SUMMARY - As a result of the application of tools from both molecular genetics and cytogenetics, ... more SUMMARY - As a result of the application of tools from both molecular genetics and cytogenetics, wheat-alien gene introgression projects carried out by means of chromosome engineering approaches are now benefiting by a substantial gain in efficiency with respect to the past. We have recently exploited such tools in producing durum wheat recombinant lines carrying separately the Lr19+Yp genes from
RFLP variation revealed by protein disulfide isomerase (PDI) coding gene sequences was assessed i... more RFLP variation revealed by protein disulfide isomerase (PDI) coding gene sequences was assessed in 170 accessions belonging to 23 species of Triticum and Aegilops. PDI restriction fragments were highly conserved within each species and confirmed that plant PDI is encoded either by single-copy sequences or by small gene families. The wheat PDI probe hybridized to single EcoRI or HindIII fragments
Three different probes, obtained by PCR amplification and labelling of different segments of a PD... more Three different probes, obtained by PCR amplification and labelling of different segments of a PDI cDNA clone from common wheat, were used to identify and assign to wheat chromosomes the gene sequences coding for protein disulphide isomerase (PDI). One of these probes, containing the whole coding region except for a short segment coding for the C-terminal sequence, displayed defined and specific RFLP patterns. PDI gene sequences were consequently assigned to wheat chromosome arms 4BS, 4DS, 4AL and 1BS by Southern hybridisation of EcoRI- HindIII- and BamHI-digested total DNA of nulli-tetrasomic and di-telosomic lines of Chinese Spring. This probe was also employed for assessing the restriction fragment length polymorphism in several hexaploid and tetraploid cultivated wheats. These showed considerable conservation at PDI loci; in fact polymorphism was only observed for the chromosome 1B fragment.
CSIRO Plant Industry, PO Box 1600, Canberra ACT 2601, Australia L. Tamas Plant Science CRC, PO Bo... more CSIRO Plant Industry, PO Box 1600, Canberra ACT 2601, Australia L. Tamas Plant Science CRC, PO Box 1600, Canberra ACT 2601, Australia Present address: DABAC, University of Tuscia, 01100 Viterbo, Italy ... M. Ciaffi · YK Lee · L. Tamas · R. Gupta J. Skerritt · R. Appels
Variation in high molecular weight (HMW) glutenin subunit composition among wild and cultivated e... more Variation in high molecular weight (HMW) glutenin subunit composition among wild and cultivated einkorn wheats (2n = 2x = 14, AA) was investigated using one- (SDS-PAGE and urea/SDS-PAGE) and two-dimensional (IEF × SDS-PAGE) electrophoretic analyses. The material comprised 150 accessions ofTriticum urartu, 160 accessions ofT. boeoticum, 24 accessions ofT. boeoticum subsp.thaoudar and 74 accessions of primitive domesticatedT. monococcum from many different germplasm collections. The biochemical characteristics of HMW-glutenin subunits ofT. boeoticum andT. monococcum were highly similar to one another but distinctly different from those ofT. urartu. All the species analysed were characterised by large intraspecific variation and only three HMW-glutenin subunit patterns were identical betweenT. boeoticum andT. monococcum. Consistent with the distinct nature ofT. urartu, all its HMW-glutenin patterns were different from those found inT. boeoticum andT. monococcum. The differences detected between these species might reflect their reproductive isolation and are consistent with recent nomenclatural and biosystematic treatments that recogniseT. urartu as separate species fromT. boeoticum andT. monococcum. The presence of three distinct glutenin components in some accessions of the species studied seems to be evidence for the existence of at least three active genes controlling the synthesis of the HMW-glutenin subunits in the A genome of wild and primitive domesticated diploid wheats. Results indicate also that HMW-glutenin subunits could represent useful markers for the evaluation of genetic variability present in different wild diploid wheat collections and subsequently for their conservation and future utilisation.
