Research Interests: Fungi, Multidisciplinary, Phylogeny, Animals, Applied Environmental Microbiology, and 15 moreBacteria, Netherlands, Phylogenetic analysis, Green Algae, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Ecosystem, Fresh water, Eukaryotic Cells, Fungal Diversity, Organic Matter, Community Composition, Food Chain, Landfill Leachate, Geologic Sediments, and Aem
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Research Interests: Numerical Analysis, Multidisciplinary, Phylogeny, Applied, Applied Environmental Microbiology, and 10 moreIron, Dissolved organic matter, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Ecosystem, Fresh water, Gene Targeting, Refuse disposal, Community Composition, Landfill Leachate, and Oxidation-Reduction
Investigation of renewable energy in Iowa is centering on the use of agricultural crops to generate electricity. Switchgrass, a native grass of Iowa, is one of the most promising biomass producers. Chariton Valley RC&D Inc., a USDA... more
Investigation of renewable energy in Iowa is centering on the use of agricultural crops to generate electricity. Switchgrass, a native grass of Iowa, is one of the most promising biomass producers. Chariton Valley RC&D Inc., a USDA affiliated rural development organization ...
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Research Interests: Microbial Ecology, Indonesia, Population Dynamics, Fungi, Multidisciplinary, and 15 moreApplied Environmental Microbiology, Bacillus, Polymerase Chain Reaction, High Temperature, Species Composition, Food Industry, Angiosperms, Electrophoresis, Ecosystem, Bacillus subtilis, Microbial Activity, Enzyme activity, Ribosomal DNA, Curing, and Microbial community
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Conceptually, growing switchgrass for biomass should be a straightforward farming endeavor, with agronomists simply adapting well-established production knowledge gathered from forage research. Pragmatically, this has not proven to be so... more
Conceptually, growing switchgrass for biomass should be a straightforward farming endeavor, with agronomists simply adapting well-established production knowledge gathered from forage research. Pragmatically, this has not proven to be so - at least not in the Chariton River Valley, which is a 160,000- hectare watershed in south central Iowa. The hilly, erosive landscapes consist of numerous soils containing high clay contents and typically having poor internal drainage and acidic sola ?1?. These landscape and soil limitations resulted in extensive land enrollment (i.e., about 12% of the watershed) in the USDA Conservation Reserve Program (CRP) and corollary establishment of switchgrass fields during the 1980's and 1990's ?see 2?. The objective of this paper is to document and discuss observed agronomic and environmental constraints in switchgrass production within the Chariton Valley. This objective is met by synthesizing results from two related on-farm studies. The first s...
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In the presence of different selection pressures, particularly pH and electron donor concentration, indigenous microbial associations which catabolize selected petroleum hydrocarbon components (benzene, toluene and o-, m- and p-xylene... more
In the presence of different selection pressures, particularly pH and electron donor concentration, indigenous microbial associations which catabolize selected petroleum hydrocarbon components (benzene, toluene and o-, m- and p-xylene (BTX)) were enriched and isolated from a petroleum hydrocarbon-contaminated KwaZulu-Natal sandy soil. Electron microscopy revealed that, numerically, rods constituted the majority of the populations responsible for BTX catabolism. Molecular techniques (polymerase chain reaction (PCR) and 16S rDNA fingerprinting by denaturing-gradient gel electrophoresis (DGGE)) were employed to explore the diversities and analyze the structures of the isolated microbial associations. Pearson product-moment correlation indicated that the different, but chemically similar, petroleum hydrocarbon molecules, effected the isolation of different associations. However, some similar numerically-dominant bands characterized the associations. A 30% similarity was evident between ...
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Databases containing information regarding presence and activity of microbial communities will be very useful for determination of the potential for intrinsic bioremediation in landfill leachate polluted aquifers. Simple analyses such as... more
Databases containing information regarding presence and activity of microbial communities will be very useful for determination of the potential for intrinsic bioremediation in landfill leachate polluted aquifers. Simple analyses such as community-level physiological profiling (CLPP) and denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments yield large sets of data for inclusion into such databases. In this study we describe the development of a method for anaerobic CLPP, using commercially available Biolog plates. Incubation at the in situ temperature of the aquifer (10 degrees C) for 28 days was optimal for obtaining a specific, reproducible physiological profile. Anaerobic incubation was essential for profiling anaerobic communities. The anaerobic cultivation-dependent CLPP method and cultivation-independent DGGE were applied to groundwater and sediment samples from the aquifer near the Coupépolder landfill in The Netherlands. A combination of computer-assisted CLP...
