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Gwo JC

    Gwo JC

    Several critical variables associated with successful cryopreservation of oyster embryos (Crassostrea gigas) were examined. These were 1) embryo developmental stage, 2) kind and concentration of cryoprotectant, 3) equilibration time, and... more
    Several critical variables associated with successful cryopreservation of oyster embryos (Crassostrea gigas) were examined. These were 1) embryo developmental stage, 2) kind and concentration of cryoprotectant, 3) equilibration time, and 4) freezing rate. The percentage of survival was scored as the number of recovered embryos that swam actively 12 h after thawing and had developed into veliger stage. The oyster
    ... Reports of successful freezing of viable Rainbow Trout (Oncorhynchus mykiss=Salmo gairdneri) eggs to -55ºC (Zell 1978) or to -20ºC (Erdahl and Graham 1980) were subsequently interpreted as supercooling (Harvey and Ashwood-Smith 1982).... more
    ... Reports of successful freezing of viable Rainbow Trout (Oncorhynchus mykiss=Salmo gairdneri) eggs to -55ºC (Zell 1978) or to -20ºC (Erdahl and Graham 1980) were subsequently interpreted as supercooling (Harvey and Ashwood-Smith 1982). ...