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    Marijana Saric

    Dietas inadequadas demonstraram ser muito prejudiciais para a manutenção de uma boa saúde, e comer fast-food em excesso e refeições altamente processadas pode ter um impacto negativo no estado geral de saúde da população. Assim, este... more
    Dietas inadequadas demonstraram ser muito prejudiciais para a manutenção de uma boa saúde, e comer fast-food em excesso e refeições altamente processadas pode ter um impacto negativo no estado geral de saúde da população. Assim, este trabalho investigou os hábitos relacionados com a alimentação fora de casa e a frequência de refeições rápidas numa amostra oriunda de seis países diferentes. Foi realizado um estudo descritivo transversal numa amostra de 4904 participantes adultos. Para o tratamento dos dados, a análise estatística básica foi complementada com análise de classificação do tipo árvore (Tree), usando o algoritmo CRT. Este estudo permitiu concluir que na amostra em análise a frequência com que os participantes comeram fora de casa foi em geral baixa, correspondendo a quatro ou menos vezes por semana para 71,9% dos participantes, sendo o consumo de fast-food também aceitável, ou seja, com baixa frequência de até duas vezes por semana em 88,6% dos entrevistados. As maiores d...
    The main source of mercury (Hg) exposure in the general population is fish. Another possible source is dental amalgam. Here, we compare the levels of Hg and selenium (Se) in samples of maternal and fetal origin collected shortly after... more
    The main source of mercury (Hg) exposure in the general population is fish. Another possible source is dental amalgam. Here, we compare the levels of Hg and selenium (Se) in samples of maternal and fetal origin collected shortly after childbirth of healthy postpartum women in the coastal (n = 96) and continental (n = 185) areas of Croatia related to maternal seafood/fish consumption. We also evaluated Hg concentrations and maternal serum metallothionein (MT2) concentrations in relation to the number of dental amalgam fillings, and MT2A-5A/G (rs28366003) polymorphism. The levels of Hg and Se in maternal hair and blood/serum, placenta and cord blood/serum increased in relation to increasing fish consumption with the highest values in subjects from the coast. The concentrations of each element and between elements correlated across the matrices. Increasing amalgam number correlated linearly with increased Hg levels in maternal and cord serum and was not associated with serum MT2. No as...
    This paper discusses the physiology of sodium effects on calcium metabolism and possible implications of increased salt intake on bone remodelling and bone mass. Osteoporosis is an increasing public health problem affecting more than 200... more
    This paper discusses the physiology of sodium effects on calcium metabolism and possible implications of increased salt intake on bone remodelling and bone mass. Osteoporosis is an increasing public health problem affecting more than 200 million of women around the world. The major complications of osteoporosis are fractures, which are frequently associated with high morbidity and mortality. A number of clinical, epidemiological and experimental studies aim at identifying lifestyle factors that may improve bone mass and prevent bone loss. Different nutrients are proposed to play a role in bone development during growth and in the maintenance of bone mass thereafter. However, the importance of sodium intake for bone health has not been elucidated. It is well known that high dietary sodium intake decreases renal calcium reabsorption, which in turn leads to a greater urinary calcium excretion. This effect has been demonstrated in studies in humans of all ages as well as in experimental...
    There is compelling evidence that fruit, vegetables, whole cereals, and legumes make about 80 % of the total food fibre intake and have a potential to help in the prevention of a number of diseases. The aim of our study was to estimate... more
    There is compelling evidence that fruit, vegetables, whole cereals, and legumes make about 80 % of the total food fibre intake and have a potential to help in the prevention of a number of diseases. The aim of our study was to estimate total fibre intake from consumption of this fibre-rich food, partly reported in our earlier study in Croatian adult population. Current data analysis involved a non-probabilistic sample of 1,034 adult participants from Dalmatia, Croatia who responded to a validated food frequency questionnaire between October 2014 and March 2015. We also analysed the sales data obtained from three shopping centres in the Zadar area (Croatian coast) to establish a list of most frequently bought fruit, vegetables, whole cereals, and legumes and to calculate dietary fibre (DF) intake for each of the top-selling items and conversion factors for each food group. We then used these conversion factors to calculate individual total fibre intake (TFI) in our population. It was...
