Morphology and postnatal development of the porcine lung are described in animals ranging in age ... more Morphology and postnatal development of the porcine lung are described in animals ranging in age from newborn through 60 days. Standardized fixation was accomplished by intratracheal instillation of glutaraldehyde under constant pressure. Light microscopic, scanning, and transmission electron microscopic investigations revealed that the porcine lung follows the common architecture of mammalian lungs, but has certain peculiarities as well: intravascular macrophages, ultrastructurally similar to Kupffer cells, are attached to endothelial cells in pulmonary capillaries and are involved in erythrophagocytosis during the first postnatal weeks. Type II pneumocytes of newborn pigs exhibit signs of cell activation, mainly complex nuclear bodies in the cell nuclei. At the same time high levels of 17-hydroxycorticosteroids are observed in the newborn blood plasma. Terminal airways of the porcine lung are nonalveolarized and are, therefore, of purely conductive function. At birth the porcine l...
Alveolar regions of normal pig lungs (newborn to 60-day-old) were characterized morphometrically ... more Alveolar regions of normal pig lungs (newborn to 60-day-old) were characterized morphometrically to provide a basis for comparison in future investigations of porcine respiratory diseases. Endotracheal installation of fixative was done to expand the lungs uniformly at total capacity. Differential effects of lobar variations were determined by stratified random sampling of lung lobes. A stereologic study was done by point and intersection counts on electron micrographs. At birth, the lungs were remarkably well developed. Relative alveolar and capillary surface densities and air-blood tissue barrier thicknesses were at adult levels. In allometric regressions, volumes and surfaces of lung components regressed directly to lung volume, but monoexponentially (to the 3/4 power) with body weight. In the first postnatal week, however, relative volume densities of cellular interstitium in septal tissue and of capillary lumina in parenchyma increased at statistically significant levels. Compos...
Ultrastructural studies of near-term to 2-month-old pigs were done to document characteristics an... more Ultrastructural studies of near-term to 2-month-old pigs were done to document characteristics and developmental changes of intravascular macrophages in pulmonary capillaries. Evidence is presented that blood monocytes colonize the porcine lung perinatally, replicate within capillaries postnatally, and attach to endothelium by intercellular junctions during differentiation. Major ultrastructural features of differentiated intravascular macrophages are adhesion to capillary endothelium, pseudopods, phagosomes, and tubular structures of micropinocytosis vermiformis. Ultrastructure indicates that intravascular macrophages are cells of the mononuclear phagocyte system involved in several functions (eg, blood cell sequestration) that are usually attributed to hepatolineal macrophages. In newborn and 3-day-old pigs, the majority of cells closely apposed to endothelium consisted of few differentiated monocytes, but in 7-day-old and older animals, most cells that were joined to endothelium ...
In dogs, laboratory animals, and man, the clearance of bacteria and particulates from blood occur... more In dogs, laboratory animals, and man, the clearance of bacteria and particulates from blood occurs predominantly in hepatic Kupffer cells and splenic macrophages. In contrast, removal of blood-borne particulates in calves, sheep, goats, cats, and pigs occurs predominantly in pulmonary intravascular macrophages (PIMs). Review of recent studies indicates that PIMs are a resident cell population, junctionally adherent to the capillary endothelium of lungs and morphologically similar to hepatic Kupffer cells. PIMs are a pulmonary constituent of the mononuclear phagocyte system with respect to secretory, endocytic, and functional properties. Differentiated PIMs are rare in newborn pigs, and the majority of cells closely apposed to capillary endothelium consists of monocytes, which are occasionally in mitosis. In 7-day-old and older pigs, most cells apposed to capillary endothelium have characteristics of differentiated PIMs. This suggests a monocytic origin of PIMs in pigs. Perinatal col...
Experimental infection of specific-pathogen-free pigs with swine influenza virus by the intratrac... more Experimental infection of specific-pathogen-free pigs with swine influenza virus by the intratracheal route resulted in a severe respiratory disease that closely resembled natural swine influenza in clinical course and pathologic lesions. Alveolar epithelial necrosis with sloughing of necrotic cells occurred from 24 to 96 hours after inoculation (p.i.) and was associated with alveolar edema and diffuse interstitial pneumonitis. The latter, initially of neutrophilic character, became histiocytic 48 hours p.i. Ultrastructural analysis of alveolar parenchyma disclosed viral replication in epithelial cells beginning at 5 hours p.i. and lasting to 96 hours. Budding of pleomorphic virus particles from the surface of alveolar epithelial cells and accumulation of viral proteins within the nucleus and cytoplasm of epithelial cells were seen. The extent of parenchymal lesions as quantified by stereologic morphometry within the whole lung was characterized by a marked relative and absolute vol...
