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    Kiran Patil

    Osteoblastoma is a rare (incidence <1%) benign locally aggressive osteoblastic lesion of bone with rare malignant transformation. Benign osteoblastoma is the term used by Jaffe to denote an uncommon neoplasm of bone characterized by... more
    Osteoblastoma is a rare (incidence <1%) benign locally aggressive osteoblastic lesion of bone with rare malignant transformation. Benign osteoblastoma is the term used by Jaffe to denote an uncommon neoplasm of bone characterized by vascular osteoid and bone forming matrix rich in osteoblasts. Here we report a case of osteoblastoma of talus in eighteen year old male patient from Bijapur with complaints of pain and difficulty in walking for one year. Relevant investigations and radiographs were taken. It was treated with wide excision and bone grafting from ipsilateral ilium and talo-navicular fusion with cannulated cancellous screw fixation. Histopathology of the biopsy confirmed the diagnosis. Patient was immobilized with below knee plaster cast for six weeks later with gradual mobilization. Guarded weight bear was allowed after three months. Patient achieved satisfactory range of movements and full weight bear mobilization after six months. Symptoms relieved with no evidence of...
    RNA duplex regions are often involved in tertiary interactions and protein binding and thus there is great potential in developing ligands that sequence-specifically bind to RNA duplexes. We have developed a convenient synthesis method... more
    RNA duplex regions are often involved in tertiary interactions and protein binding and thus there is great potential in developing ligands that sequence-specifically bind to RNA duplexes. We have developed a convenient synthesis method for a modified peptide nucleic acid (PNA) monomer with a guanidine-modified 5-methyl cytosine base. We demonstrated by gel electrophoresis, fluorescence and thermal melting experiments that short PNAs incorporating the modified residue show high binding affinity and sequence specificity in the recognition of an RNA duplex containing an internal inverted Watson-Crick C-G base pair. Remarkably, the relatively short PNAs show no appreciable binding to DNA duplexes or single-stranded RNAs. The attached guanidine group stabilizes the base triple through hydrogen bonding with the G base in a C-G pair. Selective binding towards an RNA duplex over a single-stranded RNA can be rationalized by the fact that alkylation of the amine of a 5-methyl C base blocks th...
    The diversity of industrially important molecules for which microbial production routes have been experimentally demonstrated is rapidly increasing. The development of economically viable producer cells is, however, lagging behind, as it... more
    The diversity of industrially important molecules for which microbial production routes have been experimentally demonstrated is rapidly increasing. The development of economically viable producer cells is, however, lagging behind, as it requires substantial engineering of the host metabolism. A chassis strain suitable for production of a range of molecules is therefore highly sought after but remains elusive. Here, we propose a genome-scale metabolic modeling approach to design chassis strains of Saccharomyces cerevisiae - a widely used microbial cell factory. For a group of 29 products covering a broad range of biochemistry and applications, we identified modular metabolic engineering strategies for re-routing carbon flux towards the desired product. We find distinct product families with shared targets forming the basis for the corresponding chassis cells. The design strategies include overexpression targets that group products by similarity in precursor and cofactor requirements, as well as gene deletion strategies for growth-product coupling that lead to non-intuitive product groups. Our results reveal the extent and the nature of flux re-routing necessary for producing a diverse range of products in a widely used cell factory and provide blueprints for constructing pre-optimized chassis strains.