Abstract The deduced amino-acid sequences of 17 protein disulphide isomerase (PDI) and PDI-like c... more Abstract The deduced amino-acid sequences of 17 protein disulphide isomerase (PDI) and PDI-like cDNAs of wheat assigned to nine homoeologous groups were searched for conserved motives by comparison with characterized sequences in different protein databases. The wheat protein sequences encoded by genes of different homoelogous groups showed a high level of structural similarity with the corresponding protein sequences of other species clustering into the same phylogenetic group. The proteins of five groups (I ...
... SDS-PAGE). Variation of storage proteins at the Glu-A1, Glu-B1, Glu-B3 andGli-B1 loci was stu... more ... SDS-PAGE). Variation of storage proteins at the Glu-A1, Glu-B1, Glu-B3 andGli-B1 loci was studied. Electrophoretic analyses ... cultivated wheats. Recombination between the Glu-B3 and Gli-B1 loci was observed. To determine ...
ABSTRACT A collection of 180 Triticum durum lines from Turkey and 22 Italian cultivars were inves... more ABSTRACT A collection of 180 Triticum durum lines from Turkey and 22 Italian cultivars were investigated by electrophoretic techniques and evaluated for grain parameters such as protein content and sodium dodecyl sulphate (SDS)-sedimentation test. A total of 14 different patterns were observed for high-molecular-weight (HMW) glutenin subunits resulting from the combination of three alleles from the A genome and seven from the B genome. Three new allelic variants, one at Glu-A1 and two at Glu-B1, were found. Five different alleles were detected at the Gli-B1 locus. Genotypes with the same allelic variant at Gli-B1 showed inconsistency of composition in B group of low-molecular-weight (LMW) glutenin subunits. A total of 11 different B-subunit patterns were identified in the material analysed. A substantial proportion (78%) of the variation in gluten properties could be explained in terms of protein composition, with the LMW glutenin subunits making the largest contribution (54%). Results indicate that, in addition to Glu-B3 encoded proteins, variation at other loci coding for some intermediate and fastest B subunits might also influence rheological properties.
SUMMARY - As a result of the application of tools from both molecular genetics and cytogenetics, ... more SUMMARY - As a result of the application of tools from both molecular genetics and cytogenetics, wheat-alien gene introgression projects carried out by means of chromosome engineering approaches are now benefiting by a substantial gain in efficiency with respect to the past. We have recently exploited such tools in producing durum wheat recombinant lines carrying separately the Lr19+Yp genes from
RFLP variation revealed by protein disulfide isomerase (PDI) coding gene sequences was assessed i... more RFLP variation revealed by protein disulfide isomerase (PDI) coding gene sequences was assessed in 170 accessions belonging to 23 species of Triticum and Aegilops. PDI restriction fragments were highly conserved within each species and confirmed that plant PDI is encoded either by single-copy sequences or by small gene families. The wheat PDI probe hybridized to single EcoRI or HindIII fragments
Three different probes, obtained by PCR amplification and labelling of different segments of a PD... more Three different probes, obtained by PCR amplification and labelling of different segments of a PDI cDNA clone from common wheat, were used to identify and assign to wheat chromosomes the gene sequences coding for protein disulphide isomerase (PDI). One of these probes, containing the whole coding region except for a short segment coding for the C-terminal sequence, displayed defined and specific RFLP patterns. PDI gene sequences were consequently assigned to wheat chromosome arms 4BS, 4DS, 4AL and 1BS by Southern hybridisation of EcoRI- HindIII- and BamHI-digested total DNA of nulli-tetrasomic and di-telosomic lines of Chinese Spring. This probe was also employed for assessing the restriction fragment length polymorphism in several hexaploid and tetraploid cultivated wheats. These showed considerable conservation at PDI loci; in fact polymorphism was only observed for the chromosome 1B fragment.
