Research Interests:
Analysis of biochemical transformations occurring in the course of SIRS and sepsis inclines a critical attitude towards, obligatory nowadays, criteria of these states. They are non-specific and, in an unsatisfying way, they define the... more
Analysis of biochemical transformations occurring in the course of SIRS and sepsis inclines a critical attitude towards, obligatory nowadays, criteria of these states. They are non-specific and, in an unsatisfying way, they define the state of a current immunological response of the organism from the laboratory as well as clinical point of view. The purpose of the project of a new approach to sepsis--PIRO is to create a more objective, measurable system of evaluation of clinical state of a patient suspected of sepsis.
Research Interests:
Research Interests:
Urinary albumin excretion is an established risk factor for renal and cardiovascular events. Measurement of albumin in the urine daily collection is the gold standard in assessing albuminuria. The 24-hour urine collection is cumbersome... more
Urinary albumin excretion is an established risk factor for renal and cardiovascular events. Measurement of albumin in the urine daily collection is the gold standard in assessing albuminuria. The 24-hour urine collection is cumbersome procedure that generates a lot of mistakes, therefore other methods have been proposed. The aim of the study was to compare the assessment of albuminuria in the 24-hour urine collection and in the second morning urine sample as well as to determine UACR. The study included 32 patients, from whom the daily and the second morning urine samples were collected. In both samples, the albumin and creatinine concentrations were determined and the urinary albumin: creatinine ratio (UACR) was calculated. An excellent correlation between the UACR determined from the 24-hour urine collection and the other portion of the second morning sample was obtained within a wide range of albuminuria values (r = 0.9825). Furthermore, a better correlation between the same characteristics was obtained in urine of patients with normoalbuminuria when UACR did not exceed 30 mg/g creatinine (r = 0.9771). Above this value, the correlation was slightly lower for micro- and macroalbuminuria and equalled 0.9249 and 0.9332, respectively. On the basis of the obtained results it can be concluded that the second morning urine sample with the determination of UACR is a good alternative to the 24-hour urine collection and the first morning urine sample which are burdened with a preanalytical error.
Research Interests: Humans, Female, Male, Young Adult, Periodicity, and 5 moreAged, Middle Aged, Adult, Reproducibility of Results, and Albuminuria
It has been found during last years that vitamins A, C, E and carotenoids, besides previously recognized functions of preventing particular lipido- and avitaminosis, significantly participate in the protection of the human body against... more
It has been found during last years that vitamins A, C, E and carotenoids, besides previously recognized functions of preventing particular lipido- and avitaminosis, significantly participate in the protection of the human body against oxidation stress that is characterized by balance disturbance between speed of free radical creation and reactive oxygen forms with pace of their neutralization by enzymes and antioxidants. The antioxidative system creates special protective barrier which includes numerous chemical form with antiperoxidative proprieties and vitamins are the most important part of it. The aim of this work was the profile of occurrence and antioxidative action of the vitamins A, C and E.
Research Interests:
The aim of the study was to evaluate the range of chronic inflammation in patients after second or subsequent myocardial infarction, and to compare it with its range in patients after first MI. C-reactive protein measurement clinical... more
The aim of the study was to evaluate the range of chronic inflammation in patients after second or subsequent myocardial infarction, and to compare it with its range in patients after first MI. C-reactive protein measurement clinical value was examined by high sensitivity CRP method (hsCRP) and compared to clinical value of other heart attack risk factors: total cholesterol (TC) and cholesterol in HDL fraction (HDL-C), triglycerides (TG) and fibrinogen (FBG). The research included 120 patients in two investigated groups: Z2--30 patients undergoing secondary prevention after second (subsequent) heart attack, and Z1--30 patients with first myocardial infarction. There were two reference groups: KOR--30 people undergoing planned coronarography and K--30 healthy individuals. Groups Z2, Z1 and KOR patients were treated with PCI (percutaneous coronary intervention). HsCRP medians for group Z2 (2.65 mg/I) and groups Z, (2.25 mg/) and KOR (2.35 mg/l) were significantly higher than in group K (1.10 mg/dl). Highest clinical value of hsCRP in coronary attack prediction was confirmed. Relative area under ROC curve (AUC) was highest in group 2, higher than in group Z1 (0.778 and 0.711, respectively). In patients undergoing secondary prevention, group Z2, hsCRP measurement showed higher clinical value in subsequ ent myocardial infarction prediction than in first one. In groups Z2 and Z1, HDL-C measurement also shows high diagnostic value for coronary risk assessment. AUC are 0.875 and 0.782, respectively. However, in treatment with statins, high AUC values do not necessarily reflect myocardial infarction risk.