Pulmonary intravascular macrophages (PIMs) have been recognized as the site of substantial uptake... more Pulmonary intravascular macrophages (PIMs) have been recognized as the site of substantial uptake of blood-borne particles in the lungs of a number of domestic animal species. Concomitantly, there is a pronounced lung susceptibility to endotoxin in calves, goats, sheep, pigs, and cats. Hemodynamic changes and initial lung injury after endotoxin administration are mediated by arachidonic acid metabolites from a pulmonary source. A significant role of PIMs in regulating pulmonary hemodynamics is implicated.
Lungs of newborn and of 7- and 30-day-old pigs were fixed at total capacity by a standardized pro... more Lungs of newborn and of 7- and 30-day-old pigs were fixed at total capacity by a standardized procedure. Stereologic morphometry was applied to random ultrathin sections of parenchyma. Relative volume density, absolute volume, and capillary surface coverage of intravascular macrophages as well as parameters of relative morphometric diffusion capacity of lungs were assessed. Intravascular macrophages occupied up to 25% of capillary volume in 30-day-old pigs compared with 6% in newborn pigs; this was a 14-fold increase of absolute volume. In 30-day-old pigs, 16% of capillary surface was covered with closely adherent intravascular macrophages in contrast to only 2% in newborn pigs. Determinators of morphometric diffusion capacity were similar in all pigs. Greater thickness of the arithmetic and harmonic mean air-blood tissue barrier in 30-day-old pigs (with included thickness of intravascular macrophages) compared with air-blood tissue barriers (without intravascular macrophages) was not statistically significant. The relative difference of arithmetic mean was larger than the difference of harmonic mean, and reflected preferential adherence of intravascular macrophages to the thick portion of the air-blood tissue barrier. Our findings confirm morphologic observations of perinatal colonization of the porcine lung by monocytes that replicate and differentiate into large, highly phagocytic, resident, intravascular macrophages. Preferential adherence to sites where basal laminae of capillary endothelium and alveolar epithelium were separated by interstitium may explain the minimal morphometric impact of intravascular macrophages on the air-blood tissue barrier thickness. This location would be expected to have less effect on gas diffusion than location of intravascular macrophages on the thin side of the air-blood barrier.
ABSTRACT Brain cortex neurons from adult mammals contain amounts of DNA elevated to between the d... more ABSTRACT Brain cortex neurons from adult mammals contain amounts of DNA elevated to between the diploid and tetraploid level. In the rat, the rise in DNA starts rapidly a few hours before birth, then slows down to taper off at 3 weeks after birth. In view of the possibility that the extra amount of DNA may reflect the specific amplification of a restricted set of functionally important genes, we have searched for qualitative differences distinguishing the extra DNA of rat cortex neurons from other somatic DNA, the latter being represented by prenatally synthesized neuronal DNA and liver DNA. However, all DNAs examined were found to be indistinguishable by multiple criteria, such as base composition, secondary structure content, appearance of native and heat-denatured DNA in the electron microscope, and proportion of sequences with varying degrees of repetition. Further, no evidence for selective gene amplification was obtained. Thus, at the present level of analysis the postnatally synthesized extra DNA of cortex neurons seems not to differ qualitatively from other somatic DNA.
Serum samples from 1421 domestic cats (561 healthy, 860 sick) were tested for FIV-, FeLV- and cor... more Serum samples from 1421 domestic cats (561 healthy, 860 sick) were tested for FIV-, FeLV- and coronavirus infection. The results were stored in a computer data base and compared with epidemiologic data and clinical findings. All 3 infections were significantly more prevalent in sick than healthy cats: FIV was found in 0.7% of healthy and 3.4% of sick cats. For FeLV the prevalence was 3.0% and 13.0% and for coronavirus 21% and 36.2%, respectively. FIV-infected cats were mostly male (73%); no sex predilection was observed in FeLV- and coronavirus infection. In sick cats FIV-infection was significantly more prevalent in cats greater than 2 years of age; no age-dependence was found in FeLV- and coronavirus infections. The prevalence of FIV-infection increased significantly with the number of animals per household. In contrast, the frequency of FeLV infection decreased with the number of animals per household. Prevalence of coronavirus infection did not vary with group size or living con...