    ABSTRACT ABSTRACT This paper addresses an industrially important problem of recovery of acetic acid from a waste water streams via reactive distillation. The separation (or recovery) of organic residues from aqueous waste streams released... more
    ABSTRACT ABSTRACT This paper addresses an industrially important problem of recovery of acetic acid from a waste water streams via reactive distillation. The separation (or recovery) of organic residues from aqueous waste streams released from chemical and petrochemical industries is critical and indispensable from the points of view of pollution control and recovery of useful materials. The disposal of waste waters containing most widely-used industrial organic acids such as acetic acid, formic acid and propionic acid has been recognized as a significant expense to the industry and environment. In this paper, existing methods of physical separation of acetic acid from waste water is presented. Available conventional techniques including fractional distillation, liquid extraction, adsorption, precipitation, ion exchange, etc. have been briefly reviewed emphasizing the major drawbacks of these methods. A new method – reactive distillation - for recovering acetic acids from dilute aqueous solutions is described and compared with the other conventional techniques. Reactive distillation is an efficient, economical, and environmental friendly method for separation of acetic acids from waste water streams
    Arginine-rich cell penetrating peptides are powerful tools for in vitro as well as in vivo delivery of a wide plethora of biomolecules. However, presence of consecutive arginine residues leads to enhanced amenability for proteolytic... more
    Arginine-rich cell penetrating peptides are powerful tools for in vitro as well as in vivo delivery of a wide plethora of biomolecules. However, presence of consecutive arginine residues leads to enhanced amenability for proteolytic degradation as well as steric hindrances for membrane interactions which compromise its bioavailability. In order to overcome these limitations we previously reported a safe and stable octaarginine based oligomer, i.e., (r-x-r)4-carbamate, where the backbone amide linkages were replaced by carbamate linkages and 6-aminohexanoic acid based spacer moieties were incorporated for better flexibility, hydrophobicity, optimal spacing of guanidinium groups, and protection against proteolytic cleavage; resulting in improved transfection efficiency over its amide counterpart. In the present work we have investigated the mechanism behind this enhanced transfection efficiency and, based on our observations, demonstrate how the synergistic effect of rationalized olig...
    Page 1. Systems Biology of Metabolic Networks: Uncovering Regulatory and Stoichiometric Principles Kiran Raosaheb Patil ' Ph.D. thesis Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of... more
    Page 1. Systems Biology of Metabolic Networks: Uncovering Regulatory and Stoichiometric Principles Kiran Raosaheb Patil ' Ph.D. thesis Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark Page 2. PhD Thesis, Kiran R. Patil. ...
    ... 63, No. 9, pp. 881–887 (2008). [6]AS Birajdar, SN Meyyanathan, RB Raja, N. Krishanaveni, and B. Suresh, Acta Chromatographica 20(3), 411–421 (2008). ... and J. Pedrazolli Jr., Journal of Chromato graphy B 862, 1-2, 132-139 (2008). [9]... more
    ... 63, No. 9, pp. 881–887 (2008). [6]AS Birajdar, SN Meyyanathan, RB Raja, N. Krishanaveni, and B. Suresh, Acta Chromatographica 20(3), 411–421 (2008). ... and J. Pedrazolli Jr., Journal of Chromato graphy B 862, 1-2, 132-139 (2008). [9] Vijaya D. Bharathi, Kanbarpa Radharani ...
    Benzenediazonium sulfate (BD) was given to Swiss mice by 26 subcutaneous injections of 10 micrograms/g body weight at weekly intervals. The treatment gave rise to tumors of the subcutis. The tumor incidences in the treated groups were 42%... more
    Benzenediazonium sulfate (BD) was given to Swiss mice by 26 subcutaneous injections of 10 micrograms/g body weight at weekly intervals. The treatment gave rise to tumors of the subcutis. The tumor incidences in the treated groups were 42% in females and 26% in males. The corresponding tumor incidences in the untreated controls were 0% in females and 2% in males. Histopathologically, the neoplasms were classified as fibrosarcomas, rhabdomyosarcomas, and osteosarcomas of the subcutaneous tissue. BD is formed during the cytochrome P-450 catalyzed metabolism of the carcinogenic 1-(phenylazo)-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14), which was used as a coloring agent for food and other materials in several countries. Further, BD is a metabolic breakdown product of different classes of nitrogen-nitrogen bond- containing chemicals. BD is the fourth benzenediazonium salt found to be carcinogenic in this laboratory.
