... (1999) and higher than Suzuki et al. (1992), Madan et al. (1994a), Kaddoom (1995), Abbas (199... more ... (1999) and higher than Suzuki et al. (1992), Madan et al. (1994a), Kaddoom (1995), Abbas (1998) and Ganguli et al. (1998). ... References. Abbas, 1998 Abbas, HS, 1998. Investigations on in-vitro fertilization in buffaloes. PhD thesis (Theriogenology). Faculty of Vet. Med. ...
The present work is undertaken mainly for genotyping of kappa-casein gene in buffalo bulls. DNA w... more The present work is undertaken mainly for genotyping of kappa-casein gene in buffalo bulls. DNA was extracted from the frozen semen of nine buffalo bulls used in artificial insemination in addition to 40 blood samples of male buffalo calves for identification and genotyping of kappa-casein gene by PCR-RFLP assay. Different restriction endonucleases, including HindIII, HinfI and Taq1 were used. The PCR product of the primer specific for K2 and KY gives the specific band at size 379 and 280Â bp respectively. Digestion of 379Â bp fragment K2 by restriction endonuclease HindIII generated two fragments of 156 and 223Â bp. For fragment 280Â bp kappa-casein KY, the above mentioned endonuclease generated two fragments of 180 and 100Â bp. Digestion of the 379Â bp PCR product with HinfI resulted two restriction fragments of 288 and 91Â bp. On the other hand, the amplified DNA (379Â bp) from buffalo remained undigested by Taq1 restriction enzyme.In conclusion, the PCR-RFLP technique demonstrated that bulls were monomorphic for the kappa-casein gene; they possess only allele B in homozygosis form.
Asian Journal of Animal and Veterinary Advances, 2008
... Moreover, increased in structural chromosomal aberrations and no increase in numerical aberra... more ... Moreover, increased in structural chromosomal aberrations and no increase in numerical aberrations in trypanosomosis camel. ... Thus, chromosomal abnormalities and SCE`S may be implicated in the pathogenesis of trypanosomosis. ...
... (1999) and higher than Suzuki et al. (1992), Madan et al. (1994a), Kaddoom (1995), Abbas (199... more ... (1999) and higher than Suzuki et al. (1992), Madan et al. (1994a), Kaddoom (1995), Abbas (1998) and Ganguli et al. (1998). ... References. Abbas, 1998 Abbas, HS, 1998. Investigations on in-vitro fertilization in buffaloes. PhD thesis (Theriogenology). Faculty of Vet. Med. ...
The present work is undertaken mainly for genotyping of kappa-casein gene in buffalo bulls. DNA w... more The present work is undertaken mainly for genotyping of kappa-casein gene in buffalo bulls. DNA was extracted from the frozen semen of nine buffalo bulls used in artificial insemination in addition to 40 blood samples of male buffalo calves for identification and genotyping of kappa-casein gene by PCR-RFLP assay. Different restriction endonucleases, including HindIII, HinfI and Taq1 were used. The PCR product of the primer specific for K2 and KY gives the specific band at size 379 and 280Â bp respectively. Digestion of 379Â bp fragment K2 by restriction endonuclease HindIII generated two fragments of 156 and 223Â bp. For fragment 280Â bp kappa-casein KY, the above mentioned endonuclease generated two fragments of 180 and 100Â bp. Digestion of the 379Â bp PCR product with HinfI resulted two restriction fragments of 288 and 91Â bp. On the other hand, the amplified DNA (379Â bp) from buffalo remained undigested by Taq1 restriction enzyme.In conclusion, the PCR-RFLP technique demonstrated that bulls were monomorphic for the kappa-casein gene; they possess only allele B in homozygosis form.
Asian Journal of Animal and Veterinary Advances, 2008
... Moreover, increased in structural chromosomal aberrations and no increase in numerical aberra... more ... Moreover, increased in structural chromosomal aberrations and no increase in numerical aberrations in trypanosomosis camel. ... Thus, chromosomal abnormalities and SCE`S may be implicated in the pathogenesis of trypanosomosis. ...
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