Morphology and postnatal development of the porcine lung are described in animals ranging in age ... more Morphology and postnatal development of the porcine lung are described in animals ranging in age from newborn through 60 days. Standardized fixation was accomplished by intratracheal instillation of glutaraldehyde under constant pressure. Light microscopic, scanning, and transmission electron microscopic investigations revealed that the porcine lung follows the common architecture of mammalian lungs, but has certain peculiarities as well: intravascular macrophages, ultrastructurally similar to Kupffer cells, are attached to endothelial cells in pulmonary capillaries and are involved in erythrophagocytosis during the first postnatal weeks. Type II pneumocytes of newborn pigs exhibit signs of cell activation, mainly complex nuclear bodies in the cell nuclei. At the same time high levels of 17-hydroxycorticosteroids are observed in the newborn blood plasma. Terminal airways of the porcine lung are nonalveolarized and are, therefore, of purely conductive function. At birth the porcine l...
Alveolar regions of normal pig lungs (newborn to 60-day-old) were characterized morphometrically ... more Alveolar regions of normal pig lungs (newborn to 60-day-old) were characterized morphometrically to provide a basis for comparison in future investigations of porcine respiratory diseases. Endotracheal installation of fixative was done to expand the lungs uniformly at total capacity. Differential effects of lobar variations were determined by stratified random sampling of lung lobes. A stereologic study was done by point and intersection counts on electron micrographs. At birth, the lungs were remarkably well developed. Relative alveolar and capillary surface densities and air-blood tissue barrier thicknesses were at adult levels. In allometric regressions, volumes and surfaces of lung components regressed directly to lung volume, but monoexponentially (to the 3/4 power) with body weight. In the first postnatal week, however, relative volume densities of cellular interstitium in septal tissue and of capillary lumina in parenchyma increased at statistically significant levels. Compos...
Ultrastructural studies of near-term to 2-month-old pigs were done to document characteristics an... more Ultrastructural studies of near-term to 2-month-old pigs were done to document characteristics and developmental changes of intravascular macrophages in pulmonary capillaries. Evidence is presented that blood monocytes colonize the porcine lung perinatally, replicate within capillaries postnatally, and attach to endothelium by intercellular junctions during differentiation. Major ultrastructural features of differentiated intravascular macrophages are adhesion to capillary endothelium, pseudopods, phagosomes, and tubular structures of micropinocytosis vermiformis. Ultrastructure indicates that intravascular macrophages are cells of the mononuclear phagocyte system involved in several functions (eg, blood cell sequestration) that are usually attributed to hepatolineal macrophages. In newborn and 3-day-old pigs, the majority of cells closely apposed to endothelium consisted of few differentiated monocytes, but in 7-day-old and older animals, most cells that were joined to endothelium ...
In dogs, laboratory animals, and man, the clearance of bacteria and particulates from blood occur... more In dogs, laboratory animals, and man, the clearance of bacteria and particulates from blood occurs predominantly in hepatic Kupffer cells and splenic macrophages. In contrast, removal of blood-borne particulates in calves, sheep, goats, cats, and pigs occurs predominantly in pulmonary intravascular macrophages (PIMs). Review of recent studies indicates that PIMs are a resident cell population, junctionally adherent to the capillary endothelium of lungs and morphologically similar to hepatic Kupffer cells. PIMs are a pulmonary constituent of the mononuclear phagocyte system with respect to secretory, endocytic, and functional properties. Differentiated PIMs are rare in newborn pigs, and the majority of cells closely apposed to capillary endothelium consists of monocytes, which are occasionally in mitosis. In 7-day-old and older pigs, most cells apposed to capillary endothelium have characteristics of differentiated PIMs. This suggests a monocytic origin of PIMs in pigs. Perinatal col...
Experimental infection of specific-pathogen-free pigs with swine influenza virus by the intratrac... more Experimental infection of specific-pathogen-free pigs with swine influenza virus by the intratracheal route resulted in a severe respiratory disease that closely resembled natural swine influenza in clinical course and pathologic lesions. Alveolar epithelial necrosis with sloughing of necrotic cells occurred from 24 to 96 hours after inoculation (p.i.) and was associated with alveolar edema and diffuse interstitial pneumonitis. The latter, initially of neutrophilic character, became histiocytic 48 hours p.i. Ultrastructural analysis of alveolar parenchyma disclosed viral replication in epithelial cells beginning at 5 hours p.i. and lasting to 96 hours. Budding of pleomorphic virus particles from the surface of alveolar epithelial cells and accumulation of viral proteins within the nucleus and cytoplasm of epithelial cells were seen. The extent of parenchymal lesions as quantified by stereologic morphometry within the whole lung was characterized by a marked relative and absolute vol...
