Papers by Mohammad Amoozegar
Microbiology, 2013
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Bioresource Technology, 2007
Studies were carried out on the decolorization of textile azo dyes by newly isolated halophilic a... more Studies were carried out on the decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Among the 27 strains of halophilic and halotolerant bacteria isolated from effluents of textile industries, three showed remarkable ability in decolorizing the widely utilized azo dyes. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that these strains belonged to the genus Halomonas. The three strains were able to decolorize azo dyes in a wide range of NaCl concentration (up to 20%w/v), temperature (25-40 degrees C), and pH (5-11) after 4 days of incubation in static culture. They could decolorize the mixture of dyes as well as pure dyes. These strains also readily grew in and decolorized the high concentrations of dye (5000 ppm) and could tolerate up to 10,000 ppm of the dye. UV-Vis analyses before and after decolorization and the colorless bacterial biomass after decolorization suggested that decolorization was due to biodegradation, rather than inactive surface adsorption. Analytical studies based on HPLC showed that the principal decolorization was reduction of the azo bond, followed by cleavage of the reduced bond.
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World Journal of Microbiology and Biotechnology, 2010
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Introduction: Dischargeing hazardous pollutants of oil and gas industries such as spent caustic i... more Introduction: Dischargeing hazardous pollutants of oil and gas industries such as spent caustic into the soil and water is an environmental concern for which biological treatment could offer a solution. To remove high levels of sulfur compounds in spent caustic waste, isolation of chemolithoautotrophic sulfur-oxidizing bacteria from Meighan wetland was considered in this study.
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J Ind Microbiol Biotechnol, 2009
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Fish & shellfish immunology, Jan 27, 2015
The present study investigates the effects of dietary supplements of galactooligosaccharides (GOS... more The present study investigates the effects of dietary supplements of galactooligosaccharides (GOS), Pediococcus acidilactici and P. acidilactici + GOS on innate immune response, skin mucus as well as disease resistance of rainbow trout (Oncorhynchus mykiss) fingerlings (15.04 ± 0.52 g). After 8 weeks of feeding, several innate immune (lysozyme, alternative complement and respiratory burst activities) and skin mucus parameters (bactericidal activity against Streptococcus faecium, Streptococcus iniae, Serratia marcescens, Staphylococcus aureus and Escherichia coli and mucus protein content) were studied. The results indicated that the three supplemented diet significantly increased innate immune response and skin mucus parameters in rainbow trout. The highest innate immune response, skin mucus activity as well as protein level was observed in synbiotic fed fish. Furthermore, at the end of the feeding experiment, some fish were intraperitoneally injected with Streptococcus iniae to det...
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International Journal of Environmental Science & Technology, 2011
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International Journal of Systematic and Evolutionary Microbiology, 2016
A novel Gram-staining-negative straight rod, non-pigmented, slightly halophilic and alkaliphilic ... more A novel Gram-staining-negative straight rod, non-pigmented, slightly halophilic and alkaliphilic bacterium, designated strain GBPy7T, was isolated from a sample of the coastal-marine wetland Gomishan in Iran. Cells of strain GBPy7T were motile. Growth occurred on media with 1-15 % (w/v) NaCl (optimum 3 % w/v), at pH 7-10 (optimum pH 8.5) and at 4-45 °C (optimum at 37 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that strain GBPy7T belonged to the family Idiomarinaceae. Its closest relatives were Aliidiomarina shirensis AIST (98.1 % sequence similarity) and other Aliidiomarina species (95.9-94.2 %), together with Idiomarina seosinensis CL-SP 19T (94.3 %) and Idiomarina fontislapidosi F23T (94.3 %). The major cellular fatty acids of the isolate were iso-C15:0, iso-C17:0, iso-C17:1ω9c and C18:1ω7c and its polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown aminophospholipid. The cells of strain GBPy7T contained ubiquinone Q-8. The G+C content of the genomic DNA of this strain was 51.6 mol %. The level of DNA-DNA relatedness between strain GBPy7T and Aliidiomarina shirensis IBRC-M 10414T was 21 %. The physiological, biochemical, genotypic and phylogenetic differences between strain GBPy7T and other previously described taxa indicate that the strain represents a novel species of the genus Aliidiomarina within the family Idiomarinaceae, for which the name Aliidiomarina iranensis sp. nov. is proposed, with strain GBPy7T (= IBRC-M 10763T = CECT 8339T) as the type strain.