    The aim of this cross-sectional study was to determine the associations between health dietary patterns, knowledge, and consumption of dietary fiber (DF) with frequency of food label reading on food products with special reference to DF.... more
    The aim of this cross-sectional study was to determine the associations between health dietary patterns, knowledge, and consumption of dietary fiber (DF) with frequency of food label reading on food products with special reference to DF. The study was conducted in 2536 Croatian adults using an original questionnaire. Multiple linear regression models were used to assess associations between food label reading habits and predictor variables. Our study confirms the association between habits regarding the reading of labels on food products, especially in relation to information about DF with the sociodemographic factors of respondents, dietary food patterns and DF consumption, as well as knowledge and sources of information about DF. Women, individuals with a university-level education, and those living in an urban environment had more frequent labels used. Food habits as well as eating outside of the home were positive predictors while eating fast food was a negative predictor of foo...
    Cilj ovoga rada bio je procijeniti usklađenost energijskog i nutritivnog sastava jelovnika u dječjim vrtićima Zadarske županije primjenom dviju metoda praćenja: analitičkim određivanjem kompletnih dnevnih obroka i izračunom na temelju... more
    Cilj ovoga rada bio je procijeniti usklađenost energijskog i nutritivnog sastava jelovnika u dječjim vrtićima Zadarske županije primjenom dviju metoda praćenja: analitičkim određivanjem kompletnih dnevnih obroka i izračunom na temelju normativa kompletnih obroka, te dobivene vrijednosti usporediti s važećim standardima. Na temelju provedenih mjerenja i izračuna, identificirati moguća kritična razdoblja odstupanja nutrijenata i energijskog unosa unutar godine, u odnosu na važeće standarde, te provesti razdoblja ponuđenih namirnica u sklopu jelovnika. Metode su uključivale sljedeće: prikupljanje kompletnih dnevnih obroka i normativa tijekom petnaest uzastopnih dana kroz tri godišnja doba; analitičko određivanje makronutrijenata u prikupljenim obrocima; izračun makronutrijenata iz normativa i tablica o kemijskom sastavu hrane i pića; izračun energijske vrijednosti iz analitički i računski određenih nutrijenata; usporedbe dobivenih vrijednosti s obzirom na godišnje doba i primijenjenu m...
    CILJ: Implementacija nastavnih sadržaja iz podrucja palijativne skrbi u studijski program sestrinstva na Odjelu za zdravstvene studije Sveucilista u Zadru stvorit ce preduvjete za organizaciju kvalitetne palijativne skrbi na podrucju... more
    CILJ: Implementacija nastavnih sadržaja iz podrucja palijativne skrbi u studijski program sestrinstva na Odjelu za zdravstvene studije Sveucilista u Zadru stvorit ce preduvjete za organizaciju kvalitetne palijativne skrbi na podrucju Zadarske županije. Primarni cilj rada je prikazati tijek osnivanja nastavne baze palijativne skrbi na Odjelu za zdravstvene studije Sveucilista u Zadru. METODE: U radu je shematski i slikovno prikazan tijek osnivanja nastavne baze i sadržaj nastavnih planova kolegija Gerijatrijska i palijativna skrb te Palijativna zdravstvena njega kao sastavnih dijelova nastavnog plana i programa preddiplomskog odnosno diplomskog studija sestrinstva. Povezanost strucne i sveucilisne razine obrazovanja odražava se prikazom razlikovnog modula Palijativna skrb. Slikovno i shematski prikazane su poveznice Sveucilista u Zadru s nastavnim bazama Opcom bolnicom Zadar i Psihijatrijskom bolnicom Ugljan uz osvrt na metode palijativne skrbi i specificnosti palijativnog pristupa s...
    The aim of this research is to describe quality control procedures, procedures for validation and measurement uncertainty (MU) determination as an important element of quality assurance in food microbiology laboratory for qualitative and... more
    The aim of this research is to describe quality control procedures, procedures for validation and measurement uncertainty (MU) determination as an important element of quality assurance in food microbiology laboratory for qualitative and quantitative type of analysis. Accreditation is conducted according to the standard ISO 17025:2007. General requirements for the competence of testing and calibration laboratories, which guarantees the compliance with standard operating procedures and the technical competence of the staff involved in the tests, recently are widely introduced in food microbiology laboratories in Croatia. In addition to quality manual introduction, and a lot of general documents, some of the most demanding procedures in routine microbiology laboratories are measurement uncertainty (MU) procedures and validation experiment design establishment. Those procedures are not standardized yet even at international level, and they require practical microbiological knowledge, a...