    The regulation of gene expression in response to nutrient availability is fundamental to the genotype-phenotype relationship. The metabolic-genetic make-up of the cell, as reflected in auxotrophy, is hence likely to be a determinant of... more
    The regulation of gene expression in response to nutrient availability is fundamental to the genotype-phenotype relationship. The metabolic-genetic make-up of the cell, as reflected in auxotrophy, is hence likely to be a determinant of gene expression. Here, we address the importance of the metabolic-genetic background by monitoring transcriptome, proteome and metabolome in a repertoire of 16 Saccharomyces cerevisiae laboratory backgrounds, combinatorially perturbed in histidine, leucine, methionine and uracil biosynthesis. The metabolic background affected up to 85% of the coding genome. Suggesting widespread confounding, these transcriptional changes show, on average, 83% overlap between unrelated auxotrophs and 35% with previously published transcriptomes generated for non-metabolic gene knockouts. Background-dependent gene expression correlated with metabolic flux and acted, predominantly through masking or suppression, on 88% of transcriptional interactions epistatically. As a consequence, the deletion of the same metabolic gene in a different background could provoke an entirely different transcriptional response. Propagating to the proteome and scaling up at the metabolome, metabolic background dependencies reveal the prevalence of metabolism-dependent epistasis at all regulatory levels. Urging a fundamental change of the prevailing laboratory practice of using auxotrophs and nutrient supplemented media, these results reveal epistatic intertwining of metabolism with gene expression on the genomic scale.
    ABSTRACT Advanced precision machinery, from centrifuges to computer disk drives, rely on precision bearing and spindle assemblies for high performance. The spacing of data tracks on a computer disk drive can be limited by the... more
    ABSTRACT Advanced precision machinery, from centrifuges to computer disk drives, rely on precision bearing and spindle assemblies for high performance. The spacing of data tracks on a computer disk drive can be limited by the non-repeatable runout of the spindle bearing assembly. For reasons like these, the need to measure runout and diagnose its cause has increased in recent years. Traditionally, runout has been measured with the electronic equivalent of a dial indicator and oscilloscopes which can determine the magnitude of runout. More recently, spectrum analyzers have been used, because they can help identify the various causes of runout by providing the frequency distribution information, as well as the data available from other testing methods. Originally confined to design labs, spectrum analyzers are now finding their way into incoming inspection and onto the manufacturing floor, where they are used to measure changes in runout caused by critical assembly steps. This paper explores the advantages of using a dynamic signal analyzer to make runout measurements; using both the traditional time domain measurements, as well as spectrum measurements. The measurements shown were made on a disk memory spindle assembly. Also the correlation between the time domain NRR measurement and the asynchronous runout measurement is also discussed.
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    ABSTRACT This work explores the synthesis of iso-amyl acetate via reactive distillation. In this work the experimental studies on continuous RD experiments on laboratory cum pilot scale. This work comprehensively reports on both... more
    ABSTRACT This work explores the synthesis of iso-amyl acetate via reactive distillation. In this work the experimental studies on continuous RD experiments on laboratory cum pilot scale. This work comprehensively reports on both experiments and simulation. The experimental part includes reactive distillation experiments in Laboratory scale, carefully evaluated database for describing chemical reaction and phase equilibria. The equilibrium model for synthesis of iso amyl acetate w as developed. The simulation studies were performed in Aspen Plus and MATLAB. The Published experimental data on phase equilibrium and reaction studies was used to verify the model predictions The simulations are based on the equilibrium-stage approach and, for comparison; a rate-based model is also used. The experimental results were compared with model predictions and were found to be close to those available in the literature. It was observed that as boil-up increases, the amount of iso amyl alcohol, which is excess in the feed, increases in top product thereby improving the purity of the bottom product. As anticipated, the time required to attain the steady state was more for lower boil-up rate.
    Page 1. A Stability-Indicating LC Method for the Simultaneous Determination of Telmisartan and Ramipril in Dosage Form Kiran R. Patil1,2, Vipul P. Rane1,2, Jaiprakash N. Sangshetti1, Devanand B. Shinde1,& 1 Department ...

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