Research Interests:
The aim of the study was to estimate cardiovascular diseases risk based on the concentration of total blood cholesterol, HDL-cholesterol, triglycerides, glucose, C-reactive protein determined by high sensitivity assay and platelet count.... more
The aim of the study was to estimate cardiovascular diseases risk based on the concentration of total blood cholesterol, HDL-cholesterol, triglycerides, glucose, C-reactive protein determined by high sensitivity assay and platelet count. The study was carried out in a group of healthy professionally active policemen in the Lodz region. The significant factors of atherosclerosis are: life style including diet and physical activity. Policeman profession requires a greater physical activity and good physical condition. The study group consisted of 836 policemen aged 31-63 years, mean 43,5 +/- 4,9 years. The mean value of total cholesterol was 217,7 mg/dl (69% > 200 mg/dl), cholesterol HDL--53,6 mg/dl (8% < 40 mg/dl), TG--168,8 mg/dl (47% > 150 mg/dl), CRP--1,95 mg/l (median 1,30 mg/l), 19,3% > 3 mg/l, a 7,4% > 5 mg/l, glucose--92,6 mg/dl (median 91 mg/dl), 7,2% > or = 110 mg/dl, a 20% > or = 100 mg/dl. The platelet count was 245,8 (median 244 x 103/microl), 99% < 350 x 103/microl. The concentration of lipids parameters is similar both for population of Lodz region and another population. In respect to proper values of the total cholesterol and HDL-cholesterol observed for Polish population including Lodz, the number of people with abnormal values is lower. The contrary tendency concerns to triglycerides.
Research Interests:
The establishment of reference values comparative ranges for each laboratory test result does not meet expectations. THE AIM OF THE STUDY was to make a comparative analysis of the reference values (RV) used in medical diagnostic... more
The establishment of reference values comparative ranges for each laboratory test result does not meet expectations. THE AIM OF THE STUDY was to make a comparative analysis of the reference values (RV) used in medical diagnostic laboratories (MLD) in Poland for concentrations in serum or plasma biochemical parameters (potassium, calcium, creatinine, and glucose) in adults. The data source was the information sent to the Centre for Quality Assessment in Laboratory Medicine by MLD participating in programs interlaboratory surveys. Studies have shown that there is a great diversity of reference values for the analyzed biochemical parameters used in MLD. The study also demonstrated the use by MLD various units of measurement systems (SI and weight-volume). A large variation of the reference values in the parameters significantly hinders the interpretation of laboratory results by physician.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
In 90 workers of the petrochemical industry occupationally exposed to a mixture of petroleum derivatives, including 60 persons exposed mainly to phenol and 30 to toluene, the metabolic activity of the liver was examined using the... more
In 90 workers of the petrochemical industry occupationally exposed to a mixture of petroleum derivatives, including 60 persons exposed mainly to phenol and 30 to toluene, the metabolic activity of the liver was examined using the antipyrine test. In both groups of subjects, an increase of antipyrine clearance in saliva was observed. Occupational exposure to lipophylic derivatives of petroleum seems to induce the function of liver microsomal enzymes.
Research Interests: Petroleum, Humans, Liver, Male, Aged, and 7 moreMiddle Aged, Saliva, Phenols, Adult, Occupational Exposure, Toluene, and Antipyrine
Research Interests: Poland, Humans, Male, Phenol, Middle Aged, and 5 morePhenols, Adult, Chemical Industry, Benzene, and Air Pollutants
The authors suggest that identification of high-risk individuals, modification of nutritional habit, preventing the smoking habit, increased physical activity, low salt intake, and early diagnosis and treatment of hypertension should be... more
The authors suggest that identification of high-risk individuals, modification of nutritional habit, preventing the smoking habit, increased physical activity, low salt intake, and early diagnosis and treatment of hypertension should be the most important methods used for atherosclerosis and IHD prevention in youths.
Research Interests:
The influence of ethanol on benzene toxicity in the course of prolonged 26-week intoxication was investigated in Wistar rats. A dose of 1/30 DL50/24h of benzene in oil solution was administered subcutaneously once daily, 5 days a week.... more
The influence of ethanol on benzene toxicity in the course of prolonged 26-week intoxication was investigated in Wistar rats. A dose of 1/30 DL50/24h of benzene in oil solution was administered subcutaneously once daily, 5 days a week. Drinking water was replaced by a 10% water solution of ethanol ad libidum. Specimens of the liver, spleen, kidneys, lungs, and heart were taken for histopathological examination. Changes in the liver confirmed the hepatotoxic action of benzene and were unaffected by the simultaneous administration of ethanol. In the lungs and spleen the lymphatic system showed signs of damage which became more pronounced in joint exposure to benzene plus ethanol. The authors conclude that alcohol abuse by people professionally exposed to benzene and other lipophilic petroleum derivatives (as in the petrochemical industry) may increase the toxic effects of these substances.