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International Journal of Systematic and Evolutionary Microbiology, 2016
A moderately halophilic bacterium was isolated from a brine sample of hypersaline lake Aran-Bidgo... more A moderately halophilic bacterium was isolated from a brine sample of hypersaline lake Aran-Bidgol in Iran. The strain, designated J8BT, was Gram-staining-positive, endospore-forming, rod shaped, strictly aerobic, motile and produced colonies cream pigmented. Strain J8BT grew between 3.0-15.0 % (w/v) NaCl (optimum at 7.5 % w/v NaCl), between pH 6.5-9.0 (optimum at pH 8.0) and between 20-45 °C (optimum at 35 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain J8BT is a member of the genus Oceanobacillus and was most closely related to Oceanobacillus profundus CL-MP28T, Oceanobacillus polygoni SA9T and Oceanobacillus oncorhynchi R-2T (96.9 %, 96.3 %, and 96.2 % similarity, respectively). Level of DNA-DNA relatedness between the novel isolate and O. profundus IBRC-M 10567T was 10 %. The major cellular fatty acids of the isolate were anteiso-C15:0, iso-C15:0 and anteiso-C17:0. The polar lipids pattern of strain J8BT consisted of phosphatidylglycerol, diphosphatidylglycerol, five phospholipids, two aminolipids and two glycoaminolipids. It contained MK-7 as the predominant menaquinone and meso-diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 39.2 mol%. Phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data suggest that this strain represents a novel species of the genus Oceanobacillus, for which the name Oceanobacillus halophilus sp. nov. is proposed. The type strain is strain J8BT (= IBRC-M 10444 T= DSM 23996T).
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Antonie Van Leeuwenhoek Antonie Van Leeuwenhoek, Apr 12, 2013
A Gram-negative, non-endospore-forming, rod shaped, strictly aerobic, moderately halophilic bacte... more A Gram-negative, non-endospore-forming, rod shaped, strictly aerobic, moderately halophilic bacterium, designated strain M9B(T), was isolated from the hypersaline lake Aran-Bidgol in Iran. Cells of strain M9B(T) were found to be motile and produce colonies with an orange-yellow pigment. Growth was determined to occur between 5 and 20 % (w/v) NaCl and the isolate grew optimally at 7.5-10 % (v/w) NaCl. The optimum pH and temperature for growth of the strain were determined to be pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of 6-8 and 25-45 °C, respectively. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain M9B(T) is a member of the genus Marinobacter. The closest relative to this strain was found to be Marinobacter hydrocarbonoclasticus MBIC 1303(T) with a similarity level of 97.7 %. DNA-DNA hybridization between the novel isolate and this phylogenetically related species was 13 ± 2 %. The major cellular fatty acids of the isolate were identified as C16:0, C19:1 ω6c, C18:1 ω9c and C16:1 ω9c. The polar lipid pattern of strain M9B(T) was determined to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and three phospholipids. Ubiquinone 9 (Q-9) was the only lipoquinone detected. The G+C content of the genomic DNA of this strain was determined to be 58.6 mol%. Phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data suggest that this strain represents a novel species of the genus Marinobacter, for which the name Marinobacter persicus sp. nov. is proposed. The type strain of Marinobacter persicus is strain M9B(T) (=IBRC-M 10445(T) = CCM 7970(T) = CECT 7991(T) = KCTC 23561(T)).