CSIRO Plant Industry, PO Box 1600, Canberra ACT 2601, Australia L. Tamas Plant Science CRC, PO Bo... more CSIRO Plant Industry, PO Box 1600, Canberra ACT 2601, Australia L. Tamas Plant Science CRC, PO Box 1600, Canberra ACT 2601, Australia Present address: DABAC, University of Tuscia, 01100 Viterbo, Italy ... M. Ciaffi · YK Lee · L. Tamas · R. Gupta J. Skerritt · R. Appels
Variation in high molecular weight (HMW) glutenin subunit composition among wild and cultivated e... more Variation in high molecular weight (HMW) glutenin subunit composition among wild and cultivated einkorn wheats (2n = 2x = 14, AA) was investigated using one- (SDS-PAGE and urea/SDS-PAGE) and two-dimensional (IEF × SDS-PAGE) electrophoretic analyses. The material comprised 150 accessions ofTriticum urartu, 160 accessions ofT. boeoticum, 24 accessions ofT. boeoticum subsp.thaoudar and 74 accessions of primitive domesticatedT. monococcum from many different germplasm collections. The biochemical characteristics of HMW-glutenin subunits ofT. boeoticum andT. monococcum were highly similar to one another but distinctly different from those ofT. urartu. All the species analysed were characterised by large intraspecific variation and only three HMW-glutenin subunit patterns were identical betweenT. boeoticum andT. monococcum. Consistent with the distinct nature ofT. urartu, all its HMW-glutenin patterns were different from those found inT. boeoticum andT. monococcum. The differences detected between these species might reflect their reproductive isolation and are consistent with recent nomenclatural and biosystematic treatments that recogniseT. urartu as separate species fromT. boeoticum andT. monococcum. The presence of three distinct glutenin components in some accessions of the species studied seems to be evidence for the existence of at least three active genes controlling the synthesis of the HMW-glutenin subunits in the A genome of wild and primitive domesticated diploid wheats. Results indicate also that HMW-glutenin subunits could represent useful markers for the evaluation of genetic variability present in different wild diploid wheat collections and subsequently for their conservation and future utilisation.
Abstract The deduced amino-acid sequences of 17 protein disulphide isomerase (PDI) and PDI-like c... more Abstract The deduced amino-acid sequences of 17 protein disulphide isomerase (PDI) and PDI-like cDNAs of wheat assigned to nine homoeologous groups were searched for conserved motives by comparison with characterized sequences in different protein databases. The wheat protein sequences encoded by genes of different homoelogous groups showed a high level of structural similarity with the corresponding protein sequences of other species clustering into the same phylogenetic group. The proteins of five groups (I ...
... SDS-PAGE). Variation of storage proteins at the Glu-A1, Glu-B1, Glu-B3 andGli-B1 loci was stu... more ... SDS-PAGE). Variation of storage proteins at the Glu-A1, Glu-B1, Glu-B3 andGli-B1 loci was studied. Electrophoretic analyses ... cultivated wheats. Recombination between the Glu-B3 and Gli-B1 loci was observed. To determine ...
ABSTRACT A collection of 180 Triticum durum lines from Turkey and 22 Italian cultivars were inves... more ABSTRACT A collection of 180 Triticum durum lines from Turkey and 22 Italian cultivars were investigated by electrophoretic techniques and evaluated for grain parameters such as protein content and sodium dodecyl sulphate (SDS)-sedimentation test. A total of 14 different patterns were observed for high-molecular-weight (HMW) glutenin subunits resulting from the combination of three alleles from the A genome and seven from the B genome. Three new allelic variants, one at Glu-A1 and two at Glu-B1, were found. Five different alleles were detected at the Gli-B1 locus. Genotypes with the same allelic variant at Gli-B1 showed inconsistency of composition in B group of low-molecular-weight (LMW) glutenin subunits. A total of 11 different B-subunit patterns were identified in the material analysed. A substantial proportion (78%) of the variation in gluten properties could be explained in terms of protein composition, with the LMW glutenin subunits making the largest contribution (54%). Results indicate that, in addition to Glu-B3 encoded proteins, variation at other loci coding for some intermediate and fastest B subunits might also influence rheological properties.
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