Research Interests:
209 workers exposed to airborne benzene and phenol at the Mazovian Refining Petrochemical Works, at three different plants, i.e. of Phenol, Gasoline Composing and Water -- Wastes, have been examined. It has been demonstrated that urine... more
209 workers exposed to airborne benzene and phenol at the Mazovian Refining Petrochemical Works, at three different plants, i.e. of Phenol, Gasoline Composing and Water -- Wastes, have been examined. It has been demonstrated that urine phenol content in workers working in an open plant area is very often inadequate to the airborne time-weighted averaged concentration of benzene and phenol. It has been shown more advisable to determine urine phenol concentrations twice: before and after the work shift. At the same time some specific methods have been recommended for determining phenol concentrations in urine. These are: gas chromatographic method of Dirmikis and Darbre, modified in this laboratory, and Gibbs' colorimetric method modified by Bardodej with 4-amino-antipyrine or Gibbs' colorimetric method with 2,6-dibromoquinone-4-chloroimide modified by Hanke et al. The Theis-Benedict method should not be applied in medical analytical laboratory because of its low specificity to phenol.
Research Interests:
Forty petrochemical industry workers occupationally exposed to benzene levels within TLVs (30 mg.m-3) had their hepatic microsomal enzyme function quantitatively assessed by the antipyrine test. The clearance (C1AP) and half life (P1/2)... more
Forty petrochemical industry workers occupationally exposed to benzene levels within TLVs (30 mg.m-3) had their hepatic microsomal enzyme function quantitatively assessed by the antipyrine test. The clearance (C1AP) and half life (P1/2) of antipyrine in the saliva were determined. The benzene-exposed workers exhibited increased C1AP and decreased P1/2, as compared to 20 control subjects. The occupational exposure to benzene at the concentrations exceeding 30 mg.m-3 seems to induce the microsomal enzyme function of the liver.
Research Interests: Petroleum, Humans, Male, Middle Aged, Saliva, and 5 moreAdult, Chemical Industry, Biotransformation, Benzene, and Antipyrine
In comparison with healthy persons, chronic uremic patients on regular hemodialysis treatment had significantly higher blood serum concentrations of alpha 1-acid glycoprotein, ceruloplasmin and C4 complement component, while levels of... more
In comparison with healthy persons, chronic uremic patients on regular hemodialysis treatment had significantly higher blood serum concentrations of alpha 1-acid glycoprotein, ceruloplasmin and C4 complement component, while levels of haptoglobin, C3 and transferrin were lower. Serum alpha 2-macroglobulin and alpha 1-antitrypsin levels were similar in both groups. Hemodialysis with cuprophan membrane induced only slight changes in some of these glycoproteins during a 48-hour follow-up period. Seven hours after termination of hemodialysis slight, but significant, decreases in blood serum transferrin and alpha 1-antitrypsin concentrations were observed. Hemodialysis thus does not seem to induce a conspicuous acute-phase reaction.
Research Interests:
Research Interests:
Five male volunteers were exposed to benzene vapour (100 mg X m-3) during 2 h singly and during 4 successive days. The mean retention of benzene in the lungs and mean dose amounted to 61% and 74 mg, respectively. Two phases of the... more
Five male volunteers were exposed to benzene vapour (100 mg X m-3) during 2 h singly and during 4 successive days. The mean retention of benzene in the lungs and mean dose amounted to 61% and 74 mg, respectively. Two phases of the excretion of unchanged benzene in the expired air (t 1/2 approximately 2 h and approximately 20 h) and phenol in the urine (t 1/2 approximately 3 h and approximately 12 h) were observed. The observed half time values were similar after single and repeated exposure. Respective values for 4-days experiment amounted to 28 and 47% of the dose absorbed during the last day of exposure. Concentrations of benzene in the expired air and the rate of excretion of phenol in urine were higher after 4-days exposure than after single exposure, which results from benzene toxicokinetics. Coefficients of kinetic processes rate amounted to k2 = 0,15; k3 = 0,07; k4 = 0,06 and sigma kEM = 0,16 X h-1.
Research Interests:
The aim of the study was to evaluate the coagulant and fibrinolytic system, lipid economy and neurological condition in patients with various types of retinal vein occlusion. The material was 70 patients: 8 with diagnosed congestive... more
The aim of the study was to evaluate the coagulant and fibrinolytic system, lipid economy and neurological condition in patients with various types of retinal vein occlusion. The material was 70 patients: 8 with diagnosed congestive venous retinopathy, 27 with ischaemic central retinal vein occlusion and 35 with central retinal vein branch occlusion. Apart from the neurological evaluation, every patient underwent determination of: hematocrit, number of blood platelets, total plasma protein with its electrophoretic division, plasma fibrinogen level, kaolin-cephalin and prothrombin coefficient, and the level of triglycerides, total cholesterol with its fractions LDL and HDL. RESULTS: Considerable percentage of patients with arterial hypertension and suffering from partial or multi-symptomatic hemiparesis associated with complication in plasma lipid content indicates significant atheromatous changes in vascular wall which promote occlusion formation. The role of rheological factors in ...