    The purpose of the study was to compare two methods for destruction of biological material for selenium (Se) analysis: wet digestion by conductive heating in programmed digestion block and digestion in microwave oven. In both methods... more
    The purpose of the study was to compare two methods for destruction of biological material for selenium (Se) analysis: wet digestion by conductive heating in programmed digestion block and digestion in microwave oven. In both methods samples were prepared in a closed system using nitric acid. Selenium was analysed by electrothermal atomic absorption spectrometry. The results have shown that both methods are convenient for complete mineralisation and are accurate in determining selenium in a variety of foodstuffs. Microwave digestion, however, has the advantage of speed and simplicity over the conventional heating procedure.
    Background: Dietary fibre (DF) has been recognised as having many positive health effects. Hence, the objective of this research was to evaluate the consuming habits relating to DF on people from different countries, as well as their... more
    Background: Dietary fibre (DF) has been recognised as having many positive health effects. Hence, the objective of this research was to evaluate the consuming habits relating to DF on people from different countries, as well as their knowledge about the fibre rich foods and their attitudes towards food labelling. Methods: A descriptive cross-sectional study was undertaken on a non-probabilistic sample of 6010 participants from 10 countries. Descriptive statistics and inferential tests were done using SPSS software considering a level of significance of 5%. Results: The results suggested that the ingestion of fibre rich foods like fruit, vegetables and cereals, was low and far from the recommended amounts. It was also concluded that most people did not pay the desired level of attention to food labelling and nutritional information. Furthermore, the level of knowledge about dietary fibre was generally not satisfactory. Conclusion: In view of these findings it becomes important to develop actions to better inform the population and to make them better aware of the importance of dietary fibre in their habitual diet.
    Accreditation according to the HR EN ISO/ IEC:17025:2007 is applicable to all organizations performing tests and/or calibrations. These include, first-, secondand third-party laboratories, and laboratories where testing and/or calibration... more
    Accreditation according to the HR EN ISO/ IEC:17025:2007 is applicable to all organizations performing tests and/or calibrations. These include, first-, secondand third-party laboratories, and laboratories where testing and/or calibration forms a part of inspection and product certification (HR EN ISO/ IEC:17025, 2007). Accreditation of laboratories according to HR EN ISO/IEC:17025:2007 or other internationally recognized schemes is an instrument which contributes to confidence in international trade, to consumer’s and official bodies confidence, and is of concern of all participants in food chain. Summary
    Production, processing, transporting, selling, and consumption of food are highly resource intensive. Therefore, if they are not well managed the consequences for the environment are far-reaching. This study aimed at investigating... more
    Production, processing, transporting, selling, and consumption of food are highly resource intensive. Therefore, if they are not well managed the consequences for the environment are far-reaching. This study aimed at investigating behaviors and attitudes of the Croatian population concerning the influence of environmental and political determinants of food choices, and the socio-demographic factors associated with pro-environmental behavior. Data analysis involved a non-probabilistic sample of 1534 adult participants from Croatia who responded to a validated questionnaire from November 2017 to March 2018. To test differences between sociodemographic groups, Welch’s t-test (two groups) and ANOVA (multiple groups) were used. The relationship between age and motivators of food choices was analyzed with Pearson’s r correlation coefficient. Participants reported a neutral rate of agreement with the items, with the exception of items related to food waste and food origin, for which they e...
    PurposeThis study explored Croatian nursing students' knowledge and attitudes regarding brominated flame retardants (BFRs) as indicators of their predisposition to educate future patients. The purpose of the study was to identify... more
    PurposeThis study explored Croatian nursing students' knowledge and attitudes regarding brominated flame retardants (BFRs) as indicators of their predisposition to educate future patients. The purpose of the study was to identify knowledge gaps and barriers and to propose possible remedies.Design/methodology/approachThe cross-sectional survey was conducted on a convenience sample of 114 nursing students at undergraduate and graduate levels from three Croatian universities during the winter semester in the academic year 2018–2019. Descriptive and inferential statistical  analyses were performed using STATISTICA 13 software.FindingsSlightly over half of the students (58.49%) were knowledgeable of BFR health effects and 45.28% showed knowledge about its presence in the environment. Only 33.02% of students identified prenatal exposure effects and 24.53% answered correctly about legislative actions. Participants expressed modest interest in the topic (M = 3.15, SD = 1.35). Although i...