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Nitrosomonas europaea and Nitrobacter winogradskyi (strain "Engel") were grown in ammonia-limited and nitrite-limited conditions, respectively, in a retentostat with complete biomass retention at 25 degrees C and pH 8. Fitting... more
Nitrosomonas europaea and Nitrobacter winogradskyi (strain "Engel") were grown in ammonia-limited and nitrite-limited conditions, respectively, in a retentostat with complete biomass retention at 25 degrees C and pH 8. Fitting the retentostat biomass and oxygen consumption data of N. europaea and N. winogradskyi to the linear equation for substrate utilization resulted in up to eight-times-lower maintenance requirements compared to the maintenance energy demand (m) calculated from chemostat experiments. Independent of the growth rate at different stages of such a retention culture, the maximum specific oxygen consumption rate measured by mass spectrometric analysis of inlet and outlet gas oxygen content always amounted to approximately 45 micromol of O2 mg-1 of biomass-C x h-1 for both N. europaea and N. winogradskyi. When bacteria were starved for different time periods (up to 3 months), the spontaneous respiratory activity after an ammonia or nitrite pulse decreased with...
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Research Interests: Environmental Monitoring, Biodiversity, Biological Sciences, Environmental Sciences, Spatial Heterogeneity, and 12 moreAnimals, Bacteria, Netherlands, Soil Microbiology, Pulsed field gel electrophoresis (PFGE), Water Microbiology, Species Specificity, Quaternary Ammonium Compounds, Landfill Leachate, Electric Conductivity, Geologic Sediments, and Microbial community
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Research Interests: Fungi, Multidisciplinary, Phylogeny, Animals, Applied Environmental Microbiology, and 15 moreBacteria, Netherlands, Phylogenetic analysis, Green Algae, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Ecosystem, Fresh water, Eukaryotic Cells, Fungal Diversity, Organic Matter, Community Composition, Food Chain, Landfill Leachate, Geologic Sediments, and Aem
Research Interests: Numerical Analysis, Multidisciplinary, Phylogeny, Applied, Applied Environmental Microbiology, and 10 moreIron, Dissolved organic matter, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Ecosystem, Fresh water, Gene Targeting, Refuse disposal, Community Composition, Landfill Leachate, and Oxidation-Reduction
Research Interests: Biology, Water Pollution, Medicine, Multidisciplinary, Biodegradation, and 23 moreApplied, Archaea, Applied Environmental Microbiology, Bacteria, Drinking Water, Iron, Chemical Analysis, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Aquifers, Gram Positive Bacteria, Molecular cloning, Ecosystem, Tool Development, Fresh water, Leachate, Pollutant, Refuse disposal, Ribosomal DNA, Microbial Community Structure, Landfill Leachate, Aromatic Hydrocarbon, Geologic Sediments, and Microbial community
Research Interests: Microbial Ecology, Indonesia, Population Dynamics, Fungi, Multidisciplinary, and 16 moreApplied, Applied Environmental Microbiology, Bacillus, Polymerase Chain Reaction, High Temperature, Species Composition, Food Industry, Angiosperms, Electrophoresis, Ecosystem, Bacillus subtilis, Microbial Activity, Enzyme activity, Ribosomal DNA, Curing, and Microbial community
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Investigation of renewable energy in Iowa is centering on the use of agricultural crops to generate electricity. Switchgrass, a native grass of Iowa, is one of the most promising biomass producers. Chariton Valley RC&D Inc., a USDA... more
Investigation of renewable energy in Iowa is centering on the use of agricultural crops to generate electricity. Switchgrass, a native grass of Iowa, is one of the most promising biomass producers. Chariton Valley RC&D Inc., a USDA affiliated rural development organization ...