BADANIA WYBRANYCH LABORATORYJNYCH CZYNNIKÓW RYZYKA CHORÓB SERCOWO-NACZYNIOWYCH U ZDROWYCH CZYNNYCH ZAWODOWO POLICJANTÓW WOJEWÓDZTWA ŁÓDZKIEGO 1 Zakład Diagnostyki Laboratoryjnej ZOZ MSWiA w Łodzi 2 Oddział Kardiologii z Pododdziałem Kardiologii Interwencyjnej ZOZ MSWiA w Łodzi 3 Poradnia Medycyny...more
Introduction: The role of erythropoietin (EPO) in patients with renal cysts remains unclear. Some authors suggest that EPO may be produced in renal cysts (RC). The aim of the study was to answer the question why concentration of EPO in... more
Introduction: The role of erythropoietin (EPO) in patients with renal cysts remains unclear. Some authors suggest that EPO may be produced in renal cysts (RC). The aim of the study was to answer the question why concentration of EPO in the cystic fluid (fEPO) is higher than the serum level of EPO. Material and methods: Serum levels of EPO (sEPO) and cystic fluid levels of EPO (fEPO) were determined in a group of 33 patients (age ranging from 24 to 56 years) with renal cysts and no symptoms of either renal insufficiency or anaemia. Similar tests (excluding fEPO) were performed in the healthy controls – K (33 adults, age ranging from 26 to 53 years). Haematological parameters and serum urea (UREA) and creatinine (CREA) concentration were also determined. Results: Levels of EPO in the cystic fluid were, on the average 35 times higher than serum levels of EPO. Levels of sEPO in patients with RC were comparable to the control group C. Significantly higher urea levels, lower reticulocyte ...
Background: The study evaluated association of new obesity indices – waist-to-hip ratio (WHtR), body adiposity index (BAI), visceral adiposity index (VAI) and classical obesity parameters: body mass index (BMI), waist circumference (WC)... more
Background: The study evaluated association of new obesity indices – waist-to-hip ratio (WHtR), body adiposity index (BAI), visceral adiposity index (VAI) and classical obesity parameters: body mass index (BMI), waist circumference (WC) and waist-to-hip ratio (WHR) – with high-sensitivity C-reactive protein (hs-CRP) as a cardiovascular risk factor in obese patients. Material/Methods: Sixty-six adult obese (BMI ≥30 kg/m2) non-diabetic out-clinic patients (F/M – 40/26), aged 55.8±11.6 years, were divided into 3 groups according to hs-CRP serum levels: A – ≤1 mg/L (n=11), B – 1 to 3 mg/L (n=27), and C – >3 mg/L (n=28). Additionally, we divided all the patients into 2 groups: treated with statins/fibrates (n=37) and untreated (n=29). Results: In group C, WHtR was significantly higher compared to group B (0.71±0.07 vs. 0.66±0.06; p<0.05) and group A (0.71±0.07 vs. 0.64±0.06; p<0.05); BMI was significantly higher than in group A (37.9±5.3 kg/m2 vs. 33.4±1.8 37.9±5.3 kg/m2; p<0...
ecent guidelines recommended by ESC, ACC, AHA, and WHF concerning biomarkers of myocardial necrosis also apply to the work of clinical laboratories. Methodological modification for tests used in determining cardiac biomarkers reduced the... more
ecent guidelines recommended by ESC, ACC, AHA, and WHF concerning biomarkers of myocardial necrosis also apply to the work of clinical laboratories. Methodological modification for tests used in determining cardiac biomarkers reduced the time of the analytical procedure to 9 min (STAT version of the tests). We decided to determine and compare analytical quality of the tests in standard and STAT versions for determining serum level: troponin T, MB isoenzyme of creatine kinase, and myoglobin, as well as to verify whether the TnThs STAT test meets the following requirements: CV<10% at the level close to diagnostic, equal to the 99th percentile of reference population, and turnaround time<60 min. We evaluated real precision and accuracy for both standard and STAT versions of tests as well as the correlation of results of physiological and pathological levels. Additionally, observations of turnaround time were made. Calculated values of total errors did not exceed the recommended a...
Research Interests:
The establishment of reference values comparative ranges for each laboratory test result does not meet expectations. THE AIM OF THE STUDY was to make a comparative analysis of the reference values (RV) used in medical diagnostic... more
The establishment of reference values comparative ranges for each laboratory test result does not meet expectations. THE AIM OF THE STUDY was to make a comparative analysis of the reference values (RV) used in medical diagnostic laboratories (MLD) in Poland for concentrations in serum or plasma biochemical parameters (potassium, calcium, creatinine, and glucose) in adults. The data source was the information sent to the Centre for Quality Assessment in Laboratory Medicine by MLD participating in programs interlaboratory surveys. Studies have shown that there is a great diversity of reference values for the analyzed biochemical parameters used in MLD. The study also demonstrated the use by MLD various units of measurement systems (SI and weight-volume). A large variation of the reference values in the parameters significantly hinders the interpretation of laboratory results by physician.