Pulmonary intravascular macrophages (PIMs) have been recognized as the site of substantial uptake... more Pulmonary intravascular macrophages (PIMs) have been recognized as the site of substantial uptake of blood-borne particles in the lungs of a number of domestic animal species. Concomitantly, there is a pronounced lung susceptibility to endotoxin in calves, goats, sheep, pigs, and cats. Hemodynamic changes and initial lung injury after endotoxin administration are mediated by arachidonic acid metabolites from a pulmonary source. A significant role of PIMs in regulating pulmonary hemodynamics is implicated.
Lungs of newborn and of 7- and 30-day-old pigs were fixed at total capacity by a standardized pro... more Lungs of newborn and of 7- and 30-day-old pigs were fixed at total capacity by a standardized procedure. Stereologic morphometry was applied to random ultrathin sections of parenchyma. Relative volume density, absolute volume, and capillary surface coverage of intravascular macrophages as well as parameters of relative morphometric diffusion capacity of lungs were assessed. Intravascular macrophages occupied up to 25% of capillary volume in 30-day-old pigs compared with 6% in newborn pigs; this was a 14-fold increase of absolute volume. In 30-day-old pigs, 16% of capillary surface was covered with closely adherent intravascular macrophages in contrast to only 2% in newborn pigs. Determinators of morphometric diffusion capacity were similar in all pigs. Greater thickness of the arithmetic and harmonic mean air-blood tissue barrier in 30-day-old pigs (with included thickness of intravascular macrophages) compared with air-blood tissue barriers (without intravascular macrophages) was not statistically significant. The relative difference of arithmetic mean was larger than the difference of harmonic mean, and reflected preferential adherence of intravascular macrophages to the thick portion of the air-blood tissue barrier. Our findings confirm morphologic observations of perinatal colonization of the porcine lung by monocytes that replicate and differentiate into large, highly phagocytic, resident, intravascular macrophages. Preferential adherence to sites where basal laminae of capillary endothelium and alveolar epithelium were separated by interstitium may explain the minimal morphometric impact of intravascular macrophages on the air-blood tissue barrier thickness. This location would be expected to have less effect on gas diffusion than location of intravascular macrophages on the thin side of the air-blood barrier.
ABSTRACT Brain cortex neurons from adult mammals contain amounts of DNA elevated to between the d... more ABSTRACT Brain cortex neurons from adult mammals contain amounts of DNA elevated to between the diploid and tetraploid level. In the rat, the rise in DNA starts rapidly a few hours before birth, then slows down to taper off at 3 weeks after birth. In view of the possibility that the extra amount of DNA may reflect the specific amplification of a restricted set of functionally important genes, we have searched for qualitative differences distinguishing the extra DNA of rat cortex neurons from other somatic DNA, the latter being represented by prenatally synthesized neuronal DNA and liver DNA. However, all DNAs examined were found to be indistinguishable by multiple criteria, such as base composition, secondary structure content, appearance of native and heat-denatured DNA in the electron microscope, and proportion of sequences with varying degrees of repetition. Further, no evidence for selective gene amplification was obtained. Thus, at the present level of analysis the postnatally synthesized extra DNA of cortex neurons seems not to differ qualitatively from other somatic DNA.
Serum samples from 1421 domestic cats (561 healthy, 860 sick) were tested for FIV-, FeLV- and cor... more Serum samples from 1421 domestic cats (561 healthy, 860 sick) were tested for FIV-, FeLV- and coronavirus infection. The results were stored in a computer data base and compared with epidemiologic data and clinical findings. All 3 infections were significantly more prevalent in sick than healthy cats: FIV was found in 0.7% of healthy and 3.4% of sick cats. For FeLV the prevalence was 3.0% and 13.0% and for coronavirus 21% and 36.2%, respectively. FIV-infected cats were mostly male (73%); no sex predilection was observed in FeLV- and coronavirus infection. In sick cats FIV-infection was significantly more prevalent in cats greater than 2 years of age; no age-dependence was found in FeLV- and coronavirus infections. The prevalence of FIV-infection increased significantly with the number of animals per household. In contrast, the frequency of FeLV infection decreased with the number of animals per household. Prevalence of coronavirus infection did not vary with group size or living con...
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