    Introduction: Nurses play diverse roles in educating patients about a healthy diet and in promoting healthy eating habits, through their conversations with patients and families and through their work providing enteral and parenteral... more
    Introduction: Nurses play diverse roles in educating patients about a healthy diet and in promoting healthy eating habits, through their conversations with patients and families and through their work providing enteral and parenteral feeding to critically ill patients. Given the position of nurses as key providers of dietary guidance, and given the health benefits of dietary fibre, we wanted to assess the level of fibre-related knowledge among nurses in Croatia.Objectives: The primary objective of this cross-sectional s tudy was to compare knowledge between nurses and the general population from Croatia about the health importance of consuming dietary fibre.Methods: Knowledge from 369 nurses and 727 residents from the general population about fibre was assessed using a validated survey developed by the CI&DETS Polytechnic Institute, Viseu, Portugal. Differences between nurses and the general population were assessed for significance using the Mann-Whitney U test and the Kruskal-Wall...
    Purpose Because dietary fibre has been recognized as a major ally to the maintenance of a healthy body as well as to help against the development of some chronic diseases, this paper aims to study the level of knowledge of a relatively... more
    Purpose Because dietary fibre has been recognized as a major ally to the maintenance of a healthy body as well as to help against the development of some chronic diseases, this paper aims to study the level of knowledge of a relatively wide range of people about the health effects related to the ingestion of dietary fibre in appropriate dosages. Design/methodology/approach A descriptive cross-sectional study was undertaken on a non-probabilistic sample of 6,010 participants. The data were collected from 10 countries in three different continents (Europe, Africa and America) and measured the level of knowledge regarding different health benefits from dietary fibre. The questionnaires were applied by direct interview after verbal informed consent. Findings The results obtained considering the general level of knowledge revealed a considerable degree of information about the benefits of fibre (average score of 3.54 ± 0.5, on a scale from 1 to 5). There were significant differences betw...
    ABSTRACT Children are prone to the adverse effects of toxic metals, including cadmium, due to specific physiological needs during growth and development. We evaluated the level of cadmium in daily meals offered to children (N =1000, age 4... more
    ABSTRACT Children are prone to the adverse effects of toxic metals, including cadmium, due to specific physiological needs during growth and development. We evaluated the level of cadmium in daily meals offered to children (N =1000, age 4 to 7) who attended public nurseries in the coastal area, where they received 80 % of their daily dietary needs. The duplicate portion technique for sampling total food and beverage during 15 consecutive workdays over three seasons (during 2008) was used. Cadmium was analysed in representative samples by electrothermal atomic absorption spectrometry. The estimated levels of ingested cadmium in the meals (expressed as average daily cadmium concentrations) in winter, spring and autumn were: 2.40±1.02, 1.53±1.36, and 0.57±0.38 μg/kg. Based upon a comparison with the dietary exposure of an average child consumer to cadmium, which is between 2.56 and 3.46 μg/kg bw a week (according to EFSA, 2011), we concluded that cadmium levels in the meals can be considered safe.
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    The aim of this research is to describe quality control procedures, procedures for validation and measurement uncertainty (MU) determination as an important element of quality assur¬ance in food microbiology laboratory for qualitative and... more
    The aim of this research is to describe quality control procedures, procedures for validation and measurement uncertainty (MU) determination as an important element of quality assur¬ance in food microbiology laboratory for qualitative and quantitative type of analysis. Accredita¬tion is conducted according to the standard ISO 17025:2007. General requirements for the competence of testing and calibration laboratories, which guarantees the compliance with stan¬dard operating procedures and the technical competence of the staff involved in the tests, re¬cently are widely introduced in food microbiology laboratories in Croatia. In addition to quality manual introduction, and a lot of general documents, some of the most demanding procedures in routine microbiology laboratories are measurement uncertainty (MU) procedures and vali¬dation experiment design establishment. Those procedures are not standardized yet even at international level, and they require practical microbiological knowled...
    In this study we assessed 48 samples of virgin olive oil collected along the Croatian Adriatic coast for quality control, and for the presence of residues of seven organochlorine pesticides and 17 congeners of polychlorinated biphenyls... more
    In this study we assessed 48 samples of virgin olive oil collected along the Croatian Adriatic coast for quality control, and for the presence of residues of seven organochlorine pesticides and 17 congeners of polychlorinated biphenyls (PCBs). Organochlorine pesticide levels ranged between below the limit of determination and 3.7 ng/g of oil, while PCBs ranged between below the limit of determination and 1.8 ng/g of oil. A larger problem than the presence of organochlorine compounds was that the seven tested oils (out of 48) did not meet some quality standards.