Research Interests:
Urinary albumin excretion is an established risk factor for renal and cardiovascular events. Measurement of albumin in the urine daily collection is the gold standard in assessing albuminuria. The 24-hour urine collection is cumbersome... more
Urinary albumin excretion is an established risk factor for renal and cardiovascular events. Measurement of albumin in the urine daily collection is the gold standard in assessing albuminuria. The 24-hour urine collection is cumbersome procedure that generates a lot of mistakes, therefore other methods have been proposed. The aim of the study was to compare the assessment of albuminuria in the 24-hour urine collection and in the second morning urine sample as well as to determine UACR. The study included 32 patients, from whom the daily and the second morning urine samples were collected. In both samples, the albumin and creatinine concentrations were determined and the urinary albumin: creatinine ratio (UACR) was calculated. An excellent correlation between the UACR determined from the 24-hour urine collection and the other portion of the second morning sample was obtained within a wide range of albuminuria values (r = 0.9825). Furthermore, a better correlation between the same cha...
Research Interests: Humans, Female, Male, Young Adult, Periodicity, and 5 moreAged, Middle Aged, Adult, Reproducibility of Results, and Albuminuria
It has been found during last years that vitamins A, C, E and carotenoids, besides previously recognized functions of preventing particular lipido- and avitaminosis, significantly participate in the protection of the human body against... more
It has been found during last years that vitamins A, C, E and carotenoids, besides previously recognized functions of preventing particular lipido- and avitaminosis, significantly participate in the protection of the human body against oxidation stress that is characterized by balance disturbance between speed of free radical creation and reactive oxygen forms with pace of their neutralization by enzymes and antioxidants. The antioxidative system creates special protective barrier which includes numerous chemical form with antiperoxidative proprieties and vitamins are the most important part of it. The aim of this work was the profile of occurrence and antioxidative action of the vitamins A, C and E.
Research Interests:
Significant progress in diagnosis and treatment of heart attack led European Society of Cardiology (ESC) and American College of Cardiology (ACC) to develop in 2007 a document on contemporary criteria for diagnosis of fresh myocardial... more
Significant progress in diagnosis and treatment of heart attack led European Society of Cardiology (ESC) and American College of Cardiology (ACC) to develop in 2007 a document on contemporary criteria for diagnosis of fresh myocardial infarction. In the case of percutaneous coronary intervention (PCI) in patients with initially normal serum concentrations of biomarkers troponin increase above the 99th percentile upper limit of the reference points to the occurrence of myocardial necrosis dislodgement. Conventionally assumed that the increased levels of biomarkers of value in excess of 3 x 99th percentile upper reference requires identification of MI in conjunction with PCI. To evaluate the dynamic of troponin concentrations and C-reactive protein in patients with the second or subsequent myocardial infarction undergoing percutaneous coronary intervention (PCI) and comparing the results obtained with the results obtained in patients with first myocardial infarction, in patients under...
Research Interests:
The effect of reactive oxygen species (ROS) on pathophysiology of many diseases is widely documented in numerous scientific publications. Nephropathies belong to pathologies in which ROS undoubtedly play a role. Imbalance between ROS and... more
The effect of reactive oxygen species (ROS) on pathophysiology of many diseases is widely documented in numerous scientific publications. Nephropathies belong to pathologies in which ROS undoubtedly play a role. Imbalance between ROS and antioxidants results in destructive effect on mesangial cells by altering lipid metabolism. This phenomenon is frequently observed in patients with glomerulonephritis and nephritic syndrome. Accumulation of hypoxanthine and oxygen in the mechanism of reperfusion damages renal cells by the produced ROS in patients with acute renal failure. Inactivation of nitric oxide by superoxide anion radical increases vascular resistance in renal arteries and contributes to the development of hypertensive nephropathy. Diabetic nephropathy, in which a significant role is ascribed to glycation end products, oxidative stress and decrease of antioxidant mechanisms, is another nephropathy associated with oxidant/antioxidant imbalance. Pro-inflammatory processes with a...
