly lifestyle choices, and organic farming, there is more demand for new alternatives to livestock anthelmintic control strategies and medications. It is important to develop antiparasitics that are safe, effective, inexpensive, and environmentally safe. Local, traditional herbal plants such as tansy, mugwort, wormwood, and heather may serve as treatments for intestinal parasites of sheep. This overview provides knowledge of traditional Latvian plants with antiparasitic activities to establish a database for further research to develop new herbal antiparasitic drugs.
Journal of the Science of Food and Agriculture, 2015
BACKGROUNDPropolis is a relevant research subject worldwide. However, there is no information so ... more BACKGROUNDPropolis is a relevant research subject worldwide. However, there is no information so far on Bolivian propolis. Ten propolis samples were collected from regions with high biodiversity in the main honey production places in Bolivia and were analyzed for their total phenolics (TP), flavonoids (TF) and antioxidant activity. The chemical profiles of the samples were assessed by TLC, HPLC‐DAD, HPLC‐DAD‐MS/MSn and NMR analysis.RESULTSTP, TF, TLC and NMR analysis showed significant chemical differences between the samples. Isolation of the main constituents by chromatography and identification by HPLC‐DAD‐MS/MSn achieved more than 35 constituents. According to their profiles, the Bolivian propolis can be classified into phenolic‐rich and triterpene‐rich samples. Propolis from the valleys (Cochabamba, Chuquisaca and Tarija) contained mainly prenylated phenylpropanoids, while samples from La Paz and Santa Cruz contained cycloartane and pentacyclic triterpenes. Phenolic‐rich sample...
Due to the high prevalence of gastrointestinal nematodes in sheep, the growing anthelmintic resis... more Due to the high prevalence of gastrointestinal nematodes in sheep, the growing anthelmintic resistance, and the development of organic farming systems, sustainable alternatives are being sought. One such method is phytotherapy. This study aimed to evaluate the in vitro ovicidal and larvicidal activity of extracts of tansy (Tanacetum vulgare L.) growing in Latvia on gastrointestinal nematodes (Trichostrongylidae) in sheep. The leaves and flowers of the tansy were extracted separately in 70%, 50%, and 30% ethanol and acetone. Six concentrations were prepared from each extract 500 mg/mL, 200 mg/mL, 100 mg/mL, 50 mg/mL, 20 mg/mL, and 10 mg/mL. In vitro egg hatching test and micro-agar larval development test were performed. Extracts of tansy have strong larvicidal activity. The highest percentage of larvae inhibition for most of the extracts was 100%, but for egg inhibition, it was 95.8% for the 200 mg/mL concentration of 50% acetone and 93.3% for the 500 mg/mL concentration of 50% etha...
A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternativ... more A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternatively, herbal medicine has been proposed due to bacterial resistance. The aim of this study was to determine the antibacterial activity of the acetonic and ethanolic extracts of dried flowers and leaves of Tanacetum vulgare L. against bovine mastitis-inducing clinical isolates such as Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and reference cultures of S. aureus and E. coli. The extracts of T. vulgare showed partial antibacterial activity against tested strains of S. aureus. The MIC and MBC values of a 70% ethanol extract of flowers (MIC = 3.4 mg/mL, MBC = 3.4–6.8 mg/mL) were lower than for the 70% ethanol extract of leaves (MIC = 15.7–31.4 mg/mL, MBC = 62.9–125.9 mg/mL). The flower extracts showed low activity against E. coli (MIC = 53.9 mg/mL, MBC = 53.9–107.8 mg/mL) and S. agalactiae (MIC, MBC = 53.9 mg/mL). T. vulgare...
SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference, 2021
Nowadays, the provision of pharmaceutical care and effective communication with customers are bei... more Nowadays, the provision of pharmaceutical care and effective communication with customers are being discussed more often. A pharmacist is a specialist in his field with specific knowledge in the field of pharmacy. Therefore, the trust from the customer’s side and a kind, informative, and professional approach from the pharmacist’s side are very important in this case. Objective: To clarify what knowledge do the pharmacy customers have about pharmaceutical care and how satisfied they are with the pharmaceutical care services. The research was conducted using a quantitative survey, the statistical analysis of the obtained data was carried out using data processing software Microsoft Office Excel 2010, 267 respondents from various regions of Latvia were surveyed. Only 37% of respondents understand meaning of pharmaceutical care. This means that most of the customers are unaware of the duties and responsibilities of a pharmacist. Most of the respondents associate pharmaceutical care wit...
SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference, 2020
Over fifteen years, polypharmacy has increased by 4.9% across all age groups. Consequently, the r... more Over fifteen years, polypharmacy has increased by 4.9% across all age groups. Consequently, the responsibility of professionals for advising patients in such cases has increased as well. The research aims at studying patients' habits when purchasing and using medicinal products to enhance the theoretical and practical training of assistant pharmacists at the RSU Red Cross Medical College. The research is based on the patient questionnaires and further analysis of results within a focus group. 404 respondents were interviewed between December 2017 and November 2019. Under the conventions of social studies, this ensured a 3% margin of error at a confidence level of 5%. According to the results of the questionnaire, 44% of the surveyed women and 66% of men were rarely or very rarely interested in the possible side effects of a new medicinal products, food supplement or herbal product. Patients could obtain such information by reading the package leaflet, however, 24% of women and 4...
Background and Aim: Bovine mastitis has a negative impact on animals, and improper antibiotic use... more Background and Aim: Bovine mastitis has a negative impact on animals, and improper antibiotic use has caused an increase in bacterial resistance. Therefore, medicinal plants could serve as an alternative treatment for this condition. Polyphenols have potential as antibiotic agents. Oak bark has long been used as a medicine and has shown antibacterial effects. Moreover, research on heather plant demonstrated that it has antibacterial properties. This study aimed to assess the antibacterial effects of oak (Quercus robur) bark and heather (Calluna vulgaris L.) herb extracts against common bovine mastitis pathogens. Materials and Methods: Dried oak bark and heather herb were used to prepare extracts using 30%, 50%, and 70% ethanol and acetone as solvents. Their polyphenol content was determined using the Folin–Ciocalteu method. Bovine mastitis-inducing clinical isolates of Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and...
Numerous treatment agents offering prophylaxis against livestock parasites are commercially avail... more Numerous treatment agents offering prophylaxis against livestock parasites are commercially available. However, because of increasing antiparasitic drug resistance, the increased popularity of environmentally friendly lifestyle choices, and organic farming, there is more demand for new alternatives to livestock anthelmintic control strategies and medications. It is important to develop antiparasitics that are safe, effective, inexpensive, and environmentally safe. Local, traditional herbal plants such as tansy, mugwort, wormwood, and heather may serve as treatments for intestinal parasites of sheep. This overview provides knowledge of traditional Latvian plants with antiparasitic activities to establish a database for further research to develop new herbal antiparasitic drugs.
Numerous treatment agents offering prophylaxis against livestock parasites are commercially avail... more Numerous treatment agents offering prophylaxis against livestock parasites are commercially available. However, because of increasing antiparasitic drug resistance, the increased popularity of environmentally friendly lifestyle choices, and organic farming, there is more demand for new alternatives to livestock anthelmintic control strategies and medications. It is important to develop antiparasitics that are safe, effective, inexpensive, and environmentally safe. Local, traditional herbal plants such as tansy, mugwort, wormwood, and heather may serve as treatments for intestinal parasites of sheep. This overview provides knowledge of traditional Latvian plants with antiparasitic activities to establish a database for further research to develop new herbal antiparasitic drugs.
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