    This study aimed at identifying a calcium compound which could serve as an effective and safe dietary supplement in suckling rats over the period of intense growth and development. The main objective was to assess the effect of additional... more
    This study aimed at identifying a calcium compound which could serve as an effective and safe dietary supplement in suckling rats over the period of intense growth and development. The main objective was to assess the effect of additional calcium intake on skeletal calcium in suckling pups. Suckling Wistar rats were fed using a pipettor with one of the following calcium salts from day 6 to 14 after the birth: gluconate, hydrogenphosphate, carbonate (each suspended in cow’s milk), or chloride (in demineralised water). Control rats received only cow’s milk. Calcium in the carcass (body without organs and skin) was analysed by atomic absorption spectrometry. The only effective dietary supplement that produced no risk for the suckling pups’ growth was calcium hydrogenphosphate in cow’s milk in the total amount of 340 mg. That dose increased the daily calcium intake 3 to 4 times compared to non-supplemented controls, increasing carcass calcium content by about 16 per cent. Other calcium ...
    The concentration of trace elements (Cd, Cu, Fe, Mn, and Zn) was measured in different species of mushrooms (Boletaceae) and correlated with corresponding elements in soil. Five different species of Boletaceae mushrooms and soil samples... more
    The concentration of trace elements (Cd, Cu, Fe, Mn, and Zn) was measured in different species of mushrooms (Boletaceae) and correlated with corresponding elements in soil. Five different species of Boletaceae mushrooms and soil samples were collected from forests of Varazdin county in Croatia. Trace elements were analyzed by atomic absorption spectrometry in mushrooms and in EDTA-extracted soil. The results showed that Cd, Cu, and Zn are concentrated in mushroom tissue from soil with transfer factors (mushroom/soil) of 27.0,10.5, and 12.5, respectively. Cadmium incorporated much less in Leccinum (mean 0.73 mg/kg dry weight) than in Boletus, Xerocomus, or Gyroporus (respective means, 6.8, 8.4, and 12.3 mg/kg). Copper and Zn were accumulated in all collected mushrooms (14.7-35.6 and 109-179 mg/kg, respectively) with no difference among species. There was no accumulation of Fe and Mn in mushrooms, but concentrations differed between species, with lowest values in Leccinum. Iron varied...
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    The aim of this study was to evaluate possible genotoxic damage of cadmium chloride exposure in suckling rats by means of the comet assay and the in vivo micronucleus test of rat blood lymphocytes, because no information is available on... more
    The aim of this study was to evaluate possible genotoxic damage of cadmium chloride exposure in suckling rats by means of the comet assay and the in vivo micronucleus test of rat blood lymphocytes, because no information is available on the genotoxic effect of cadmium in rats at this early age. Pups were receiving cadmium (as CdCl(2).H(2)O) orally in fractions of 0.5 mg for 9 days, totalling 4.5 mg Cd kg(-1) body wt, or were given a single subcutaneous injection of 0.5 mg Cd kg(-1) body wt. Some pups in both exposed groups were receiving calcium supplement (CaHPO(4).2H(2)O) in feed to reduce the body load of cadmium. Control pups did not receive either cadmium or calcium supplement. Cadmium in the carcass and organs was measured by atomic absorption spectrometry. The results showed that the cadmium body burden was significantly lower when the animals were receiving calcium supplements along with oral cadmium. The results of the micronucleus and comet assays showed significant differences between the control and exposed groups, regardless of the route of cadmium administration. The only statistically significant difference between the two exposed groups (oral cadmium and oral cadmium + calcium supplements) was in the number of micronuclei. The results of the comet assay showed that tail length differed statistically only between the control and all exposed groups, regardless of the route of cadmium administration. It can be concluded that the applied cadmium doses caused detectable genome damage but it was lower in calcium-treated pups receiving cadmium orally.
    ... Average daily intake of Seb (μg Se day–1) ... Johan Libbey Eurotext, Paris, p 389 4. Pelus E, Arnaud J, Ducros V, Faure H, Favier A, Roussel AM (1994) Int J Food Sci Nutr 45:63 5. Pfannhauser W, Wilglinger M, Sima A (1997) 6th Nordic... more
    ... Average daily intake of Seb (μg Se day–1) ... Johan Libbey Eurotext, Paris, p 389 4. Pelus E, Arnaud J, Ducros V, Faure H, Favier A, Roussel AM (1994) Int J Food Sci Nutr 45:63 5. Pfannhauser W, Wilglinger M, Sima A (1997) 6th Nordic Symposium on Trace Elements in Human ...