Research Interests:
The aim of the study was to estimate cardiovascular diseases risk based on the concentration of total blood cholesterol, HDL-cholesterol, triglycerides, glucose, C-reactive protein determined by high sensitivity assay and platelet count.... more
The aim of the study was to estimate cardiovascular diseases risk based on the concentration of total blood cholesterol, HDL-cholesterol, triglycerides, glucose, C-reactive protein determined by high sensitivity assay and platelet count. The study was carried out in a group of healthy professionally active policemen in the Lodz region. The significant factors of atherosclerosis are: life style including diet and physical activity. Policeman profession requires a greater physical activity and good physical condition. The study group consisted of 836 policemen aged 31-63 years, mean 43,5 +/- 4,9 years. The mean value of total cholesterol was 217,7 mg/dl (69% > 200 mg/dl), cholesterol HDL--53,6 mg/dl (8% < 40 mg/dl), TG--168,8 mg/dl (47% > 150 mg/dl), CRP--1,95 mg/l (median 1,30 mg/l), 19,3% > 3 mg/l, a 7,4% > 5 mg/l, glucose--92,6 mg/dl (median 91 mg/dl), 7,2% > or = 110 mg/dl, a 20% > or = 100 mg/dl. The platelet count was 245,8 (median 244 x 103/microl), 99% <...
Research Interests:
At present, the only reasonable action which reduces the case fatality rate at patients with severe sepsis and septic shock is the early diagnosis of infection which allows to introduce effective therapy. Although sepsis is a typical... more
At present, the only reasonable action which reduces the case fatality rate at patients with severe sepsis and septic shock is the early diagnosis of infection which allows to introduce effective therapy. Although sepsis is a typical clinical syndrome recognized in connection with infection, it should be remembered that infection does not reveal itself in each case. Also bacteriological blood culture does not confirm it in most cases. In view of few characteristic, especially in the initial phase of sepsis, clinical symptoms, laboratory investigations describing the present state of immune response of organism find a huge application. In our work we made an attempt to bring closer the latest markers of inflammatory reaction and infection such as acute phase proteins procalcytonin (PCT), proANP and lipopolisacharyde binding protein (LBP).
Research Interests:
Chronic hemodialysis in patients with renal insufficiency is a factor significantly intensifying oxidative stress, resulting in increased lipid and lipoprotein peroxidation. It intensifies atherosclerotic activity. Malonyldialdehyde (MDA)... more
Chronic hemodialysis in patients with renal insufficiency is a factor significantly intensifying oxidative stress, resulting in increased lipid and lipoprotein peroxidation. It intensifies atherosclerotic activity. Malonyldialdehyde (MDA) concentration in serum, and isolated fraction of low density lipoprotein is indicators of lipid peroxidation intensification and therefore arteriosclerosis development. Assessment of MDA concentration as a product of lipid peroxidation and lipid metabolism in patients on chronic dialysis. The study included 48 dialyzed patients, age 22-68 undergoing chronic dialysis for, on average, 56 months. On account of dialysis period the patients were included into 3 subgroups: group 1 (n = 17) dialysis period 2 years, group 2 (n = 13) dialysis period 2-5 years and group 3 (n = 18) dialysis period longer than 5 years. Control group consisted of 30 healthy subjects. We assessed: malonyldialdehyde in serum and isolated fraction of low density lipids (LDL) and h...
Research Interests:
Analysis of biochemical transformations occurring in the course of SIRS and sepsis inclines a critical attitude towards, obligatory nowadays, criteria of these states. They are non-specific and, in an unsatisfying way, they define the... more
Analysis of biochemical transformations occurring in the course of SIRS and sepsis inclines a critical attitude towards, obligatory nowadays, criteria of these states. They are non-specific and, in an unsatisfying way, they define the state of a current immunological response of the organism from the laboratory as well as clinical point of view. The purpose of the project of a new approach to sepsis--PIRO is to create a more objective, measurable system of evaluation of clinical state of a patient suspected of sepsis.
Research Interests:
The aim of the study was to evaluate dynamics of selected acute phase proteins in serum and cerebrospinal fluid (CSF) in children with viral meningitis and to assess diagnostic power of protein determination for detection and treatment... more
The aim of the study was to evaluate dynamics of selected acute phase proteins in serum and cerebrospinal fluid (CSF) in children with viral meningitis and to assess diagnostic power of protein determination for detection and treatment monitoring. 51 children with viral meningitis caused by ECHO 30 virus were included in the study group. Concentration of C-reactive protein (CRP), alpha 1-antitrypsin (AAT), alpha1-acid glycoprotein (AAG), alpha2-haptoglobin (HPT) and C3 complement fragment were determined in serum and CSF at entry and at day 14 after admittance to hospital. Control group for serum determination consisted in 30 healthy children (Group K1) and control group for CSF determination consisted in 19 hospitalized children in whom the diagnosis of meningitis was not confirmed (group K2). The greatest rise of acute phase proteins concentration was observed in children in case of HPT, AAG and C3 complement when determined in serum. Meningitis in children that was caused by ECHO...