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World Journal of Microbiology and Biotechnology, Sep 30, 2005
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Annals of Microbiology, 2011
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International Journal of Systematic and Evolutionary Microbiology, 2015
A novel orange pigmented halophilic archaeon, strain DC8T, was isolated from Urmia salt lake in N... more A novel orange pigmented halophilic archaeon, strain DC8T, was isolated from Urmia salt lake in North-West Iran. The cells of strain DC8T were non-motile and pleomorphic, from small rods to triangular or disk shaped. The novel strain needs at least 2.5 M NaCl and 0.02 M MgCl2 for growth. Optimal growth was achieved at 4.0 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 45 °C, respectively, and it was able to grow over a pH range of 7.0 to 8.5 and a temperature range of 25 to 55 °C. Analysis of the 16S rRNA gene sequence showed that strain DC8T was a member of the family Halobacteriaceae; however, its similarity was as low as 90.1 %, 89.3 % and 89.1 % to the most closely related haloarchaeal taxa including Halosimplex, Halobaculum and Halomicrobium type species, respectively. The G+C content of its DNA was 68.1 mol %. Polar lipid analyses revealed that strain DC8T contains phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulphate and phosphatidic acid. One unknown phospholipid, two major glycolipids and one minor glycolipid were also observed. The only quinone presented was MK-8 (ΙΙ-H2). The physiological, biochemical and phylogenetic differences between strain DC8T and other previously described extremely halophilic archaeal genera supported that this strain represents a novel species of a new genus within the family Halobacteriaceae, for which the name Halosiccatus urmianus gen. nov., sp. nov. is proposed. The type strain is strain DC8T (= IBRC-M 10911T= CECT 8793T).
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J Ind Microbiol Biotechnol, 2009
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Taxonomy and Biosystematics, Nov 15, 2013
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ABSTRACT Cucurbitariaceae is one of the newly introduced families of Pleosporales. Pyrenochaetops... more ABSTRACT Cucurbitariaceae is one of the newly introduced families of Pleosporales. Pyrenochaetopsis, Pyrenochaeta and Cucurbitaria are its mostly known members and some members of Phoma complex are their close relatives. The sequences of nrRNA gene including SSU, LSU and ITS and protein coding fragments (Tub and Act) of type strains (gained from qbank and CBS) were studied in the family. The information gathered from culture collections and biotechnology databases showed that species diversity of Cucurbitariaceae is more than that is understood and many isolates that are submitted to Genbank and culture collections may be identified and nominated not correctly. MLSA is a very resolving approach to identify higher and lower taxonomic levels for pleosporales that is examined here to identify the wild isolates of Cucurbitariaceae. It is shown that SSU is more than 99% conserved in Cucurbitariaceae and it could not resolve the species and even genera, although it is very useful in resolving higher taxonomic levels. Also sequence analysis of LSU, ITS, Act and Tub fragments showed that ITS is nearly 91-95% conserved in the family and has a very stronger database for phylogenetc investigations. Furthermore, MLSA results showed that the Pyrenochaeta species in core Cucurbitariaceae are not so different from the only member of Cucurbitaria. Interestingly, Herpotrichia parasitica has the potential to be transferred to Phaeosphaeriaceae.
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International Journal of Biological Macromolecules, 2016
Azo dyes are a major class of colorants used in various industries including textile, paper and f... more Azo dyes are a major class of colorants used in various industries including textile, paper and food. These dyes are regarded as pollutant since they are not readily reduced under aerobic conditions. Halomonas elongata, a halophilic bacterium, has the ability to decolorize different mono and di-azo dyes in anoxic conditions. In this study the putative azoreductase gene of H. elongata, formerly annotated as acp, was isolated, heterologously expressed in Escherichia coli, purified and characterized. The gene product, AzoH, was found to have a molecular mass of 22kDa. The enzyme requires NADH, as an electron donor for its activity. The apparent Km was 63μM for NADH and 12μM for methyl red as a mono-azo dye substrate. The specific activity for methyl red was 0.27μmolmin(-1)mg(-1). The optimum enzyme activity was achieved in 50mM sodium phosphate buffer at pH 6. Although increased salinity resulted in reduced activity, AzoH could decolorize azo dye at NaCl concentrations up to 15% (w/v). The enzyme was also shown to be able to decolorize remazol black B as a representative of di-azo dyes. This is the first report describing the sequence and activity of an azo-reducing enzyme from a halophilic bacterium.
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Papers by Mohammad Amoozegar