Our strategy of probes designing for major clones of Trypanosoma cruzi was performed taking into ... more Our strategy of probes designing for major clones of Trypanosoma cruzi was performed taking into account the: (i) clear identification of the major clones under multilocus study; (ii) hypothesis of a parallel evolution between the extranuclear and nuclear markers; (iii) structure of kDNA which allowed to amplify high variable regions of the minicircle (HVRm) by PCR. The large production of HVRm was very useful to test their ability to be used as probes for detection of DNA from a diversified genetic panel of T. cruzi. Our success in designing such probes has important implications on: the enhancement of 2 evolutive hypothesis about the clonal structure of T. cruzi and the parallel evolution of their extranuclear and nuclear genetics markers; direct diagnosis in patients and vectors. Studies on bio-clinical significance of major clones are discussed. This procedure could be used as a general strategy to generate DNA probes for Kinetoplastida.
Although the frequency of infection with the human immunodeficiency virus (HIV) is increasing dra... more Although the frequency of infection with the human immunodeficiency virus (HIV) is increasing dramatically in areas where Trypanosoma cruzi is endemic, trypanosomiasis has been rarely reported in persons with HIV infection or AIDS. Persons with hemophilia who receive multiple blood product transfusions from blood banks with little or no screening for infectious agents are at particularly high risk for infections with both HIV and T. cruzi. We describe the case of a person with hemophilia who was infected by blood transfusion with HIV and T. cruzi and in whom a multifocal, necrotic trypanosomal encephalitis was demonstrated by brain biopsy and electron microscopy. Treatment with benznidazole followed by that with itraconazole and fluconazole was associated with significant clinical and radiographic improvement.
Nutritionnal status remain poor in hospitalized elderly for cardiopathy compared to healthy contr... more Nutritionnal status remain poor in hospitalized elderly for cardiopathy compared to healthy controls, secondary to bad general status. Selenium rate appears significantly lower in ischemic cardiomyopathy than in valvular or hypertension cardiopathy.
... Aspects particuliers au sujet par P. LEDERLIN~, F. W~TZ, J.-P. AYMARD, J.-N. CoeomB, G.THIBAU... more ... Aspects particuliers au sujet par P. LEDERLIN~, F. W~TZ, J.-P. AYMARD, J.-N. CoeomB, G.THIBAUT, Oi GUErCl ... TOll BH, ROBERTS-THOMSON IC, MATHEWS JD, SENGA WITTINGHAM and MACKAY IR : Depression of cell-mediated immunity in old age and the immunopathic ...
Our strategy of probes designing for major clones of Trypanosoma cruzi was performed taking into ... more Our strategy of probes designing for major clones of Trypanosoma cruzi was performed taking into account the: (i) clear identification of the major clones under multilocus study; (ii) hypothesis of a parallel evolution between the extranuclear and nuclear markers; (iii) structure of kDNA which allowed to amplify high variable regions of the minicircle (HVRm) by PCR. The large production of HVRm was very useful to test their ability to be used as probes for detection of DNA from a diversified genetic panel of T. cruzi. Our success in designing such probes has important implications on: the enhancement of 2 evolutive hypothesis about the clonal structure of T. cruzi and the parallel evolution of their extranuclear and nuclear genetics markers; direct diagnosis in patients and vectors. Studies on bio-clinical significance of major clones are discussed. This procedure could be used as a general strategy to generate DNA probes for Kinetoplastida.
Although the frequency of infection with the human immunodeficiency virus (HIV) is increasing dra... more Although the frequency of infection with the human immunodeficiency virus (HIV) is increasing dramatically in areas where Trypanosoma cruzi is endemic, trypanosomiasis has been rarely reported in persons with HIV infection or AIDS. Persons with hemophilia who receive multiple blood product transfusions from blood banks with little or no screening for infectious agents are at particularly high risk for infections with both HIV and T. cruzi. We describe the case of a person with hemophilia who was infected by blood transfusion with HIV and T. cruzi and in whom a multifocal, necrotic trypanosomal encephalitis was demonstrated by brain biopsy and electron microscopy. Treatment with benznidazole followed by that with itraconazole and fluconazole was associated with significant clinical and radiographic improvement.
Nutritionnal status remain poor in hospitalized elderly for cardiopathy compared to healthy contr... more Nutritionnal status remain poor in hospitalized elderly for cardiopathy compared to healthy controls, secondary to bad general status. Selenium rate appears significantly lower in ischemic cardiomyopathy than in valvular or hypertension cardiopathy.
... Aspects particuliers au sujet par P. LEDERLIN~, F. W~TZ, J.-P. AYMARD, J.-N. CoeomB, G.THIBAU... more ... Aspects particuliers au sujet par P. LEDERLIN~, F. W~TZ, J.-P. AYMARD, J.-N. CoeomB, G.THIBAUT, Oi GUErCl ... TOll BH, ROBERTS-THOMSON IC, MATHEWS JD, SENGA WITTINGHAM and MACKAY IR : Depression of cell-mediated immunity in old age and the immunopathic ...
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