Research Interests:
We examined whether an acute phase reaction could occur in children with lymphocytic meningitis of homogeneous etiology (parotitis epidemic from the Paramyxoviridae family), a sign of which would be an increase in concentrations of acute... more
We examined whether an acute phase reaction could occur in children with lymphocytic meningitis of homogeneous etiology (parotitis epidemic from the Paramyxoviridae family), a sign of which would be an increase in concentrations of acute phase proteins (APP's) in cerebrospinal fluid (CSF) and/or in blood serum. We also tested the usefulness of the determination of selected APP's concentrations in CSF and serum in diagnosis and monitoring of the course of the disease, provided that an increase in concentrations of selected APP's were discernible. Cases were 78 children with lymphocytic meningitis as a complication of parotitis epidemic. Controls were 30 healthy children (control group K1) and 19 children hospitalized with suspected meningitis (control group K2). The following APP's presence in CSF and serum were tested: C-reactive protein (CRP), alpha-2-haptoglobin (HPT), alpha-1-antitripsin (AAT) and alpha-1-acid glycoprotein (AAG), alpha-2-ceruloplasmin (CER) and al...
Research Interests:
Anterior segment ischaemia manifesting in aqueous malformation may follow tenotomy of the extraocular muscles. Tenotomy of 2 or 4 rectus eye muscles was performed on the right eyes of 23 adult rabbits. The aqueous humor was withdrawn from... more
Anterior segment ischaemia manifesting in aqueous malformation may follow tenotomy of the extraocular muscles. Tenotomy of 2 or 4 rectus eye muscles was performed on the right eyes of 23 adult rabbits. The aqueous humor was withdrawn from the anterior chamber of both eyes in 7 rabbits on the 11th, on the 22nd in 8 and on the 32nd postoperative day in 8 rabbits. Aqueous natrium, potassium and glucose concentrations and protein electrophoresis were examined. The only significant change was lower mean aqueous potassium concentration in the operated eyes as compared to controls on the 11th postoperative day. Tenotomy of rectus eye muscles in healthy rabbits evoked a discrete blood-aqueous barrier break-down.
Research Interests:
The aim of our paper was to observe the physical and chemical changes in the aqueous humor, lens, gelatous and fluid part of the vitreous body after the sulfurhexafluoride injection into the vitreous body. The New Zealand rabbits were... more
The aim of our paper was to observe the physical and chemical changes in the aqueous humor, lens, gelatous and fluid part of the vitreous body after the sulfurhexafluoride injection into the vitreous body. The New Zealand rabbits were randomized into the control and experimental group which were injected the gas into the vitreous body. The interesting samples were investigated during the 2nd, 7th and 14th day of the experiment. We estimated the volume of the fluid part of the vitreous body, its electrical resistance, the whole protein concentration (in the aqueous humor, lens and vitreous), and electrolite concentration (in the aqueous humor and fluid part of the vitreous body). We observed the increase of the fluid fraction in the vitreous body with the increasing of the whole protein concentration, changes in the K+ concentration and decrease of the electrical resistance in the vitreous fluid fraction. On the basis of our experimental results we can assume that expanding sulfurhex...
Research Interests:
The aim of this work was the comparison of selected immunological parameters in patients with chronic purulent and hypertrophic otorhinolaryngological diseases who were to be operated on. The patients were divided into two groups: 1-31... more
The aim of this work was the comparison of selected immunological parameters in patients with chronic purulent and hypertrophic otorhinolaryngological diseases who were to be operated on. The patients were divided into two groups: 1-31 patients aged 17-58 with chronic purulent otorhinolaryngological diseases. The following immunological parameters were carried out: rheumatoid factor (RF), immunoglobulins IgG, IgM, IgA, proteinogram including the level of albumin, globulin alpha1, alpha2, beta and gamma. In chronic purulent otorhinolaryngological diseases the characteristic features are the reduction of the level of globulin alpha1 (67.7%) and gamma (35.5%), while in hypertrophic cases globulin alpha1, and beta--91.7% each.
Research Interests:
The aim of the experimental studies, carried-out on 32 rabbits, was to explain the hypotensive effect of cytostatic drugs. It was found that a statistically significant lowering of the intraocular pressure without any changes in the... more
The aim of the experimental studies, carried-out on 32 rabbits, was to explain the hypotensive effect of cytostatic drugs. It was found that a statistically significant lowering of the intraocular pressure without any changes in the coefficient of the outflow of intraocular fluid occurred on the 1st, 2nd, 3rd and 4th day after administration of cytostatic drugs. On the 2nd and 3rd day, there were also observed a decrease of sodium and an increase of potassium blood concentrations. It ought to be supposed that those changes caused disturbances in Na/K ATP-ase activity with an inhibition of the active transport in the ciliary epithelium and a decrease of the secretion of intraocular fluid in ciliary body.