    ... Average daily intake of Seb (μg Se day–1) ... Johan Libbey Eurotext, Paris, p 389 4. Pelus E, Arnaud J, Ducros V, Faure H, Favier A, Roussel AM (1994) Int J Food Sci Nutr 45:63 5. Pfannhauser W, Wilglinger M, Sima A (1997) 6th Nordic... more
    ... Average daily intake of Seb (μg Se day–1) ... Johan Libbey Eurotext, Paris, p 389 4. Pelus E, Arnaud J, Ducros V, Faure H, Favier A, Roussel AM (1994) Int J Food Sci Nutr 45:63 5. Pfannhauser W, Wilglinger M, Sima A (1997) 6th Nordic Symposium on Trace Elements in Human ...
    Unlike fast and restaurant food, diet rich in fibre is known to contribute significantly to health. The aim of our study was to assess eating habits such as consumption of fibre-rich, fast, and restaurant food of the general population in... more
    Unlike fast and restaurant food, diet rich in fibre is known to contribute significantly to health. The aim of our study was to assess eating habits such as consumption of fibre-rich, fast, and restaurant food of the general population in Croatia. For this purpose we used a validated survey designed by the Polytechnic Institute Viseu in Portugal, which includes questions about demographics, good eating habits related to the consumption of the main sources of dietary fibre (fruit, vegetables, and whole grains), and unhealthy eating habits related to the consumption of fast food and restaurant meals. Between October 2014 and March 2015 we received answers from 2,536 respondents aged between 18-70 years, of whom 67.4 % were women and 32.6 % were men. Most respondents reported consuming one serving of vegetables and one piece of fruit a day, and whole grains every other day. Women and urban residents reported consuming larger amounts of fruit, vegetables, and whole grains than men (p&lt...
    From November 1977 to March 1978, forced expiratory volumes (FEVs) of 78 second graders living in a high sulfur dioxide and smoke pollution area were compared with FEVs of 70 second graders living in a clean air area. The incidence of... more
    From November 1977 to March 1978, forced expiratory volumes (FEVs) of 78 second graders living in a high sulfur dioxide and smoke pollution area were compared with FEVs of 70 second graders living in a clean air area. The incidence of acute respiratory diseases in these children and their families was also comparatively studied during the same period. Indoor and outdoor measurements of sulfur dioxide and smoke, as well as additional measurements of suspended particulate matter (SPM) and sulfate were conducted. The results of the study suggest that at the actual average annual exposure to sulfur dioxide, smoke, and SPM of 70-80 microgram/m3, 60-80 microgram/m3, and 130-200 microgram/m3, respectively, with frequent exposures to three to five times higher daily sulfur dioxide and smoke concentrations and two times higher daily concentrations of SPM during the heating season, certain effects on the ventilatory functions and occurrence of acute respiratory diseases can be expected.
    The objective of this work was to study the degree of knowledge about dietary fibre (DF), as influenced by factors such as gender, level of education, living environment or country. For this, a descriptive cross-sectional study was... more
    The objective of this work was to study the degree of knowledge about dietary fibre (DF), as influenced by factors such as gender, level of education, living environment or country. For this, a descriptive cross-sectional study was undertaken on a non-probabilistic sample of 6010 participants from 10 countries in different continents (Europe, Africa and America). The results showed that the participants revealed on average a positive but still low global level of knowledge, which alerts for the need to take some actions to further inform the population about DF and its role as a component of a healthy diet. The results also indicated differences between genders, levels of education, living environments and countries. The highest level of knowledge was revealed by the participants from female gender, with higher education and living in urban areas. Concerning the country, the best informed were the participants from Romania, followed by those from Portugal and Turkey while the least ...
    Following the idea of interrelated role of epidemiology and toxicology in risk assessment and dose response evaluation, this paper presents certain experiences from the authors' studies of health effects of environmental exposure... more
    Following the idea of interrelated role of epidemiology and toxicology in risk assessment and dose response evaluation, this paper presents certain experiences from the authors' studies of health effects of environmental exposure to lead and manganese. Epidemiologic observations of adverse effects on female reproductive integrity in a lead smeltery area agree with the experimental data. Animal studies show that the adverse reproductive effects in females are time and dose-related, and reversible after exposure has ceased. Field studies show that effects of manganese on the respiratory system in a polluted region are dose, age and season-related. Cytotoxic effects of manganese, including the inhibitory effect on alveolar macrophages described in in vitro studies on mammalian cells confirm the epidemiologic observations. Authors conclude that in the process of risk assessment, toxicology and epidemiology have to act together. Available human data should be combined with experimental findings and data on the mechanism of toxic action.
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