This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DE... more This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DEP) platform for the separation and detection of the human leukemic cancer cells (K562) and its multidrug resistant (MDR) counterpart (K56/IMA-0.2 µM) from the human leukocyte mixture. The pro-posed DEP device, benefits from the 3D isolated electrodes inside a microfluidic channel. 3D elec-trodes (extruded 30 µm in z-direction) were isolated from the medium with thin parylene coating (~ 0.3 µm). In addition, the potentially-floating 3D elec-trodes were utilized to enhance the DEP separation efficiency, for the first time in the literature. These electrodes, elevates the performance of the device by extending the generated electric field gradient inside the DEP separation area. FEM electric field and incompressible Navier-Stoke analyses were utilized to study the separation efficiency of the proposed device. It is shown that under the optimized boundary conditions, the proposed device can ...
This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DE... more This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DEP) platform for the separation and detection of the human leukemic cancer cells (K562) and its multidrug resistant (MDR) counterpart (K56/IMA-0.2 µM) from the human leukocyte mixture. The pro-posed DEP device, benefits from the 3D isolated electrodes inside a microfluidic channel. 3D elec-trodes (extruded 30 µm in z-direction) were isolated from the medium with thin parylene coating (~ 0.3 µm). In addition, the potentially-floating 3D elec-trodes were utilized to enhance the DEP separation efficiency, for the first time in the literature. These electrodes, elevates the performance of the device by extending the generated electric field gradient inside the DEP separation area. FEM electric field and incompressible Navier-Stoke analyses were utilized to study the separation efficiency of the proposed device. It is shown that under the optimized boundary conditions, the proposed device can ...
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Papers by Kamal Al-hadad