Research Interests:
40 adult patients were examined: 24 with purulent meningitis and 16 with lymphocytic meningitis. The control group consisted of 100 healthy people. In purulent meningitis patients in the 1st, 3rd, 5th, 7th, 14th and 28th day of the... more
40 adult patients were examined: 24 with purulent meningitis and 16 with lymphocytic meningitis. The control group consisted of 100 healthy people. In purulent meningitis patients in the 1st, 3rd, 5th, 7th, 14th and 28th day of the disease, concentrations of the following acute phase proteins were measured in serum: C-reactive protein, alpha 1-antitripsin, alpha 1-orosomucoid, alpha 2-ceruloplasmin, alpha 2-macroglobulin and alpha 2-haptoglobin. In lymphocytic meningitis patients concentrations of the above mentioned acute phase proteins were measured only in the 1st day of the disease. Usefulness of establishing alpha 2-haptoglobin, alpha 1-antitripsin, alpha 2-ceruloplasmin and particularly C-reactive protein concentrations for differential diagnosis of purulent and lymphatic meningitis was proved. Evaluation of C-reactive protein and alpha 1-antitripsin concentration kinetics proved to be fully useful for monitoring of seriousness of the course of purulent meningitis, and togethe...
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The aim of this study was to evaluate immunological, hematological and biochemical parameters in subjects chronically exposed to inhaled formaldehyde (F), phenol (Ph) and isomers of organic chlorohydrocarbons (Chc) released from Ksylamit.... more
The aim of this study was to evaluate immunological, hematological and biochemical parameters in subjects chronically exposed to inhaled formaldehyde (F), phenol (Ph) and isomers of organic chlorohydrocarbons (Chc) released from Ksylamit. Twenty-two office workers had been exposed for 6 months to vapors of Ksylamit, used for protection of felt plates inside the office building. The concentration of Ksylamit vapor was measured at the end of the 6-month period and the level of Ph and F in the air inside the building was 1.3 mg/m3 and 0.8 mg/m3, respectively. Most of the workers had ailments due to the irritant effect of inhaled Ksylamit, but no remarkable increase in morbidity was found during the 6 months of exposure and in a 3-year follow-up. Morphological parameters of blood and the number of natural killer (NK) cells in the group of exposed subjects were within the range observed in healthy subjects matched for age and sex. The number of T-lymphocytes and NK cell cytotoxicity were...
Research Interests: Immunology, Humans, Natural Killer cells, Female, Male, and 7 morePhenol, Middle Aged, Immune system, Phenols, Adult, Formaldehyde, and Blood cells
The mean platelet volume, measured optically and by the electrical impedance method, was analysed in the patients with chronic renal failure. All parameters describing the circulating platelet biomass except the platelet volume... more
The mean platelet volume, measured optically and by the electrical impedance method, was analysed in the patients with chronic renal failure. All parameters describing the circulating platelet biomass except the platelet volume heterogeneity were decreased in these patients. There is a strong inverse relation between the Ivy bleeding time and the platelet mass in the patient group. Our results indicate that the smaller platelets circulating in the patients with chronic renal failure may contribute to the uremic bleeding diathesis, and disturbed thrombocytopoiesis occurs in chronic renal failure.
Research Interests:
Research Interests:
Galectin-3, a biomarker associated with fibrosis and inflammation, has been implicated in development and progression of heart failure (HF) and predicts increased mortality and morbidity in this condition. HF frequently develops after... more
Galectin-3, a biomarker associated with fibrosis and inflammation, has been implicated in development and progression of heart failure (HF) and predicts increased mortality and morbidity in this condition. HF frequently develops after myocardial infarction (MI), contributing to worse outcome. The aim of this study is to assess the association between galectin-3 levels and various clinical parameters in acute phase of first MI treated with primary percutaneous coronary intervention (pPCI) in patients without prior HF. We included 145 consecutive patients with first acute MI treated with pPCI with stent implantation. Exclusion criteria were: prior HF, severe valvular diseases, coexisting cancers, connective tissue diseases and cirrhosis. Serum galectin-3 concentration was measured within 3-5 days after onset of acute MI. Thirty-six patients with the highest galectin-3 levels (4th quartile, &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 16 ng/mL) were compared to 109 subjects with a biomarker concentration ≤ 16 ng/mL. Elevated galectin-3 levels were more often observed in females, the elderly, subjects with coexisting diabetes, renaldysfunction and permanent atrial fi brillation (AF). Galectin-3 correlated with N-terminal pro-B-type natriuretic peptide (r = 0.27, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001) and high-sensitivity C-reactive protein (r = 0.20, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Multivariate analysis revealed that only new-onset AF and diuretics treatment during hospitalization were independently associated with galectin-3 levels &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 16 ng/mL. Elevated galectin-3 levels were associated with a higher rate of new-onset AF and diuretics treatment during hospitalization in patients with first MI treated with pPCI without prior HF.