Functional convergence of CD28 costimulation and TCR signaling is critical to T-cell activation a... more Functional convergence of CD28 costimulation and TCR signaling is critical to T-cell activation and adaptive immunity. These receptors form complex microscale patterns within the immune synapse, although the impact of this spatial organization on cell signaling remains unclear. We investigate this cross talk using micropatterned surfaces that present ligands to these membrane proteins in order to control the organization of signaling molecules within the cell-substrate interface. While primary human CD4 + T cells were activated by features containing ligands to both CD3 and CD28, this functional convergence was curtailed on surfaces in which engagement of these two systems was separated by micrometer-scale distances. Moreover, phosphorylated Lck was concentrated to regions of CD3 engagement and exhibited a low diffusion rate, suggesting that costimulation is controlled by a balance between the transport of active Lck to CD28 and its deactivation. In support of this model, disruption...
The response of neural cells to chemical patterning is used to direct cell attachment and growth.... more The response of neural cells to chemical patterning is used to direct cell attachment and growth. Microscopic control of neuron placement on a surface is an essential component of our effort on live neural network computing. Control of attachment and growth of neurons and glia is also desirable for neurological implants and research on central nervous system function. Chemical patterning
Functional convergence of CD28 costimulation and TCR signaling is critical to T-cell activation a... more Functional convergence of CD28 costimulation and TCR signaling is critical to T-cell activation and adaptive immunity. These receptors form complex microscale patterns within the immune synapse, although the impact of this spatial organization on cell signaling remains unclear. We investigate this cross talk using micropatterned surfaces that present ligands to these membrane proteins in order to control the organization of signaling molecules within the cell-substrate interface. While primary human CD4 + T cells were activated by features containing ligands to both CD3 and CD28, this functional convergence was curtailed on surfaces in which engagement of these two systems was separated by micrometer-scale distances. Moreover, phosphorylated Lck was concentrated to regions of CD3 engagement and exhibited a low diffusion rate, suggesting that costimulation is controlled by a balance between the transport of active Lck to CD28 and its deactivation. In support of this model, disruption...
The response of neural cells to chemical patterning is used to direct cell attachment and growth.... more The response of neural cells to chemical patterning is used to direct cell attachment and growth. Microscopic control of neuron placement on a surface is an essential component of our effort on live neural network computing. Control of attachment and growth of neurons and glia is also desirable for neurological implants and research on central nervous system function. Chemical patterning
Uploads